8IAI: Adducin 1
Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Global map. Determined by electron microscopy at 3.5 Å resolution. Released 3 May 2023.
- Method
- Electron microscopy
- Resolution
- 3.5 Å
- Organism
- Sus scrofa
- Chains
- 31
- Atoms
- 60,300
- Mol. weight
- 3105.12 kDa
- Ligands
- ADP
- Released
- 3 May 2023
Explore 8IAI in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8IAI contains 433 α-helices and 265 β-strands across 31 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 1: 17 helices, 9 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 102-104 | 3 | |
| α-helix | 113-124 | 12 | |
| α-helix | 133-135 | 3 | |
| α-helix | 140-158 | 19 | |
| β-strand | 169-172 | 4 | 1 |
| β-strand | 179-182 | 4 | 1 |
| α-helix | 189-191 | 3 | |
| β-strand | 198-201 | 4 | 1 |
| α-helix | 202-204 | 3 | |
| β-strand | 206-208 | 3 | 1 |
| α-helix | 218-230 | 13 | |
| β-strand | 236-240 | 5 | 1 |
| α-helix | 243-249 | 7 | |
| α-helix | 260-265 | 6 | |
| β-strand | 269-271 | 3 | 1 |
| α-helix | 281-290 | 10 | |
| β-strand | 296-299 | 4 | 1 |
| β-strand | 303-308 | 6 | 1 |
| α-helix | 311-335 | 25 | |
| α-helix | 338-340 | 3 | |
| β-strand | 341 | 1 | 2 |
| α-helix | 368-370 | 3 | |
| α-helix | 374-387 | 14 | |
| α-helix | 402-404 | 3 | |
| α-helix | 496-512 | 17 | |
| α-helix | 517-521 | 5 | |
Chain 2: 14 helices, 9 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 106-109 | 4 | |
| α-helix | 113-124 | 12 | |
| α-helix | 140-159 | 20 | |
| β-strand | 169-172 | 4 | 3 |
| β-strand | 180-182 | 3 | 3 |
| α-helix | 183-184 | 2 | |
| α-helix | 189-191 | 3 | |
| β-strand | 198-200 | 3 | 3 |
| β-strand | 206 | 1 | 3 |
| α-helix | 218-230 | 13 | |
| β-strand | 236-240 | 5 | 3 |
| α-helix | 245-249 | 5 | |
| β-strand | 251 | 1 | 4 |
| α-helix | 260-265 | 6 | |
| β-strand | 268-271 | 4 | 3 |
| α-helix | 279-289 | 11 | |
| β-strand | 296-299 | 4 | 3 |
| β-strand | 304-308 | 5 | 3 |
| α-helix | 311-334 | 24 | |
| α-helix | 346-353 | 8 | |
| α-helix | 369-371 | 3 | |
| α-helix | 374-387 | 14 | |
| α-helix | 393-395 | 3 | |
Chain 3: 18 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 118-121 | 4 | |
| α-helix | 130-147 | 18 | |
| β-strand | 157-160 | 4 | 5 |
| β-strand | 168-170 | 3 | 5 |
| α-helix | 171-172 | 2 | |
| α-helix | 177-179 | 3 | |
| β-strand | 186-188 | 3 | 5 |
| β-strand | 194-196 | 3 | 5 |
| α-helix | 209-216 | 8 | |
| β-strand | 224-228 | 5 | 5 |
| α-helix | 231-238 | 8 | |
| α-helix | 248-253 | 6 | |
| β-strand | 257-260 | 4 | 5 |
| α-helix | 268-278 | 11 | |
| β-strand | 284-288 | 5 | 5 |
| β-strand | 291-296 | 6 | 5 |
| α-helix | 299-322 | 24 | |
| α-helix | 327-329 | 3 | |
| α-helix | 334-337 | 4 | |
| α-helix | 362-375 | 14 | |
| α-helix | 381-383 | 3 | |
| α-helix | 391-393 | 3 | |
| α-helix | 399-402 | 4 | |
| α-helix | 492-508 | 17 | |
| α-helix | 513-515 | 3 | |
| α-helix | 518-523 | 6 | |
Chain 4: 12 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 112-114 | 3 | |
| α-helix | 118-121 | 4 | |
| α-helix | 130-147 | 18 | |
| β-strand | 157-160 | 4 | 6 |
| β-strand | 168-170 | 3 | 6 |
