8JNA: CRAF ras-binding domain chimera, apo form

CRAF ras-binding domain chimera, apo form. Determined by X-ray diffraction at 1.7 Å resolution. Released 19 Jun 2024.

Method: X-ray diffraction
Resolution: 1.7 Å
Organism: Homo sapiens
Chains: 1
Atoms: 633
Mol. weight: 10.52 kDa
Released: 19 Jun 2024

Explore 8JNA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8JNA contains 4 α-helices and 7 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 4 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	57-62	6	1
β-strand	66-71	6	1
β-strand	77	1	2
α-helix	78-88	11
α-helix	93-95	3
β-strand	96-101	6	1
β-strand	109-112	4	1
β-strand	117	1	2
α-helix	118-121	4
β-strand	125-130	6	1
α-helix	131-132	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
RAF proto-oncogene serine/threonine-protein kinase, CRaf	B	protein	94	Homo sapiens	P04049 (AlphaFold model), P15056 (AlphaFold model)

Sequence of entity 1 (B), FASTA

>8JNA_1 RAF proto-oncogene serine/threonine-protein kinase, CRaf (chains B)
GPLGSDPSKTSNTIRVFLPNSQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVYRLQDG
EKKPIGWNTDAAWLIGEELQVEFLDHVPLTTHNF

Primary citation

Small-molecule RAS/RAF-binding Inhibitors Allosterically Disrupt RAF Conformation and Exert Efficacy Against Broad-spectrum RAS-driven Cancers. Yoshikawa, Y., Makino, Y., Kubota, H. et al. To be published.

Other PDB entries of the same protein (UniProt P04049 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3IQU 1.05 Å, Crystal Structure of human 14-3-3 sigma in Complex with Raf1 peptide (6mer)
3IQJ 1.15 Å, Crystal Structure of human 14-3-3 sigma in Complex with Raf1 peptide (10mer)
3IQV 1.2 Å, Crystal Structure of human 14-3-3 sigma in Complex with Raf1 peptide (6mer) and…
6VJJ 1.4 Å, Crystal Structure of wild-type KRAS4b (GMPPNP-bound) in complex with RAS-binding domain…
8ATS 1.4 Å, Small molecular stabilizer for C-RAF (pS259) and 14-3-3 (1075306)
9EW1 1.4 Å, Ternary structure of 14-3-3s, CRAF phosphopeptide (pS259) and compound 79 (1124379).
9EW3 1.4 Å, Ternary structure of 14-3-3s, C-RAF phosphopeptide 12-mer (pS259) and compound 78…
8AV0 1.5 Å, small molecule stabilizer (compound 1) for C-RAF pS259 and 14-3-3
9EW5 1.5 Å, Ternary structure of 14-3-3s, C-RAF phosphopeptide (pS259) 12mer and compound 23 (1083848)
9S2J 1.5 Å, Ternary structure of 14-3-3s, CRAF S257L NS mutant phosphopeptide (pS259) and compound…
9S2P 1.5 Å, Ternary structure of 14-3-3s, CRAF V263A NS mutant phosphopeptide (pS259), and compound…
8A68 1.6 Å, Small molecule stabilizer (compound 5) for C-RAF(pS259) and 14-3-3

Browse structure collections

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