8PIL: Transcription antitermination protein RfaH

E. coli transcription complex paused at ops site and bound to RfaH and NusA. Determined by electron microscopy at 3.2 Å resolution. Released 24 Apr 2024.

Method
Electron microscopy
Resolution
3.2 Å
Organism
Escherichia coli
Chains
10
Atoms
30,440
Mol. weight
494.54 kDa
Ligands
MG, ZN
Released
24 Apr 2024

Explore 8PIL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8PIL contains 173 α-helices and 234 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain F: 4 helices, 9 β-strands

ElementResiduesLengthSheet
α-helix2-109
α-helix20-3819
β-strand60-61277
α-helix71-733
β-strand92-93277
α-helix104-12724
β-strand137-138278
β-strand141-144479
β-strand149-153579
β-strand159-163579
β-strand181-182278
β-strand183-186480
β-strand195-197380
Chain G: 7 helices, 16 β-strands
ElementResiduesLengthSheet
α-helix9-113
β-strand12-17665
β-strand23-321065
α-helix35-4713
β-strand53-61966
β-strand74167
α-helix78-869
β-strand90-92368
β-strand97-105966
β-strand108-111467
α-helix112-1143
β-strand115166
β-strand121-123368
β-strand129-133567
β-strand139-1491166
β-strand152-153269
α-helix155-1617
α-helix165-1673
β-strand170-172366
β-strand175-176269
β-strand180-1891065
β-strand198-2071065
α-helix213-23321
Chain H: 14 helices, 19 β-strands
ElementResiduesLengthSheet
β-strand15-18470
β-strand24-31870
α-helix35-4915
β-strand52-611071
β-strand74172
α-helix78-8710
β-strand90-92373
β-strand97-105971
β-strand109-111374
α-helix112-1143
β-strand115171
α-helix116-1172
β-strand121-123373
β-strand129-132474
β-strand133172
β-strand139-1501271
β-strand152-153275
α-helix155-1584
α-helix162-1654
β-strand170-172371
β-strand175-176275
β-strand181-1921270
β-strand195-2061270
α-helix213-22614
α-helix229-2324
α-helix251-2544
β-strand256176
α-helix257-2593
α-helix264-2729
β-strand277176
α-helix278-2836
α-helix285-2862
α-helix297-31014
Chain I: 70 helices, 95 β-strands
ElementResiduesLengthSheet
α-helix5-95
α-helix11-122
β-strand13-1425
α-helix19-202
α-helix23-264
α-helix29-3911
α-helix48-569
β-strand59-6026
β-strand66-75106
α-helix76-783
α-helix82-887
β-strand93-105136
β-strand114-128156
α-helix1291
β-strand13017
β-strand136-13837
β-strand141-14557
α-helix1461
β-strand147-15158
α-helix1521
β-strand154-15969
β-strand172-17769
β-strand180110
β-strand184-18859
β-strand194-19859
β-strand204-20529
α-helix207-2126
α-helix217-2248
β-strand227-231511
β-strand239-240211
α-helix243-2464
β-strand250112
β-strand255-257313
β-strand260-263413
β-strand268112
α-helix271-28010
β-strand284-285211
α-helix289-2946
β-strand296114
β-strand297111
β-strand301-302215
β-strand309-311315
β-strand316114
α-helix319-3279
β-strand332-336511
β-strand339116
β-strand343116
α-helix346-3538
α-helix359-37012
α-helix375-3762
α-helix378-38912
β-strand396110
α-helix399-40810
α-helix422-43716
α-helix442-4443
α-helix448-4503
β-strand451-45448
α-helix456-48126
α-helix489-4924
α-helix496-50813
β-strand512-51437
α-helix515-5162
α-helix520-5278
β-strand529-53138
α-helix545-5484
α-helix552-5543
β-strand558117
β-strand572-57438
β-strand575117
β-strand576118
β-strand580-581219
β-strand587-595919
β-strand598-6071019
α-helix608-6136
β-strand616-617219
β-strand623120
α-helix625-6273
β-strand629120
β-strand633-637519
β-strand642-646519
α-helix647-6493
β-strand652-654319
β-strand662118
α-helix663-6664
α-helix671-6733
α-helix676-68611
β-strand689121
β-strand700-70125
α-helix705-7117
β-strand716-717222
β-strand722-727623
β-strand731-736623
α-helix7371
α-helix741-7422
β-strand748-752523
β-strand755-758424
β-strand764-766324
β-strand768-769222
β-strand776-777223
β-strand782-785422
β-strand789-790225
β-strand793-794225
β-strand798-804726
β-strand816-819426
α-helix820-8256
β-strand830-8411227
β-strand842128
β-strand847128
β-strand849-850229
α-helix859-8635
β-strand865130
β-strand870131
β-strand871130
α-helix8721
β-strand876-877227
β-strand882-884331
β-strand886-889429
α-helix896-9016
β-strand913-915329
