7Z13: DNA replication licensing factor MCM2
S. cerevisiae CMGE dimer nucleating origin DNA melting. Determined by electron microscopy at 3.4 Å resolution. Released 15 Jun 2022.
- Method
- Electron microscopy
- Resolution
- 3.4 Å
- Organisms
- Saccharomyces cerevisiae, DNA molecule
- Chains
- 28
- Atoms
- 107,381
- Mol. weight
- 2297.54 kDa
- Ligands
- ATP, ZN, MG, ADP
- Released
- 15 Jun 2022
Explore 7Z13 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7Z13 contains 610 α-helices and 542 β-strands across 26 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chains 2 and a: 34 helices, 30 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 194-199 | 6 | |
| α-helix | 203-217 | 15 | |
| α-helix | 226-237 | 12 | |
| β-strand | 241-245 | 5 | 1 |
| α-helix | 246-251 | 6 | |
| α-helix | 254-262 | 9 | |
| α-helix | 264-279 | 16 | |
| α-helix | 286-288 | 3 | |
| β-strand | 294-298 | 5 | 1 |
| β-strand | 304 | 1 | 2 |
| α-helix | 306-308 | 3 | |
| α-helix | 311-313 | 3 | |
| β-strand | 317-327 | 11 | 2 |
| β-strand | 331-334 | 4 | 3 |
| β-strand | 336-337 | 2 | 4 |
| β-strand | 340-341 | 2 | 5 |
| β-strand | 347 | 1 | 5 |
| α-helix | 348-350 | 3 | |
| β-strand | 351-352 | 2 | 4 |
| β-strand | 373-374 | 2 | 5 |
| β-strand | 382-385 | 4 | 3 |
| β-strand | 386-391 | 6 | 2 |
| α-helix | 392-393 | 2 | |
| α-helix | 398 | 1 | |
| β-strand | 405-410 | 6 | 2 |
| β-strand | 423-433 | 11 | 2 |
| α-helix | 436-442 | 7 | |
| β-strand | 446 | 1 | 6 |
| β-strand | 448-458 | 11 | 2 |
| α-helix | 464-466 | 3 | |
| α-helix | 478-487 | 10 | |
| α-helix | 493-499 | 7 | |
| α-helix | 509-520 | 12 | |
| β-strand | 525-527 | 3 | 7 |
| β-strand | 531-533 | 3 | 7 |
| β-strand | 539-543 | 5 | 8 |
| α-helix | 549-559 | 11 | |
| β-strand | 563-567 | 5 | 8 |
| α-helix | 568-570 | 3 | |
| β-strand | 571 | 1 | 9 |
| β-strand | 579-582 | 4 | 10 |
| β-strand | 589-592 | 4 | 10 |
| α-helix | 595-598 | 4 | |
| β-strand | 602-607 | 6 | 8 |
| α-helix | 614-617 | 4 | |
| α-helix | 618-620 | 3 | |
| α-helix | 621-625 | 5 | |
| β-strand | 628-633 | 6 | 11 |
| β-strand | 636-641 | 6 | 11 |
| β-strand | 644-650 | 7 | 8 |
| α-helix | 653-655 | 3 | |
| α-helix | 663-665 | 3 | |
| α-helix | 671-674 | 4 | |
| β-strand | 679-683 | 5 | 8 |
| α-helix | 689-705 | 17 | |
| α-helix | 740-755 | 16 | |
| α-helix | 760-773 | 14 | |
| β-strand | 777 | 1 | 12 |
| α-helix | 783-798 | 16 | |
| α-helix | 807-809 | 3 | |
| α-helix | 810-823 | 14 | |
| β-strand | 828 | 1 | 12 |
| α-helix | 831-846 | 16 | |
| α-helix | 851-860 | 10 | |
Chains 3 and b: 30 helices, 30 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 19-34 | 16 | |
| α-helix | 36-53 | 18 | |
| β-strand | 95-99 | 5 | 21 |
| α-helix | 100-106 | 7 | |
| α-helix | 108-116 | 9 | |
| α-helix | 118-120 | 3 | |
| α-helix | 122-136 | 15 | |
| β-strand | 154-158 | 5 | 21 |
| β-strand | 159 | 1 | 22 |
| α-helix | 162-164 | 3 | |
| β-strand | 165-166 | 2 | 23 |
| α-helix | 173-175 | 3 | |
| β-strand | 178-189 | 12 | 23 |
| α-helix | 190-192 | 3 | |
| β-strand | 193-203 | 11 | 23 |
| β-strand | 209-213 | 5 | 23 |
| β-strand | 234 | 1 | 24 |
| β-strand | 240 | 1 | 24 |
