8CAZ: Empty 30S head

Name: PDB 8CAZ: empty 30S head
Published: 2023-07-26
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: ribosome

empty 30S head. Determined by electron microscopy at 2.11 Å resolution. Released 26 Jul 2023.

Method: Electron microscopy
Resolution: 2.11 Å
Organism: Escherichia coli BW25113
Chains: 10
Atoms: 19,193
Mol. weight: 642.93 kDa
Ligands: MG
Released: 26 Jul 2023

Explore 8CAZ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8CAZ contains 58 α-helices and 41 β-strands across 9 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 4: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	56-63	8

Chain B: 12 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	8-12	5
β-strand	16-19	4	1
α-helix	20-22	3
α-helix	25-27	3
β-strand	31-34	4	1
β-strand	38-41	4	1
α-helix	43-62	20
β-strand	67-69	3	2
α-helix	81-85	5
β-strand	90-91	2	2
α-helix	96-97	2
α-helix	104-123	20
α-helix	125-127	3
α-helix	135-148	14
α-helix	149-151	3
β-strand	160-164	5	2
α-helix	170-179	10
β-strand	183-187	5	2
β-strand	198-201	4	2
α-helix	207-226	20

Chain C: 9 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
β-strand	12	1	3
β-strand	16	1	3
β-strand	20-21	2	4
α-helix	26-46	21
β-strand	52-58	7	4
β-strand	65-70	6	4
α-helix	73-77	5
α-helix	79-81	3
α-helix	82-95	14
β-strand	100-105	6	4
α-helix	109-111	3
α-helix	113-125	13
α-helix	130-143	14
β-strand	148-155	8	5
α-helix	157-159	3
β-strand	164-171	8	5
β-strand	180-190	11	5
β-strand	195-206	12	5

Chain G: 8 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	13-15	3
α-helix	21-30	10
α-helix	36-50	15
α-helix	58-67	10
β-strand	73-79	7	6
β-strand	84-90	7	6
α-helix	93-110	18
α-helix	116-129	14
α-helix	133-147	15
α-helix	149-151	3

Chain I: 7 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	6-12	7	7
β-strand	15-23	9	7
β-strand	28-30	3	7
β-strand	33-34	2	7
α-helix	35-38	4
α-helix	42-55	14
β-strand	61-68	8	7
α-helix	72-90	19
α-helix	92-94	3
α-helix	95-101	7
α-helix	111-114	4
α-helix	124-127	4

Chain J: 3 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	6-12	7	8
α-helix	15-31	17
β-strand	35-52	18	8
β-strand	62-77	16	8
α-helix	81-87	7
α-helix	92-93	2
β-strand	96-101	6	8

Chain M: 7 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	3-4	2	9
β-strand	7-8	2	9
β-strand	14	1	10
α-helix	15-18	4
α-helix	19-21	3
α-helix	27-37	11
β-strand	44	1	10
α-helix	50-60	11
α-helix	66-83	18
α-helix	86-93	8
α-helix	107-110	4

Chain N: 5 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	4-32	29
α-helix	38-50	13
α-helix	53-55	3
α-helix	57-59	3
β-strand	73-74	2	11
β-strand	79-80	2	11
α-helix	81-89	9
β-strand	96-98	3	8

1 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Large ribosomal subunit protein bL31A	4	protein	70	Escherichia coli BW25113	P0A7M9 (AlphaFold model)
16S rRNA	A	RNA	1540	Escherichia coli BW25113
30S ribosomal protein S2	B	protein	241	Escherichia coli BW25113	P0A7V0 (AlphaFold model)
Small ribosomal subunit protein uS3	C	protein	233	Escherichia coli BW25113	P0A7V3 (AlphaFold model)
30S ribosomal protein S7	G	protein	179	Escherichia coli BW25113	P02359 (AlphaFold model)
Small ribosomal subunit protein uS9	I	protein	130	Escherichia coli BW25113	P0A7X3
Small ribosomal subunit protein uS10	J	protein	103	Escherichia coli BW25113	P0A7R5
Small ribosomal subunit protein uS13	M	protein	118	Escherichia coli BW25113	P0A7S9
Small ribosomal subunit protein uS14	N	protein	101	Escherichia coli BW25113	P0AG59
Small ribosomal subunit protein uS19	S	protein	92	Escherichia coli BW25113	P0A7U3

Sequence of entity 1 (4), FASTA

>8CAZ_1 Large ribosomal subunit protein bL31A (chains 4)
MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFFTGKQRDVATGGRVDRF
NKRFNIPGSK

