7XHO: Human inner kinetochore CCAN complex
Structure of human inner kinetochore CCAN complex. Determined by electron microscopy at 3.29 Å resolution. Released 14 Dec 2022.
- Method
- Electron microscopy
- Resolution
- 3.29 Å
- Organism
- Homo sapiens
- Chains
- 17
- Atoms
- 22,243
- Mol. weight
- 720.49 kDa
- Released
- 14 Dec 2022
Explore 7XHO in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7XHO contains 125 α-helices and 73 β-strands across 16 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain c: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 263-267 | 5 | |
Chain H: 7 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 40-65 | 26 | |
| α-helix | 78-81 | 4 | |
| α-helix | 83-86 | 4 | |
| α-helix | 88-115 | 28 | |
| α-helix | 126-187 | 62 | |
| α-helix | 195-209 | 15 | |
| α-helix | 231-236 | 6 | |
Chain I: 34 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 63-74 | 12 | |
| α-helix | 85-98 | 14 | |
| α-helix | 103-111 | 9 | |
| α-helix | 123-130 | 8 | |
| β-strand | 137 | 1 | 1 |
| α-helix | 139-146 | 8 | |
| α-helix | 158-170 | 13 | |
| α-helix | 171-173 | 3 | |
| β-strand | 175 | 1 | 1 |
| α-helix | 178-182 | 5 | |
| α-helix | 185-189 | 5 | |
| α-helix | 190-193 | 4 | |
| α-helix | 198-208 | 11 | |
| α-helix | 211-213 | 3 | |
| α-helix | 216-228 | 13 | |
| α-helix | 233-244 | 12 | |
| α-helix | 267-281 | 15 | |
| α-helix | 370-377 | 8 | |
| α-helix | 382-401 | 20 | |
| α-helix | 402-404 | 3 | |
| α-helix | 413-428 | 16 | |
| α-helix | 433-442 | 10 | |
| α-helix | 452-458 | 7 | |
| α-helix | 467-470 | 4 | |
| α-helix | 471-475 | 5 | |
| α-helix | 487-513 | 27 | |
| α-helix | 530-550 | 21 | |
| α-helix | 555-565 | 11 | |
| α-helix | 569-571 | 3 | |
| α-helix | 572-575 | 4 | |
| α-helix | 585-592 | 8 | |
| α-helix | 597-620 | 24 | |
| α-helix | 635-650 | 16 | |
| α-helix | 686-694 | 9 | |
| α-helix | 716-725 | 10 | |
| α-helix | 729-735 | 7 | |
Chain K: 7 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 21-45 | 25 | |
| α-helix | 55-76 | 22 | |
| α-helix | 90-146 | 57 | |
| α-helix | 155-182 | 28 | |
| α-helix | 184-187 | 4 | |
| α-helix | 209-222 | 14 | |
| α-helix | 240-243 | 4 | |
| β-strand | 253-254 | 2 | 2 |
| β-strand | 262-263 | 2 | 2 |
Chain L: 13 helices, 19 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 28-39 | 12 | |
| β-strand | 47-48 | 2 | 3 |
| α-helix | 49-51 | 3 | |
| α-helix | 61-68 | 8 | |
| β-strand | 72-77 | 6 | 4 |
| β-strand | 80-81 | 2 | 5 |
| α-helix | 88-104 | 17 | |
| β-strand | 118-123 | 6 | 4 |
| α-helix | 134-136 | 3 | |
| β-strand | 137-142 | 6 | 4 |
| β-strand | 156 | 1 | 4 |
| β-strand | 160-163 | 4 | 4 |
| α-helix | 168-171 | 4 | |
| β-strand | 178-179 | 2 | 5 |
| β-strand | 183-186 | 4 | 4 |
| α-helix | 190-204 | 15 | |
| β-strand | 208-210 | 3 | 4 |
| α-helix | 211 | 1 | |
| α-helix | 215-225 | 11 | |
| β-strand | 237-242 | 6 | 6 |
| β-strand | 251-256 | 6 | 6 |
| α-helix | 258-268 | 11 | |
| β-strand | 274-275 | 2 | 3 |
| α-helix | 277-295 | 19 | |
| β-strand | 297 | 1 | 7 |
| α-helix | 299-301 | 3 | |
| β-strand | 303-308 | 6 | 6 |
