8SYF: Myosin, heavy chain 11, smooth muscle

Homology model of Acto-HMM complex in ADP-state. Chicken smooth muscle HMM and chicken pectoralis actin. Determined by electron microscopy at 19.0 Å resolution. Released 30 Aug 2023.

Method: Electron microscopy
Resolution: 19.0 Å
Organism: Gallus gallus
Chains: 8
Atoms: 23,456
Mol. weight: 343.71 kDa
Released: 30 Aug 2023

Explore 8SYF in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8SYF contains 141 α-helices and 103 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 32 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	8-11	4
β-strand	48-53	6	1
β-strand	56-61	6	1
β-strand	66-70	5	1
α-helix	81-83	3
β-strand	88	1	2
α-helix	89-91	3
α-helix	97-109	13
β-strand	114-116	3	2
β-strand	121-123	3	2
β-strand	124	1	3
α-helix	135-139	5
α-helix	153-165	13
β-strand	173	1	4
α-helix	183-197	15
α-helix	220-234	15
β-strand	236	1	5
β-strand	237	1	6
β-strand	246	1	5
β-strand	249-257	9	7
β-strand	261-270	10	7
α-helix	274-278	5
β-strand	287	1	6
α-helix	288-296	9
α-helix	299-304	6
α-helix	328-342	15
α-helix	348-362	15
β-strand	366	1	8
β-strand	380	1	8
α-helix	382-391	10
α-helix	395-403	9
β-strand	407-408	2	9
β-strand	413-414	2	9
α-helix	420-450	31
β-strand	459-465	7	7
β-strand	475	1	10
α-helix	477-507	31
α-helix	517-520	4
α-helix	522-529	8
α-helix	537-545	9
α-helix	553-561	9
β-strand	569-571	3	10
β-strand	580-583	4	10
β-strand	590-593	4	10
α-helix	597-600	4
α-helix	607-614	8
α-helix	619-624	6
α-helix	660-675	16
β-strand	680	1	4
β-strand	685	1	3
α-helix	698-706	9
α-helix	710-719	10
β-strand	723-726	4	11
α-helix	727-737	11
α-helix	749-758	10
β-strand	767-769	3	11
β-strand	773-776	4	11
α-helix	780-811	32
α-helix	815-835	21
α-helix	848-850	3

Chain B: 36 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	8-11	4
β-strand	34	1	12
β-strand	52	1	13
β-strand	57-61	5	13
β-strand	66-70	5	13
β-strand	78	1	12
β-strand	88	1	14
α-helix	89-91	3
α-helix	97-109	13
β-strand	114-117	4	14
β-strand	120-124	5	14
α-helix	131-133	3
α-helix	135-139	5
α-helix	155-164	10
β-strand	171-176	6	14
α-helix	183-197	15
α-helix	221-230	10
α-helix	231-235	5
β-strand	237	1	15
β-strand	249-256	8	14
β-strand	262-270	9	14
α-helix	274-278	5
β-strand	281	1	16
β-strand	284	1	16
β-strand	287	1	15
α-helix	289-291	3
α-helix	292-296	5
α-helix	299-304	6
α-helix	323	1
α-helix	328-342	15
α-helix	346-364	19
α-helix	382-390	9
α-helix	395-403	9
β-strand	407-408	2	17
β-strand	413-414	2	17
α-helix	420-450	31
β-strand	459-465	7	14
α-helix	477-507	31
α-helix	514-516	3
α-helix	518-521	4
α-helix	523-529	7
α-helix	537-545	9
α-helix	552-562	11
β-strand	569-570	2	18
β-strand	580-583	4	18
β-strand	586	1	19
β-strand	588	1	19
β-strand	590-593	4	18
α-helix	598-600	3
α-helix	607-614	8
α-helix	619-624	6
α-helix	659-675	17
β-strand	678-685	8	14
α-helix	698-707	10
α-helix	710-719	10
β-strand	723-726	4	20
α-helix	727-735	9
α-helix	749-760	12
β-strand	769	1	20
β-strand	773-776	4	20
α-helix	780-836	57
α-helix	844-846	3
α-helix	848-850	3

