8QV2: Tubulin gamma chain

Structure of the native y-Tubulin Ring Complex (yTuRC) capping microtubule minus ends at the spindle pole body. Determined by electron microscopy at 9.2 Å resolution. Released 24 Apr 2024.

Method: Electron microscopy
Resolution: 9.2 Å
Organism: Saccharomyces cerevisiae
Chains: 90
Atoms: 255,200
Mol. weight: 5473.44 kDa
Ligands: GTP, GDP
Released: 24 Apr 2024

Explore 8QV2 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8QV2 contains 1,426 α-helices and 949 β-strands across 90 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain a: 15 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	4-10	7	23
α-helix	11-29	19
β-strand	54-56	3	24
β-strand	62-64	3	24
β-strand	66-69	4	23
α-helix	73-82	10
α-helix	89-91	3
β-strand	93	1	23
α-helix	99-100	2
α-helix	105-127	23
β-strand	133-141	9	23
α-helix	145-161	17
β-strand	166-173	8	23
α-helix	180-196	17
β-strand	200-205	6	23
α-helix	206-216	11
α-helix	224-243	20
α-helix	252-257	6
β-strand	267-272	6	23
α-helix	291-297	7
α-helix	301-303	3
β-strand	304	1	23
β-strand	315-324	10	23
α-helix	328-341	14
β-strand	352-354	3	23
β-strand	372-380	9	23
α-helix	384-394	11
α-helix	416-441	26

Chain Ab: 17 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	8
α-helix	11-27	17
β-strand	54-56	3	9
β-strand	62-64	3	9
β-strand	66-69	4	8
α-helix	73-81	9
α-helix	84-87	4
α-helix	110-129	20
β-strand	135-141	7	8
α-helix	145-161	17
β-strand	166-173	8	8
α-helix	184-197	14
β-strand	201-206	6	8
α-helix	207-213	7
α-helix	214-218	5
α-helix	225-239	15
α-helix	241-244	4
β-strand	249	1	8
α-helix	253-259	7
β-strand	270-272	3	8
α-helix	292-296	5
β-strand	312	1	10
β-strand	313	1	11
β-strand	316-322	7	8
α-helix	326-339	14
β-strand	343	1	10
β-strand	352-357	6	8
α-helix	359-361	3
β-strand	374-380	7	8
β-strand	382	1	11
α-helix	384-400	17
α-helix	406-412	7
α-helix	416-438	23

Chain Ac: 16 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	50
α-helix	10-27	18
β-strand	54-56	3	51
β-strand	62-64	3	51
β-strand	66-69	4	50
α-helix	73-81	9
α-helix	83-87	5
α-helix	110-129	20
β-strand	135-141	7	50
α-helix	145-161	17
β-strand	167-173	7	50
α-helix	184-198	15
β-strand	201-206	6	50
α-helix	207-216	10
α-helix	225-244	20
α-helix	253-260	8
β-strand	270-274	5	50
α-helix	279-284	6
α-helix	289-296	8
β-strand	312	1	52
β-strand	316-322	7	50
α-helix	326-339	14
β-strand	343	1	52
β-strand	352-357	6	50
α-helix	359-361	3
β-strand	374-380	7	50
α-helix	384-402	19
α-helix	406-412	7
α-helix	416-438	23

Chain Ad: 16 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	39
α-helix	11-27	17
β-strand	54-56	3	40
β-strand	62-64	3	40
β-strand	66-69	4	39
α-helix	73-81	9
α-helix	83-87	5
α-helix	110-129	20
β-strand	135-141	7	39
α-helix	145-161	17
β-strand	166-173	8	39
α-helix	184-198	15
β-strand	201-206	6	39
α-helix	207-216	10
α-helix	225-244	20
β-strand	249	1	39
α-helix	253-259	7
β-strand	270-274	5	39
α-helix	279-284	6
α-helix	289-296	8
β-strand	312	1	41
β-strand	317-322	6	39
α-helix	326-339	14
β-strand	343	1	41
β-strand	353-357	5	39
α-helix	359-361	3
β-strand	374-380	7	39
α-helix	384-402	19
α-helix	406-412	7
α-helix	416-438	23

