8D4G: AP-1 complex subunit beta-1

gamma-Arf1 mediated dimeric assembly of AP-1, Arf1, Nef complex within lattice on MHC-I lipopeptide incorporated wide(r) membrane tubes. Determined by electron microscopy at 11.6 Å resolution. Released 14 Jun 2023.

Method: Electron microscopy
Resolution: 11.6 Å
Organisms: Homo sapiens, Mus musculus, Human immunodeficiency virus 1
Chains: 20
Atoms: 19,534
Mol. weight: 712.72 kDa
Ligands: MG, GTP
Released: 14 Jun 2023

Explore 8D4G in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8D4G contains 256 α-helices and 148 β-strands across 18 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A and B: 41 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	15-22	8
α-helix	27-43	17
α-helix	48-50	3
α-helix	51-56	6
α-helix	63-75	13
α-helix	83-87	5
α-helix	88-94	7
α-helix	100-112	13
α-helix	116-121	6
α-helix	123-130	8
α-helix	135-151	17
α-helix	153-159	7
α-helix	161-169	9
α-helix	174-190	17
α-helix	201-211	11
α-helix	216-228	13
α-helix	234-244	11
α-helix	245-249	5
β-strand	250	1	20
α-helix	253-267	15
α-helix	275-292	18
α-helix	296-310	15
α-helix	326-327	2
α-helix	332-344	13
α-helix	351-361	11
α-helix	367-383	17
α-helix	385-387	3
α-helix	388-400	13
α-helix	404-420	17
α-helix	429-433	5
α-helix	442-454	13
α-helix	462-469	8
α-helix	473-475	3
α-helix	478-494	17
α-helix	500-511	12
α-helix	517-532	16
α-helix	534-540	7
α-helix	550-552	3
α-helix	555-556	2
α-helix	557-565	9
β-strand	569	1	21
α-helix	570-574	5
α-helix	578-580	3

Chains C, D, F, H, Q and Z: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	5-12	8
β-strand	19-24	6	3
α-helix	30-38	9
β-strand	51-58	8	3
β-strand	61-68	8	3
α-helix	78-81	4
β-strand	89-91	3	3
α-helix	97-111	15
α-helix	120-121	2
β-strand	122-125	4	3
α-helix	138-143	6
α-helix	145-147	3
β-strand	154-157	4	3
α-helix	166-177	12

Chains E and G: 35 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	7-16	10
α-helix	20-39	20
α-helix	46-59	14
α-helix	68-74	7
α-helix	79-92	14
α-helix	103-111	9
α-helix	116-128	13
α-helix	132-146	15
α-helix	151-167	17
α-helix	169-175	7
α-helix	188-204	17
α-helix	207-212	6
α-helix	216-228	13
β-strand	236-237	2	23
β-strand	240-241	2	23
α-helix	243-255	13
α-helix	261-277	17
α-helix	283-298	16
α-helix	303-317	15
α-helix	322-334	13
α-helix	340-343	4
α-helix	344-346	3
α-helix	347-354	8
α-helix	359-371	13
α-helix	378-390	13
α-helix	394-411	18
α-helix	415-429	15
α-helix	430-432	3
α-helix	437-447	11
α-helix	453-465	13
α-helix	470-487	18
α-helix	504-514	11
α-helix	522-536	15
α-helix	544-551	8
α-helix	557-572	16
α-helix	578-580	3
α-helix	582-585	4

Chains J and M: 13 helices, 33 β-strands

Element	Residues	Length	Sheet
β-strand	3-8	6	25
β-strand	17-20	4	25
α-helix	29-43	15
β-strand	49	1	25
β-strand	52	1	26
β-strand	55	1	26
β-strand	56-61	6	25
β-strand	66-71	6	25
β-strand	76	1	21
α-helix	77-94	18
α-helix	100-103	4
α-helix	107-117	11
β-strand	118-119	2	27
β-strand	122-123	2	27
α-helix	128-134	7
α-helix	148-150	3
α-helix	151-154	4
β-strand	170-183	14	20
β-strand	189-203	15	20
β-strand	209-214	6	28
β-strand	216	1	29
α-helix	217-223	7
β-strand	231	1	29
β-strand	235-238	4	20
β-strand	242	1	28
α-helix	244-250	7
β-strand	253-255	3	28
α-helix	256-258	3
β-strand	260-270	11	20
α-helix	274-275	2
β-strand	277-280	4	30
β-strand	283	1	30
β-strand	290-299	10	30
β-strand	306-314	9	20
β-strand	321-327	7	30
β-strand	331-335	5	20
β-strand	340-349	10	20
β-strand	353-361	9	30
α-helix	366-367	2
α-helix	374-375	2
β-strand	376-378	3	20
β-strand	381-383	3	20
β-strand	385	1	31
β-strand	392-398	7	28
β-strand	408-415	8	20
β-strand	419-420	2	20

