Cryo-EM structure of the 48-nm repeat doublet microtubule from mouse sperm. Determined by electron microscopy at 3.5 Å resolution. Released 5 Jul 2023.
Explore 8IYJ in 3D Show helices and sheets RCSB PDB PDBe
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Cilia- and flagella-associated protein 95 | 0, 7 | protein | 228 | Mus musculus | Q9CQC3 (AlphaFold model) |
| EF-hand domain-containing family member B | 1, 2 | protein | 853 | Mus musculus | Q8CDU5 (AlphaFold model) |
| Cilia- and flagella-associated protein 53 | 3, 4 | protein | 514 | Mus musculus | Q9D439 (AlphaFold model) |
| Nucleoside diphosphate kinase 7 | 5, 6, j1 | protein | 395 | Mus musculus | Q9QXL8 (AlphaFold model) |
| Cilia- and flagella-associated protein 107 | 8 | protein | 196 | Mus musculus | Q4KKZ1 |
| Cilia- and flagella-associated protein 141 | A, N1 | protein | 101 | Mus musculus | Q9D9D9 |
| Tektin-1 | A0, A1, A2, A3, A4, A5 | protein | 418 | Mus musculus | Q9DAJ2 |
| Tubulin alpha-3 chain | AA, AC, AE, AG, AI, AK, AM, AO, BC, BE, BG, BI, BK, BM, BO, CC, CE, CG, CI, CK, CM, CO, CQ, DC, DE, DG, DI, DK, DM, DO, DQ, EA, EC, EE, EG, EI, EK, EM, EO, FA, FC, FE, FG, FI, FK, FM, FO, GA, GC, GE, GG, GI, GK, GM, GO, HA, HC, HE, HG, HI, HK, HM, HO, IA, IC, IE, IG, II, IK, IM, IO, JC, JE, JG, JI, JK, JM, JO, JQ, KA, KC, KE, KG, KI, KK, KM, KO, LA, LC, LE, LG, LI, LK, LM, LO, MA, MC, ME, MG, MI, MK, MM, MO, NA, NC, NE, NG, NI, NK, NM, NO, OA, OC, OE, OG, OI, OK, OM, OO, PC, PE, PG, PI, PK, PM, PO, QC, QE, QG, QI, QK, QM, QO, RC, RE, RG, RI, RK, RM, RO, SA, SC, SE, SG, SI, SK, SM, TA, TC, TE, TG, TI, TK, TM, UA, UC, UE, UG, UI, UK, UM, UO, VA, VC, VE, VG, VI, VK, VM, VO, WA, WC, WE, WG, WI, WK, WM, WO | protein | 450 | Mus musculus | P05214 |
| Tubulin beta-4B chain | AB, AD, AF, AH, AJ, AL, AN, AP, BB, BD, BF, BH, BJ, BL, BN, BP, CB, CD, CF, CH, CJ, CL, CN, CP, DB, DD, DF, DH, DJ, DL, DN, DP, EB, ED, EF, EH, EJ, EL, EN, FB, FD, FF, FH, FJ, FL, FN, GB, GD, GF, GH, GJ, GL, GN, HB, HD, HF, HH, HJ, HL, HN, HP, IB, ID, IF, IH, IJ, IL, IN, IP, JB, JD, JF, JH, JJ, JL, JN, JP, KB, KD, KF, KH, KJ, KL, KN, KP, LB, LD, LF, LH, LJ, LL, LN, LP, MB, MD, MF, MH, MJ, ML, MN, MP, NB, ND, NF, NH, NJ, NL, NN, NP, OB, OD, OF, OH, OJ, OL, ON, OP, PB, PD, PF, PH, PJ, PL, PN, PP, QB, QD, QF, QH, QJ, QL, QN, QP, RD, RF, RH, RJ, RL, RN, RP, SB, SD, SF, SH, SJ, SL, SN, TB, TD, TF, TH, TJ, TL, TN, UB, UD, UF, UH, UJ, UL, UN, VB, VD, VF, VH, VJ, VL, VN, WB, WD, WF, WH, WJ, WL, WN, WP | protein | 445 | Mus musculus | P68372 |
| Meiosis-specific nuclear structural protein 1 | B, C | protein | 491 | Mus musculus | Q61884 |
| Tektin-2 | B0, B1, B2, B3, B4, B5, R0, R1, R2, R3, R4, R5 | protein | 430 | Mus musculus | Q922G7 |
| Tektin-3 | C0, C1, C2, C3, C4, C5, C6, S0, S1, S2, S3, S4, S5, S6, S7, S8, S9 | protein | 490 | Mus musculus | Q6X6Z7 |
37 more molecules are not listed.