Chain C: 21 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	9-12	4	21
β-strand	17-20	4	21
β-strand	29-31	3	21
β-strand	35-38	4	22
β-strand	53	1	22
α-helix	55-59	5
β-strand	65-68	4	22
β-strand	71-72	2	23
β-strand	75-76	2	23
α-helix	79-86	8
α-helix	87-93	7
β-strand	104-107	4	21
α-helix	113-121	9
α-helix	122-126	5
β-strand	131-132	2	24
β-strand	133-136	4	21
α-helix	137-144	8
β-strand	151-154	4	25
β-strand	160-163	4	25
β-strand	176-178	3	25
α-helix	182-196	15
α-helix	203-215	13
α-helix	223-229	7
β-strand	238-242	5	26
β-strand	246-250	5	26
α-helix	252-254	3
α-helix	256-262	7
α-helix	264-266	3
α-helix	274-284	11
α-helix	287-295	9
β-strand	297-298	2	25
α-helix	303-305	3
α-helix	309-319	11
β-strand	329-330	2	25
α-helix	338-348	11
α-helix	350-354	5
α-helix	356	1
β-strand	357-358	2	24
α-helix	359-365	7
α-helix	369-373	5

Chain D: 18 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	8-11	4	27
β-strand	16-20	5	27
β-strand	29-32	4	27
β-strand	35-38	4	28
β-strand	53-54	2	28
α-helix	56-60	5
β-strand	65-68	4	28
β-strand	71-72	2	29
β-strand	75-76	2	29
α-helix	79-86	8
α-helix	87-93	7
β-strand	103-107	5	27
α-helix	113-121	9
α-helix	122-126	5
β-strand	131-136	6	27
α-helix	137-144	8
β-strand	151-155	5	30
β-strand	160-164	5	30
β-strand	176-178	3	30
α-helix	182-196	15
α-helix	203-215	13
α-helix	223-229	7
β-strand	238-242	5	31
β-strand	246-250	5	31
α-helix	252-262	11
α-helix	264-266	3
α-helix	274-283	10
α-helix	287-295	9
β-strand	296	1	32
β-strand	297-300	4	30
α-helix	309-319	11
β-strand	328	1	32
α-helix	338-348	11
α-helix	350-353	4
β-strand	357-358	2	27
α-helix	359-365	7
α-helix	368-373	6

Chain E: 9 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	8-17	10
β-strand	25-26	2	33
α-helix	28-37	10
α-helix	44-49	6
α-helix	56-60	5
β-strand	64-65	2	33
α-helix	66-76	11
α-helix	85-95	11
α-helix	105-113	9
α-helix	121-128	8
α-helix	140-149	10

Chain F: 8 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	27-40	14
β-strand	47	1	34
α-helix	50-60	11
α-helix	66-73	8
β-strand	81	1	34
α-helix	82-93	12
α-helix	99-109	11
β-strand	117-118	2	35
α-helix	119-128	10
α-helix	135-143	9
β-strand	152-153	2	35
α-helix	158-161	4

Chain G: 8 helices, 0 β-strands

Element	Residues	Length
α-helix	27-40	14
α-helix	50-60	11
α-helix	66-73	8
α-helix	82-91	10
α-helix	99-107	9
α-helix	120-127	8
α-helix	136-142	7
α-helix	155-162	8

