8QYZ: HiNES2

Crystal structure of hiNES2 in complex with Xpo1 and RanGTP. Determined by X-ray diffraction at 3.0 Å resolution. Released 6 Nov 2024.

Method

X-ray diffraction

Resolution

3.0 Å

Organisms

Saccharomyces cerevisiae S288C, synthetic construct

Chains

12

Atoms

39,266

Mol. weight

573.43 kDa

Ligands

GTP, MG

Released

6 Nov 2024

Explore 8QYZ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8QYZ contains 301 α-helices and 52 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 9 β-strands

Molecules and chains

>8QYZ_1 GTP-binding nuclear protein GSP1/CNR1 (chains A, B, E, G)
MSAPAANGEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATIGVEVHPLSFYTNFGE
IKFDVWDTAGLEKFGGLRDGYYINAQCAIIMFDVTSRITYKNVPNWHRDLVRVCENIPIV
LCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLAGNPQLEFVA
SP

>8QYZ_2 Exportin-1 (chains C, D, F, H)
GMEGILDFSNDLDIALLDQVVSTFYQGSGVQQKQAQEILTKFQDNPDAWQKADQILQFST
NPQSKFIALSILDKLITRKWKLLPNDHRIGIRNFVVGMIISMCQDDEVFKTQKNLINKSD
LTLVQILKQEWPQNWPEFIPELIGSSSSSVNVCENNMIVLKLLSEEVFDFSAEQMTQAKA
LHLKNSMSKEFEQIFKLCFQVLEQGSSSSLIVATLESLLRYLHWIPYRYIYETNILELLS
TKFMTSPDTRAITLKCLTEVSNLKIPQDNDLIKRQTVLFFQNTLQQIATSVMPVTADLKA
TYANANGNDQSFLQDLAMFLTTYLARNRALLESDESLRELLLNAHQYLIQLSKIEERELF
KTTLDYWHNLVADLFYEPLKKHIYEEICSQLRLVIIENMVRPEEVLVVENDEGEIVREFV
KESDTIQLYKSEREVLVYLTHLNVIDTEEIMISKLARQIDGSEWSWHNINTLSWAIGSIS
GTMSEDTEKRFVVTVIKDLLDLTVKKRGKDNKAVVASDIMYVVGQYPRFLKAHWNFLRTV
ILKLFEFMHETHEGVQDMACDTFIKIVQKCKYHFVIQQPRESEPFIQTIIRDIQKTTADL
QPQQVHTFYKACGIIISEERSVAERNRLLSDLMQLPNMAWDTIVEQSTANPTLLLDSETV
KIIANIIKTNVAVCTSMGADFYPQLGHIYYNMLQLYRAVSSMISAQVAAEGLIATKTPKV
RGLRTIKKEILKLVETYISKARNLDDVVKVLVEPLLNAVLEDYMNNVPDARDAEVLNCMT
TVVEKVGHMIPQGVILILQSVFECTLDMINKDFTEYPEHRVEFYKLLKVINEKSFAAFLE
LPPAAFKLFVDAICWAFKHNNRDVEVNGLQIALDLVKNIERMGNVPFANEFHKNYFFIFV
SETFFVLTDSDHKSGFSKQALLLMKLISLVYDNKISVPLYQEAEVPQGTSNQVYLSQYLA
NMLSNAFPHLTSEQIASFLSALTKQYKDLVVFKGTLRDFLVQIKEVGGDPTDYLFAEDKE
NALMEQNRLEREKAAKIGGLLKPSELDD

>8QYZ_3 hiNES2 (chains I, J, K, L)
GEVDDLCELMAQLSIN

Ligands and cofactors

Water and common crystallization additives (ACT) are not listed.

Primary citation

Exploring sequence space and engineering of Xpo1-dependent NESes. Rymarenko, O., Huyton, T., Gorlich, D. To be published.

Related structures

Other PDB entries of the same protein (UniProt P32835 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3M1I 2.0 Å, Crystal structure of yeast CRM1 (Xpo1p) in complex with yeast RanBP1 (Yrb1p) and yeast…
• 3WYG 2.15 Å, Crystal structure of Xpo1p-PKI-Gsp1p-GTP complex
• 5XOJ 2.2 Å, Crystal structure of Xpo1p-PKI-Nup42p-Gsp1p-GTP complex
• 3WYF 2.22 Å, Crystal structure of Xpo1p-Yrb2p-Gsp1p-GTP complex
• 2X19 2.8 Å, Crystal structure of Importin13 - RanGTP complex
• 9B3I 2.88 Å, Cryo-EM structure of yeast (Nap1)2-H2A-H2B-Kap114-RanGTP
• 9D45 3.1 Å, Cryo-EM structure of yeast Exportin Msn5 bound to cargo Pho4 and RanGTP
• 9DZ6 3.1 Å, Cryo-EM structure of yeast Exportin Msn5 bound to RanGTP and Pho4 (not modeled) (State…
• 3ICQ 3.2 Å, Karyopherin nuclear state
• 9DXM 3.2 Å, Cryo-EM structure of yeast Exportin Msn5 bound to RanGTP and Pho4 (not modeled) (State…
• 9OGB 3.25 Å, Cryo-EM structure of human exportin-1 conjugated with selinexor and bound to yeast…
• 8F1E 3.28 Å, Cryo-EM structure of Kap114 bound to Gsp1 (RanGTP) and H2A-H2B

Browse structure collections

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