Chain Ae: 16 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	101
α-helix	11-27	17
β-strand	54-56	3	102
β-strand	62-64	3	102
β-strand	66-69	4	101
α-helix	73-81	9
α-helix	83-87	5
α-helix	110-129	20
β-strand	135-141	7	101
α-helix	145-161	17
β-strand	167-173	7	101
α-helix	184-198	15
β-strand	201-206	6	101
α-helix	207-216	10
α-helix	225-244	20
β-strand	249	1	101
α-helix	253-259	7
β-strand	270-274	5	101
α-helix	279-284	6
α-helix	289-296	8
β-strand	312	1	103
β-strand	316-322	7	101
α-helix	326-339	14
β-strand	343	1	103
β-strand	352-357	6	101
α-helix	359-361	3
β-strand	374-380	7	101
α-helix	384-402	19
α-helix	406-412	7
α-helix	416-438	23

Chain Af: 17 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	94
α-helix	11-27	17
β-strand	54-56	3	95
β-strand	62-64	3	95
β-strand	66-69	4	94
α-helix	73-81	9
α-helix	83-87	5
α-helix	110-129	20
β-strand	135-141	7	94
α-helix	145-161	17
β-strand	167-173	7	94
α-helix	184-198	15
β-strand	201-206	6	94
α-helix	207-216	10
α-helix	225-239	15
α-helix	241-244	4
β-strand	249	1	94
α-helix	253-259	7
β-strand	270-274	5	94
α-helix	279-284	6
α-helix	289-296	8
β-strand	312	1	96
β-strand	317-322	6	94
α-helix	326-339	14
β-strand	343	1	96
β-strand	353-357	5	94
α-helix	359-361	3
β-strand	374-380	7	94
α-helix	384-402	19
α-helix	406-412	7
α-helix	416-438	23

Chain Ag: 17 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	133
α-helix	11-27	17
β-strand	54-56	3	134
β-strand	62-64	3	134
β-strand	66-69	4	133
α-helix	73-81	9
α-helix	83-87	5
α-helix	110-129	20
β-strand	135-141	7	133
α-helix	145-161	17
β-strand	166-173	8	133
α-helix	184-197	14
β-strand	201-206	6	133
α-helix	207-216	10
α-helix	225-239	15
α-helix	241-244	4
β-strand	249	1	133
α-helix	253-260	8
β-strand	270-274	5	133
α-helix	279-284	6
α-helix	289-296	8
β-strand	312	1	135
β-strand	316-322	7	133
α-helix	326-339	14
β-strand	343	1	135
β-strand	352-357	6	133
α-helix	359-361	3
β-strand	374-380	7	133
α-helix	384-402	19
α-helix	406-412	7
α-helix	416-437	22

Chain Ah: 16 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	125
α-helix	10-27	18
β-strand	54-56	3	126
β-strand	62-64	3	126
β-strand	66-69	4	125
α-helix	73-81	9
α-helix	83-87	5
α-helix	110-129	20
β-strand	135-141	7	125
α-helix	145-161	17
β-strand	167-173	7	125
α-helix	184-198	15
β-strand	201-206	6	125
α-helix	207-216	10
α-helix	225-244	20
β-strand	249	1	125
α-helix	253-259	7
β-strand	270-274	5	125
α-helix	279-284	6
α-helix	289-296	8
β-strand	312	1	127
β-strand	316-322	7	125
α-helix	326-339	14
β-strand	343	1	127
β-strand	352-357	6	125
α-helix	359-361	3
β-strand	374-380	7	125
α-helix	384-402	19
α-helix	406-412	7
α-helix	416-437	22

