Cryo-EM structure of 5-subunit Smc5/6 arm region. Determined by electron microscopy at 5.97 Å resolution. Released 26 Jun 2024.
Explore 8I4V in 3D Show helices and sheets RCSB PDB PDBe
8I4V contains 29 α-helices and 5 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 233-267 | 35 | |
| α-helix | 272-363 | 92 | |
| α-helix | 747-768 | 22 | |
| α-helix | 771-780 | 10 | |
| α-helix | 783-824 | 42 | |
| α-helix | 828-832 | 5 | |
| α-helix | 836-842 | 7 | |
| α-helix | 847-862 | 16 | |
| α-helix | 867-881 | 15 | |
| α-helix | 891-903 | 13 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 288-400 | 113 | |
| α-helix | 792-881 | 90 | |
| α-helix | 897-918 | 22 | |
| α-helix | 922-929 | 8 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 16-24 | 9 | |
| α-helix | 27-29 | 3 | |
| α-helix | 31-51 | 21 | |
| α-helix | 60-100 | 41 | |
| α-helix | 102-104 | 3 | |
| α-helix | 108-114 | 7 | |
| α-helix | 122-128 | 7 | |
| α-helix | 130-132 | 3 | |
| α-helix | 142-155 | 14 | |
| β-strand | 195-197 | 3 | 1 |
| β-strand | 203-204 | 2 | 1 |
| α-helix | 209-213 | 5 | |
| β-strand | 219-220 | 2 | 2 |
| β-strand | 229-230 | 2 | 2 |
| β-strand | 235-237 | 3 | 1 |
| α-helix | 239-253 | 15 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 88-93 | 6 | |
| α-helix | 104-105 | 2 | |
| α-helix | 110-112 | 3 | |
| α-helix | 132-149 | 18 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Structural maintenance of chromosomes protein 5 | A | protein | 1069 | Saccharomyces cerevisiae S288C | Q08204 (AlphaFold model) |
| Structural maintenance of chromosomes protein 6 | B | protein | 1114 | Saccharomyces cerevisiae S288C | Q12749 (AlphaFold model) |
| E3 SUMO-protein ligase MMS21 | C | protein | 251 | Saccharomyces cerevisiae S288C | P38632 (AlphaFold model) |
| DNA repair protein KRE29 | D | protein | 64 | Saccharomyces cerevisiae S288C | P40026 (AlphaFold model) |
>8I4V_1 Structural maintenance of chromosomes protein 5 (chains A) KRVKIAKPDLSSFQPGSIIKIRLQDFVTYTLTEFNLSPSLNMIIGPNGSGKSTFVCAVCL GLAGKPEYIGRSKKVEDFIKNGQDVSKIEITLKNSPNVTDIEYIDARDETIKITRIITRS KRRSDYLINDYQVSESVVKTLVAQLNIQLDNLCQFLSQERVEEFARLKSVKLLVETIRSI DASLLDVLDELRELQGNEQSLQKDLDFKKAKIVHLRQESDKLRKSVESLRDFQNKKGEIE LHSQLLPYVKVKDHKEKLNIYKEEYERAKANLRAILKDKKPFANTKKTLENQVEELTEKC SLKTDEFLKAKEKINEIFEKLNTIRDEVIKKKNQNEYYRGRTKKLQATIISTKEDFLRSQ EILAQTHLPEKSVFEDIDIKRKEIINKEGEIRDLISEIDAKANAINHEMRSIQRQAESKT KSLTTTDKIGILNQDQDLKEVRDAVLMVREHPEMKDKILEPPIMTVSAINAQFAAYLAQC VDYNTSKALTVVDSDSYKLFANPILDKFKVNLRELSSADTTPPVPAETVRDLGFEGYLSD