8FB7: Ky15.10 Fab

Crystal structure of Ky15.10 Fab in complex with circumsporozoite protein NPDP peptide. Determined by X-ray diffraction at 1.75 Å resolution. Released 1 Nov 2023.

Method: X-ray diffraction
Resolution: 1.75 Å
Organisms: Mus musculus, Plasmodium falciparum (isolate NF54)
Chains: 6
Atoms: 7,927
Mol. weight: 99.98 kDa
Released: 1 Nov 2023

Explore 8FB7 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8FB7 contains 35 α-helices and 88 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	1
β-strand	11-12	2	2
β-strand	18-25	8	1
α-helix	29-31	3
β-strand	34-39	6	3
β-strand	45-51	7	3
β-strand	57-59	3	3
β-strand	68-72	5	1
α-helix	73-75	3
β-strand	77-82	6	1
α-helix	84-86	3
β-strand	88-95	8	3
α-helix	96-97	2
β-strand	100H-103	4	3
β-strand	107-109	3	3
β-strand	110-111	2	2
α-helix	114-116	3
β-strand	117	1	4
α-helix	118-119	2
β-strand	120-124	5	5
β-strand	131-132	2	5
β-strand	135-145	11	5
β-strand	146	1	4
β-strand	151-154	4	6
α-helix	155-157	3
β-strand	159	1	6
β-strand	163-165	3	5
α-helix	166-168	3
β-strand	169-170	2	5
β-strand	176-185	10	5
α-helix	186-188	3
β-strand	195-200	6	6
α-helix	201-203	3
β-strand	205-210	6	6

Chain B: 7 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	7
β-strand	10-13	4	8
β-strand	19-25	7	7
β-strand	33-38	6	8
β-strand	45-49	5	8
β-strand	53-54	2	8
α-helix	55	1
β-strand	62-66	5	7
β-strand	70-75	6	7
α-helix	80-82	3
β-strand	84-90	7	8
β-strand	97-98	2	8
β-strand	102-106	5	8
β-strand	111	1	9
α-helix	112-113	2
β-strand	114-118	5	10
α-helix	119-121	3
α-helix	122-126	5
β-strand	129-139	11	10
β-strand	140	1	9
β-strand	145-150	6	11
β-strand	153-154	2	11
β-strand	159-163	5	10
α-helix	164-167	4
β-strand	173-182	10	10
α-helix	183-187	5
β-strand	191-197	7	11
β-strand	205-210	6	11

Chain H: 10 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	12
β-strand	11-12	2	13
β-strand	18-25	8	12
α-helix	29-31	3
β-strand	34-39	6	14
β-strand	45-51	7	14
β-strand	57-59	3	14
β-strand	67-72	6	12
α-helix	73-75	3
β-strand	77-82	6	12
α-helix	84-86	3
β-strand	88-95	8	14
α-helix	96-97	2
β-strand	100H-103	4	14
β-strand	107-109	3	14
β-strand	110-111	2	13
α-helix	114-116	3
β-strand	117	1	15
α-helix	118-119	2
β-strand	120-124	5	16
β-strand	135-145	11	16
β-strand	146	1	15
β-strand	151-154	4	17
α-helix	155-157	3
β-strand	163-165	3	16
α-helix	166-168	3
β-strand	169-170	2	16
β-strand	176-185	10	16
α-helix	186-188	3
β-strand	194-200	7	17
α-helix	201-203	3
β-strand	205-211	7	17

Chain L: 8 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	18
β-strand	10-13	4	14
β-strand	19-25	7	18
β-strand	33-38	6	14
β-strand	45-49	5	14
β-strand	53-54	2	14
α-helix	55	1
β-strand	62-67	6	18
β-strand	70-75	6	18
α-helix	80-82	3
β-strand	85-90	6	14
β-strand	97-98	2	14
β-strand	102-106	5	14
β-strand	111	1	19
α-helix	112-113	2
β-strand	114-118	5	20
α-helix	119-121	3
α-helix	122-127	6
β-strand	129-139	11	20
β-strand	140	1	19
β-strand	145-150	6	21
β-strand	153-154	2	21
α-helix	155	1
β-strand	159-163	5	20
α-helix	164-167	4
β-strand	173-182	10	20
α-helix	183-187	5
β-strand	191-197	7	21
β-strand	205-210	6	21

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Ky15.10 Antibody, Heavy Chain	A, H	protein	228	Mus musculus
Ky15.10 Antibody, Light chain	B, L	protein	213	Mus musculus
Circumsporozoite protein NPDP peptide	P, Q	protein	16	Plasmodium falciparum (isolate NF54)	P19597 (AlphaFold model)

Sequence of entity 1 (A, H), FASTA

>8FB7_1 Ky15.10 Antibody, Heavy Chain (chains A, H)
QVQLVESGGGVVQPGRSLRLSCAASGFTFSNSGMHWVRQVPGKGLEWVAIIWYDGSNKYY
ADSVKGRFSVSRDNSENTLYLQMSNLRAEDTAVYYCVRAYFDSENLYDYYGMDVWGQGTT
VTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPA
VLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC

Sequence of entity 2 (B, L), FASTA

>8FB7_2 Ky15.10 Antibody, Light chain (chains B, L)
DIQMTQSPSTLSASVGDRVTITCRASQSISRWLAWFQKKPGKAPKLLIYTASNLESGVPS
RFSGSGSGTEFTLTISSLQPDDFATYYCQQYYNYWTFGQGTKVEVKRTVAAPSVFIFPPS
DEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTL
SKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

Sequence of entity 3 (P, Q), FASTA

>8FB7_3 Circumsporozoite protein NPDP peptide (chains P, Q)
NPDPNANPNVDPNANP

Primary citation

Molecular determinants of cross-reactivity and potency by VH3-33 antibodies against the Plasmodium falciparum circumsporozoite protein. Thai, E., Murugan, R., Binter, S. et al. Cell Rep (2023) 42:113330-113330. DOI 10.1016/j.celrep.2023.113330 · PubMed

Other PDB entries of the same protein (UniProt P19597 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6O24 1.4 Å, Crystal structure of 4498 Fab in complex with circumsporozoite protein NANP3 and…
8FDD 1.54 Å, Crystal structure of Ky15.3 Fab in complex with circumsporozoite protein NPDP peptide
8FAS 1.55 Å, Crystal structure of Ky230 Fab in complex with circumsporozoite protein NANP5 peptide
6VLN 1.63 Å, Crystal structure of 4498 Fab in complex with circumsporozoite protein DND3 and…
8FB8 1.69 Å, Crystal structure of Ky15.10-Y100EK Fab in complex with circumsporozoite protein KQPA…
6ULF 1.7 Å, Crystal structure of 4498 Fab in complex with circumsporozoite protein NDN3 and…
8F9U 1.7 Å, Crystal structure of Ky15.7 Fab in complex with circumsporozoite protein NPDP peptide
6O26 1.8 Å, Crystal structure of 3246 Fab in complex with circumsporozoite protein NANA
8FA7 1.8 Å, Crystal structure of Ky15.11 Fab in complex with circumsporozoite protein KQPA peptide
8FA8 1.8 Å, Crystal structure of Ky15.11 Fab in complex with circumsporozoite protein NDN peptide
8FDC 1.8 Å, Crystal structure of Ky311 Fab in complex with circumsporozoite protein KQPA peptide
8F9T 1.85 Å, Crystal structure of Ky15.2 Fab in complex with circumsporozoite protein NPDP peptide

Browse structure collections

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