63 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Tubulin gamma chain	a, b, c, d, e, f, g, h, i, j, k, l, m, n	protein	473	Saccharomyces cerevisiae	P53378 (AlphaFold model)
Spindle pole body component	C, E, G, I, K, M, O	protein	823	Saccharomyces cerevisiae	P38863 (AlphaFold model)
Spindle pole body component	D, F, H, J, L, N, P	protein	846	Saccharomyces cerevisiae	P53540 (AlphaFold model)
Tubulin alpha-1 chain	Ab, Ac, Ad, Ae, Af, Ag, Ah, Ai, Aj, Ak, Al, Am, An, Ao, Ap, Aq, Ar	protein	447	Saccharomyces cerevisiae	P09733 (AlphaFold model)
Tubulin beta chain	Bb, Bc, Bd, Be, Bf, Bg, Bh, Bi, Bj, Bk, Bl, Bm, Bn, Bo, Bp, Bq, Br	protein	457	Saccharomyces cerevisiae	P02557
Spindle pole body component 110	Sa, Sb, Sc, Sd, Se, Sf, Sg, Sh, Si, Sj, Sk, Sl, Sm, Sn	protein	944	Saccharomyces cerevisiae	P32380
Unkown protein	Ua, Ub, Uc, Ud, Ue, Uf, Ug, Uh, Ui, Uj, Uk, Ul, Um, Un	protein	67	Saccharomyces cerevisiae

Sequence of entity 1 (a, b, c, d, e, f, g, h, i, j, k, l, m, n), FASTA

>8QV2_1 Tubulin gamma chain (chains a, b, c, d, e, f, g, h, i, j, k, l, m, n)
MGGEIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTKPFFRENSRN
KFTPRAIMMDSEPSVIADVENTFRGFFDPRNTWVASDGASAGNSWANGYDIGTRNQDDIL
NKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPKKILTTYSVFPARSSEV
VVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIISSVTN
SIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVMLDLLD
PSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQQRIKFPSWSSSAMHVNIGRRS
PYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQSMQNVQ
DEFAESREVVQSLMEDYVAAEQDSYLDDVLVDDENMVGELEEDLDADGDHKLV

Sequence of entity 2 (C, E, G, I, K, M, O), FASTA

>8QV2_2 Spindle pole body component (chains C, E, G, I, K, M, O)
MEIKEVDDRAELLRYTNNIPLLGKLVNHQPLWSTNPKLKSFSLEKISAPDQRRVQEALVV
KDLLNVLIGLEGTYIRYFNDYEPSDPETPIEFKIAKKMDPSFKTFSRRIVRYGKQYMILT
RAYEKWSDTSFGMVLQRFAYEIRRFLEDVYLKTLVERLERDFNKVPNFSIRELEQIINET
EVNKQMELLYNIYEEIFREIEERRTNQSSQEDFNNFMDSMKNESSLHLRLMVAFDTTVYP
VPKGGAILKIFQQKILENLGDRSSVMFLKKLLNNISQDYCTMLYEWLTQGILNDPYQEFM
TYDDLEGKTDNIFDTRDRAWDTQYFIRKDVLLRDCDSEEDKNLLFKMLRTGILLKVVRAS
LQIPTIPSNSSDITIQEINDFADLMEGSNLELYVDKCYSRANEIFLKLFFQGYDLINVLK
HLQQIFLGYQSGHNVLKFLTKNMGELTKHYRNDNNANYDKLLQNFELERQSENPNNLMRQ
LLMIQFDTETLPQVLSHYLQIYPEVPENNSANDDSDPLMHANNFKNMNAILFDELSKERT
GAYHGSNLELYTPKSAIYHLKFDINIPYPLNIIISRTCMIKYQIILRYQLVLQYHSRLLD
ETWMDLNKTPSWKYRGYSHTVKRRIVRATRVLHAKMNHFIKTIMEYFNQNVIDKEVYSLE
KCYRNPTLAVAIQNELEGGLTNIMTNRCLSDLIPLQLQIFDIVYKFCKFIKSMRAKLCQL
DPVLYEKHKSGMMKTLNEGYRTNNGGQEDVGYQEDAALELIQKLIEYISNASSIFRKCLI
NFTQELSTEKFDFYDSSSVDAAGIERVLYSIVPPRSASASSQR