| β-strand | 241-243 | 3 | 23 |
| β-strand | 249-259 | 11 | 23 |
| α-helix | 262-264 | 3 | |
| α-helix | 265-266 | 2 | |
| β-strand | 273-279 | 7 | 23 |
| α-helix | 280-282 | 3 | |
| β-strand | 291-301 | 11 | 23 |
| α-helix | 310-311 | 2 | |
| β-strand | 317 | 1 | 25 |
| β-strand | 318-328 | 11 | 23 |
| α-helix | 344-354 | 11 | |
| α-helix | 359-365 | 7 | |
| α-helix | 375-385 | 11 | |
| β-strand | 391-393 | 3 | 26 |
| β-strand | 397-399 | 3 | 26 |
| β-strand | 405-409 | 5 | 27 |
| α-helix | 416-425 | 10 | |
| β-strand | 429-433 | 5 | 27 |
| β-strand | 437 | 1 | 28 |
| α-helix | 439-442 | 4 | |
| β-strand | 444-448 | 5 | 29 |
| β-strand | 455-459 | 5 | 29 |
| α-helix | 461-464 | 4 | |
| β-strand | 468-473 | 6 | 27 |
| α-helix | 475-477 | 3 | |
| α-helix | 480-491 | 12 | |
| β-strand | 494-499 | 6 | 30 |
| β-strand | 502-507 | 6 | 30 |
| β-strand | 510-516 | 7 | 27 |
| α-helix | 529-532 | 4 | |
| α-helix | 537-540 | 4 | |
| β-strand | 545-549 | 5 | 27 |
| α-helix | 555-568 | 14 | |
| α-helix | 580-582 | 3 | |
| α-helix | 586-588 | 3 | |
| β-strand | 594 | 1 | 22 |
| α-helix | 653-666 | 14 | |
| β-strand | 670 | 1 | 31 |
| α-helix | 673-687 | 15 | |
| α-helix | 701-715 | 15 | |
| β-strand | 720 | 1 | 31 |
| α-helix | 723-738 | 16 | |
Chains 4 and c: 25 helices, 27 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 187-200 | 14 | |
| α-helix | 203-210 | 8 | |
| α-helix | 220-222 | 3 | |
| α-helix | 225-236 | 12 | |
| β-strand | 244 | 1 | 37 |
| α-helix | 245-250 | 6 | |
| α-helix | 255-261 | 7 | |
| α-helix | 265-284 | 20 | |
| α-helix | 294-298 | 5 | |
| β-strand | 306 | 1 | 37 |
| α-helix | 319-321 | 3 | |
| β-strand | 325-335 | 11 | 38 |
| β-strand | 339-349 | 11 | 38 |
| β-strand | 355-359 | 5 | 38 |
| β-strand | 361 | 1 | 39 |
| β-strand | 364 | 1 | 39 |
| β-strand | 370 | 1 | 40 |
| β-strand | 379 | 1 | 40 |
| β-strand | 382-384 | 3 | 38 |
| α-helix | 386-388 | 3 | |
| β-strand | 390-400 | 11 | 38 |
| β-strand | 414-420 | 7 | 38 |
| α-helix | 421-423 | 3 | |
| β-strand | 432-442 | 11 | 38 |
| β-strand | 445 | 1 | 41 |
| β-strand | 453-454 | 2 | 41 |
| β-strand | 458-468 | 11 | 38 |
| α-helix | 503-513 | 11 | |
| α-helix | 518-525 | 8 | |
| α-helix | 534-545 | 12 | |
| β-strand | 550-552 | 3 | 42 |
| β-strand | 556-558 | 3 | 42 |
| β-strand | 564-568 | 5 | 43 |
| α-helix | 574-584 | 11 | |
| β-strand | 590-592 | 3 | 43 |
| β-strand | 604-607 | 4 | 44 |
| β-strand | 614-617 | 4 | 44 |
| α-helix | 622-624 | 3 | |
| β-strand | 628-632 | 5 | 43 |
| α-helix | 634-636 | 3 | |
| α-helix | 639-648 | 10 | |
| β-strand | 653-658 | 6 | 45 |
| β-strand | 661-666 | 6 | 45 |
| β-strand | 670-674 | 5 | 43 |
| α-helix | 688-692 | 5 | |
| α-helix | 696-700 | 5 | |
| β-strand | 704-708 | 5 | 43 |
| α-helix | 714-728 | 15 | |
| α-helix | 746-756 | 11 | |
| α-helix | 764-777 | 14 | |
| α-helix | 795-810 | 16 | |
| α-helix | 819-834 | 16 | |
Chains 5 and d: 33 helices, 30 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 24-37 | 14 | |
| β-strand | 40 | 1 | 46 |
| β-strand | 43 | 1 | 46 |
| α-helix | 45-55 | 11 | |
| β-strand | 61-64 | 4 | 47 |
| α-helix | 65-71 | 7 | |
