8K8C: C/EBPalpha BZIP domain

Crystal structure of C/EBPalpha BZIP domain bound to a high affinity DNA. Determined by X-ray diffraction at 2.06 Å resolution. Released 6 Mar 2024.

Method: X-ray diffraction
Resolution: 2.06 Å
Organisms: Homo sapiens, synthetic construct
Chains: 4
Atoms: 1,598
Mol. weight: 22.81 kDa
Released: 6 Mar 2024

Explore 8K8C in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8K8C contains 2 α-helices and 0 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	284-338	55

Chain B: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	284-337	54

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
CCAAT/enhancer-binding protein alpha	A, B	protein	61	Homo sapiens	P49715 (AlphaFold model)
DNA (5'-d(cpaptptpapcpgptpapaptpgp*a)-3')	C	DNA	13	synthetic construct
DNA (5'-d(cpaptptpapcpgptpapaptpgp*t)-3')	D	DNA	13	synthetic construct

Sequence of entity 1 (A, B), FASTA

>8K8C_1 CCAAT/enhancer-binding protein alpha (chains A, B)
GNSNEYRVRRERNNIAVRKSRDKAKQRNVETQQKVLELTSDNDRLRKRVEQLSRELDTLR
G

Sequence of entity 2 (C), FASTA

>8K8C_2 DNA (5'-D(*CP*AP*TP*TP*AP*CP*GP*TP*AP*AP*TP*GP*A)-3') (chains C)
CATTACGTAATGA

Sequence of entity 3 (D), FASTA

>8K8C_3 DNA (5'-D(*CP*AP*TP*TP*AP*CP*GP*TP*AP*AP*TP*GP*T)-3') (chains D)
CATTACGTAATGT

Primary citation

Structural basis for specific DNA sequence recognition by the transcription factor NFIL3. Chen, S., Lei, M., Liu, K. et al. J Biol Chem (2024) 300:105776-105776. DOI 10.1016/j.jbc.2024.105776 · PubMed

Other PDB entries of the same protein (UniProt P49715 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6DC0 2.8 Å, Tribbles (TRIB1) pseudokinase fused to CCAAT-enhancer binding protein (C/EBPalpha) degron

Browse structure collections

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