8BO2: Outer membrane protein assembly factor BamA

BAM-EspP complex structure with BamA-S425C/EspP-S1299C mutations in nanodisc. Determined by electron microscopy at 3.1 Å resolution. Released 26 Apr 2023.

Method: Electron microscopy
Resolution: 3.1 Å
Organism: Escherichia coli K-12
Chains: 6
Atoms: 14,037
Mol. weight: 247.21 kDa
Released: 26 Apr 2023

Explore 8BO2 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8BO2 contains 43 α-helices and 103 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 22 helices, 47 β-strands

Element	Residues	Length	Sheet
β-strand	25	1	1
β-strand	28-32	5	2
α-helix	39-45	7
β-strand	53	1	1
α-helix	56-68	13
β-strand	72-80	9	2
β-strand	83-90	8	2
α-helix	91-92	2
β-strand	93-100	8	3
α-helix	107-116	10
α-helix	127-144	18
β-strand	150-158	9	3
β-strand	162-170	9	3
β-strand	176-181	6	4
α-helix	190-196	7
α-helix	216-233	18
β-strand	239-247	9	4
β-strand	253-261	9	4
β-strand	267-276	10	5
α-helix	281-285	5
α-helix	294	1
β-strand	295	1	5
α-helix	296	1
α-helix	298-314	17
β-strand	317	1	6
β-strand	321-329	9	5
β-strand	334-342	9	5
β-strand	346	1	6
β-strand	348-355	8	7
α-helix	362-367	6
α-helix	372	1
β-strand	376	1	7
α-helix	379-392	14
β-strand	395-404	10	7
α-helix	405	1
β-strand	411-420	10	7
β-strand	426-427	2	8
β-strand	430-433	4	8
β-strand	437-446	10	8
α-helix	449-451	3
β-strand	455-462	8	8
β-strand	466-475	10	8
β-strand	484-494	11	8
β-strand	505-518	14	8
β-strand	523-537	15	8
β-strand	542	1	9
α-helix	546-551	6
α-helix	556-557	2
β-strand	564-579	16	8
β-strand	590-600	11	8
α-helix	601	1
β-strand	608-620	13	8
β-strand	628-640	13	8
α-helix	648-650	3
β-strand	652	1	10
β-strand	654	1	11
β-strand	663	1	12
β-strand	668	1	11
α-helix	670	1
β-strand	671-674	4	9
α-helix	675-676	2
β-strand	700-705	6	9
β-strand	708	1	10
β-strand	710-720	11	8
β-strand	733-745	13	8
β-strand	767-777	11	8
β-strand	782-792	11	8
β-strand	799	1	12
β-strand	802-806	5	8

Chain B: 4 helices, 35 β-strands

Element	Residues	Length	Sheet
α-helix	31-34	4
β-strand	39	1	13
α-helix	43-44	2
β-strand	49-51	3	14
α-helix	52	1
β-strand	66-68	3	15
β-strand	71-76	6	15
β-strand	80-84	5	15
β-strand	95-96	2	15
β-strand	115-118	4	16
β-strand	121-124	4	16
β-strand	130-135	6	16
β-strand	143-146	4	16
β-strand	151	1	17
β-strand	156-158	3	17
β-strand	161-166	6	17
β-strand	170-175	6	17
β-strand	184-186	3	17
β-strand	193	1	4
β-strand	201-203	3	18
β-strand	206-209	4	18
β-strand	215-219	5	18
β-strand	228-231	4	18
β-strand	253-254	2	19
β-strand	257-261	5	19
β-strand	267-271	5	19
β-strand	279-281	3	19
α-helix	288-289	2
β-strand	290-292	3	20
β-strand	295-299	5	20
β-strand	305-309	5	20
β-strand	315-319	5	20
β-strand	331-333	3	13
β-strand	336-340	5	13
β-strand	346-349	4	13
β-strand	356-360	5	13
β-strand	372-374	3	14
β-strand	377-380	4	14
β-strand	386-390	5	14

Chain C: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	49-51	3
α-helix	72-74	3

Chain D: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	32-44	13
α-helix	53-61	9
α-helix	69-81	13
α-helix	85-98	14
α-helix	105-119	15
α-helix	131-133	3
α-helix	138-153	16
α-helix	160-188	29
α-helix	191-204	14
α-helix	209-223	15
α-helix	228-238	11

Chain E: 2 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	32-35	4	21
α-helix	40-44	5
α-helix	52-59	8
β-strand	72-80	9	21
β-strand	87-94	8	21
β-strand	103-107	5	21