| α-helix | 73-81 | 9 | |
| α-helix | 83-102 | 20 | |
| β-strand | 137-140 | 4 | 47 |
| α-helix | 144-147 | 4 | |
| α-helix | 148-150 | 3 | |
| α-helix | 153-155 | 3 | |
| β-strand | 158-165 | 8 | 25 |
| β-strand | 168-169 | 2 | 25 |
| β-strand | 173-182 | 10 | 25 |
| β-strand | 189-193 | 5 | 25 |
| β-strand | 242-259 | 18 | 25 |
| α-helix | 262-264 | 3 | |
| β-strand | 273-279 | 7 | 25 |
| α-helix | 280-282 | 3 | |
| β-strand | 291-301 | 11 | 25 |
| β-strand | 323 | 1 | 3 |
| β-strand | 325-335 | 11 | 25 |
| α-helix | 351-361 | 11 | |
| α-helix | 366-372 | 7 | |
| α-helix | 382-393 | 12 | |
| β-strand | 398-399 | 2 | 48 |
| β-strand | 405-406 | 2 | 48 |
| β-strand | 412-416 | 5 | 49 |
| α-helix | 423-432 | 10 | |
| β-strand | 436-440 | 5 | 49 |
| β-strand | 444 | 1 | 11 |
| α-helix | 446-449 | 4 | |
| β-strand | 451-455 | 5 | 50 |
| β-strand | 462-466 | 5 | 50 |
| α-helix | 468-471 | 4 | |
| β-strand | 476-480 | 5 | 49 |
| α-helix | 482-484 | 3 | |
| α-helix | 487-490 | 4 | |
| α-helix | 491-493 | 3 | |
| α-helix | 494-498 | 5 | |
| β-strand | 501-506 | 6 | 28 |
| β-strand | 509-514 | 6 | 28 |
| β-strand | 518-523 | 6 | 49 |
| α-helix | 536-538 | 3 | |
| α-helix | 544-547 | 4 | |
| β-strand | 552-556 | 5 | 49 |
| α-helix | 562-576 | 15 | |
| α-helix | 581-590 | 10 | |
| α-helix | 596-609 | 14 | |
| β-strand | 614 | 1 | 51 |
| α-helix | 616-640 | 25 | |
| α-helix | 650-666 | 17 | |
| β-strand | 672 | 1 | 51 |
| α-helix | 674-684 | 11 | |
| α-helix | 685-689 | 5 | |
| α-helix | 690-692 | 3 | |
| α-helix | 707-721 | 15 | |
| β-strand | 727-729 | 3 | 52 |
| α-helix | 730-739 | 10 | |
| α-helix | 748-756 | 9 | |
| β-strand | 762-764 | 3 | 52 |
| β-strand | 769-771 | 3 | 52 |
Chains 6 and e: 31 helices, 27 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 107-121 | 15 | |
| α-helix | 135-146 | 12 | |
| β-strand | 150-154 | 5 | 53 |
| α-helix | 155-158 | 4 | |
| α-helix | 162-164 | 3 | |
| α-helix | 165-172 | 8 | |
| α-helix | 174-192 | 19 | |
| α-helix | 194-197 | 4 | |
| β-strand | 264-268 | 5 | 53 |
| β-strand | 274 | 1 | 6 |
| α-helix | 276-278 | 3 | |
| α-helix | 281-283 | 3 | |
| β-strand | 287-297 | 11 | 6 |
| β-strand | 301-311 | 11 | 6 |
| β-strand | 317-322 | 6 | 6 |
| β-strand | 344-346 | 3 | 6 |
| β-strand | 352-362 | 11 | 6 |
| α-helix | 373-375 | 3 | |
| β-strand | 376-382 | 7 | 6 |
| α-helix | 383-385 | 3 | |
| β-strand | 394-405 | 12 | 6 |
| α-helix | 408-410 | 3 | |
| α-helix | 425-427 | 3 | |
| β-strand | 437 | 1 | 54 |
| β-strand | 438 | 1 | 39 |
| β-strand | 448 | 1 | 54 |
| β-strand | 450-461 | 12 | 6 |
| α-helix | 501-507 | 7 | |
| α-helix | 510-521 | 12 | |
| α-helix | 525-532 | 8 | |
| α-helix | 541-552 | 12 | |
| β-strand | 557-558 | 2 | 55 |
| α-helix | 563 | 1 | |
| β-strand | 564-565 | 2 | 55 |
| α-helix | 566 | 1 | |
| β-strand | 571-575 | 5 | 56 |
| α-helix | 581-591 | 11 | |
| β-strand | 596-599 | 4 | 56 |
| β-strand | 610-614 | 5 | 57 |
| β-strand | 621-625 | 5 | 57 |
| α-helix | 629-631 | 3 | |
| β-strand | 635-639 | 5 | 56 |
| α-helix | 641-643 | 3 | |
| α-helix | 646-649 | 4 | |
| α-helix | 652-655 | 4 | |
| β-strand | 660-664 | 5 | 9 |