β-strand918-919231
α-helix920-9212
β-strand926-9351027
α-helix940-9423
α-helix943-98139
α-helix986-9905
α-helix994-9996
α-helix1006-103732
α-helix1038-10414
β-strand1046-10581327
α-helix1059-10602
β-strand1065-1067332
β-strand1073-1075332
β-strand1076-1080526
β-strand1087133
α-helix10921
β-strand1093133
α-helix10941
β-strand1096-1098326
α-helix1102-11065
α-helix1110-113324
α-helix1138-115013
α-helix1161-11633
α-helix1168-11758
β-strand1182-118325
α-helix1192-120110
β-strand1209-1210226
β-strand1213133
α-helix12191
β-strand1220133
α-helix12211
β-strand1225-1232826
β-strand1233-1234232
β-strand1235121
α-helix1240-12423
β-strand1244-1246334
β-strand1251135
β-strand1258135
α-helix1267-12682
β-strand1269-1270236
α-helix1272-12809
α-helix1285-12884
α-helix1289-12968
α-helix1298-130912
α-helix1321-133212
β-strand1335-1340637
Chain J: 69 helices, 81 β-strands
ElementResiduesLengthSheet
β-strand19-24637
α-helix25-262
α-helix27-337
β-strand34-37438
α-helix59-624
β-strand66139
β-strand69139
α-helix78-803
α-helix831
β-strand84140
α-helix851
β-strand91140
α-helix95-995
β-strand102-1121138
α-helix1221
α-helix123-1286
α-helix132-1398
β-strand144-148541
β-strand159-160241
α-helix162-17110
β-strand177-179341
α-helix182-19110
α-helix194-20714
α-helix211-22919
α-helix234-2363
β-strand238-244738
α-helix247-2493
β-strand254142
β-strand260142
α-helix261-2633
α-helix264-28421
α-helix289-30719
α-helix315-3162
β-strand317143
β-strand323143
α-helix3371
α-helix338-3425
β-strand345-346236
β-strand350-354534
β-strand355-357344
β-strand365-369544
α-helix370-3767
α-helix378-38811
α-helix394-40310
α-helix406-41510
β-strand421-425534
α-helix431-4333
β-strand434-437434
β-strand438-442544
β-strand447-449344
α-helix453-4575
β-strand465-469534
α-helix474-48310
β-strand485134
α-helix486-4883
β-strand491145
β-strand498145
α-helix505-5139
β-strand526-527246
α-helix530-5389
β-strand547-5571146
β-strand563-5731146
α-helix574-5796
α-helix589-5913
α-helix598-61114
α-helix615-63521
α-helix645-6473
α-helix650-66920
α-helix675-70228
β-strand706-709447
β-strand712-715447
α-helix721-7277
α-helix734-7418
β-strand745148
β-strand748149
α-helix7531
β-strand754149
α-helix7551
β-strand759148
α-helix769-80335
β-strand809-811350
β-strand820-822351
β-strand825-827352
β-strand830-833452
α-helix835-8384
β-strand842-843253
β-strand844151
α-helix847-8482
β-strand849154
α-helix8501
β-strand856154
β-strand863-864253
α-helix866-8749
β-strand880-882351
α-helix885-8873
β-strand894-895250
α-helix896-8994
β-strand901155
β-strand908155
α-helix915-92410
α-helix925-9273
β-strand949156
β-strand956157
β-strand957-961558
β-strand965-967356
β-strand973-975356
β-strand980-985658
β-strand991-997758
α-helix9981
β-strand1002-1003256
β-strand1011157
β-strand1018-1019256
β-strand1024-1028559
β-strand1033-1038660
β-strand1046-1050559
α-helix10511
β-strand1057-1061559
β-strand1077-1081560
β-strand1087160
α-helix10881
β-strand1089161
α-helix10901
β-strand1096161
β-strand1098-1100360
β-strand1106-1107259
β-strand1114-1115260
β-strand1120-1125659
α-helix1138-11469
α-helix1148-11503
β-strand1156162
β-strand1161-1165563
β-strand1172-1178763
β-strand1186-1191663
β-strand1203-1204263
β-strand1209162
α-helix1214-12163
α-helix1217-12248
α-helix1226-124318
α-helix1250-126011
β-strand1263-1268664
β-strand1279-1281364
α-helix1282-129514
α-helix1297-13004
β-strand1301-1305564
α-helix1309-13146
α-helix1319-13257
α-helix1328-133811
β-strand1341-1343337
α-helix1347-13537
α-helix1361-137414
Chain K: 5 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix7-137
α-helix18-3114
α-helix35-373
α-helix46-5510
α-helix61-8323
Chain P: 4 helices, 14 β-strands
ElementResiduesLengthSheet
β-strand3-971
α-helix14-2310
β-strand27-2931
β-strand32-3432
β-strand47-4932
β-strand54-5961
α-helix66-705
β-strand75-7841
β-strand8013
β-strand8513
α-helix861
β-strand8711
α-helix90-989
β-strand114-11854
β-strand128-13254
β-strand139-14354
β-strand150-15454
β-strand159-16134

