8EIU: 50S ribosomal protein L33

Name: PDB 8EIU: E. coli 70S ribosome with A-loop mutations U2554C and U2555C
Published: 2023-02-01
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: ribosome

E. coli 70S ribosome with A-loop mutations U2554C and U2555C. Determined by electron microscopy at 2.24 Å resolution. Released 1 Feb 2023.

Method: Electron microscopy
Resolution: 2.24 Å
Organism: Escherichia coli
Chains: 55
Atoms: 148,825
Mol. weight: 2208.41 kDa
Ligands: ZN, PAR, MG, SPD
Released: 1 Feb 2023

Explore 8EIU in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8EIU contains 248 α-helices and 300 β-strands across 49 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 0: 2 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	6	1
β-strand	7-13	7	1
β-strand	20-25	6	1
α-helix	33-34	2
β-strand	35-40	6	1
β-strand	45-52	8	1

Chain 1: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	9-16	8
α-helix	18-22	5
α-helix	25-37	13

Chain 2: 5 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	3-6	4
α-helix	8-11	4
β-strand	15-16	2	3
β-strand	22-24	3	3
α-helix	25-26	2
α-helix	38-44	7
β-strand	48-49	2	3
α-helix	52-61	10

Chain 3: 1 helix, 4 β-strands

Element	Residues	Length	Sheet
β-strand	2-4	3	6
β-strand	14-19	6	6
β-strand	22-27	6	6
α-helix	31-33	3
β-strand	35-37	3	6

Chain 4: 3 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	7-8	2
β-strand	10-15	6	7
β-strand	21-26	6	7
β-strand	32-33	2	7
α-helix	42-44	3
α-helix	57-63	7

Chain B: 9 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	6-12	7
β-strand	16-19	4	17
α-helix	20-22	3
α-helix	25-30	6
β-strand	31-34	4	17
β-strand	38-41	4	17
α-helix	43-62	20
β-strand	67-70	4	18
α-helix	77-85	9
β-strand	90-92	3	18
α-helix	104-121	18
α-helix	133-148	16
β-strand	160-164	5	18
α-helix	170-178	9
β-strand	183-187	5	18
β-strand	198-201	4	18
α-helix	207-226	20

Chain c: 7 helices, 28 β-strands

Element	Residues	Length	Sheet
β-strand	3-5	3	8
α-helix	6-7	2
β-strand	17-19	3	8
β-strand	24	1	9
α-helix	31-33	3
β-strand	34-36	3	10
β-strand	43	1	11
β-strand	49	1	11
β-strand	54	1	12
β-strand	61-63	3	10
β-strand	76-82	7	9
β-strand	91-96	6	9
β-strand	101-105	5	9
β-strand	107	1	13
β-strand	115-116	2	9
β-strand	118	1	14
β-strand	129-131	3	14
β-strand	140-142	3	14
β-strand	144-145	2	15
β-strand	152-154	3	15
β-strand	162-168	7	14
β-strand	171-175	5	14
β-strand	181-185	5	14
β-strand	189-192	4	14
β-strand	195	1	13
α-helix	198-202	5
β-strand	204	1	8
α-helix	208-213	6
β-strand	216	1	12
α-helix	217-221	5
α-helix	222-224	3
β-strand	245	1	16
β-strand	251	1	16
α-helix	264-266	3
β-strand	267-269	3	14

Chain C: 8 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	9-11	3
β-strand	20-21	2	23
α-helix	26-46	21
β-strand	52-58	7	23
β-strand	64-70	7	23
α-helix	73-77	5
α-helix	82-95	14
β-strand	99-105	7	23
α-helix	109-111	3
α-helix	113-125	13
α-helix	130-143	14
β-strand	148-155	8	24
α-helix	157-159	3
β-strand	164-171	8	24
β-strand	182-191	10	24
β-strand	194-203	10	24

41 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
50S ribosomal protein L33	0	protein	55	Escherichia coli	P0A7N9 (AlphaFold model)
50S ribosomal protein L34	1	protein	46	Escherichia coli	P0A7P5 (AlphaFold model)
50S ribosomal protein L35	2	protein	65	Escherichia coli	P0A7Q1 (AlphaFold model)
50S ribosomal protein L36	3	protein	38	Escherichia coli	P0A7Q6 (AlphaFold model)
50S ribosomal protein L31	4	protein	70	Escherichia coli	P0A7M9
16S rRNA	A	RNA	1542	Escherichia coli
30S ribosomal protein S2	B	protein	241	Escherichia coli	P0A7V0
30S ribosomal protein S3	C	protein	233	Escherichia coli	P0A7V3
30S ribosomal protein S4	D	protein	206	Escherichia coli	P0A7V8
30S ribosomal protein S5	E	protein	167	Escherichia coli	P0A7W1
30S ribosomal protein S6	F	protein	135	Escherichia coli	P02358
30S ribosomal protein S7	G	protein	179	Escherichia coli	P02359

42 more molecules are not listed.