| β-strand | 313 | 1 | 8 |
| β-strand | 314-315 | 2 | 6 |
| β-strand | 319-321 | 3 | 4 |
| β-strand | 322 | 1 | 8 |
| α-helix | 328-343 | 16 | |
Chain M: 7 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 15 | 1 | 9 |
| β-strand | 18-21 | 4 | 10 |
| α-helix | 25-37 | 13 | |
| β-strand | 43 | 1 | 9 |
| β-strand | 47-49 | 3 | 10 |
| β-strand | 65-68 | 4 | 10 |
| α-helix | 75-82 | 8 | |
| α-helix | 85-87 | 3 | |
| β-strand | 97-98 | 2 | 10 |
| β-strand | 99-101 | 3 | 11 |
| α-helix | 114-123 | 10 | |
| β-strand | 128-130 | 3 | 11 |
| α-helix | 136-138 | 3 | |
| α-helix | 140-153 | 14 | |
| α-helix | 162-168 | 7 | |
Chain N: 11 helices, 14 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-13 | 8 | |
| α-helix | 22-27 | 6 | |
| α-helix | 33-38 | 6 | |
| α-helix | 45-57 | 13 | |
| α-helix | 63-75 | 13 | |
| β-strand | 83-89 | 7 | 12 |
| α-helix | 101-115 | 15 | |
| β-strand | 120-122 | 3 | 12 |
| β-strand | 125-126 | 2 | 12 |
| β-strand | 130-136 | 7 | 12 |
| β-strand | 150-155 | 6 | 12 |
| β-strand | 160-164 | 5 | 12 |
| α-helix | 172-182 | 11 | |
| β-strand | 187-190 | 4 | 12 |
| β-strand | 195 | 1 | 12 |
| α-helix | 198-206 | 9 | |
| β-strand | 238-239 | 2 | 10 |
| α-helix | 242-255 | 14 | |
| β-strand | 267-270 | 4 | 6 |
| β-strand | 273 | 1 | 13 |
| β-strand | 291 | 1 | 13 |
| β-strand | 293-298 | 6 | 6 |
| α-helix | 302-307 | 6 | |
| β-strand | 315 | 1 | 7 |
| α-helix | 321-324 | 4 | |
Chain O: 10 helices, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 96-99 | 4 | |
| α-helix | 102-110 | 9 | |
| β-strand | 114-116 | 3 | 14 |
| β-strand | 123-129 | 7 | 14 |
| β-strand | 134-141 | 8 | 14 |
| β-strand | 142 | 1 | 15 |
| β-strand | 150 | 1 | 15 |
| α-helix | 161-168 | 8 | |
| α-helix | 177-191 | 15 | |
| α-helix | 193-199 | 7 | |
| β-strand | 205 | 1 | 16 |
| β-strand | 210 | 1 | 17 |
| α-helix | 213-215 | 3 | |
| β-strand | 217-221 | 5 | 17 |
| β-strand | 222 | 1 | 16 |
| β-strand | 233-238 | 6 | 17 |
| β-strand | 249-254 | 6 | 17 |
| α-helix | 262-277 | 16 | |
| α-helix | 280-282 | 3 | |
| α-helix | 284-290 | 7 | |
| α-helix | 292-294 | 3 | |
8 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Centromere protein C | C, c | protein | 943 | Homo sapiens | Q03188 (AlphaFold model) |
| Centromere protein H | H | protein | 247 | Homo sapiens | Q9H3R5 (AlphaFold model) |
| Centromere protein I | I | protein | 756 | Homo sapiens | Q92674 (AlphaFold model) |
| Centromere protein K | K | protein | 269 | Homo sapiens | Q9BS16 (AlphaFold model) |
| Centromere protein L | L | protein | 344 | Homo sapiens | Q8N0S6 |
| Centromere protein M | M | protein | 180 | Homo sapiens | Q9NSP4 |
| Centromere protein N | N | protein | 339 | Homo sapiens | Q96H22 |
| Centromere protein O | O | protein | 300 | Homo sapiens | Q9BU64 |
| Centromere protein P | P | protein | 288 | Homo sapiens | Q6IPU0 |
| Centromere protein Q | Q | protein | 268 | Homo sapiens | Q7L2Z9 |
| Centromere protein S | S | protein | 138 | Homo sapiens | Q8N2Z9 |
| Centromere protein T | T | protein | 561 | Homo sapiens | Q96BT3 |
4 more molecules are not listed.