Chain P: 2 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	1001-1023	23
α-helix	1026-1034	9
β-strand	1039-1052	14	22
β-strand	1056-1073	18	22
β-strand	1076-1093	18	22
β-strand	1096-1112	17	22
β-strand	1117-1134	18	22
β-strand	1140-1157	18	22
β-strand	1159-1162	4	22
β-strand	1165-1178	14	22
β-strand	1182-1187	6	23
β-strand	1190-1195	6	23
β-strand	1202-1215	14	22
β-strand	1220-1232	13	22
β-strand	1238-1243	6	23
β-strand	1246-1251	6	23
β-strand	1257-1269	13	22
β-strand	1272-1282	11	22
β-strand	1286-1297	12	22

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Outer membrane protein assembly factor BamA	A	protein	787	Escherichia coli K-12	B1XD46 (AlphaFold model)
Outer membrane protein assembly factor BamB	B	protein	392	Escherichia coli K-12	P77774 (AlphaFold model)
Outer membrane protein assembly factor BamC	C	protein	344	Escherichia coli K-12	P0A903 (AlphaFold model)
Outer membrane protein assembly factor BamD	D	protein	245	Escherichia coli K-12	P0AC02 (AlphaFold model)
Outer membrane protein assembly factor BamE	E	protein	123	Escherichia coli K-12	P0A937
Serine protease EspP	P	protein	353	Escherichia coli K-12	Q7BSW5

Sequence of entity 1 (A), FASTA

>8BO2_1 Outer membrane protein assembly factor BamA (chains A)
FVVKDIHFEGLQRVAVGAALLSMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDT
LLVQVKERPTIASITFSGNKSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYS
VGKYSASVKAVVTPLPRNRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEV
PWWNVVGDRKYQKQKLAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEG
DQYKLSGVEVSGNLAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQ
SMPEINDADKTVKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVD
QGKERLNRLGFFETVDTDTQRVPGSPDQVDVVYKVKERNTGCFNFGIGYGTESGVSFQAG
VQQDNWLGTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSD
YTNKSYGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDN
SFKTDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDD
DHKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNYD
PDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWDMGT
VWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDKAEQFQ
FNIGKTW

Sequence of entity 2 (B), FASTA

>8BO2_2 Outer membrane protein assembly factor BamB (chains B)
MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTPTTAWSTSVGSGIGNFY
SNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDGWFSKEPALLSGGVTVSGG
HVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDGLVLIHTSNGQLQALNEADGA
VKWTVNLDMPSLSLRGESAPTTAFGAAVVGGDNGRVSAVLMEQGQMIWQQRISQATGSTE
IDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQIMWKRELGSVNDFIVDGNRIYLVD
QNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYNGNLVVGDSEGYLHWINVEDGRFVAQQ
KVDSSGFQTEPVAADGKLLIQAKDGTVYSITR

Sequence of entity 3 (C), FASTA

>8BO2_3 Outer membrane protein assembly factor BamC (chains C)
MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEAAPLAELHAPAGMILPV
TSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGDTASLLVENGRGNTLWPQV
VSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGRYQISVKPQGYQQAVTVKLLN
LEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAANAAQNRASTTMDVQSAADDTGLP
MLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNMAVTYKPLSDSDWQELGASDPGLAS
GDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDALVAVFQAAFSK

Sequence of entity 4 (D), FASTA

>8BO2_4 Outer membrane protein assembly factor BamD (chains D)
MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDN
RYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALD
DSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYTTDATKRLVFLKDRLAKYEYS
VAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA
NSSNT

Sequence of entity 5 (E), FASTA

>8BO2_5 Outer membrane protein assembly factor BamE (chains E)
MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVSKIRVGMTQQQVAYALG
TPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLTNIDNKPALSGNGGHHHHH
HHH

Sequence of entity 6 (P), FASTA

>8BO2_6 Serine protease EspP (chains P)
NIELVSAPKDTNENVFKASKQTIGFSDVTPVITTRETDDKITWSLTGYNTVANKEATRNA
AALFSVDYKAFLNEVNNLNKRMGDLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGV
DKKHELDGLDLFTGFTVTHTDSSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVH
HDNEYTATFAGLGTRDYSTHSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKD
QGMHLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGE
KRIKGEKDSRMLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYCF

Primary citation

Structural basis of BAM-mediated outer membrane beta-barrel protein assembly. Shen, C., Chang, S., Luo, Q. et al. Nature (2023) 617:185-193. DOI 10.1038/s41586-023-05988-8 · PubMed

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