Chain H: 9 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	5-17	13
β-strand	25-26	2	36
α-helix	28-37	10
α-helix	44-49	6
β-strand	64-65	2	36
α-helix	69-77	9
α-helix	85-94	10
β-strand	103-104	2	37
α-helix	105-113	9
α-helix	121-128	8
α-helix	134-136	3
β-strand	137-138	2	37
α-helix	140-149	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Myosin, heavy chain 11, smooth muscle	A, B	protein	843	Gallus gallus	P10587 (AlphaFold model)
Actin, alpha skeletal muscle	C, D	protein	375	Gallus gallus	P68139 (AlphaFold model)
Myosin light polypeptide 6	E, H	protein	148	Gallus gallus	P02607 (AlphaFold model)
Myosin regulatory light chain 2, smooth muscle major isoform	F, G	protein	143	Gallus gallus	P02612 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>8SYF_1 Myosin, heavy chain 11, smooth muscle (chains A, B)
SQKPLSDDEKFLFVDKNFVNNPLAQADWSAKKLVWVPSEKHGFEAASIKEEKGDEVTVEL
QENGKKVTLSKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFC
VVINPYKQLPIYSEKIIDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGA
GKTENTKKVIQYLAVVASSHKGKKDTSITGELEKQLLQANPILEAFGNAKTVKNDNSSRF
GKFIRINFDVTGYIVGANIETYLLEKSRAIRQAKDERTFHIFYYLIAGASEQMRNDLLLE
GFNNYTFLSNGHVPIPAQQDDEMFQETLEAMTIMGFTEEEQTSILRVVSSVLQLGNIVFK
KERNTDQASMPDNTAAQKVCHLMGINVTDFTRSILTPRIKVGRDVVQKAQTKEQADFAIE
ALAKAKFERLFRWILTRVNKALDKTKRQGASFLGILDIAGFEIFEINSFEQLCINYTNEK
LQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPTNPPGVLALLDEECWFP
KATDTSFVEKLIQEQGNHAKFQKSKQLKDKTEFCILHYAGKVTYNASAWLTKNMDPLNDN
VTSLLNQSSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQLYKEQLT
KLMTTLRNTNPNFVRCIIPNHEKRAGKLDAHLVLEQLRCNGVLEGIRICRQGFPNRIVFQ
EFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEER
DLKITDVIIAFQAQCRGYLARKAFAKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVK
PLL

Sequence of entity 2 (C, D), FASTA

>8SYF_2 Actin, alpha skeletal muscle (chains C, D)
DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS
KRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMT
QIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDL
AGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSY
ELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMS
GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQ
EYDEAGPSIVHRKCF

Sequence of entity 3 (E, H), FASTA

>8SYF_3 Myosin light polypeptide 6 (chains E, H)
FSEEQTAEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLK
TLNFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEE
EVEQLVAGHEDSNGCINYEELVRMVLSG

Sequence of entity 4 (F, G), FASTA

>8SYF_4 Myosin regulatory light chain 2, smooth muscle major isoform (chains F, G)
FDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEGMMSEAPGPINFTM
FLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYRE
APIDKKGNFNYVEFTRILKHGAK

Primary citation

Double-headed binding of myosin II to F-actin shows the effect of strain on head structure. Hojjatian, A., Taylor, D.W., Daneshparvar, N. et al. J Struct Biol (2023) 215:107995-107995. DOI 10.1016/j.jsb.2023.107995 · PubMed

Other PDB entries of the same protein (UniProt P10587 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5M05 2.67 Å, Chicken smooth muscle myosin motor domain co-crystallized with the specific CK-571…
5T45 2.8 Å, Chicken smooth muscle myosin motor domain co-crystallized with the specific CK-571…
1BR2 2.9 Å, Smooth muscle myosin motor domain complexed with MGADP.ALF4
7MF3 3.4 Å, Structure of the autoinhibited state of smooth muscle myosin-2
1BR1 3.5 Å, Smooth muscle myosin motor domain-essential light chain complex with MGADP.ALF4 bound at…
1BR4 3.62 Å, Smooth muscle myosin motor domain-essential light chain complex with MGADP.BEF3 bound at…
6BIH 6.0 Å, The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State
1I84 20.0 Å, cryo-EM structure of the heavy meromyosin subfragment of chicken gizzard smooth muscle…
3DTP 20.0 Å, Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick…
3J04 20.0 Å, EM structure of the heavy meromyosin subfragment of Chick smooth muscle Myosin with…

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