Sequence of entity 1 (C, c), FASTA
>7XHO_1 Centromere protein C (chains C, c)
MAASGLDHLKNGYRRRFCRPSRARDINTEQGQNVLEILQDCFEEKSLANDFSTNSTKSVP
NSTRKIKDTCIQSPSKECQKSHPKSVPVSSKKKEASLQFVVEPSEATNRSVQAHEVHQKI
LATDVSSKNTPDSKKISSRNINDHHSEADEEFYLSVGSPSVLLDAKTSVSQNVIPSSAQK
RETYTFENSVNMLPSSTEVSVKTKKRLNFDDKVMLKKIEIDNKVSDEEDKTSEGQERKPS
GSSQNRIRDSEYEIQRQAKKSFSTLFLETVKRKSESSPIVRHAATAPPHSCPPDDTKLIE
DEFIIDESDQSFASRSWITIPRKAGSLKQRTISPAESTALLQGRKSREKHHNILPKTLAN
DKHSHKPHPVETSQPSDKTVLDTSYALIGETVNNYRSTKYEMYSKNAEKPSRSKRTIKQK
QRRKFMAKPAEEQLDVGQSKDENIHTSHITQDEFQRNSDRNMEEHEEMGNDCVSKKQMPP
VGSKKSSTRKDKEESKKKRFSSESKNKLVPEEVTSTVTKSRRISRRPSDWWVVKSEESPV
YSNSSVRNELPMHHNSSRKSTKKTNQSSKNIRKKTIPLKRQKTATKGNQRVQKFLNAEGS
GGIVGHDEISRCSLSEPLESDEADLAKKKNLDCSRSTRSSKNEDNIMTAQNVPLKPQTSG
YTCNIPTESNLDSGEHKTSVLEESGPSRLNNNYLMSGKNDVDDEEVHGSSDDSKQSKVIP
KNRIHHKLVLPSNTPNVRRTKRTRLKPLEYWRGERIDYQGRPSGGFVISGVLSPDTISSK
RKAKENIGKVNKKSNKKRICLDNDERKTNLMVNLGIPLGDPLQPTRVKDPETREIILMDL
VRPQDTYQFFVKHGELKVYKTLDTPFFSTGKLILGPQEEKGKQHVGQDILVFYVNFGDLL
CTLHETPYILSTGDSFYVPSGNYYNIKNLRNEESVLLFTQIKR
Sequence of entity 2 (H), FASTA
>7XHO_2 Centromere protein H (chains H)
MEEQPQMQDADEPADSGGEGRAGGPPQVAGAQAACSEDRMTLLLRLRAQTKQQLLEYKSM
VDASEEKTPEQIMQEKQIEAKIEDLENEIEEVKVAFEIKKLALDRMRLSTALKKNLEKIS
RQSSVLMDNMKHLLELNKLIMKSQQESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKN
KQKIDLDSMENSERIKIIRQNLQMEIKITTVIQHVFQNLILGSKVNWAEDPALKEIVLQL
EKNVDMM
Sequence of entity 3 (I), FASTA
>7XHO_3 Centromere protein I (chains I)
MSPQKRVKNVQAQNRTSQGSSSFQTTLSAWKVKQDPSNSKNISKHGQNNPVGDYEHADDQ
AEEDALQMAVGYFEKGPIKASQNKDKTLEKHLKTVENVAWKNGLASEEIDILLNIALSGK
FGNAVNTRILKCMIPATVISEDSVVKAVSWLCVGKCSGSTKVLFYRWLVAMFDFIDRKEQ
INLLYGFFFASLQDDALCPYVCHLLYLLTKKENVKPFRVRKLLDLQAKMGMQPHLQALLS
LYKFFAPALISVSLPVRKKIYFKNSENLWKTALLAVKQRNRGPSPEPLKLMLGPANVRPL
KRKWNSLSVIPVLNSSSYTKECGKKEMSLSDCLNRSGSFPLEQLQSFPQLLQNIHCLELP
SQMGSVLNNSLLLHYINCVRDEPVLLRFYYWLSQTLQEECIWYKVNNYEHGKEFTNFLDT
IIRAECFLQEGFYSCEAFLYKSLPLWDGLCCRSQFLQLVSWIPFSSFSEVKPLLFDHLAQ
LFFTSTIYFKCSVLQSLKELLQNWLLWLSMDIHMKPVTNSPLETTLGGSMNSVSKLIHYV
GWLSTTAMRLESNNTFLLHFILDFYEKVCDIYINYNLPLVVLFPPGIFYSALLSLDTSIL
NQLCFIMHRYRKNLTAAKKNELVQKTKSEFNFSSKTYQEFNHYLTSMVGCLWTSKPFGKG
IYIDPEILEKTGVAEYKNSLNVVHHPSFLSYAVSFLLQESPEERTVNVSSIRGKKWSWYL
DYLFSQGLQGLKLFIRSSVHHSSIPRAEGINCNNQY
Sequence of entity 4 (K), FASTA
>7XHO_4 Centromere protein K (chains K)