Sequence of entity 3 (D, F, H, J, L, N, P), FASTA

>8QV2_3 Spindle pole body component (chains D, F, H, J, L, N, P)
MELEPTLFGIIEALAPQLLSQSHLQTFVSDVVNLLRSSTKSATQLGPLIDFYKLQSLDSP
ETTIMWHKIEKFLDALFGIQNTDDMVKYLSVFQSLLPSNYRAKIVQKSSGLNMENLANHE
HLLSPVRAPSIYTEASFENMDRFSERRSMVSSPNRYVPSSTYSSVTLRQLSNPYYVNTIP
EEDILKYVSYTLLATTSALFPFDHEQIQIPSKIPNFESGLLHLIFEAGLLYQSLGYKVEK
FRMLNISPMKKALIIEISEELQNYTAFVNNLVSSGTVVSLKSLYREIYENIIRLRIYCRF
TEHLEELSGDTFLIELNIFKSHGDLTIRKIATNLFNSMISLYYEYLMNWLTKGLLRATYG
EFFIAENTDTNGTDDDFIYHIPIEFNQERVPAFIPKELAYKIFMIGKSYIFLEKYCKEVQ
WTNEFSKKYHVLYQSNSYRGISTNFFEIINDQYSEIVNHTNQILNQKFHYRDVVFALKNI
LLMGKSDFMDALIEKANDILATPSDSLPNYKLTRVLQEAVQLSSLRHLMNSPRNSSVING
LDARVLDLGHGSVGWDVFTLDYILYPPLSLVLNVNRPFGRKEYLRIFNFLWRFKKNNYFY
QKEMLKSNDIIRSFKKIRGYNPLIRDIINKLSRISILRTQFQQFNSKMESYYLNCIIEEN
FKEMTRKLQRTENKSQNQFDLIRLNNGTIELNGILTPKAEVLTKSSSSKPQKHAIEKTLN
IDELESVHNTFLTNILSHKLFATNTSEISVGDYSGQPYPTSLVLLLNSVYEFVKVYCNLN
DIGYEIFIKMNLNDHEASNGLLGKFNTNLKEIVSQYKNFKDRLYIFRADLKNDGDEELFL
LSKSLR

Sequence of entity 4 (Ab, Ac, Ad, Ae, Af, Ag, Ah, Ai, Aj, Ak, Al, Am, An, Ao, Ap, Aq, Ar), FASTA

>8QV2_4 Tubulin alpha-1 chain (chains Ab, Ac, Ad, Ae, Af, Ag, Ah, Ai, Aj, Ak, Al, Am, An, Ao, Ap, Aq, Ar)
MREVISINVGQAGCQIGNACWELYSLEHGIKPDGHLEDGLSKPKGGEEGFSTFFHETGYG
KFVPRAIYVDLEPNVIDEVRNGPYKDLFHPEQLISGKEDAANNYARGHYTVGREILGDVL
DRIRKLADQCDGLQGFLFTHSLGGGTGSGLGSLLLEELSAEYGKKSKLEFAVYPAPQVST
SVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDMCKRNLDIPRPSFANLNNLIAQVVSSVT
ASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYSPVLSKSKAFHESNSVSEITNACFEPG
NQMVKCDPRDGKYMATCLLYRGDVVTRDVQRAVEQVKNKKTVQLVDWCPTGFKIGICYEP
PTATPNSQLATVDRAVCMLSNTTSIAEAWKRIDRKFDLMYAKRAFVHWYVGEGMEEGEFT
EAREDLAALERDYIEVGADSYAEEEEF

Sequence of entity 5 (Bb, Bc, Bd, Be, Bf, Bg, Bh, Bi, Bj, Bk, Bl, Bm, Bn, Bo, Bp, Bq, Br), FASTA

>8QV2_5 Tubulin beta chain (chains Bb, Bc, Bd, Be, Bf, Bg, Bh, Bi, Bj, Bk, Bl, Bm, Bn, Bo, Bp, Bq, Br)
MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQKERLNVYFNEASSGKWV
PRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMDVI
RREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVLPSPKTSDTVV
EPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSVMSGVTTSL
RYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFRSLTVPELTQQMFDAKNMM
AAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAVCSVAPQG
LDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEFSEAESNMNDLVS
EYQQYQEATVEDDEEVDENGDFGAPQNQDEPITENFE

Sequence of entity 6 (Sa, Sb, Sc, Sd, Se, Sf, Sg, Sh, Si, Sj, Sk, Sl, Sm, Sn), FASTA