>8IYJ_1 Cilia- and flagella-associated protein 95 (chains 0, 7) MGTFDSQCHECFESKERWYEIGPTDLLERKGSLTLRSHHKKYSEPVLVYSWHRNREAFPK DYDIESLETVKNLCMSTYKRFGSDSPRWMSEAREQMAQVLVNKDLVEKKKTGLLDEETLC PGILERDPELPPTGFRDVPMESPPDPRKPCFSTTYSEDFTPQYEYPPPACPRREEYSIVH RKCRSQFTDLNGSKRLGINTWHDESGIYANSEAKQKLYALARNPIVPF
>8IYJ_2 EF-hand domain-containing family member B (chains 1, 2) MCSFVRVGSPKPLQTSASPLEMSSLRRTRAPEISELGLTPEQKDDIRDRVLRGSKSPTEL GFDLRLEQDRKWRERMGSSEAKSPPCHALGVGLERHTISGTPTEMGNLGLHKGSAFQGSK PLGVLPGRVGPENKGLPPRLRYGGTLHPPFSTVHASPLAAESRRRPLAWGSAWTDAVVEK QPVVGLELRKEPEKEPTCVVMNPYPEMPPKEVDIGLPQTQESDEAKNTEPLIGLVREPSE CPFAQQPEEKKEPGSTEPGVEPPGNIRPIYSGKFFDRVPCWPSAGKVKPVGYRVATCLTE KLPRLMTPPEAKKYFNFRYPPAGAERVFYGRANDPQIAPYLTHGLRSKISIPMGSLINPQ PITTFQQKIKDKKESIYFSHQRAPLGKSHDQTPGLPKGMDVINTTLGTPTIRELSVRDTV NPSKSFEDVLKEGQEGHDLYTVSHNDYFAGEAKNRKYNPASFHRFNLYGIPTPHFNDGRT MAKALHWLHELQMERGAKIVSKRVDDFKEKFQHKLGKVLDPIAETMNVPPGHTFGSCLHP EEYGAGDLIHYRSPDEYLRGKDHQRAVVAAARHHLKKFNHQNFDTLQVAFRHYDKKGDGV IDRAELHEACVQANLHLDKMLLDHLFDYCDVDQDGLINYLEFANFLNWKDRIPLKEHEKR VVVKGKKPDCENVTDTSMGEAEPSLLINPEDIVPKEPGSSEETLRTIQRPGDKVSHQYKT TSSEINAVVGAVPSMCHPIFGVPTIRSDISAPRIRRVSDMNNYGDEGNAYSLLHPSIFSQ KGVFERDFFKTRSKEEISDILTNIGVKLSKEEFENVWNLASKKHQRGEVCVETIRNVLDE LLHADLVKCKTAM
>8IYJ_3 Cilia- and flagella-associated protein 53 (chains 3, 4) MYSQQFGTVPREFKGPTPKAVIIRAKPPKAQRAEQHLKRIQRSYHKYHTTLASIKSNEEN RLKCDWIQRNNHKTFDSLVQARVQDAMQGFVINTEERRNKLRELLASEENEYFSEMQLKG ETIEEKKDKMRERTKLLREKKEKERQEFVAEKLDQQFRERCEELRTKLASIHEKKVVEER NAQIEFNKELKRQKLVEEHLFARLWEEDRLAKERREAQEEKRQRELVQNTRLGLDAQVTS IQAQRQGARRMKEEEARILEQNKAQIKREDEQEKLQKQKRRQETRSSLKKAVQDKIESMQ REYREDLDLNMKLVGRALQDLQDEADKKKQKREEMGREQKIYNDYLMQRREEEKAQEKEL NRLLEDIKAKKLAEKDRELALQRAARKQLMNEVMNTRKLQVQERLQRKLREQEELALHEQ RISESLKVLHQEDMEDFARRCALAEEYRNQLQMQIAHQQQAREAEKEEERQEFEAGLAAN KACLDKIQRILSENQALSQNVHPMRRGYPDKPPL
>8IYJ_4 Nucleoside diphosphate kinase 7 (chains 5, 6, j1) MRACQQGRSSSLVSPYMAPKNQSERFAFIAEWYDPNASLLRRYELLFYPVDGSVEMHDVK NRRTFLKRTKYEDLRLEDLFIGNKVNVFSRQLVLIDYGDQYTARQLGSRKEKTLALIKPD AVSKAGEIIEMINKSGFTITKLRMMTLTRKEAADFHVDHHSRPFYNELIQFITSGPVIAM EILRDDAICEWKRLLGPANSGLSRTDAPGSIRALFGTDGVRNAAHGPDTFASAAREMELF FPSSGGCGPANTAKFTNCTCCIIKPHAISEGMLGKNLIAIRDACFGMSAIQMFNLDRANV EEFYEVYKGVVSEYNDMVTELCSGPCVAIEIQQSNPTKTFREFCGPADPEIARHLRPETL RAIFGKTKVQNAVHCTDLPEDGLLEVQYFFKILDN
>8IYJ_5 Cilia- and flagella-associated protein 107 (chains 8) MAMLSTSVVPEAFSTPGWQIEKKYSTKVLLGNWVEERGKFTKAIDHTPQCIYRKEYVPMP DHRPDFVSRWYSKSKMEGLPYKHLITHHQEPSHRYLISTYDDHYNRHNYNPGLPALRTWN GQKLLWLPEKSDFPLVAPPTNYGLLEQLQQKWLASKTSLKESIYTTSYPRLPVCAMSRRE HAIPVPHPRLQPIPRF