| α-helix | 171-172 | 2 | |
| α-helix | 177-179 | 3 | |
| β-strand | 186-188 | 3 | 6 |
| β-strand | 194-196 | 3 | 6 |
| α-helix | 210-218 | 9 | |
| β-strand | 224-228 | 5 | 6 |
| α-helix | 231-238 | 8 | |
| β-strand | 239 | 1 | 2 |
| α-helix | 248-253 | 6 | |
| β-strand | 256-259 | 4 | 6 |
| α-helix | 268-278 | 11 | |
| β-strand | 284-287 | 4 | 6 |
| β-strand | 291-296 | 6 | 6 |
| α-helix | 299-323 | 25 | |
| β-strand | 329 | 1 | 4 |
| α-helix | 362-375 | 14 | |
| α-helix | 381-383 | 3 | |
Chain 5: 8 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 41-44 | 4 | |
| α-helix | 52-55 | 4 | |
| α-helix | 119-122 | 4 | |
| α-helix | 144-145 | 2 | |
| α-helix | 163-170 | 8 | |
| α-helix | 178-180 | 3 | |
| α-helix | 184-185 | 2 | |
| α-helix | 198-212 | 15 | |
Chain 6: 8 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 41-44 | 4 | |
| α-helix | 119-122 | 4 | |
| α-helix | 125-127 | 3 | |
| α-helix | 144-145 | 2 | |
| α-helix | 163-170 | 8 | |
| α-helix | 178-180 | 3 | |
| α-helix | 184-185 | 2 | |
| α-helix | 198-212 | 15 | |
Chain 7: 7 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 41-44 | 4 | |
| α-helix | 52-55 | 4 | |
| α-helix | 144-145 | 2 | |
| α-helix | 146-148 | 3 | |
| α-helix | 162-170 | 9 | |
| α-helix | 178-180 | 3 | |
| α-helix | 198-212 | 15 | |
Chain 9: 5 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 23-26 | 4 | |
| α-helix | 33-39 | 7 | |
| α-helix | 43-55 | 13 | |
| α-helix | 57-63 | 7 | |
| α-helix | 65-81 | 17 | |
23 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Adducin 1 | 1, 2, 9 | protein | 744 | Sus scrofa | A0A8D1Q0D0 (AlphaFold model) |
| Beta-adducin | 3, 4 | protein | 724 | Sus scrofa | I3L5B0 (AlphaFold model) |
| Dematin actin binding protein | 5, 6, 7 | protein | 405 | Sus scrofa | F1RMC1 (AlphaFold model) |
| Actin, cytoplasmic 1 | A, B, C, D, E, F, G, H, I, J, K | protein | 375 | Sus scrofa | Q6QAQ1 (AlphaFold model) |
| Spectrin beta chain | M, N, O, P, Q, R, S, T | protein | 2148 | Sus scrofa | A0A480J001 |
| Tropomyosin-1.9 | U | protein | 248 | Sus scrofa | A0AA82WPI2 |
| Tropomyosin 3 | V | protein | 248 | Sus scrofa | Q6QA25 |
| Tropomodulin-1 | Y | protein | 359 | Sus scrofa | A0A4X1UDZ5 |
| SH3 domain-binding glutamic acid-rich-like protein | Z | protein | 107 | Sus scrofa | A0A8W4FER1 |
Sequence of entity 1 (1, 2, 9), FASTA
>8IAI_1 Adducin 1 (chains 1, 2, 9)
MNGDTRAAVVTSPPPTTAPHKERYFDRVDENNPEYLRERNMAPDLRQDFNMMEQKKRVSM
ILQSPAFCEELESMIQEQFKKGKNPTGLLALQQIADFMTTNVPNVYPAAPQGGMAALNMS
LGMVTPVNDLRGSDSIAYDKGEKLLRCKLAALYRLADLFGWSHLIYNHITTRVSSEQEHF
LIVPFGLLYSEVTASSLVKINLQGDVVDRGSTNLGVNQAGFTLHSAVYAARPDVKCVVHI
HTPAGAAVSAMKCGLLPISPEALSLGEVAYHDYHGILVDEEEKVLIQKNLGPKSKVLILR
NHGLVSVGESVEEAFYYIHNLVVACEIQVRTLASAGGPDNLVLLDPGKYKAKSRPPTSPA
GEGSGSPPAWQIGEQEFEALMRMLDNLGYRTGYPYRYPALREKSKKYSDVEVPASVTGYS
VASDGDSGTGSPLRHSFQKQQREKTRWLNSGRGDDASEEGQAGSSPKSKTKWTKEDGHRA
SASAVPNLFVPLNTNPKEVQEMRNKIREQNLQDIKTAGPQSQVLCGVVMDRSLVQGELVT
ASKAIIEKEYQPHVVVSTTGPNPFNTLTDRELEEYRREVERKQKGPEETLDERSDQKEDS
PPEPPAAPHTPPSTPVKLEEDLPQEPLSGDDSEAATFRPTLPDLPPDEPSEVLGFPTLEE
EEEEEEVGALCEAGRPPSPARPAAEASPEPAAAQAAEEPASPAAEEGAAADPGSDGSPGK