>8QV2_6 Spindle pole body component 110 (chains Sa, Sb, Sc, Sd, Se, Sf, Sg, Sh, Si, Sj, Sk, Sl, Sm, Sn)
MDEASHLPNGSLKNMEFTPVGFIKSKRNTTQTQVVSPTKVPNANNGDENEGPVKKRQRRS
IDDTIDSTRLFSEASQFDDSFPEIKANIPPSPRSGNVDKSRKRNLIDDLKKDVPMSQPLK
EQEVREHQMKKERFDRALESKLLGKRHITYANSDISNKELYINEIKSLKHEIKELRKEKN
DTLNNYDTLEEETDDLKNRLQALEKELDAKNKIVNSRKVDDHSGCIEEREQMERKLAELE
RKLKTVKDQVLELENNSDVQSLKLRSKEDELKNLMNELNELKSNAEEKDTQLEFKKNELR
KRTNELNELKIKSDEMDLQLKQKQNESKRLKDELNELETKFSENGSQSSAKENELKMLKN
KIAELEEEISTKNSQLIAKEGKLASLMAQLTQLESKLNQRDSQLGSREEELKKTNDKLQK
DIRIAREETVSKDERIIDLQKKVKQLENDLFVIKKTHSESKTITDNELESKDKLIKILEN
DLKVAQEKYSKMEKELKEREFNYKISESKLEDEKTTLNEKISNLAAENSQLKNKIEDNST
ATHHMKENYEKQLESLRKDIEEYKESAKDSEDKIEELKIRIAENSAKVSEKRSKDIKQKD
EQISDLTQNLKLQEDEISSLKSIIDRYKKDFNQLKSEQSNIQHDLNLQILNLENKLIESE
DELKSLRDSQKIEIENWKRKYNNLSLENDRLLTEKESASDKEREISILNRKLDEMDKEKW
NLQESKEKYKRELQKVITANDRLRREKEELNENSNNIRIMEDKMTRIKKNYLSEITSLQE
ENRRLEERLILNERRKDNDSTMQLNDIISYYKLKYHSEVRHNNDLKVINDYLNKVLALGT
RRLRLDTRKGEHSLNISLPDDDELDRDYYNSHVYTRYHDYEYPLRFNLNRRGPYFERRLS
FKTVALLVLACVRMKRIAFYRRSDDNRLRILRDRIESSSGRISW

Sequence of entity 7 (Ua, Ub, Uc, Ud, Ue, Uf, Ug, Uh, Ui, Uj, Uk, Ul, Um, Un), FASTA

>8QV2_7 Unkown protein (chains Ua, Ub, Uc, Ud, Ue, Uf, Ug, Uh, Ui, Uj, Uk, Ul, Um, Un)
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXX

Ligands and cofactors

ID	Name	Formula	Copies
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	13
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	1

Primary citation

Structure of the native gamma-tubulin ring complex capping spindle microtubules. Dendooven, T., Yatskevich, S., Burt, A. et al. Nat Struct Mol Biol (2024) 31:1134-1144. DOI 10.1038/s41594-024-01281-y · PubMed

Other PDB entries of the same protein (UniProt P53378 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7M2W 3.0 Å, Engineered disulfide cross-linked closed conformation of the Yeast gamma-TuRC(SS)
7M2X 3.6 Å, Open conformation of the Yeast wild-type gamma-TuRC
7M2Z 3.7 Å, Monomeric single-particle reconstruction of the Yeast gamma-TuSC
7M2Y 4.03 Å, Closed conformation of the Yeast wild-type gamma-TuRC
5FLZ 6.9 Å, Cryo-EM structure of gamma-TuSC oligomers in a closed conformation
5FM1 8.0 Å, Structure of gamma-tubulin small complex based on a cryo-EM map, chemical cross-links,…
8QV3 8.2 Å, Structure of the y-Tubulin Small Complex (yTuSC) as part of the native y-Tubulin Ring…
9A15 Integrative structure of the yeast gammaTuSC-Spc110 monomer complex
9A16 Integrative structure of the yeast gammaTuSC-Spc110 dimer complex
9A17 Integrative structure of the yeast gammaTuSC-Spc110 tetramer complex

Browse structure collections

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