8USB: 26S proteasome non-ATPase regulatory subunit 12
Non-substrate-engaged human 26S proteasome with Nub1/FAT10 bound to Rpn1. Determined by electron microscopy at 2.73 Å resolution. Released 6 Nov 2024.
- Method
- Electron microscopy
- Resolution
- 2.73 Å
- Organism
- Homo sapiens
- Chains
- 27
- Atoms
- 70,143
- Mol. weight
- 1211.82 kDa
- Ligands
- ZN, ATP, MG, ADP
- Released
- 6 Nov 2024
Explore 8USB in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8USB contains 507 α-helices and 274 β-strands across 27 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain a: 25 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-13 | 8 | |
| α-helix | 19-31 | 13 | |
| α-helix | 34-45 | 12 | |
| α-helix | 55-59 | 5 | |
| α-helix | 60-64 | 5 | |
| α-helix | 65-67 | 3 | |
| α-helix | 68-70 | 3 | |
| α-helix | 73-86 | 14 | |
| α-helix | 90-103 | 14 | |
| α-helix | 108-124 | 17 | |
| α-helix | 128-144 | 17 | |
| α-helix | 152-166 | 15 | |
| α-helix | 169-182 | 14 | |
| α-helix | 190-206 | 17 | |
| α-helix | 213-217 | 5 | |
| α-helix | 220-225 | 6 | |
| α-helix | 231-241 | 11 | |
| α-helix | 245-250 | 6 | |
| α-helix | 252-255 | 4 | |
| α-helix | 259-263 | 5 | |
| α-helix | 265-283 | 19 | |
| α-helix | 286-288 | 3 | |
| β-strand | 290-292 | 3 | 2 |
| α-helix | 293-299 | 7 | |
| α-helix | 304-317 | 14 | |
| β-strand | 320-325 | 6 | 2 |
| β-strand | 330-336 | 7 | 2 |
| α-helix | 343-370 | 28 | |
Chain A: 26 helices, 19 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 42-63 | 22 | |
| β-strand | 72 | 1 | 17 |
| α-helix | 73-74 | 2 | |
| α-helix | 75-77 | 3 | |
| α-helix | 80-89 | 10 | |
| β-strand | 93-102 | 10 | 3 |
| β-strand | 110-115 | 6 | 3 |
| β-strand | 119-123 | 5 | 3 |
| β-strand | 125 | 1 | 3 |
| α-helix | 131-133 | 3 | |
| β-strand | 139-142 | 4 | 3 |
| β-strand | 149-152 | 4 | 3 |
| α-helix | 154-156 | 3 | |
| α-helix | 159-162 | 4 | |
| β-strand | 165 | 1 | 20 |
| α-helix | 174-176 | 3 | |
| α-helix | 181-191 | 11 | |
| α-helix | 193-196 | 4 | |
| α-helix | 199-202 | 4 | |
| β-strand | 211-215 | 5 | 20 |
| α-helix | 222-232 | 11 | |
| β-strand | 236-241 | 6 | 20 |
| α-helix | 242-245 | 4 | |
| α-helix | 252-264 | 13 | |
| β-strand | 270-275 | 6 | 20 |
| α-helix | 277-279 | 3 | |
| β-strand | 282 | 1 | 21 |
| α-helix | 283-284 | 2 | |
| β-strand | 287 | 1 | 22 |
| β-strand | 289 | 1 | 22 |
| α-helix | 293-306 | 14 | |
| β-strand | 315-321 | 7 | 20 |
| α-helix | 324-326 | 3 | |
| β-strand | 327 | 1 | 21 |
| β-strand | 339-342 | 4 | 20 |
| α-helix | 344-346 | 3 | |
| α-helix | 348-358 | 11 | |
| β-strand | 364 | 1 | 23 |
| α-helix | 370-375 | 6 | |
| α-helix | 382-398 | 17 | |
| β-strand | 404 | 1 | 23 |
| α-helix | 406-413 | 8 | |
| α-helix | 414-418 | 5 | |
| α-helix | 419-423 | 5 | |
| α-helix | 426-429 | 4 | |
