8P94: Actin-related protein 3
Cryo-EM structure of cortactin stabilized Arp2/3-complex nucleated actin branches. Determined by electron microscopy at 3.3 Å resolution. Released 3 Jan 2024.
- Method
- Electron microscopy
- Resolution
- 3.3 Å
- Organisms
- Homo sapiens, Sus scrofa, Mus musculus
- Chains
- 30
- Atoms
- 50,658
- Mol. weight
- 781.2 kDa
- Ligands
- ADP, MG
- Released
- 3 Jan 2024
Explore 8P94 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8P94 contains 341 α-helices and 348 β-strands across 29 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chains a, b, c, d, h, i, j, k and l: 0 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3 | 1 | 40 |
Chain A: 29 helices, 21 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-7 | 4 | |
| β-strand | 8-12 | 5 | 1 |
| β-strand | 17-21 | 5 | 1 |
| β-strand | 29-31 | 3 | 1 |
| β-strand | 35-37 | 3 | 2 |
| α-helix | 56-58 | 3 | |
| β-strand | 60-62 | 3 | 2 |
| α-helix | 64-68 | 5 | |
| β-strand | 73-75 | 3 | 2 |
| β-strand | 78-79 | 2 | 3 |
| β-strand | 82-83 | 2 | 3 |
| α-helix | 86-95 | 10 | |
| α-helix | 96-100 | 5 | |
| β-strand | 110-114 | 5 | 1 |
| α-helix | 120-128 | 9 | |
| α-helix | 129-133 | 5 | |
| β-strand | 138-143 | 6 | 1 |
| α-helix | 144-148 | 5 | |
| α-helix | 149-151 | 3 | |
| β-strand | 165-170 | 6 | 4 |
| β-strand | 175-181 | 7 | 4 |
| β-strand | 184-185 | 2 | 4 |
| α-helix | 187-189 | 3 | |
| β-strand | 191-193 | 3 | 4 |
| α-helix | 197-211 | 15 | |
| α-helix | 217-219 | 3 | |
| α-helix | 220-228 | 9 | |
| β-strand | 233 | 1 | 5 |
| α-helix | 238-247 | 10 | |
| α-helix | 249-252 | 4 | |
| β-strand | 254-259 | 6 | 6 |
| β-strand | 266-271 | 6 | 6 |
| α-helix | 273-275 | 3 | |
| α-helix | 279-281 | 3 | |
| α-helix | 285-287 | 3 | |
| α-helix | 296-306 | 11 | |
| α-helix | 309-311 | 3 | |
| α-helix | 312-315 | 4 | |
| β-strand | 319-322 | 4 | 4 |
| α-helix | 324-326 | 3 | |
| β-strand | 329 | 1 | 5 |
| α-helix | 331-354 | 24 | |
| α-helix | 360-364 | 5 | |
| β-strand | 367-368 | 2 | 4 |
| α-helix | 373-375 | 3 | |
| α-helix | 376-384 | 9 | |
| α-helix | 390-393 | 4 | |
| β-strand | 395-396 | 2 | 1 |
| α-helix | 397-403 | 7 | |
| α-helix | 405-408 | 4 | |
Chain B: 21 helices, 20 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 7-11 | 5 | 7 |
| β-strand | 18-22 | 5 | 7 |
| β-strand | 30-32 | 3 | 7 |
| β-strand | 36-39 | 4 | 8 |
| β-strand | 40-42 | 3 | 9 |
| β-strand | 56-57 | 2 | 8 |
| α-helix | 59-63 | 5 | |
| α-helix | 65-67 | 3 | |
| β-strand | 68-71 | 4 | 8 |
| β-strand | 74-75 | 2 | 10 |
| β-strand | 78-79 | 2 | 10 |
| α-helix | 82-92 | 11 | |
| α-helix | 102-104 | 3 | |
| β-strand | 105-111 | 7 | 7 |
| α-helix | 117-129 | 13 | |
| β-strand | 135-140 | 6 | 7 |