Chains K and N: 9 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	7-22	16
β-strand	69	1	31
β-strand	76	1	32
β-strand	77-78	2	33
α-helix	79-80	2
α-helix	81-94	14
α-helix	100	1
β-strand	101	1	34
α-helix	102	1
α-helix	104-118	15
β-strand	120-121	2	33
β-strand	127	1	35
β-strand	134	1	34
β-strand	136	1	35
β-strand	143-147	5	34
β-strand	181-185	5	34
α-helix	187-191	5
α-helix	194-198	5
α-helix	200-203	4

Chains O and S: 5 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	2-8	7	36
β-strand	14-19	6	36
α-helix	25-40	16
β-strand	49-52	4	36
β-strand	55-59	5	36
β-strand	66-71	6	36
α-helix	77-95	19
α-helix	100-105	6
α-helix	107-117	11
β-strand	118-119	2	37
β-strand	122-123	2	37
α-helix	129-141	13

Chains P and Y: 1 helix, 2 β-strands

Element	Residues	Length	Sheet
β-strand	345-346	2	20
β-strand	347	1	32
α-helix	352-354	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
AP-1 complex subunit beta-1	A, B	protein	949	Homo sapiens	Q10567 (AlphaFold model)
ADP-ribosylation factor 1	C, D, F, H, Q, Z	protein	181	Homo sapiens	P84077 (AlphaFold model)
AP-1 complex subunit gamma-1	E, G	protein	601	Mus musculus	P22892 (AlphaFold model)
Protein Nef	I, K, L, N	protein	213	Human immunodeficiency virus 1	Q90VU7
AP-1 complex subunit mu-1	J, M	protein	423	Mus musculus	P35585
AP-1 complex subunit sigma-3	O, S	protein	154	Homo sapiens	Q96PC3
HLA class I histocompatibility antigen, A alpha chain	P, Y	protein	39	Homo sapiens	P04439

Sequence of entity 1 (A, B), FASTA

>8D4G_1 AP-1 complex subunit beta-1 (chains A, B)
MTDSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQT
DNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITE
YLCEPLRKCLKDEDPYVRKTAAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANA
VAALSEIAESHPSSNLLDLNPQSINKLLTALNECTEWGQIFILDCLANYMPKDDREAQSI
CERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYGTLLKKLAPPLVTLLSAEPELQY
VALRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQANIAQVLAELRE
YATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRK
YPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLEGFHDKSTQV
QLQLLTAIVKLFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEV
VLAEKPLISEETDLIEPTLLDELICYIGTLASVYHKPPSAFVEGGRGVVHKSLPPRTASS
ESAESPETAPTGAPPGEQPDVIPAQGDLLGDLLNLDLGPPVSGPPLATSSVQMGAVDLLG
GGLDSLMGDEPEGIGGTNFVAPPTAAVPANLGAPIGSGLSDLFDLTSGVGTLSGSYVAPK
AVWLPAMKAKGLEISGTFTRQVGSISMDLQLTNKALQVMTDFAIQFNRNSFGLAPAAPLQ
VHAPLSPNQTVEISLPLSTVGSVMKMEPLNNLQVAVKNNIDVFYFSTLYPLHILFVEDGK
MDRQMFLATWKDIPNENEAQFQIRDCPLNAEAASSKLQSSNIFTVAKRNVEGQDMLYQSL
KLTNGIWVLAELRIQPGNPSCTDLELSLKCRAPEVSQHVYQAYETILKN

Sequence of entity 2 (C, D, F, H, Q, Z), FASTA

>8D4G_2 ADP-ribosylation factor 1 (chains C, D, F, H, Q, Z)
MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKN
ISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAV
LLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQ
K

