7VVO: PTH-bound human PTH1R

PTH-bound human PTH1R in complex with Gs (class5). Determined by electron microscopy at 4.1 Å resolution. Released 3 Aug 2022.

Method: Electron microscopy
Resolution: 4.1 Å
Organisms: Homo sapiens, Rattus norvegicus, Bos taurus
Chains: 6
Atoms: 7,942
Mol. weight: 163.95 kDa
Released: 3 Aug 2022

Explore 7VVO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7VVO contains 37 α-helices and 57 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	14-39	26
β-strand	41-46	6	1
α-helix	53-59	7
β-strand	208-210	3	1
β-strand	213-214	2	2
β-strand	217-218	2	2
β-strand	219-223	5	1
α-helix	235-237	3
β-strand	243-249	7	1
α-helix	253-267	15
β-strand	276-282	7	1
α-helix	284-293	10
α-helix	298-300	3
α-helix	303-307	5
α-helix	309-311	3
α-helix	316-317	2
α-helix	322-342	21
β-strand	349	1	1
β-strand	352-353	2	1
α-helix	363-380	18

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	38-39	2
β-strand	47	1	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	102-105	4	5
β-strand	111-115	5	5
β-strand	122-125	4	5
β-strand	135-137	3	5
β-strand	149-151	3	6
β-strand	156-159	4	6
β-strand	166-170	5	6
β-strand	175-180	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	304-308	5	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	337-339	3	3

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	8-22	15
α-helix	30-43	14
α-helix	53-55	3

Chain N: 2 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	3-5	3	10
β-strand	11-12	2	11
β-strand	19-25	7	10
α-helix	29-31	3
β-strand	35-39	5	12
β-strand	45-50	6	12
β-strand	60	1	12
β-strand	65	1	10
β-strand	68-73	6	10
β-strand	78-83	6	10
α-helix	88-90	3
β-strand	92-97	6	12
β-strand	118	1	12
β-strand	122-124	3	12
β-strand	125-126	2	11

Chain R: 18 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	34-51	18
β-strand	127-130	4	13
α-helix	131-132	2
β-strand	143-146	4	13
β-strand	148	1	14
β-strand	154	1	14
α-helix	155	1
β-strand	156	1	15
α-helix	157	1
α-helix	162	1
β-strand	163	1	15
α-helix	164	1
β-strand	166	1	13
α-helix	187-210	24
α-helix	218-240	23
α-helix	282-284	3
α-helix	288-308	21
α-helix	322-327	6
α-helix	333-341	9
α-helix	342-346	5
α-helix	361-390	30
α-helix	399-417	19
α-helix	419-422	4
α-helix	443-459	17
α-helix	464-476	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	380	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	68	Bos taurus	P63212 (AlphaFold model)
nanobody Nb35	N	protein	137	unidentified
Parathyroid hormone	P	protein	34	Homo sapiens	P01270 (AlphaFold model)
Parathyroid hormone/parathyroid hormone-related peptide receptor	R	protein	473	Homo sapiens	Q03431

Sequence of entity 1 (A), FASTA

>7VVO_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
GNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYH
VNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPEN
QFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDV
IKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAI
IFVVDSSDYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPE
FARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARR
IFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>7VVO_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>7VVO_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFS

Sequence of entity 4 (N), FASTA

>7VVO_4 nanobody Nb35 (chains N)
MGQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASI
SYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYR
GQGTQVTVSSLHHHHHH

Sequence of entity 5 (P), FASTA

>7VVO_5 Parathyroid hormone (chains P)
SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNF

Sequence of entity 6 (R), FASTA

>7VVO_6 Parathyroid hormone/parathyroid hormone-related peptide receptor (chains R)
DYKDDDDKDADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKP
RKDKASGKLYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDF
NHKGHAYRRCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLGMIYTVGYSVS
LASLTVAVLILAYFRRLHCTRNYIHMHLFLSFMLRAVSIFVKDAVLYSGATLDEAERLTE
EELRAIAQAPPPPATAAAGYAGCRVAVTFFLYFLATNYYWILVEGLYLHSLIFMAFFSEK
KYLWGFTVFGWGLPAVFVAVWVSVRATLANTGCWDLSSGNKKWIIQVPILASIVLNFILF
INIVRVLATKLRETNAGRCDTRQQYRKLLKSTLVLMPLFGVHYIVFMATPYTEVSGTLWQ
VQMHYEMLFNSFQGFFVAIIYCFCNGEVQAEIKKSWSRWTLALDFKRKARSGS

Primary citation

Endogenous ligand recognition and structural transition of a human PTH receptor. Kobayashi, K., Kawakami, K., Kusakizako, T. et al. Mol Cell (2022) 82:3468-3483.e5. DOI 10.1016/j.molcel.2022.07.003 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

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