FITGDKRVMKMLCQTSKIHTIPVSRRELTPAQIKKLITPRPNGKILFKRIIHGNRLVDIK QSAYGSKQVFPTDVSIKQTNFYQGSIMSNEQKIRIENEIINLKNEYNDRKSTLDALSNQK SGYRHELSELASKNDDINREAHQLNEIRKKYTMRKSTIETLREKLDQLKREARKDVSQKI KDIDDQIQQLLLKQRHLLSKMASSMKSLKNCQKELISTQILQFEAQNMDVSMNDVIGFFN EREADLKSQYEDKKKFVKEARDTPEFQSWMREIRSYDQDTKEKLNKVAEKYEEEGNFNLS FVQDVLDKLESEIAMVNHDESAVTILDQVTAELRELEHTVPQQSKDLETIKAKLKEDHAV LEPKLDDIVSKISARFARLFNNVGSAGAVRLEKPKDYAEWKIEIMVKFRDNAPLKKLDSH TQSGGERAVSTVLYMIALQEFTSAPFRVVDEINQGMDSRNERIVHKAMVENACAENTSQY FLITPKLLTGLHYHEKMRIHCVMAGSWIPNPSEDPKMIHFGETSNYSFD
>8I4V_2 Structural maintenance of chromosomes protein 6 (chains B) MISTTISGKRPIEQVDDELLSLTAQQENEEQQQQRKRRRHQFAPMTQFNSNTLDEDSGFR SSSDVATADQDNFLEESPSGYIKKVILRNFMCHEHFELELGSRLNFIVGNNGSGKSAILT AITIGLGAKASETNRGSSLKDLIREGCYSAKIILHLDNSKYGAYQQGIFGNEIIVERIIK RDGPASFSLRSENGKEISNKKKDIQTVVDYFSVPVSNPMCFLSQDAARSFLTASTSQDKY SHFMKGTLLQEITENLLYASAIHDSAQENMALHLENLKSLKAEYEDAKKLLRELNQTSDL NERKMLLQAKSLWIDVAHNTDACKNLENEISGIQQKVDEVTEKIRNRQEKIERYTSDGTT IEAQIDAKVIYVNEKDSEHQNARELLRDVKSRFEKEKSNQAEAQSNIDQGRKKVDALNKT IAHLEEELTKEMGGDKDQMRQELEQLEKANEKLREVNNSLVVSLQDVKNEERDIQHERES ELRTISRSIQNKKVELQNIAKGNDTFLMNFDRNMDRLLRTIEQRKNEFETPAIGPLGSLV TIRKGFEKWTRSIQRAISSSLNAFVVSNPKDNRLFRDIMRSCGIRSNIPIVTYCLSQFDY SKGRAHGNYPTIVDALEFSKPEIECLFVDLSRIERIVLIEDKNEARNFLQRNPVNVNMAL SLRDRRSGFQLSGGYRLDTVTYQDKIRLKVNSSSDNGTQYLKDLIEQETKELQNIRDRYE EKLSEVRSRLKEIDGRLKSTKNEMRKTNFRMTELKMNVGKVVDTGILNSKINERKNQEQA IASYEAAKEELGLKIEQIAQEAQPIKEQYDSTKLALVEAQDELQQLKEDINSRQSKIQKY KDDTIYYEDKKKVYLENIKKIEVNVAALKEGIQRQIQNACAFCSKERIENVDLPDTQEEI KRELDKVSRMIQKAEKSLGLSQEEVIALFEKCRNKYKEGQKKYMEIDEALNRLHNSLKAR DQNYKNAEKGTCFDADMDFRASLKVRKFSGNLSFIKDTKSLEIYILTTNDEKARNVDTLS GGEKSFSQMALLLATWKPMRSRIIALDEFDVFMDQVNRKIGTTLIVKKLKDIARTQTIII TPQDIGKIADIDSSGVSIHRMRDPERQNNSNFYN
>8I4V_3 E3 SUMO-protein ligase MMS21 (chains C) NDNPIPKSVPLHPKSGKYFHNLHARDLSNIYQQCYKQIDETINQLVDSTSPSTIGIEEQV ADITSTYKLLSTYESESNSFDEHIKDLKKNFKQSSDACPQIDLSTWDKYRTGELTAPKLS ELYLNMPTPEPATMVNNTDTLKILKVLPYIWNDPTCVIPDLQNPADEDDLQIEGGKIELT CPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTTRDCPQAACSQVVSMRDFVRDPIMEL RCKIAKMKESQ
>8I4V_4 DNA repair protein KRE29 (chains D) PILKRTIISKRKAPSNNEDEEIVKTPRKLVNYVPLKIFNLGDSFDDTITTTVAKLQDLKK EILD
Cryo-EM structures of Smc5/6 in multiple states reveal its assembly and functional mechanisms. Li, Q., Zhang, J., Haluska, C. et al. Nat Struct Mol Biol (2024) 31:1532-1542. DOI 10.1038/s41594-024-01319-1 · PubMed
Other PDB entries of the same protein (UniProt Q08204 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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