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Transcription antitermination protein RfaHPprotein164Escherichia coliP0AFW0 (AlphaFold model)
DNA-directed RNA polymerase subunit betaIprotein1342Escherichia coliP0A8V2 (AlphaFold model)
DNA-directed RNA polymerase subunit beta'Jprotein1416Escherichia coliP0A8T7 (AlphaFold model)
DNA-directed RNA polymerase subunit omegaKprotein91Escherichia coliP0A800 (AlphaFold model)
DNA-directed RNA polymerase subunit alphaG, Hprotein329Escherichia coliP0A7Z4
non-template DNAADNA40Escherichia coli
template DNABDNA40Escherichia coli
RNARRNA17Escherichia coli
Transcription termination/antitermination protein NusAFprotein497Escherichia coliP0AFF6
Sequence of entity 1 (P), FASTA
>8PIL_1 Transcription antitermination protein RfaH (chains P)
GHMQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRTAVSEPLFPNYLFVE
FDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVII
TEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFRKL
Sequence of entity 2 (I), FASTA
>8PIL_2 DNA-directed RNA polymerase subunit beta (chains I)
MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQ
SYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQ
EVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYR
GSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQME
LVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKD
YIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRL
SALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGIL
SKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLS
LGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTR
ERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVT
DEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQV
VSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKR
GGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERG
DVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVS
RDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEK
LLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDL
SEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDE
LKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKIN
PIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAK
LREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLK
LGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLG
GKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
ESFNVLLKEIRSLGINIELEDE
Sequence of entity 3 (J), FASTA
>8PIL_3 DNA-directed RNA polymerase subunit beta' (chains J)
VKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCAR
IFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIELASPTAHIWFLKSL
PSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQILTEEQYLDALEEFGDEFDAKM
GAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMILT
VLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQ
EAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPY
LRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKKMVEREEAVVWDILDEVIREHP
VLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEA
RALMMSTNNILSPANGEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAERLYRSG
LASLHARVKVRITEYEKDANGELVAKTSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKA
ISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAE
VAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFN
SIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGAR
KGLADTALKTANSGYLTRRLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVL
GRVTAEDVLKPGTADILVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYG
RDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKL
SNVKSVVNSSGKLVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANW
DPHTMPVITEVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKI
VDAQGNDVLIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPR
VADLFEARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFE
GERVERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQM
LRKATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEAPA
APQVTAEDASASLAELLNAGLGGSDNELEVHHHHHH
Sequence of entity 4 (K), FASTA
>8PIL_4 DNA-directed RNA polymerase subunit omega (chains K)
MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKTTVIALREIEEGLIN
NQILDVRERQEQQEQEAAELQAVTAIAEGRR
Sequence of entity 5 (G, H), FASTA
>8PIL_5 DNA-directed RNA polymerase subunit alpha (chains G, H)
MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
IDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGD
VEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIHSEEDERPIGRLLVDACYSPV
ERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVDLRDVRQP
EVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSL
TEIKDVLASRGLSLGMRLENWPPASIADE
Sequence of entity 6 (A), FASTA
>8PIL_6 non-template DNA (chains A)
CACCACCACGCGGGCGGTAGCGTGCTTTTTTCGATCTTCC
Sequence of entity 7 (B), FASTA
>8PIL_7 template DNA (chains B)
GGAAGATCGAAAAAAGCACGCTACCGCCCGCGTGGTGGTG
Sequence of entity 8 (R), FASTA
>8PIL_8 RNA (chains R)
UUCUUUGGCGGUAGCGU
Sequence of entity 9 (F), FASTA
>8PIL_9 Transcription termination/antitermination protein NusA (chains F)
GPMNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDT
FRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKV
REAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDR
VRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAV
KTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVV
DEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDT
FTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIA
QAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKA
GALIMAARNICWFGDEA

Ligands and cofactors

IDNameFormulaCopies
MGMagnesium ionMg1
ZNZinc ionZn2

Primary citation

Concerted transformation of a hyper-paused transcription complex and its reinforcing protein. Zuber, P.K., Said, N., Hilal, T. et al. Nat Commun (2024) 15:3040-3040. DOI 10.1038/s41467-024-47368-4 · PubMed

Other PDB entries of the same protein (UniProt P0AFW0 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 8PIL directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.