Sequence of entity 1 (0), FASTA

>8EIU_1 50S ribosomal protein L33 (chains 0)
MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVRQHVIYKEAKIK

Sequence of entity 2 (1), FASTA

>8EIU_2 50S ribosomal protein L34 (chains 1)
MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK

Sequence of entity 3 (2), FASTA

>8EIU_3 50S ribosomal protein L35 (chains 2)
MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHLRPKAMVSKGDLGLVIA
CLPYA

Sequence of entity 4 (3), FASTA

>8EIU_4 50S ribosomal protein L36 (chains 3)
MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG

Sequence of entity 5 (4), FASTA

>8EIU_5 50S ribosomal protein L31 (chains 4)
MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFFTGKQRDVATGGRVDRF
NKRFNIPGSK

Sequence of entity 6 (A), FASTA

>8EIU_6 16S rRNA (chains A)
AAAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAA
GUCGAACGGUAACAGGAAGAAGCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAA
UGUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAAACGGUAGCUAAUACCGCAU
AACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUG
GGAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGA
GGAUGACCAGCCACACUGGAACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGG
GGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGCCGCGUGUAUGAAGAAGGCCU
UCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUU
GACGUUACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAGCAGCCGCGGUAAUACGGAG
GGUGCAAGCGUUAAUCGGAAUUACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCA
GAUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGAUACUGGCAAGCUUGAGUCUC
GUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACC
GGUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCA
AACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCC
CUUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACCGCCUGGGGAGUACGGCCGCA
AGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAU
UCGAUGCAACGCGAAGAACCUUACCUGGUCUUGACAUCCACGGAAGUUUUCAGAGAUGAG
AAUGUGCCUUCGGGAACCGUGAGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGA
AAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCCUUUGUUGCCAGCGGUCCGGC
CGGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUC
AUCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCG
ACCUCGCGAGAGCAAGCGGACCUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAAC
UCGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCAGAAUGCCACGGUGAAUACGU
UCCCGGGCCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGU
AGCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGAAGUCGUAA
CAAGGUAACCGUAGGGGAACCUGCGGUUGGAUCACCUCCUUA

Sequence of entity 7 (B), FASTA

>8EIU_7 30S ribosomal protein S2 (chains B)
MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEALAELNKI
ASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLTNWKTVRQSIKRLKDLETQ
SQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDALFVIDADHEHIAIKEANNLG
IPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVAATVREGRSQDLASQAEESFVEA
E

Sequence of entity 8 (C), FASTA

>8EIU_8 30S ribosomal protein S3 (chains C)
MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLTKELAKASVSRIVIERP
AKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQINIAEVRKPELDAKLVADSI
TSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGAEIARTEWYREGRVPLHTLRA
DIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPEKPAAQPKKQQRKGRK

Sequence of entity 9 (D), FASTA

>8EIU_9 30S ribosomal protein S4 (chains D)
MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQK
VRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSH
KAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALELAEQREKPTWLEVDAGKMEG
TFKRKPERSDLSADINEHLIVELYSK

Sequence of entity 10 (E), FASTA

>8EIU_10 30S ribosomal protein S5 (chains E)
MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNGRVGFGYGKAREVPAAI
QKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEGTGIIAGGAMRAVLEVAGV
HNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKSVEEILGK

Sequence of entity 11 (F), FASTA

>8EIU_11 30S ribosomal protein S6 (chains F)
MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYV
LMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPMVKAKDERRERRDDFANET
ADDAEAGDSEEEEEE

Sequence of entity 12 (G), FASTA

>8EIU_12 30S ribosomal protein S7 (chains G)
MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELE
AFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRL
ANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRWLSLRSFSHQAGASSKQPALGYLN

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	2
PAR	Paromomycin	C23 H45 N5 O14	1
MG	Magnesium ion	Mg	268
SPD	Spermidine	C7 H19 N3	16
8AN	3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate)	C10 H15 N6 O6 P	2
SPM	Spermine	C10 H26 N4	1
MET	Methionine	C5 H11 N O2 S	2

Primary citation

Rare ribosomal RNA sequences from archaea stabilize the bacterial ribosome. Nissley, A.J., Penev, P.I., Watson, Z.L. et al. Nucleic Acids Res (2023) 51:1880-1894. DOI 10.1093/nar/gkac1273 · PubMed

Other PDB entries of the same protein (UniProt P0A7N9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8B0X 1.55 Å, Translating 70S ribosome in the unrotated state (P and E, tRNAs)
9Q87 1.55 Å, Principles of ion binding to RNA inferred from the analysis of a 1.55 Angstrom…
8CGK 1.64 Å, Lincomycin and Avilamycin bound to the 50S subunit
8CGV 1.66 Å, Tiamulin bound to the 50S subunit
8CEU 1.83 Å, Retapamulin and Capreomycin bound to the 50S subunit
8FTO 1.85 Å, E. coli 70S ribosome with an improved MS2 tag inserted in H98
8CAM 1.86 Å, Evernimicin bound to the 50S subunit
9GHE 1.87 Å, Staphylococcus aureus FusB bound to the small subunit of the Escherichia coli 70S…
9T0Y 1.87 Å, E. coli 70S ribosome from delta-9 strain
9T6M 1.87 Å, E. coli 70S ribosome from delta-10 strain
9SYH 1.89 Å, E. coli 70S ribosome from deltaRlmE strain
9D89 1.95 Å, E. coli 50S ribosomal subunit in complex with PrAMP rumicidin-2 (focused refinement)

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