MNQEDLDPDSTTDVGDVTNTEEELIRECEEMWKDMEECQNKLSLIGTETLTDSNAQLSLL
IMQVKCLTAELSQWQKKTPETIPLTEDVLITLGKEEFQKLRQDLEMVLSTKESKNEKLKE
DLEREQRWLDEQQQIMESLNVLHSELKNKVETFSESRIFNELKTKMLNIKEYKEKLLSTL
GEFLEDHFPLPDRSVKKKKKNIQESSVNLITLHEMLEILINRLFDVPHDPYVKISDSFWP
PYVELLLRNGIALRHPEDPTRIRLEAFHQ
Sequence of entity 5 (L), FASTA
>7XHO_5 Centromere protein L (chains L)
MDSYSAPESTPSASSRPEDYFIGATPLQKRLESVRKQSSFILTPPRRKIPQCSQLQEDVD
PQKVAFLLHKQWTLYSLTPLYKFSYSNLKEYSRLLNAFIVAEKQKGLAVEVGEDFDIKVI
FSTLLGMKGTQRDPEAFLVQIVSKSQLPSENREGKVLWTGWFCCVFGDSLLETVSEDFTC
LPLFLANGAESNTAIIGTWFQKTFDCYFSPLAINAFNLSWMAAMWTACKMDHYVATTEFL
WSVPCSPQSLDISFAIHPEDAKALWDSVHKTPGEVTQEEVDLFMDCLYSHFHRHFKIHLS
ATRLVRVSTSVASAHTDGKIKILCHKYLIGVLAYLTELAIFQIE
Sequence of entity 6 (M), FASTA
>7XHO_6 Centromere protein M (chains M)
MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNR
PRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKL
AHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL
Sequence of entity 7 (N), FASTA
>7XHO_7 Centromere protein N (chains N)
MDETVAEFIKRTILKIPMNELTTILKAWDFLSENQLQTVNFRQRKESVVQHLIHLCEEKR
ASISDAALLDIIYMQFHQHQKVWDVFQMSKGPGEDVDLFDMKQFKNSFKKILQRALKNVT
VSFRETEENAVWIRIAWGTQYTKPNQYKPTYVVYYSQTPYAFTSSSMLRRNTPLLGQALT
IASKHHQIVKMDLRSRYLDSLKAIVFKQYNQTFETHNSTTPLQERSLGLDINMDSRIIHE
NIVEKERVQRITQETFGDYPQPQLEFAQYKLETKFKSGLNGSILAEREEPLRCLIKFSSP
HLLEALKSLAPAGIADAPLSPLLTCIPNKRMNYFKIRDK
Sequence of entity 8 (O), FASTA
>7XHO_8 Centromere protein O (chains O)
MEQANPLRPDGESKGGVLAHLERLETQVSRSRKQSEELQSVQAQEGALGTKIHKLRRLRD
ELRAVVRHRRASVKACIANVEPNQTVEINEQEALEEKLENVKAILQAYHFTGLSGKLTSR
GVCVCISTAFEGNLLDSYFVDLVIQKPLRIHHHSVPVFIPLEEIAAKYLQTNIQHFLFSL
CEYLNAYSGRKYQADRLQSDFAALLTGPLQRNPLCNLLSFTYKLDPGGQSFPFCARLLYK
DLTATLPTDVTVTCQGVEVLSTSWEEQRASHETLFCTKPLHQVFASFTRKGEKLDMSLVS
Sequence of entity 9 (P), FASTA
>7XHO_9 Centromere protein P (chains P)
MDAELAEVRALQAEIAALRRACEDPPAPWEEKSRVQKSFQAIHQFNLEGWKSSKDLKNQL
GHLESELSFLSTLTGINIRNHSKQTEDLTSTEMTEKSIRKVLQRHRLSGNCHMVTFQLEF
QILEIQNKERLSSAVTDLNIIMEPTECSELSEFVSRAEERKDLFMFFRSLHFFVEWFEYR
KRTFKHLKEKYPDAVYLSEGPSSCSMGIRSASRPGFELVIVWRIQIDEDGKVFPKLDLLT
KVPQRALELDKNRAIETAPLSFRTLVGLLGIEAALESLIKSLCAEENN
Sequence of entity 10 (Q), FASTA
>7XHO_10 Centromere protein Q (chains Q)
MSGKANASKKNAQQLKRNPKRKKDNEEVVLSENKVRNTVKKNKNHLKDLSSEGQTKHTNL
KHGKTAASKRKTWQPLSKSTRDHLQTMMESVIMTILSNSIKEKEEIQYHLNFLKKRLLQQ
CETLKVPPKKMEDLTNVSSLLNMERARDKANEEGLALLQEEIDKMVETTELMTGNIQSLK
