Cryo-EM structure of CDK2-CyclinA in complex with p27 from the SCFSKP2 E3 ligase Complex. Determined by electron microscopy at 3.5 Å resolution. Released 28 Jun 2023.
Explore 8BZO in 3D Show helices and sheets RCSB PDB PDBe
8BZO contains 33 α-helices and 12 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 18-22 | 5 | 1 |
| β-strand | 30-36 | 7 | 1 |
| α-helix | 46-54 | 9 | |
| β-strand | 63 | 1 | 2 |
| β-strand | 66-71 | 6 | 1 |
| β-strand | 75-81 | 7 | 1 |
| β-strand | 85-86 | 2 | 2 |
| α-helix | 87-93 | 7 | |
| α-helix | 101-120 | 20 | |
| β-strand | 133-135 | 3 | 2 |
| β-strand | 141-143 | 3 | 2 |
| α-helix | 166-168 | 3 | |
| α-helix | 171-175 | 5 | |
| α-helix | 183-198 | 16 | |
| α-helix | 208-219 | 12 | |
| β-strand | 245 | 1 | 3 |
| α-helix | 248-251 | 4 | |
| α-helix | 259-266 | 8 | |
| α-helix | 275-276 | 2 | |
| α-helix | 277-280 | 4 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 179-189 | 11 | |
| α-helix | 195-196 | 2 | |
| α-helix | 208-223 | 16 | |
| α-helix | 229-242 | 14 | |
| α-helix | 253-268 | 16 | |
| α-helix | 272-274 | 3 | |
| α-helix | 275-281 | 7 | |
| α-helix | 288-291 | 4 | |
| α-helix | 295-301 | 7 | |
| α-helix | 311-319 | 9 | |
| α-helix | 332-342 | 11 | |
| α-helix | 352-367 | 16 | |
| α-helix | 375-377 | 3 | |
| α-helix | 388-400 | 13 | |
| α-helix | 401-403 | 3 | |
| α-helix | 409-414 | 6 | |
| α-helix | 416-418 | 3 | |
| α-helix | 421-423 | 3 | |
| α-helix | 425-427 | 3 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 38-51 | 14 | |
| β-strand | 63 | 1 | 4 |
| β-strand | 68 | 1 | 4 |
| β-strand | 75-79 | 5 | 1 |
| α-helix | 86-89 | 4 | |
| α-helix | 90-92 | 3 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Cyclin-dependent kinase 2 | A | protein | 297 | Homo sapiens | P24941 (AlphaFold model) |
| Cyclin-A2 | B | protein | 432 | Homo sapiens | P20248 (AlphaFold model) |
| Cyclin-dependent kinase inhibitor 1B | C | protein | 158 | Homo sapiens | P46527 (AlphaFold model) |
>8BZO_1 Cyclin-dependent kinase 2 (chains A) MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNH PNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHS HRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYY STAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSF PKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLR
>8BZO_2 Cyclin-A2 (chains B) MLGNSAPGPATREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRG LAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKI EREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHE DIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYID RFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLK VLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAF HLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYHG VSLLNPPETLNL
>8BZO_3 Cyclin-dependent kinase inhibitor 1B (chains C) MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKW NFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDGSGSRPAAPLIG APANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATD
Cryo-EM structure of SKP1-SKP2-CKS1 in complex with CDK2-cyclin A-p27KIP1. Rowland, R.J., Heath, R., Maskell, D. et al. Sci Rep (2023) 13:10718-10718. DOI 10.1038/s41598-023-37609-9 · PubMed
Other PDB entries of the same protein (UniProt P24941 (AlphaFold model), which also has an AlphaFold model), best resolution first:
MolViewer shows 8BZO directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.