8INR: PDB entry 8INR

Cryo-EM structure of the alpha-MSH-bound human melanocortin receptor 5 (MC5R)-Gs complex. Determined by electron microscopy at 2.73 Å resolution. Released 20 Sept 2023.

Method: Electron microscopy
Resolution: 2.73 Å
Organisms: Influenza A virus (A/Victoria/3/1975(H3N2)), Homo sapiens, synthetic construct
Chains: 5
Atoms: 7,219
Mol. weight: 148.56 kDa
Ligands: CA
Released: 20 Sept 2023

Explore 8INR in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8INR contains 29 α-helices and 36 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	14-38	25
β-strand	40-46	7	1
α-helix	53-59	7
β-strand	208-214	7	1
β-strand	217-223	7	1
α-helix	234-238	5
β-strand	243-249	7	1
α-helix	266-278	13
β-strand	286-292	7	1
α-helix	294-303	10
α-helix	308-310	3
α-helix	315-317	3
α-helix	326-327	2
α-helix	332-351	20
β-strand	359-362	4	1
α-helix	371-390	20

Chain B: 4 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	5-24	20
α-helix	32-34	3
α-helix	38-39	2
β-strand	45-52	8	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
α-helix	95	1
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-153	8	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	178-181	4	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	217-222	6	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	260-264	5	7
β-strand	273-278	6	8
β-strand	285-289	5	8
β-strand	294-297	4	8
β-strand	304-308	5	8
β-strand	315	1	9
β-strand	319-320	2	2
β-strand	327-330	4	2
β-strand	331	1	9
β-strand	335-341	7	2

Chain G: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	11-23	13
α-helix	30-33	4
α-helix	41-43	3
α-helix	53-55	3
α-helix	56-58	3

Chain R: 10 helices, 0 β-strands

Element	Residues	Length
α-helix	40-62	23
α-helix	70-99	30
α-helix	108-145	38
α-helix	147-152	6
α-helix	156-179	24
α-helix	184-220	37
α-helix	233-263	31
α-helix	268-274	7
α-helix	277-295	19
α-helix	300-310	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
HA signal peptide,Melanocortin receptor 5,LgBiT subunit	R	protein	513	Influenza A virus (A/Victoria/3/1975(H3N2)), Homo sapiens	P03435, P33032 (AlphaFold model)
alpha-melanocyte-stimulating hormone	L	protein	13	synthetic construct	P01189 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s)…	A	protein	361	Homo sapiens	P63092 (AlphaFold model), P63096
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,HiBiT	B	protein	371	Homo sapiens	P62873
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212

Sequence of entity 1 (R), FASTA

>8INR_1 HA signal peptide,Melanocortin receptor 5,LgBiT subunit (chains R)
MKTIIALSYIFCLVFANSSFHLHFLDLNLNATEGNLSGPNVKNKSSPCEDMGIAVEVFLT
LGVISLLENILVIGAIVKNKNLHSPMYFFVCSLAVADMLVSMSSAWETITIYLLNNKHLV
IADAFVRHIDNVFDSMICISVVASMCSLLAIAVDRYVTIFYALRYHHIMTARRSGAIIAG
IWAFCTGCGIVFILYSESTYVILCLISMFFAMLFLLVSLYIHMFLLARTHVKRIAALPGA
SSARQRTSMQGAVTVTMLLGVFTVCWAPFFLHLTLMLSCPQNLYCSRFMSHFNMYLILIM
CNSVMDPLIYAFRSQEMRKTFKEIICCRGFRIACSFPRRDGSSGGGGSGGGGSSGVFTLE
DFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEG
LSADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKK
ITVTGTLWNGNKIIDERLITPDGSMLFRVTINS

Sequence of entity 2 (L), FASTA

>8INR_2 alpha-melanocyte-stimulating hormone (chains L)
SYSMEHFRWGKPV

Sequence of entity 3 (A), FASTA

>8INR_3 Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 4 (B), FASTA

>8INR_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,HiBiT (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSG
VSGWRLFKKIS

Sequence of entity 5 (G), FASTA

>8INR_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	1

Primary citation

Structural insights into ligand recognition and subtype selectivity of the human melanocortin-3 and melanocortin-5 receptors. Feng, W., Zhou, Q., Chen, X. et al. Cell Discov (2023) 9:81. DOI 10.1107/S0907444909042073 · PubMed

Other PDB entries of the same protein (UniProt P03435), best resolution first:

6R0E 1.91 Å, Structure of F11TCR in complex with DR1 MHC Class II presenting PKYVKQNTLKLAT
8IOD 2.59 Å, Cryo-EM structure of the PG-901-bound human melanocortin receptor 5 (MC5R)-Gs complex
4O58 2.75 Å, Crystal structure of broadly neutralizing antibody F045-092 in complex with…
8IOC 2.86 Å, Cryo-EM structure of the gamma-MSH-bound human melanocortin receptor 3 (MC3R)-Gs complex
4GMS 2.95 Å, Crystal structure of heterosubtypic Fab S139/1 in complex with influenza A H3…
8JRV 3.3 Å, Cryo-EM structure of the glucagon receptor bound to glucagon and beta-arrestin 1
8JRU 3.5 Å, Cryo-EM structure of the glucagon receptor bound to beta-arrestin 1 in ligand-free state

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