>8IYJ_6 Cilia- and flagella-associated protein 141 (chains A, N1) MSAEKMTKLEENLQRAVALKKTVDRWRNFHIHCMWQTTLDQRRNLFAALRMKDTKEQELA LSNKQLLVVRQAALHELFEKEYQQYQQELNQMGKAFYEERL
>8IYJ_7 Tektin-1 (chains A0, A1, A2, A3, A4, A5) MAKLLQSPPRFLPEEWYIANKSQYHRAEAQRSQSERLVAESQRLVEEIEKTTRKSQSDVN KKLEQRLEEVRFWKKELDDKLEQLVNQTDDLLTYKTRLERSLESYKEPLHITEKCLEYRE KRVGIDLVHDVVEQELQKEADIIHGVMNLLIRTLEESTEQIRLNRSAKYNLEKDLRDKFT AITIDDVCFSLNNNSPNINFSEKVVRIEPNSVSLEDWLDFSNANVEKADKQLNNSTALKT LVDQILSQTANDLRRQCEVVDEAFINGLKETKDARNKLADHLAKVMEEIASQEKNIMALE NAITQQEGPAKVAHTRLETRTHRPNVELCRDIAQYRLIKEIQEINHNVARLKETLAQAQT QLKALYRRQLALQEEIQVKENTIYIDQVLCMEMRKSIPPRDGDDHGAWEGGIRAEAIC
>8IYJ_8 Tubulin alpha-3 chain (chains AA, AC, AE, AG, AI, AK, AM, AO, BC, BE, BG, BI, BK, BM, BO, CC, CE, CG, CI, CK, CM, CO, CQ, DC, DE, DG, DI, DK, DM, DO, DQ, EA, EC, EE, EG, EI, EK, EM, EO, FA, FC, FE, FG, FI, FK, FM, FO, GA, GC, GE, GG, GI, GK, GM, GO, HA, HC, HE, HG, HI, HK, HM, HO, IA, IC, IE, IG, II, IK, IM, IO, JC, JE, JG, JI, JK, JM, JO, JQ, KA, KC, KE, KG, KI, KK, KM, KO, LA, LC, LE, LG, LI, LK, LM, LO, MA, MC, ME, MG, MI, MK, MM, MO, NA, NC, NE, NG, NI, NK, NM, NO, OA, OC, OE, OG, OI, OK, OM, OO, PC, PE, PG, PI, PK, PM, PO, QC, QE, QG, QI, QK, QM, QO, RC, RE, RG, RI, RK, RM, RO, SA, SC, SE, SG, SI, SK, SM, TA, TC, TE, TG, TI, TK, TM, UA, UC, UE, UG, UI, UK, UM, UO, VA, VC, VE, VG, VI, VK, VM, VO, WA, WC, WE, WG, WI, WK, WM, WO) MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD RIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTA VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA SLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN QMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE AREDLAALEKDYEEVGVDSVEAEAEEGEEY
>8IYJ_9 Tubulin beta-4B chain (chains AB, AD, AF, AH, AJ, AL, AN, AP, BB, BD, BF, BH, BJ, BL, BN, BP, CB, CD, CF, CH, CJ, CL, CN, CP, DB, DD, DF, DH, DJ, DL, DN, DP, EB, ED, EF, EH, EJ, EL, EN, FB, FD, FF, FH, FJ, FL, FN, GB, GD, GF, GH, GJ, GL, GN, HB, HD, HF, HH, HJ, HL, HN, HP, IB, ID, IF, IH, IJ, IL, IN, IP, JB, JD, JF, JH, JJ, JL, JN, JP, KB, KD, KF, KH, KJ, KL, KN, KP, LB, LD, LF, LH, LJ, LL, LN, LP, MB, MD, MF, MH, MJ, ML, MN, MP, NB, ND, NF, NH, NJ, NL, NN, NP, OB, OD, OF, OH, OJ, OL, ON, OP, PB, PD, PF, PH, PJ, PL, PN, PP, QB, QD, QF, QH, QJ, QL, QN, QP, RD, RF, RH, RJ, RL, RN, RP, SB, SD, SF, SH, SJ, SL, SN, TB, TD, TF, TH, TJ, TL, TN, UB, UD, UF, UH, UJ, UL, UN, VB, VD, VF, VH, VJ, VL, VN, WB, WD, WF, WH, WJ, WL, WN, WP) MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYV PRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV RKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVV EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCL RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMM AACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRG LKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS EYQQYQDATAEEEGEFEEEAEEEVA