Chain b: 9 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 4 | 1 | 6 |
| β-strand | 5-10 | 6 | 7 |
| α-helix | 14-17 | 4 | |
| α-helix | 25-43 | 19 | |
| β-strand | 48-53 | 6 | 7 |
| β-strand | 59-65 | 7 | 7 |
| α-helix | 69-76 | 8 | |
| α-helix | 86-98 | 13 | |
| β-strand | 106 | 1 | 6 |
| α-helix | 107 | 1 | |
| β-strand | 109-113 | 5 | 7 |
| α-helix | 121-133 | 13 | |
| β-strand | 138-142 | 5 | 7 |
| α-helix | 146-149 | 4 | |
| α-helix | 152-161 | 10 | |
| α-helix | 180-183 | 4 | |
Chain B: 26 helices, 14 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 37-38 | 2 | |
| α-helix | 45-47 | 3 | |
| α-helix | 59-85 | 27 | |
| α-helix | 92-103 | 12 | |
| β-strand | 107-114 | 8 | 3 |
| β-strand | 120-124 | 5 | 3 |
| β-strand | 130-135 | 6 | 3 |
| β-strand | 139 | 1 | 17 |
| α-helix | 141-143 | 3 | |
| β-strand | 149-153 | 5 | 3 |
| β-strand | 159-162 | 4 | 3 |
| α-helix | 165-168 | 4 | |
| α-helix | 169-174 | 6 | |
| β-strand | 175 | 1 | 18 |
| α-helix | 184-186 | 3 | |
| α-helix | 191-197 | 7 | |
| α-helix | 198-202 | 5 | |
| α-helix | 203-206 | 4 | |
| α-helix | 208-214 | 7 | |
| α-helix | 217-219 | 3 | |
| β-strand | 221-225 | 5 | 18 |
| α-helix | 232-241 | 10 | |
| β-strand | 246-251 | 6 | 18 |
| α-helix | 252-255 | 4 | |
| α-helix | 262-276 | 15 | |
| β-strand | 280-285 | 6 | 18 |
| α-helix | 289-292 | 4 | |
| α-helix | 302-316 | 15 | |
| α-helix | 321-322 | 2 | |
| β-strand | 325-331 | 7 | 18 |
| β-strand | 349-352 | 4 | 18 |
| α-helix | 354-356 | 3 | |
| α-helix | 358-368 | 11 | |
| β-strand | 374 | 1 | 19 |
| α-helix | 380-383 | 4 | |
| α-helix | 392-408 | 17 | |
| β-strand | 414 | 1 | 19 |
| α-helix | 415 | 1 | |
| α-helix | 416-422 | 7 | |
| α-helix | 423-427 | 5 | |
Chain c: 18 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 24-25 | 2 | |
| β-strand | 26 | 1 | 67 |
| β-strand | 30-34 | 5 | 68 |
| α-helix | 35-47 | 13 | |
| β-strand | 54-63 | 10 | 68 |
| β-strand | 66-74 | 9 | 68 |
| α-helix | 75-77 | 3 | |
| α-helix | 84-87 | 4 | |
| α-helix | 89-99 | 11 | |
| α-helix | 100-102 | 3 | |
| β-strand | 107-114 | 8 | 68 |
| α-helix | 124-136 | 13 | |
| β-strand | 141-151 | 11 | 68 |
| β-strand | 154-162 | 9 | 68 |
| α-helix | 163-164 | 2 | |
| α-helix | 165-169 | 5 | |
| β-strand | 176 | 1 | 67 |
| α-helix | 181-183 | 3 | |
| α-helix | 186-188 | 3 | |
| α-helix | 189-193 | 5 | |
| β-strand | 196 | 1 | 68 |
| β-strand | 200-208 | 9 | 68 |
| α-helix | 211-218 | 8 | |
| α-helix | 225-229 | 5 | |
| α-helix | 230-233 | 4 | |
| α-helix | 234-263 | 30 | |
| α-helix | 269-272 | 4 | |
| α-helix | 280-308 | 29 | |
Chain C: 25 helices, 13 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 21-66 | 46 | |
| α-helix | 69-70 | 2 | |
| β-strand | 71-80 | 10 | 3 |
| β-strand | 84-89 | 6 | 3 |
| β-strand | 94-97 | 4 | 3 |
| β-strand | 99 | 1 | 3 |