Sequence of entity 3 (E, G), FASTA

>8D4G_3 AP-1 complex subunit gamma-1 (chains E, G)
MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLG
YPAHFGQLECLKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQG
LALCTLGCMGSSEMCRDLAGEVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATK
NLLNEKNHGVLHTSVVLLTEMCERSPDMLAHFRKLVPQLVRILKNLIMSGYSPEHDVSGI
SDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNTETSKNVGNAILYETVLTIMDIK
SESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHRSTIVDCLKDLDVS
IKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRWHID
TIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCI
GEYGDLLVSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCT
VNRIKKVVSIYGSSIDVELQQRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPENLYF
Q

Sequence of entity 4 (I, K, L, N), FASTA

>8D4G_4 Protein Nef (chains I, K, L, N)
MGGKWSKSSVIGWPAVRERMRRAEPAADGVGAVSRDLEKHGAITSSNTAANNAACAWLEA
QEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGY
FPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLHPVSLHGMDDPEREV
LEWRFDSRLAFHHVARELHPEYFKNCGHHHHHH

Sequence of entity 5 (J, M), FASTA

>8D4G_5 AP-1 complex subunit mu-1 (chains J, M)
MSASAVYVLDLKGKVLICRNYRGDVDMSEVEHFMPILMEKEEEGMLSPILAHGGVRFMWI
KHNNLYLVATSKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDF
GYPQTTDSKILQEYITQEGHKLETGAPRPPATVTNAVSWRSEGIKYRKNEVFLDVIEAVN
LLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFDNTGRGKSKSVELEDVKFHQ
CVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMVKAKSQ
FKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSVKSFPGGKEYLMRAHF
GLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQL
RTQ

Sequence of entity 6 (O, S), FASTA

>8D4G_6 AP-1 complex subunit sigma-3 (chains O, S)
MIHFILLFSRQGKLRLQKWYITLPDKERKKITREIVQIILSRGHRTSSFVDWKELKLVYK
RYASLYFCCAIENQDNELLTLEIVHRYVELLDKYFGNVCELDIIFNFEKAYFILDEFIIG
GEIQETSKKIAVKAIEDSDMLQEVSTVSQTMGER

Sequence of entity 7 (P, Y), FASTA

>8D4G_7 HLA class I histocompatibility antigen, A alpha chain (chains P, Y)
CRKSSDRKGGSYSQAAGSDSAQSSDVSLTAAKVHHHHHH

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	6
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	6

Primary citation

Self-assembly and structure of a clathrin-independent AP-1:Arf1 tubular membrane coat. Hooy, R.M., Iwamoto, Y., Tudorica, D.A. et al. Sci Adv (2022) 8:eadd3914-eadd3914. DOI 10.1126/sciadv.add3914 · PubMed

Other PDB entries of the same protein (UniProt Q10567 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7R4H 2.34 Å, phospho-STING binding to adaptor protein complex-1
4P6Z 3.0 Å, Crystal structure of the human BST2 cytoplasmic domain and the HIV-1 Vpu cytoplasmic…
6CM9 3.73 Å, Structure of the cargo bound AP-1:Arf1:tetherin-Nef closed trimer monomeric subunit
6DFF 3.9 Å, Structure of the cargo bound AP-1:Arf1:tetherin-Nef monomer
6D83 4.27 Å, Structure of the cargo bound AP-1:Arf1:tetherin-Nef (L164A, L165A) dileucine mutant…
6D84 6.72 Å, Structure of the cargo bound AP-1:Arf1:tetherin-Nef (L164A, L165A) dileucine mutant dimer
6CRI 6.8 Å, Structure of the cargo bound AP-1:Arf1:tetherin-Nef stable closed trimer
4HMY 7.0 Å, Structural basis for recruitment and activation of the AP-1 clathrin adaptor complex by…
8D4E 9.2 Å, Asymmetric unit of AP-1, Arf1, Nef lattice on MHC-I lipopeptide incorporated wide(r)…
8D4C 9.3 Å, beta-Arf1 mediated dimeric assembly of AP-1, Arf1, Nef complex within lattice on MHC-I…
8D9W 9.3 Å, beta-Arf1 homodimeric interface within AP-1, Arf1, Nef, MHC-I lattice on narrow tubes
8D9V 9.4 Å, gamma-Arf1 homodimeric interface within AP-1, Arf1, Nef lattice on narrow membrane tubes

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