7X2U: Sodium/hydrogen exchanger 3

Structure of a human NHE3-CHP1 complex in the autoinhibited state. Determined by electron microscopy at 3.2 Å resolution. Released 20 Apr 2022.

Method
Electron microscopy
Resolution
3.2 Å
Organism
Homo sapiens
Chains
4
Atoms
11,861
Mol. weight
193.03 kDa
Ligands
PGT, 85R
Released
20 Apr 2022

Explore 7X2U in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7X2U contains 100 α-helices and 12 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 35 helices, 1 β-strand

ElementResiduesLengthSheet
α-helix54-7219
α-helix76-794
α-helix84-10118
α-helix105-1073
α-helix112-1143
α-helix115-1195
α-helix120-12910
α-helix133-1386
α-helix140-1445
α-helix145-1495
α-helix150-16718
α-helix178-18811
α-helix193-1964
α-helix197-2004
α-helix207-23731
α-helix244-27532
α-helix276-2783
α-helix286-30318
α-helix308-31710
α-helix329-35931
α-helix368-39427
α-helix403-4108
α-helix417-4248
α-helix434-44613
α-helix447-4526
α-helix453-46311
α-helix475-49420
α-helix501-51010
α-helix511-5155
α-helix516-5194
α-helix522-53615
α-helix618-6236
β-strand62411
α-helix625-6273
α-helix628-6303
α-helix646-66015
Chain B: 35 helices, 1 β-strand
ElementResiduesLengthSheet
α-helix54-7219
α-helix76-794
α-helix84-10118
α-helix105-1073
α-helix112-1143
α-helix115-1195
α-helix120-12910
α-helix133-1386
α-helix140-1445
α-helix145-1495
α-helix150-16718
α-helix178-18811
α-helix193-1964
α-helix197-2004
α-helix207-23731
α-helix244-27532
α-helix276-2783
α-helix286-30318
α-helix308-31710
α-helix329-35830
α-helix368-39427
α-helix403-4108
α-helix417-4248
α-helix434-44613
α-helix447-4526
α-helix453-46311
α-helix475-49420
α-helix501-51010
α-helix511-5155
α-helix516-5194
α-helix522-53615
α-helix618-6236
β-strand62412
α-helix625-6273
α-helix628-6303
α-helix646-66015
Chains C and D: 15 helices, 5 β-strands
ElementResiduesLengthSheet
α-helix13-219
α-helix25-3814
β-strand4613
α-helix48-525
α-helix63-708
α-helix721
β-strand7813
α-helix80-878
α-helix88-903
α-helix92-943
α-helix95-984
α-helix111-12212
β-strand129-13024
α-helix132-14110
β-strand14311
α-helix149-16315
β-strand171-17224
α-helix173-1797
α-helix180-1823
α-helix185-1884

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Sodium/hydrogen exchanger 3A, Bprotein626Homo sapiensP48764 (AlphaFold model)
Calcineurin B homologous protein 1C, Dprotein185Homo sapiensQ99653 (AlphaFold model)
Sequence of entity 1 (A, B), FASTA
>7X2U_1 Sodium/hydrogen exchanger 3 (chains A, B)
GGFQVVTFEWAHVQDPYVIALWILVASLAKIGFHLSHKVTSVVPESALLIVLGLVLGGIV
WAADHIASFTLTPTVFFFYLLPPIVLDAGYFMPNRLFFGNLGTILLYAVVGTVWNAATTG
LSLYGVFLSGLMGDLQIGLLDFLLFGSLMAAVDPVAVLAVFEEVHVNEVLFIIVFGESLL
NDAVTVVLYNVFESFVALGGDNVTGVDCVKGIVSFFVVSLGGTLVGVVFAFLLSLVTRFT
KHVRIIEPGFVFIISYLSYLTSEMLSLSAILAITFCGICCQKYVKANISEQSATTVRYTM
KMLASSAETIIFMFLGISAVNPFIWTWNTAFVLLTLVFISVYRAIGVVLQTWLLNRYRMV
QLEPIDQVVLSYGGLRGAVAFALVVLLDGDKVKEKNLFVSTTIIVVFFTVIFQGLTIKPL
VQWLKVKRSEHREPRLNEKLHGRAFDHILSAIEDISGQIGHNYLRDKWSHFDRKFLSRVL
MRRSAQKSRDRILNVFHELNLKDAISYVAEGERRGSLAFIRSPSTDNVVNVDFTPRSSTV
EASVSYLLRENVSAVCLDMQSLEQRRRSIRDAEDMVTHHTLQQYLYKPRQEYKHLYSRHE
LTPTEDEKQDREIFHRTMRKRLESFK
Sequence of entity 2 (C, D), FASTA
>7X2U_2 Calcineurin B homologous protein 1 (chains C, D)
DEELEEIKKETGFSHSQITRLYSRFTSLDKGENGTLSREDFQRIPELAINPLGDRIINAF
FPEGEDQVNFRGFMRTLAHFRPIEDNEKSKDVNGPEPLNSRSNKLHFAFRLYDLDKDEKI
SRDELLQVLRMMVGVNISDEQLGSIADRTIQEADQDGDSIASFTEFVKVLEKVDVEQKMS
IRFLH

Ligands and cofactors

IDNameFormulaCopies
PGT(1S)-2-{[{[(2R)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoylo…C40 H79 O10 P10
85R[(2~{R})-2-hexadecanoyloxy-3-[oxidanyl-[(2~{S},3~{S},5~{R},6~{S})-2,3,4,5,6-pen…C41 H79 O13 P2

Primary citation

Structure of a human NHE3-CHP1 complex in the autoinhibited state. Dong, Y., Li, H., Ilie, A. et al. Sci Adv (2022).

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