| α-helix | 141-149 | 9 | |
| β-strand | 154-159 | 6 | 11 |
| β-strand | 164-170 | 7 | 11 |
| β-strand | 173-174 | 2 | 11 |
| β-strand | 180-182 | 3 | 11 |
| α-helix | 186-199 | 14 | |
| α-helix | 209-219 | 11 | |
| α-helix | 227-236 | 10 | |
| β-strand | 242-245 | 4 | 12 |
| β-strand | 251-254 | 4 | 12 |
| α-helix | 256-259 | 4 | |
| α-helix | 262-265 | 4 | |
| α-helix | 268-271 | 4 | |
| α-helix | 278-287 | 10 | |
| α-helix | 291-297 | 7 | |
| β-strand | 301-304 | 4 | 11 |
| α-helix | 306-308 | 3 | |
| α-helix | 313-328 | 16 | |
| α-helix | 334-337 | 4 | |
| β-strand | 344-345 | 2 | 11 |
| α-helix | 350-352 | 3 | |
| α-helix | 353-365 | 13 | |
| β-strand | 373-374 | 2 | 7 |
| α-helix | 375-381 | 7 | |
| α-helix | 383-388 | 6 | |
Chain C: 4 helices, 32 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 2-5 | 4 | 13 |
| β-strand | 14-16 | 3 | 14 |
| β-strand | 22-25 | 4 | 14 |
| β-strand | 32-38 | 7 | 14 |
| β-strand | 41-48 | 8 | 14 |
| β-strand | 55-61 | 7 | 15 |
| β-strand | 66-71 | 6 | 15 |
| β-strand | 76-82 | 7 | 15 |
| β-strand | 85-91 | 7 | 15 |
| β-strand | 99-104 | 6 | 16 |
| β-strand | 110-115 | 6 | 16 |
| β-strand | 120-126 | 7 | 16 |
| β-strand | 131-137 | 7 | 16 |
| β-strand | 145-150 | 6 | 17 |
| β-strand | 156-161 | 6 | 17 |
| β-strand | 166-170 | 5 | 17 |
| α-helix | 178-182 | 5 | |
| β-strand | 183 | 1 | 18 |
| β-strand | 186 | 1 | 18 |
| β-strand | 194-197 | 4 | 17 |
| β-strand | 205-210 | 6 | 19 |
| β-strand | 216-221 | 6 | 19 |
| β-strand | 225-230 | 6 | 19 |
| β-strand | 236-241 | 6 | 19 |
| β-strand | 247-252 | 6 | 20 |
| β-strand | 254 | 1 | 21 |
| β-strand | 257-262 | 6 | 20 |
| β-strand | 268-273 | 6 | 20 |
| β-strand | 278-284 | 7 | 20 |
| α-helix | 291-293 | 3 | |
| α-helix | 298-306 | 9 | |
| β-strand | 329-336 | 8 | 13 |
| β-strand | 341 | 1 | 21 |
| β-strand | 344-349 | 6 | 13 |
| β-strand | 353-356 | 4 | 13 |
| α-helix | 359-365 | 7 | |
Chain D: 18 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-5 | 2 | |
| α-helix | 9-23 | 15 | |
| α-helix | 26-30 | 5 | |
| β-strand | 33-36 | 4 | 22 |
| α-helix | 38-40 | 3 | |
| β-strand | 42-46 | 5 | 22 |
| α-helix | 48-50 | 3 | |
| β-strand | 54-60 | 7 | 22 |
| α-helix | 64-68 | 5 | |
| α-helix | 74-80 | 7 | |
| α-helix | 81-83 | 3 | |
| β-strand | 84 | 1 | 22 |
| α-helix | 86-88 | 3 | |
| β-strand | 93-98 | 6 | 22 |
| α-helix | 102-103 | 2 | |
| α-helix | 106-113 | 8 | |
| α-helix | 116-123 | 8 | |
| α-helix | 126-133 | 8 | |
| β-strand | 142-144 | 3 | 23 |
| β-strand | 151-156 | 6 | 23 |
| β-strand | 161-167 | 7 | 23 |
| α-helix | 172-183 | 12 | |
| α-helix | 186-188 | 3 | |
| β-strand | 197-201 | 5 | 23 |
| β-strand | 221-227 | 7 | 23 |
| α-helix | 237-277 | 41 | |
| α-helix | 278-280 | 3 | |