| β-strand | 669-673 | 5 | 9 |
| β-strand | 677-682 | 6 | 56 |
| α-helix | 695-698 | 4 | |
| α-helix | 703-706 | 4 | |
| β-strand | 711-715 | 5 | 56 |
| α-helix | 721-733 | 13 | |
| α-helix | 748-758 | 11 | |
| β-strand | 764-765 | 2 | 58 |
| α-helix | 767-784 | 18 | |
| α-helix | 797-813 | 17 | |
| β-strand | 818-819 | 2 | 58 |
| α-helix | 821-833 | 13 | |
Chains 7 and f: 32 helices, 22 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 7-8 | 2 | 23 |
| α-helix | 14-27 | 14 | |
| α-helix | 62-71 | 10 | |
| β-strand | 77-81 | 5 | 59 |
| α-helix | 82-95 | 14 | |
| α-helix | 102-108 | 7 | |
| α-helix | 111-123 | 13 | |
| α-helix | 125-128 | 4 | |
| α-helix | 138-152 | 15 | |
| α-helix | 194-197 | 4 | |
| β-strand | 201-205 | 5 | 59 |
| α-helix | 209-215 | 7 | |
| β-strand | 224-225 | 2 | 60 |
| α-helix | 232-234 | 3 | |
| β-strand | 238-248 | 11 | 60 |
| α-helix | 249-251 | 3 | |
| β-strand | 252-261 | 10 | 60 |
| β-strand | 269-272 | 4 | 60 |
| β-strand | 277-278 | 2 | 41 |
| α-helix | 288-290 | 3 | |
| β-strand | 299-316 | 18 | 60 |
| α-helix | 319-321 | 3 | |
| α-helix | 323 | 1 | |
| β-strand | 330-336 | 7 | 60 |
| β-strand | 348-358 | 11 | 60 |
| α-helix | 365-368 | 4 | |
| β-strand | 371 | 1 | 23 |
| β-strand | 373-383 | 11 | 60 |
| α-helix | 395-407 | 13 | |
| α-helix | 410-417 | 8 | |
| α-helix | 426-436 | 11 | |
| β-strand | 443 | 1 | 61 |
| α-helix | 445-447 | 3 | |
| β-strand | 449 | 1 | 61 |
| β-strand | 455-460 | 6 | 62 |
| α-helix | 466-476 | 11 | |
| β-strand | 480-483 | 4 | 62 |
| α-helix | 512-515 | 4 | |
| β-strand | 520-523 | 4 | 62 |
| α-helix | 531-543 | 13 | |
| β-strand | 545-547 | 3 | 63 |
| α-helix | 548-549 | 2 | |
| β-strand | 556-558 | 3 | 63 |
| β-strand | 562-566 | 5 | 62 |
| α-helix | 580-583 | 4 | |
| α-helix | 588-593 | 6 | |
| β-strand | 596-600 | 5 | 62 |
| α-helix | 606-621 | 16 | |
| α-helix | 625-627 | 3 | |
| α-helix | 635-647 | 13 | |
| α-helix | 654-674 | 21 | |
| α-helix | 686-703 | 18 | |
| α-helix | 708-709 | 2 | |
| α-helix | 710-725 | 16 | |
Chains C and J: 10 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-11 | 6 | |
| β-strand | 15-20 | 6 | 64 |
| β-strand | 24 | 1 | 65 |
| α-helix | 37 | 1 | |
| β-strand | 38 | 1 | 65 |
| β-strand | 43-47 | 5 | 64 |
| α-helix | 48-54 | 7 | |
| β-strand | 72-74 | 3 | 64 |
| α-helix | 75-77 | 3 | |
| α-helix | 82-90 | 9 | |
| α-helix | 97-100 | 4 | |
| α-helix | 104-114 | 11 | |
| α-helix | 118-140 | 23 | |
| α-helix | 160-166 | 7 | |
| α-helix | 171-191 | 21 | |
Chains D and K: 13 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 56-72 | 17 | |
| α-helix | 77-79 | 3 | |
| α-helix | 81-111 | 31 | |
| α-helix | 114-116 | 3 | |
| α-helix | 118 | 1 | |
| α-helix | 123-153 | 31 | |
| α-helix | 155-163 | 9 | |
| α-helix | 171-174 | 4 | |
| α-helix | 177-197 | 21 | |
| α-helix | 199-201 | 3 | |
| α-helix | 204-206 | 3 | |
| β-strand | 227-232 | 6 | 66 |
| β-strand | 247-249 | 3 | 67 |
| β-strand | 252-260 | 9 | 67 |
| β-strand | 265-270 | 6 | 67 |
| β-strand | 274-278 | 5 | 66 |
| α-helix | 280-282 | 3 | |
| α-helix | 283-287 | 5 | |
| β-strand | 291-293 | 3 | 66 |