| α-helix | 105-107 | 3 | |
| β-strand | 113-116 | 4 | 3 |
| β-strand | 123-126 | 4 | 3 |
| α-helix | 127-129 | 3 | |
| α-helix | 133-141 | 9 | |
| α-helix | 143-145 | 3 | |
| α-helix | 148-150 | 3 | |
| α-helix | 155-161 | 7 | |
| α-helix | 162-166 | 5 | |
| α-helix | 167-170 | 4 | |
| α-helix | 172-178 | 7 | |
| α-helix | 181-183 | 3 | |
| β-strand | 185-189 | 5 | 4 |
| α-helix | 196-206 | 11 | |
| β-strand | 210-213 | 4 | 4 |
| α-helix | 221-224 | 4 | |
| α-helix | 231-234 | 4 | |
| α-helix | 235-240 | 6 | |
| β-strand | 244-249 | 6 | 4 |
| α-helix | 268-282 | 15 | |
| β-strand | 289-295 | 7 | 4 |
| α-helix | 303-306 | 4 | |
| β-strand | 313-316 | 4 | 4 |
| α-helix | 318-320 | 3 | |
| α-helix | 322-333 | 12 | |
| β-strand | 338 | 1 | 5 |
| α-helix | 339 | 1 | |
| α-helix | 344-349 | 6 | |
| α-helix | 356-372 | 17 | |
| β-strand | 378 | 1 | 5 |
| α-helix | 380-387 | 8 | |
| α-helix | 388-393 | 6 | |
Chain d: 15 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 87-103 | 17 | |
| α-helix | 109-125 | 17 | |
| α-helix | 138-155 | 18 | |
| α-helix | 161-176 | 16 | |
| α-helix | 178-180 | 3 | |
| α-helix | 188-201 | 14 | |
| α-helix | 205-212 | 8 | |
| α-helix | 217-222 | 6 | |
| α-helix | 224-238 | 15 | |
| α-helix | 241-246 | 6 | |
| α-helix | 254-256 | 3 | |
| α-helix | 257-278 | 22 | |
| β-strand | 279 | 1 | 8 |
| β-strand | 281-283 | 3 | 9 |
| α-helix | 287-290 | 4 | |
| α-helix | 297-306 | 10 | |
| β-strand | 310 | 1 | 9 |
| β-strand | 315-317 | 3 | 9 |
| α-helix | 332-347 | 16 | |
Chain D: 21 helices, 15 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 40-78 | 39 | |
| α-helix | 79-83 | 5 | |
| α-helix | 84 | 1 | |
| α-helix | 86 | 1 | |
| β-strand | 87-94 | 8 | 3 |
| β-strand | 100-105 | 6 | 3 |
| β-strand | 110-115 | 6 | 3 |
| α-helix | 121-123 | 3 | |
| β-strand | 128-133 | 6 | 3 |
| β-strand | 139-142 | 4 | 3 |
| α-helix | 171-177 | 7 | |
| α-helix | 178-182 | 5 | |
| α-helix | 183-186 | 4 | |
| α-helix | 188-194 | 7 | |
| β-strand | 201-205 | 5 | 24 |
| α-helix | 212-221 | 10 | |
| β-strand | 226-231 | 6 | 24 |
| α-helix | 232-234 | 3 | |
| α-helix | 242-256 | 15 | |
| β-strand | 260-265 | 6 | 24 |
| α-helix | 267-269 | 3 | |
| β-strand | 272 | 1 | 25 |
| α-helix | 280-297 | 18 | |
| β-strand | 305-311 | 7 | 24 |
| α-helix | 314-316 | 3 | |
| β-strand | 317 | 1 | 25 |
| α-helix | 319-322 | 4 | |
| β-strand | 329-332 | 4 | 24 |
| α-helix | 338-349 | 12 | |
| β-strand | 354 | 1 | 26 |
| α-helix | 361-364 | 4 | |
| α-helix | 372-388 | 17 | |
| β-strand | 391 | 1 | 1 |
| β-strand | 394 | 1 | 26 |
| α-helix | 396-407 | 12 | |
| α-helix | 415-417 | 3 | |
19 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| 26S proteasome non-ATPase regulatory subunit 12 | W | protein | 456 | Homo sapiens | O00232 (AlphaFold model) |