NKIQILASEVEEEEERVKQMHQINSSGVLSLPELSQKTLKAPTLQKEILALIPNQNALLK
DLDILHNSSQMKSMSTFIEEAYKKLDAS
Sequence of entity 11 (S), FASTA
>7XHO_11 Centromere protein S (chains S)
MEEEAETEEQQRFSYQQRLKAAVHYTVGCLCEEVALDKEMQFSKQTIAAISELTFRQCEN
FAKDLEMFARHAKRTTINTEDVKLLARRSNSLLKYITDKSEEIAQINLERKAQKKKKSED
GSKNSRQPAEAGVVESEN
Sequence of entity 12 (T), FASTA
>7XHO_12 Centromere protein T (chains T)
MADHNPDSDSTPRTLLRRVLDTADPRTPRRPRSARAGARRALLETASPRKLSGQTRTIAR
GRSHGARSVGRSAHIQASGHLEEQTPRTLLKNILLTAPESSILMPESVVKPVPAPQAVQP
SRQESSCGSLELQLPELEPPTTLAPGLLAPGRRKQRLRLSVFQQGVDQGLSLSQEPQGNA
DASSLTRSLNLTFATPLQPQSVQRPGLARRPPARRAVDVGAFLRDLRDTSLAPPNIVLED
TQPFSQPMVGSPNVYHSLPCTPHTGAEDAEQAAGRKTQSSGPGLQKNSPGKPAQFLAGEA
EEVNAFALGFLSTSSGVSGEDEVEPLHDGVEEAEKKMEEEGVSVSEMEATGAQGPSRVEE
AEGHTEVTEAEGSQGTAEADGPGASSGDEDASGRAASPESASSTPESLQARRHHQFLEPA
PAPGAAVLSSEPAEPLLVRHPPRPRTTGPRPRQDPHKAGLSHYVKLFSFYAKMPMERKAL
EMVEKCLDKYFQHLCDDLEVFAAHAGRKTVKPEDLELLMRRQGLVTDQVSLHVLVERHLP
LEYRQLLIPCAYSGNSVFPAQ
Primary citation
Structural insights into human CCAN complex assembled onto DNA. Tian, T., Chen, L., Dou, Z. et al. Cell Discov (2022) 8:90-90. DOI 10.1038/s41421-022-00439-6 · PubMed
Other PDB entries of the same protein (UniProt Q03188 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7R5R 2.44 Å, Structure of the human CCAN CENP-A alpha-satellite complex
- 7PII 2.68 Å, Structure of the human CCAN CENP-A alpha-satellite complex
- 28OP 2.7 Å, Structure of the human inner kinetochore CCAN and CENP-C bound to DNA
- 5LSJ 3.25 Å, Crystal structure of the human kinetochore MIS12-cenp-C delta-HEAD2 complex
- 6MUP 3.5 Å, CENP-A nucleosome bound by two copies of CENP-C(CD) and two copies CENP-N(NT)
- 6SE6 3.5 Å, Class2 : CENP-A nucleosome in complex with CENP-C central region
- 5LSK 3.5 Å, Crystal structure of the human kinetochore MIS12-cenp-C complex
- 9TAW 3.54 Å, Structure of the human inner kinetochore CCAN bound to DNA
- 6MUO 3.6 Å, CENP-A nucleosome bound by two copies of CENP-C(CD) and one copy CENP-N(NT)
- 6SEF 3.7 Å, Class2C : CENP-A nucleosome in complex with CENP-C central region
- 7XHN 3.71 Å, Structure of human inner kinetochore CCAN-DNA complex
- 6SEE 4.2 Å, Class2A : CENP-A nucleosome in complex with CENP-C central region
Browse structure collections
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