5 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| DNA replication licensing factor MCM2 | 2, a | protein | 868 | Saccharomyces cerevisiae | A0A6A5Q1S9 |
| DNA replication licensing factor MCM3 | 3, b | protein | 1006 | Saccharomyces cerevisiae | P24279 (AlphaFold model) |
| DNA replication licensing factor MCM4 | 4, c | protein | 933 | Saccharomyces cerevisiae | P30665 (AlphaFold model) |
| DNA helicase | 5, d | protein | 775 | Saccharomyces cerevisiae | A0A6A5PUY8 |
| DNA replication licensing factor MCM6 | 6, e | protein | 1017 | Saccharomyces cerevisiae | P53091 |
| DNA replication licensing factor MCM7 | 7, f | protein | 845 | Saccharomyces cerevisiae | A0A6A5Q4N0 |
| DNA (53-mer) | A | DNA | 53 | DNA molecule | |
| DNA (53-mer) | B | DNA | 53 | DNA molecule | |
| DNA replication complex GINS protein PSF3 | C, J | protein | 229 | Saccharomyces cerevisiae | Q12146 |
| DNA replication complex GINS protein SLD5 | D, K | protein | 294 | Saccharomyces cerevisiae | Q03406 |
| Cell division control protein 45 | E, L | protein | 657 | Saccharomyces cerevisiae | Q08032 |
| DNA polymerase epsilon subunit B | F, M | protein | 689 | Saccharomyces cerevisiae | P24482 |
3 more molecules are not listed.
Sequence of entity 1 (2, a), FASTA
>7Z13_1 DNA replication licensing factor MCM2 (chains 2, a)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH
Sequence of entity 2 (3, b), FASTA
>7Z13_2 DNA replication licensing factor MCM3 (chains 3, b)
MKRRWKKNFIAVSAANRFKKISSSGALENLYFQGEMEGSTGFDGDATTFFAPDAVFGDRV
RRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQDDADERDLLGDDDGDDLEKEKKAAS
STSLNILPHRIIISLDDLREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHPNAS
AVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHYAAK
TGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITVQEMPEMA
PAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQSNSNTLIGFKTLILGNT
VYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIFDILSQSLAPSIYGHDHIKKAILLML
MGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGLTAA
VTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGIHTT
LNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSISEHV
LRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINPEEHSNSGAGVENEGEDDEDHVFEKF
NPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTD
LRNDDNTKKSPITARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALLGEDIGND
IDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSGSPIKSTPRRSTASSVNATPSSARRI
LRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQLGLRVSPRRREHLHAPEEGSSGPLTE
VGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTISTGRLSLISGIIARLMQTEIFEEESYP
VASLFERINEELPEEEKFSAQEYLAGLKIMSDRNNLMVADDKVWRV
Sequence of entity 3 (4, c), FASTA
>7Z13_3 DNA replication licensing factor MCM4 (chains 4, c)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV
Sequence of entity 4 (5, d), FASTA
>7Z13_4 DNA helicase (chains 5, d)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV
Sequence of entity 5 (6, e), FASTA
>7Z13_5 DNA replication licensing factor MCM6 (chains 6, e)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS
Sequence of entity 6 (7, f), FASTA