| α-helix | 282-284 | 3 | |
| β-strand | 290 | 1 | 24 |
| β-strand | 296 | 1 | 24 |
Chain E: 10 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 15-16 | 2 | 25 |
| β-strand | 19-20 | 2 | 25 |
| β-strand | 24 | 1 | 26 |
| α-helix | 33 | 1 | |
| β-strand | 34 | 1 | 26 |
| α-helix | 35 | 1 | |
| α-helix | 41-52 | 12 | |
| β-strand | 57-59 | 3 | 9 |
| α-helix | 63-82 | 20 | |
| α-helix | 88-100 | 13 | |
| α-helix | 102-104 | 3 | |
| α-helix | 118-120 | 3 | |
| α-helix | 123-148 | 26 | |
| β-strand | 149-150 | 2 | 27 |
| β-strand | 155-156 | 2 | 27 |
| α-helix | 161-163 | 3 | |
| α-helix | 168-170 | 3 | |
Chain F: 6 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-16 | 11 | |
| α-helix | 37-40 | 4 | |
| β-strand | 47 | 1 | 28 |
| β-strand | 51-56 | 6 | 29 |
| β-strand | 59-64 | 6 | 29 |
| β-strand | 66 | 1 | 28 |
| β-strand | 69-75 | 7 | 29 |
| α-helix | 81-96 | 16 | |
| β-strand | 104 | 1 | 29 |
| α-helix | 108-109 | 2 | |
| β-strand | 114-119 | 6 | 29 |
| α-helix | 121-125 | 5 | |
| α-helix | 128-165 | 38 | |
Chain G: 10 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-11 | 3 | |
| α-helix | 15-18 | 4 | |
| α-helix | 29-33 | 5 | |
| α-helix | 41-50 | 10 | |
| α-helix | 53-60 | 8 | |
| α-helix | 71-85 | 15 | |
| α-helix | 93-97 | 5 | |
| α-helix | 102-113 | 12 | |
| α-helix | 123-138 | 16 | |
| α-helix | 140-147 | 8 | |
13 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Actin-related protein 3 | A | protein | 418 | Homo sapiens | P61158 (AlphaFold model) |
| Actin-related protein 2 | B | protein | 394 | Homo sapiens | P61160 (AlphaFold model) |
| Actin-related protein 2/3 complex subunit 1B | C | protein | 372 | Homo sapiens | O15143 (AlphaFold model) |
| Actin-related protein 2/3 complex subunit 2 | D | protein | 300 | Homo sapiens | O15144 (AlphaFold model) |
| Actin-related protein 2/3 complex subunit 3 | E | protein | 178 | Homo sapiens | O15145 |
| Actin-related protein 2/3 complex subunit 4 | F | protein | 168 | Homo sapiens | P59998 |
| Actin-related protein 2/3 complex subunit 5-like protein | G | protein | 153 | Homo sapiens | Q9BPX5 |
| Actin, cytoplasmic 1 | H, J, K, N, O, P, Q, R, S, W | protein | 375 | Sus scrofa | Q6QAQ1 |
| Src substrate cortactin | I | protein | 546 | Mus musculus | Q60598 |
| F-actin-capping protein subunit alpha-1 | U | protein | 286 | Mus musculus | P47753 |
| Isoform 2 of F-actin-capping protein subunit beta | V | protein | 272 | Mus musculus | P47757 |
| Phalloidin | a, b, c, d, h, i, j, k, l, m | protein | 7 | Amanita phalloides | |
Sequence of entity 1 (A), FASTA
>8P94_1 Actin-related protein 3 (chains A)
MAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDF
FIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTP
ENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPV
AEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVK
EFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVV
DEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKP
KPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS
Sequence of entity 2 (B), FASTA
>8P94_2 Actin-related protein 2 (chains B)
MDSQGRKVVVCDNGTGFVKCGYAGSNFPEHIFPALVGRPIIRSTTKVGNIEIKDLMVGDE
ASELRSMLEVNYPMENGIVRNWDDMKHLWDYTFGPEKLNIDTRNCKILLTEPPMNPTKNR
EKIVEVMFETYQFSGVYVAIQAVLTLYAQGLLTGVVVDSGDGVTHICPVYEGFSLPHLTR
RLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRMIKEKLCYVGYNIEQEQKLALETTVL
VESYTLPDGRIIKVGGERFEAPEALFQPHLINVEGVGVAELLFNTIQAADIDTRSEFYKH
IVLSGGSTMYPGLPSRLERELKQLYLERVLKGDVEKLSKFKIRIEDPPRRKHMVFLGGAV
LADIMKDKDNFWMTRQEYQEKGVRVLEKLGVTVR
Sequence of entity 3 (C), FASTA
>8P94_3 Actin-related protein 2/3 complex subunit 1B (chains C)
MAYHSFLVEPISCHAWNKDRTQIAICPNNHEVHIYEKSGAKWTKVHELKEHNGQVTGIDW
APESNRIVTCGTDRNAYVWTLKGRTWKPTLVILRINRAARCVRWAPNENKFAVGSGSRVI
SICYFEQENDWWVCKHIKKPIRSTVLSLDWHPNNVLLAAGSCDFKCRIFSAYIKEVEERP
APTPWGSKMPFGELMFESSSSCGWVHGVCFSASGSRVAWVSHDSTVCLADADKKMAVATL
ASETLPLLALTFITDNSLVAAGHDCFPVLFTYDAAAGMLSFGGRLDVPKQSSQRGLTARE
RFQNLDKKASSEGGTAAGAGLDSLHKNSVSQISVLSGGKAKCSQFCTTGMDGGMSIWDVK
SLESALKDLKIK
Sequence of entity 4 (D), FASTA
>8P94_4 Actin-related protein 2/3 complex subunit 2 (chains D)
MILLEVNNRIIEETLALKFENAAAGNKPEAVEVTFADFDGVLYHISNPNGDKTKVMVSIS
LKFYKELQAHGADELLKRVYGSFLVNPESGYNVSLLYDLENLPASKDSIVHQAGMLKRNC
FASVFEKYFQFQEEGKEGENRAVIHYRDDETMYVESKKDRVTVVFSTVFKDDDDVVIGKV
FMQEFKEGRRASHTAPQVLFSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNT
INLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR
Sequence of entity 5 (E), FASTA
>8P94_5 Actin-related protein 2/3 complex subunit 3 (chains E)
MPAYHSSLMDPDTKLIGNMALLPIRSQFKGPAPRETKDTDIVDEAIYYFKANVFFKNYEI
KNEADRTLIYITLYISECLKKLQKCNSKSQGEKEMYTLGITNFPIPGEPGFPLNAIYAKP
ANKQEDEVMRAYLQQLRQETGLRLCEKVFDPQNDKPSKWWTCFVKRQFMNKSLSGPGQ
Sequence of entity 6 (F), FASTA
>8P94_6 Actin-related protein 2/3 complex subunit 4 (chains F)
MTATLRPYLSAVRATLQAALCLENFSSQVVERHNKPEVEVRSSKELLLQPVTISRNEKEK
VLIEGSINSVRVSIAVKQADEIEKILCHKFMRFMMMRAENFFILRRKPVEGYDISFLITN
FHTEQMYKHKLVDFVIHFMEEIDKEISEMKLSVNARARIVAEEFLKNF
Sequence of entity 7 (G), FASTA
>8P94_7 Actin-related protein 2/3 complex subunit 5-like protein (chains G)
MARNTLSSRFRRVDIDEFDENKFVDEQEEAAAAAAEPGPDPSEVDGLLRQGDMLRAFHAA
LRNSPVNTKNQAVKERAQGVVLKVLTNFKSSEIEQAVQSLDRNGVDLLMKYIYKGFEKPT
ENSSAVLLQWHEKALAVGGLGSIIRVLTARKTV
Sequence of entity 8 (H, J, K, N, O, P, Q, R, S, W), FASTA
>8P94_8 Actin, cytoplasmic 1 (chains H, J, K, N, O, P, Q, R, S, W)
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS
KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT
QIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDL
AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSY
ELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLS
GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQ
EYDESGPSIVHRKCF
Sequence of entity 9 (I), FASTA
>8P94_9 Src substrate cortactin (chains I)
MWKASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRE
NVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDRSAVGHEYQSKLSKHCSQVDSVRG
FGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQG
KTEKHESQKDYSKGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQT
DRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQSERQDSSAVGFDYKERLAKHESQQ
DYAKGFGGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKE
REQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKKQTPPASPSPQPIEDRPPSSPI
YEDAAPFKAEPSYRGSEPEPEYSIEAAGIPEAGSQQGLTYTSEPVYETTEAPGHYQAEDD
TYDGYESDLGITAIALYDYQAAGDDEISFDPDDIITNIEMIDDGWWRGVCKGRYGLFPAN
YVELRQ
Sequence of entity 10 (U), FASTA
>8P94_10 F-actin-capping protein subunit alpha-1 (chains U)
MADFEDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNMD
QFTPVKIEGYDDQVLITEHGDLGNSRFLDPRNQISFKFDHLRKEASDPQPEDVDGGLKSW
RESCDSALRAYVKDHYSNGFCTVYAKTIDGQQTIIACIESHQFQPKNFWNGRWRSEWKFT
ITPPSAQVVGVLKIQVHYYEDGNVQLVSHKDVQDSVTVSNEVQTTKEFIKIIESAENEYQ
TAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA
Sequence of entity 11 (V), FASTA
>8P94_11 Isoform 2 of F-actin-capping protein subunit beta (chains V)
MSDQQLDCALDLMRRLPPQQIEKNLSDLIDLVPSLCEDLLSSVDQPLKIARDKVVGKDYL
LCDYNRDGDSYRSPWSNKYDPPLEDGAMPSARLRKLEVEANNAFDQYRDLYFEGGVSSVY
LWDLDHGFAGVILIKKAGDGSKKIKGCWDSIHVVEVQEKSSGRTAHYKLTSTVMLWLQTN
KSGSGTMNLGGSLTRQMEKDETVSDCSPHIANIGRLVEDMENKIRSTLNEIYFGKTKDIV
NGLRSVQTFADKSKQEALKNDLVEALKRKQQC
Sequence of entity 12 (a, b, c, d, h, i, j, k, l, m), FASTA
>8P94_12 Phalloidin (chains a, b, c, d, h, i, j, k, l, m)
WXATCPA
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ADP | Adenosine-5'-diphosphate | C10 H15 N5 O10 P2 | 12 |
| MG | Magnesium ion | Mg | 12 |
Primary citation
Cortactin stabilizes actin branches by bridging activated Arp2/3 to its nucleated actin filament. Liu, T., Cao, L., Mladenov, M. et al. Nat Struct Mol Biol (2024) 31:801-809. DOI 10.1038/s41594-023-01205-2 · PubMed
Other PDB entries of the same protein (UniProt P61158 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 9I2B 3.0 Å, SPIN90-Arp2/3 nucleated bidirectional actin filaments
- 6UHC 3.9 Å, CryoEM structure of human Arp2/3 complex with bound NPFs
- 6YW6 4.2 Å, Cryo-EM structure of the ARP2/3 1B5CL isoform complex.
- 6YW7 4.5 Å, Cryo-EM structure of the ARP2/3 1A5C isoform complex.
Browse structure collections
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