>8IYJ_10 Meiosis-specific nuclear structural protein 1 (chains B, C) MATKKRALSFSEKHQKLVDEKFRKSLNIQVMNKLERQAKNQVVQNENDEKVERQRFLRVL QNEQFELDMEEAIQKAEANKMLRDRQLEQEERLANELARLKHESLKDKKMRQQVRENSIE LRELEQKLKAAYMNKERAAQIVEKDAMKYEQMKRDAEIERIMMEEHDRLLKEESAKQERR NKERAQYYLDLEKQLEDQERRKQEAYEQLLKEKLMIDEIVRKIYEEDQVERQQKLEKKNA IQKYIEEFQRAQDFWRQKKREEMEEENRKIIEFANIQEQREGERMARVHEIEEKRVQRQN LLMKQLEETLRQRDDLEQVRQELYQEEQAEIIKLKVKEEAELRLRRQREMKQDFEDQMAL KELILQAAKEEEETFKKAMLAKFAEDDRIELMNAQKQRMKQLEHKRAVEKLIEERRSQFL ADKQRELEELQLQQRRQGCINEIIEEERLRLLKEHAAKLLGYLPKGVFKREDDVDMLGEE FRKAYQKRDGV
>8IYJ_11 Tektin-2 (chains B0, B1, B2, B3, B4, B5, R0, R1, R2, R3, R4, R5) MATLSFKPSERYRLSDWRTNSYLLSTNAERQRDASHQIRQEARILRNETNNQIVWDEHDN RTRLAERIDTVNRWKETLDKCLTDLDAEIDSLAQAKESAEQNLQAKNLPLDVAIECLTLR ESRRDIDVVRDPVEEELLKEVEVIEATKKVLQEKISQAFQHLCLLQEIRQQLNSDHRDKM ETLEIDRGCLSLNLTSPNISLKVNPTRIPKDSTTLQQWDEFTRFNKNRAEAEMKASIELR EAIALAIAQTNNELDAQRVATEFTFRKRLREMESFYSELKWQEKNTLEEIAELQGDIRRL EEDLRRKMMNLKLAHTRLESRTYRSNVELCRDQTQYGLIDEVHQLEATINTMKQKLAQTQ NALDALFKHLARIQADIACKTNTLLLDTKCMDTRRKLTVPAEKFVPQVDTFTRTTNRTLS PLKICQLELT
>8IYJ_12 Tektin-3 (chains C0, C1, C2, C3, C4, C5, C6, S0, S1, S2, S3, S4, S5, S6, S7, S8, S9) MELLGSTLTATYAHPPPASASFLPAIGTITSSYKDRFPHRNLTHSLSLPWRPNTYYKTAY NYPTLAPYSSRSQRVCESTMLPFVSNRTTFFTRYTPDDWYRSNLVSFQESNSSRHNSERL RVDTSRLIQDKYQQIRKTQAHSTQNLGERVNDLAFWKSEITHELDEMIGETNALTDIKRR LERGLIETEGPLQVSRECLFHREKRMGIDLVHDEAEKELLAEVDTILCCQERMRQHLDKA NAQLASDRSAQHELEKDLSDKQAALRIDDKCQHLRNTSEGVSYFRGVERVDATVSVPETW AKFTDDNVLRSQSERAASAKLREETENLLIVTANEMWNQFNKVNLAFTNRIAETVDAKNK IHTHLTKTLQEIFQIEMTIESIKKAIKEKSAFLKVAQTRLDERTRRPNVELCRDMAQLRL VNEVYEVDETIQTLQQRLRDSEDTLQSLAHTKATLEHDLAVKANTLYIDQEKCMSMRNSY PSTLRLVGYC
| ID | Name | Formula | Copies |
|---|---|---|---|
| GDP | Guanosine-5'-diphosphate | C10 H15 N5 O11 P2 | 176 |
| MG | Magnesium ion | Mg | 178 |
| GTP | Guanosine-5'-triphosphate | C10 H16 N5 O14 P3 | 178 |
Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility. Zhou, L., Liu, H., Liu, S. et al. Cell (2023) 186:2897-2910.e19. DOI 10.1016/j.cell.2023.05.009 · PubMed
Other PDB entries of the same protein (UniProt Q9CQC3 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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