>7Z13_6 DNA replication licensing factor MCM7 (chains 7, f)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA
Sequence of entity 7 (A), FASTA
>7Z13_7 DNA (53-MER) (chains A)
TTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAA
Sequence of entity 8 (B), FASTA
>7Z13_8 DNA (53-MER) (chains B)
TTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAA
Sequence of entity 9 (C, J), FASTA
>7Z13_9 DNA replication complex GINS protein PSF3 (chains C, J)
WSHPQFEKGGGSGGGSGGGSWSHPQFEKENLYFQSMGYYDIDDVLADGTEFPCKFQYDIP
GLGYLENNPGRPITKNTKLSLPLWLARILAIVGGDEALVDEEPVPFVELLPPDMFSTKVM
NAIKTDPVALDLHSINSHFFSLAIKWIMLFSEKELANVVSELLLQRAQELNHHASSLSID
LNADSTGKNSANTNIATSTFLLKLEEMEKEIYKKSHESYKDTKRWMFKK
Sequence of entity 10 (D, K), FASTA
>7Z13_10 DNA replication complex GINS protein SLD5 (chains D, K)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI
Sequence of entity 11 (E, L), FASTA
>7Z13_11 Cell division control protein 45 (chains E, L)
MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVP
IFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDI
YVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDEND
NNGGDDEATDADEVTDEDYKDDDGDYKDDDETISNKRGNSSIGPNDLSKRKQRKKQIHEY
EGVLEEYYSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYP
LLQDEVKRLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNE
NGKKRLHKMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTL
GYRGSISASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRW
VSNFWLSWDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDG
PDLDLYRNPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLD
TIHTKKPILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL
Sequence of entity 12 (F, M), FASTA
>7Z13_12 DNA polymerase epsilon subunit B (chains F, M)
MFGSGNVLPVKIQPPLLRPLAYRVLSRKYGLSIKSDGLSALAEFVGTNIGANWRQGPATI
KFLEQFAAVWKQQERGLFIDQSGVKEVIQEMKEREKVEWSHEHPIQHEENILGRTDDDEN
NSDDEMPIAADSSLQNVSLSSPMRQPTERDEYKQPFKPESSKALDWRDYFKVINASQQQR
FSYNPHKMQFIFVPNKKQNGLGGIAGFLPDIEDKVQMFLTRYYLTNDRVMRNENFQNSDM
FNPLSSMVSLQNELSNTNRQQQSSSMSITPIKNLLGRDAQNFLLLGLLNKNFKGNWSLED
PSGSVEIDISQTIPTQGHYYVPGCMVLVEGIYYSVGNKFHVTSMTLPPGERREITLETIG
NLDLLGIHGISNNNFIARLDKDLKIRLHLLEKELTDHKFVILGANLFLDDLKIMTALSKI
LQKLNDDPPTLLIWQGSFTSVPVFASMSSRNISSSTQFKNNFDALATLLSRFDNLTENTT
MIFIPGPNDLWGSMVSLGASGTLPQDPIPSAFTKKINKVCKNVVWSSNPTRIAYLSQEIV
IFRDDLSGRFKRHRLEFPFNESEDVYTENDNMMSKDTDIVPIDELVKEPDQLPQKVQETR
KLVKTILDQGHLSPFLDSLRPISWDLDHTLTLCPIPSTMVLCDTTSAQFDLTYNGCKVIN
PGSFIHNRRARYMEYVPSSKKTIQEEIYI
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ATP | Adenosine-5'-triphosphate | C10 H16 N5 O13 P3 | 8 |
| ZN | Zinc ion | Zn | 14 |
| MG | Magnesium ion | Mg | 6 |
| ADP | Adenosine-5'-diphosphate | C10 H15 N5 O10 P2 | 4 |
Primary citation
Mechanism of replication origin melting nucleated by CMG helicase assembly. Lewis, J.S., Gross, M.H., Sousa, J. et al. Nature (2022) 606:1007-1014. DOI 10.1038/s41586-022-04829-4 · PubMed
Other PDB entries of the same protein (UniProt A0A6A5Q1S9), best resolution first:
- 7QHS 3.3 Å, S. cerevisiae CMGE nucleating origin DNA melting
- 8W7S 7.39 Å, Yeast replisome in state IV
Browse structure collections
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