Sequence of entity 2 (A), FASTA

>8CAZ_2 16S rRNA (chains A)
AAAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAA
GUCGAACGGUAACAGGAAGAAGCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAA
UGUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAAACGGUAGCUAAUACCGCAU
AACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUG
GGAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGA
GGAUGACCAGCCACACUGGAACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGG
GGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGCCGCGUGUAUGAAGAAGGCCU
UCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUU
GACGUUACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAGCAGCCGCGGUAAUACGGAG
GGUGCAAGCGUUAAUCGGAAUUACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCA
GAUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGAUACUGGCAAGCUUGAGUCUC
GUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACC
GGUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCA
AACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCC
CUUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACCGCCUGGGGAGUACGGCCGCA
AGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAU
UCGAUGCAACGCGAAGAACCUUACCUGGUCUUGACAUCCACGGAAGUUUUCAGAGAUGAG
AAUGUGCCUUCGGGAACCGUGAGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGA
AAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCCUUUGUUGCCAGCGGUCCGGC
CGGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUC
AUCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCG
ACCUCGCGAGAGCAAGCGGACCUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAAC
UCGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCAGAAUGCCACGGUGAAUACGU
UCCCGGGCCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGU
AGCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGAAGUCGUAA
CAAGGUAACCGUAGGGGAACCUGCGGUUGGAUCACCUCCU

Sequence of entity 3 (B), FASTA

>8CAZ_3 30S ribosomal protein S2 (chains B)
MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEALAELNKI
ASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLTNWKTVRQSIKRLKDLETQ
SQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDALFVIDADHEHIAIKEANNLG
IPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVAATVREGRSQDLASQAEESFVEA
E

Sequence of entity 4 (C), FASTA

>8CAZ_4 Small ribosomal subunit protein uS3 (chains C)
MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLTKELAKASVSRIVIERP
AKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQINIAEVRKPELDAKLVADSI
TSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGAEIARTEWYREGRVPLHTLRA
DIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPEKPAAQPKKQQRKGRK

Sequence of entity 5 (G), FASTA

>8CAZ_5 30S ribosomal protein S7 (chains G)
MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELE
AFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRL
ANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRWLSLRSFSHQAGASSKQPALGYLN

Sequence of entity 6 (I), FASTA

>8CAZ_6 Small ribosomal subunit protein uS9 (chains I)
MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETARMVVRQPLELVDMVEK
LDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAGFVTRDARQVERKKVGLRK
ARRRPQFSKR

Sequence of entity 7 (J), FASTA

>8CAZ_7 Small ribosomal subunit protein uS10 (chains J)
MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTRKERFTVLISPHVNKD
ARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISLG

Sequence of entity 8 (M), FASTA

>8CAZ_8 Small ribosomal subunit protein uS13 (chains M)
MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVKISELSEGQIDTLRDEV
AKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRTKTNARTRKGPRKPIKK

Sequence of entity 9 (N), FASTA

>8CAZ_9 Small ribosomal subunit protein uS14 (chains N)
MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNAVLKLQTLPRDSSPSRQ
RNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW

Sequence of entity 10 (S), FASTA

>8CAZ_10 Small ribosomal subunit protein uS19 (chains S)
MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNMIGLTIAVHNGRQHVPV
FVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	29

Water and common crystallization additives (K) are not listed.

Primary citation

Structural conservation of antibiotic interaction with ribosomes. Paternoga, H., Crowe-McAuliffe, C., Bock, L.V. et al. Nat Struct Mol Biol (2023) 30:1380-1392. DOI 10.1038/s41594-023-01047-y · PubMed

Other PDB entries of the same protein (UniProt P0A7M9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8FTO 1.85 Å, E. coli 70S ribosome with an improved MS2 tag inserted in H98
9GHE 1.87 Å, Staphylococcus aureus FusB bound to the small subunit of the Escherichia coli 70S…
9T0Y 1.87 Å, E. coli 70S ribosome from delta-9 strain
9T6M 1.87 Å, E. coli 70S ribosome from delta-10 strain
8CGI 1.89 Å, Pentacycline TP038 bound to the 30S head
9SYH 1.89 Å, E. coli 70S ribosome from deltaRlmE strain
8AYE 1.96 Å, E. coli 70S ribosome bound to thermorubin and fMet-tRNA
7K00 1.98 Å, Structure of the Bacterial Ribosome at 2 Angstrom Resolution
9Q3R 1.99 Å, Cryo-EM structure of the Escherichia coli 70S ribosome bound to mRNA, P-site…
8QOA 2.0 Å, Structure of SecM-stalled Escherichia coli 70S ribosome
9Q3Q 2.01 Å, Cryo-EM structure of translating Escherichia coli 70S ribosome bound to mRNA, P-site…
8G6W 2.02 Å, Structure of WT E.coli 70S ribosome complexed with mRNA, P-site fMet-NH-tRNAfMet and…

Browse structure collections

About this viewer

MolViewer shows 8CAZ directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.