SPSKKKKKFRTPSFLKKSKKRSDS
Sequence of entity 2 (3, 4), FASTA
>8IAI_2 Beta-adducin (chains 3, 4)
MSEETVPEAASPPPAQGRQYFDRFSEEDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQS
PSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPTSSMNFSMMTPINDLHT
ADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEV
TASSLIKVNILGEVVEKGSSCFPVDTTGFCLHSAIYAARPDVRCIIHLHTPATAAVSAMK
WGLLPVSHNALLVGDMAYFDFNGEMEQEADRINLQKCLGPTCKILVLRNHGVVALGDTVE
EAFYKVFHLQAACEIQVSALSSAGGVENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRL
GEHEFEALMRMLDNLGYRTGYPYRYPLVQEKTKHKSEVEIPATVTAFVFEEDGAPVPALR
QHAQKQQKEKTRWLNTPNTYLRVNVADEVQRSMGSPRPKTTWMKADEVEKSSSGMPIRIE
NPNQFVPLYTDPQEVLDMRNKIREQNRQDVKSAGPQSQLLASVIAEKSRSPSTESQLMSQ
GQADTKDESEETVPNPFSQLTDQELEEYKKEVERKKLELDGEKEPAVEEPGSPGKSAPAS
PAQSPAKSETKSPVGSPSKSVDEEAQKTEPSKPTTEPETTQPEGVVVNGREDEPTAEEIL
SKGLSQMTTHADTDVDTSKDKTESVTSGPMSPEGSPSKSPSKKKKKFRTPSFLKKSKKKE
KVES
Sequence of entity 3 (5, 6, 7), FASTA
>8IAI_3 Dematin actin binding protein (chains 5, 6, 7)
MERLQKQPLTSPGSVSSSRGSSVPGSPSSIVAKMDNQVLGYKDLAAIPKDKAILDIERPD
LMIYEPHFTYSLLEHVELPRSRERSLSPKSTSPPPSPEVWAESRSPGTFPQASAPRTTGT
PRTSLPHFHHPETTRPDSNIYKKPPIYKQREPTGGSPQSKHLIEDLIIESSKFPAAQPPD
PNQPAKIETDYWPCPPSLAVVETEWRKRKASRRGAEEEEEEEDDDSGEEMKALRERQREE
LSKVTSNLGKMILKEEMEKSLPIRRKTRSLPDRTPFHTSLQAGTSKSSSLPAYGRTTLSR
LQSTDFSPSGSETESPGLQNGEGQRGRMDRGTSLPCVLEQKIYPYEMLVVTNKGRTKLPP
GVDRMRLERHLSAEDFSRVFSMSPEEFGKLALWKRNELKKKASLF
Sequence of entity 4 (A, B, C, D, E, F, G, H, I, J, K), FASTA
>8IAI_4 Actin, cytoplasmic 1 (chains A, B, C, D, E, F, G, H, I, J, K)
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS
KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT
QIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDL
AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSY
ELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLS
GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQ
EYDESGPSIVHRKCF
Sequence of entity 5 (M, N, O, P, Q, R, S, T), FASTA
>8IAI_5 Spectrin beta chain (chains M, N, O, P, Q, R, S, T)
MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTF
TKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKAL
QFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALL
LWCQMKTAGYPNVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFDV
AERQLGIIQLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIET
EKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLAGVQQQLQAFSTYRTVEKPPKFQEKG
NLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRSELIRQEKL
EQLARRFDRKAAMRETWLNENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRA
LEDLARELELENYHDQKRITARKDNILRLWNYLQELLQSRRQRLETTLALQQLFQDMLHS
IDWMDEIKAHLLSAEFGKHLLEAEDLLQKHKLMEADIAIQGDKVKAITAATLQFTEETGY
QPCDPQVIRDRVSHLEQCFAELSNTAAGRKAQLEQSKRLWKLFWEMDEAKSWIKEKEQIY
SSLGYGKDLTSVLILQRKHKAFEDELRRLDPHLDQIFQEAEDMVALKQFGYPKNEAWVKE
VSAQWDQLKEVPAQWNQLKELAASRKKNLQDTENFFQFQGDVDDLKAWLQDAHKLLSGED
VGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQDFPERFRDSPDVTNRLQVLRDLY
QQVVAQADLRRQRLQDALDLYTVFGETDACELWMGEKEKWLAQMDIPDTLEDLEVVQHRF
DILDQEMKTLIAQIDGVNVAANSLVESNHPRSTEVKQYQDHLNTRWREFQTMVLARREAV
