Structure of human NDS.1 Fab and 1G01 Fab in complex with influenza virus neuraminidase from A/Indiana/10/2011 (H3N2v), based on consensus cryo-EM map with only Fab 1G01 resolved. Determined by electron microscopy at 3.0 Å resolution. Released 28 Feb 2024.
Explore 8GAT in 3D Show helices and sheets RCSB PDB PDBe
8GAT contains 8 α-helices and 56 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 90 | 1 | |
| β-strand | 91 | 1 | 1 |
| α-helix | 92-93 | 2 | |
| β-strand | 96-102 | 7 | 1 |
| α-helix | 105-108 | 4 | |
| β-strand | 115-124 | 10 | 2 |
| β-strand | 129-139 | 11 | 2 |
| α-helix | 143-145 | 3 | |
| β-strand | 157-162 | 6 | 2 |
| α-helix | 171 | 1 | |
| β-strand | 172-176 | 5 | 2 |
| β-strand | 179-184 | 6 | 3 |
| β-strand | 189-195 | 7 | 3 |
| β-strand | 202-207 | 6 | 3 |
| β-strand | 210-216 | 7 | 3 |
| β-strand | 223-224 | 2 | 4 |
| β-strand | 229 | 1 | 3 |
| β-strand | 231-233 | 3 | 4 |
| β-strand | 236-244 | 9 | 4 |
| β-strand | 250-258 | 9 | 4 |
| β-strand | 261-267 | 7 | 4 |
| β-strand | 276-283 | 8 | 5 |
| β-strand | 286-292 | 7 | 5 |
| β-strand | 301-306 | 6 | 5 |
| β-strand | 312-316 | 5 | 5 |
| β-strand | 319 | 1 | 6 |
| β-strand | 353-356 | 4 | 7 |
| β-strand | 359-364 | 6 | 7 |
| β-strand | 372-379 | 8 | 7 |
| β-strand | 388 | 1 | 6 |
| β-strand | 391-403 | 13 | 7 |
| β-strand | 407-413 | 7 | 1 |
| β-strand | 418-425 | 8 | 1 |
| β-strand | 428-429 | 2 | 8 |
| β-strand | 439-440 | 2 | 8 |
| β-strand | 443-449 | 7 | 1 |
| α-helix | 464-466 | 3 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 3-7 | 5 | 9 |
| β-strand | 11 | 1 | 10 |
| β-strand | 18-25 | 8 | 9 |
| β-strand | 33-39 | 7 | 11 |
| β-strand | 45-51 | 7 | 11 |
| β-strand | 57-59 | 3 | 11 |
| α-helix | 61-63 | 3 | |
| β-strand | 67-72 | 6 | 9 |
| β-strand | 77-82 | 6 | 9 |
| β-strand | 89-96 | 8 | 11 |
| β-strand | 100L-101 | 3 | 11 |
| β-strand | 102 | 1 | 12 |
| β-strand | 103 | 1 | 11 |
| β-strand | 107-108 | 2 | 11 |
| β-strand | 110 | 1 | 10 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 4-7 | 4 | 13 |
| β-strand | 10-13 | 4 | 12 |
| β-strand | 19-25 | 7 | 13 |
| β-strand | 34-38 | 5 | 12 |
| β-strand | 45-49 | 5 | 12 |
| β-strand | 53-54 | 2 | 12 |
| α-helix | 55 | 1 | |
| β-strand | 62-65 | 4 | 13 |
| β-strand | 70-75 | 6 | 13 |
| β-strand | 85-89 | 5 | 12 |
| β-strand | 98 | 1 | 12 |
| β-strand | 103-106 | 4 | 12 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Vasodilator-stimulated phosphoprotein, Neuraminidase chimera | A | protein | 466 | Homo sapiens, Influenza A virus (A/Indiana/10/2011(H3N2)) | G9LQ08, P50552 (AlphaFold model) |
| Fab 1G01, heavy chain | M | protein | 240 | Homo sapiens | |
| Fab 1G01, light chain | N | protein | 216 | Homo sapiens |
>8GAT_1 Vasodilator-stimulated phosphoprotein, Neuraminidase chimera (chains A) MEFGLSWIFLAAILKGVQCADPHHHHHHSSSDYSDLQRVKQELLEEVKKELQKVKEEIIE AFVQELRKRGSLVPRGSGGEYRNWSKPQCNITGFAPFSKDNSIRLSAGGDIWVTREPYVS CDPDKCYQFALGQGTTLNNGHSNNTVHDRTPYRTLLMNELGVPFHLGTRQVCMAWSSSSC HDGKAWLHVCITGNDNNATASFIYNGRLVDSIGSWSKNILRTQESECVCINGTCTVVMTD GSASGKADTKILFVEEGKIVHISTLSGSAQHVEECSCYPRFPGVRCVCRDNWKGSNRPIV DINVKNYSIVSSYVCSGLVGDTPRKSDSVSSSYCLDPNNEKGGHGVKGWAFDDGNDVWMG RTINETLRLGYETFKVIEGWSKANSKLQTNRQVIVEKGDRSGYSGIFSVEGKSCINRCFY VELIRGRKEETKVWWTSNSIVVFCGTSGTYGTGSWPDGADINLMPI
>8GAT_2 Fab 1G01, heavy chain (chains M) DEVQLVESGGRALRPGGSLRLSCAASGFKFDDYAMSWVRQVPGKGLEFVSGLNWNGDITA YTDSVKGRFTVSRDNAKNSLYLHINSPKPEDTALYYCARTSSWGDYTRGPEPKITWYFDL WGRGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSG VHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCHHHHHH
>8GAT_3 Fab 1G01, light chain (chains N) DDIQLTQSPSFLSASVGDRITITCRASQGIDGYLAWYQQRPGKAPNLLIYAASLLQSGVP SRFSGSGYGTEFTLTISSLQPEDFATYYCQHLDSYPLFTFGPGTKVDIKRTVAAPSVFIF PPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSST LTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
| ID | Name | Formula | Copies |
|---|---|---|---|
| NAG | 2-acetamido-2-deoxy-beta-D-glucopyranose | C8 H15 N O6 | 4 |
Protective human monoclonal antibodies target conserved sites of vulnerability on the underside of influenza virus neuraminidase. Lederhofer, J., Tsybovsky, Y., Nguyen, L. et al. Immunity (2024) 57:574-586.e7. DOI 10.1016/j.immuni.2024.02.003 · PubMed
Other PDB entries of the same protein (UniProt G9LQ08), best resolution first:
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