DSALRVHNYCVDCEETSKWIIDKTKVVESTKDLGQDLAGVMAIQRKLSGLERDVAAIQVR
VGALEQESHRLMESHREQEKDIGERQAYVEELWQGLQQALKGQEALLGKSSQLQAFLQDL
DAFEAWLSTAQKEVASKDMPESLPEAEQLLQQHAALKDDIDRHQENYQHVKASGEKVIHG
QTDPEYLLLGQRLEGLDKGWDALCRMWESRGHTLAQCLGFQEFQKDAKQAEAILSNQEYT
LAHLEPPDSLEAAEAGIRKFQDFFTTMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQ
QIEDRHKRNNEKAQEASVLLQDNLALQNFLQNCQELTLWINDKLLTSQDVSYDDARNLHN
KWLKHTAFEAELASQQGWLENIDAEGKQLMEEKPQFEALVSQRLEALHRLWDELQATTKE
IGQRLSAARSSDLRSQTHADLNKWIRAMEDQLRSDDLGKDLTSVNRMLAKLKRVEDQVNV
RKEELGELFAHMPSPGEEAEDEDLSIEKRFLDLLEPLGRRKKQLESSKAKLQISRDLEDE
TLWVEERLPLAQSTDYGANLQTVQLFMKKNQTLQNEILGHAPRVEDVLYRGHQLVEAAEI
DCQDIEERLGHLQNSWDTLQEAAAGRLQRLWDASEAQQYYLDAGEAEAWISEQELYVISD
EMPQDEEGAIVMLKRHLRQQRMVEEYERNIKQLAGRAQSLLAAGHPEGEQIIRLQGQVDK
HYAGLKDMAEDRKRKLENKYRQFQMEREADDLEQWILEKDLVASSPEMGQDFDHVTLLRD
KFRDFARETGAIGQERVDNMNALIEHLIDAGHEEAASIAERKDGLNEMWADVLELIDTRM
QLLAASYDRHRYFYTGNEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHQLGV
QVQQFQDVATRLQAAYAGEEADSIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFS
MVRDLLSWMETIIRQIETQERPRDVSSVELLMKYHQGIWAEMDTRSKNFSACLELGESLL
QRQHQASDEIREKLQQVVSKKKEIDEKWKARSDRLSMLLEVCQFSRDASVAEAWLIAQEP
YLSSRDFGHTVDSVEKLIKRHEAFEKSTASWEPRFAALEKPTTLELKERQTPERPKEDAG
PQEEEGETAGEAPRGPHRAATERTSPVSSLSHLSSSWESLLPEPAHPY
Sequence of entity 6 (U), FASTA
>8IAI_6 Tropomyosin-1.9 (chains U)
MAGSSSLEAVRRKIRSLQEQADAAEERAGTLQRELDYERKLRETAEADVASLNRRIQLVE
EELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAE
DADRKYEEVARKLVIIESDLERAEERAELSEGQVRQLEEQLRIMDQTLKALMAAEDKYSQ
KEDKYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEEKVAHAKEENLSMHQMLDQ
TLLELNNM
Sequence of entity 7 (V), FASTA
>8IAI_7 Tropomyosin 3 (chains V)
MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVE
EELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAE
EADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQ
KEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDKLKCTKEEHLCTQRMLDQ
TLLDLNEM
Sequence of entity 8 (Y), FASTA
>8IAI_8 Tropomodulin-1 (chains Y)
MSYRRELEKYRDLDEDEILGALTEEELRTLENELDELDPDNALLPAGLRQKDQTTKAPTG
PFKREELLDHLEKQAKEFKDREDLVPYTGEKRGKVWVPKQKPMDPVLETVTLEPELEEAL
ANASDAELCDIAAILGMHTLMSNQQYYQALGSSSIVNKEGLNSVIKPTQYKPVPDEEPNA
TDVEETLERIKNNDPKLEEVNLNNIRNIPIPTLKAYAEALKENSYVKKFSIVGTRSNDPV
AFALAEMLKVNKVLKTLNVESNFISGAGILRLVEALPYNTSLVELKIDNQSQPLGNKVEM
EIVSMLEKNATLLKFGYHFTQQGPRLRASNAMMNNNDLVRKRRLADLTGPIIPKCRSGI
Sequence of entity 9 (Z), FASTA
>8IAI_9 SH3 domain-binding glutamic acid-rich-like protein (chains Z)
MVIRVFIASSSGFVAIKKKQQDVVRFLEANKIEFEEVDITMSEEQRQWMYKNIPPEKKPA
QGNPLPPQIFNGDRYCGDYDSFFESKESNTVFSFLGLKSQLASKAEP
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ADP | Adenosine-5'-diphosphate | C10 H15 N5 O10 P2 | 11 |
Primary citation
Structural basis of membrane skeleton organization in red blood cells. Li, N., Chen, S., Xu, K. et al. Cell (2023) 186:1912-1929.e18. DOI 10.1016/j.cell.2023.03.017 · PubMed
Other PDB entries of the same protein (UniProt A0A8D1Q0D0 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8IAH 3.6 Å, Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I,…
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