| 26S protease regulatory subunit 8 | C | protein | 406 | Homo sapiens | P62195 (AlphaFold model) |
| 26S proteasome non-ATPase regulatory subunit 4 | b | protein | 377 | Homo sapiens | P55036 (AlphaFold model) |
| 26S proteasome non-ATPase regulatory subunit 8 | d | protein | 350 | Homo sapiens | P48556 (AlphaFold model) |
| 26S proteasome non-ATPase regulatory subunit 2 | f | protein | 908 | Homo sapiens | Q13200 |
| NEDD8 ultimate buster 1 | g | protein | 605 | Homo sapiens | H3BM14 |
| 26S proteasome regulatory subunit 4 | B | protein | 440 | Homo sapiens | P62191 |
| 26S proteasome regulatory subunit 7 | A | protein | 433 | Homo sapiens | P35998 |
| 26S proteasome regulatory subunit 6B | D | protein | 418 | Homo sapiens | P43686 |
| 26S protease regulatory subunit 10B | E | protein | 389 | Homo sapiens | P62333 |
| 26S proteasome regulatory subunit 6A | F | protein | 439 | Homo sapiens | P17980 |
| Proteasome subunit alpha type-6 | G | protein | 246 | Homo sapiens | P60900 |
15 more molecules are not listed.
Sequence of entity 1 (W), FASTA
>8USB_1 26S proteasome non-ATPase regulatory subunit 12 (chains W)
MADGGSERADGRIVKMEVDYSATVDQRLPECAKLAKEGRLQEVIETLLSLEKQTRTASDM
VSTSRILVAVVKMCYEAKEWDLLNENIMLLSKRRSQLKQAVAKMVQQCCTYVEEITDLPI
KLRLIDTLRMVTEGKIYVEIERARLTKTLATIKEQNGDVKEAASILQELQVETYGSMEKK
ERVEFILEQMRLCLAVKDYIRTQIISKKINTKFFQEENTEKLKLKYYNLMIQLDQHEGSY
LSICKHYRAIYDTPCIQAESEKWQQALKSVVLYVILAPFDNEQSDLVHRISGDKKLEEIP
KYKDLLKLFTTMELMRWSTLVEDYGMELRKGSLESPATDVFGSTEEGEKRWKDLKNRVVE
HNIRIMAKYYTRITMKRMAQLLDLSVDESEAFLSNLVVNKTIFAKVDRLAGIINFQRPKD
PNNLLNDWSQKLNSLMSLVNKTTHLIAKEEMIHNLQ
Sequence of entity 2 (C), FASTA
>8USB_2 26S protease regulatory subunit 8 (chains C)
MALDGPEQMELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLR
EELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDS
YTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGI
AQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMARE
HAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDI
LDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVK
GVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK
Sequence of entity 3 (b), FASTA
>8USB_3 26S proteasome non-ATPase regulatory subunit 4 (chains b)
MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEV
LTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDN
EKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLA
DALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAA
ASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEF
GQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLA
SQATKDGKKDKKEEDKK
Sequence of entity 4 (d), FASTA
>8USB_4 26S proteasome non-ATPase regulatory subunit 8 (chains d)
MFIKGRAPRAPPRERRRATRGGLRQVVAPPRALGSTSRPHFRRASVCRRRCRKSGGLLAA
SRKMAAAAVNGAAGFSSSGPAATSGAVLQAATGMYEQLKGEWNRKSPNLSKCGEELGRLK
LVLLELNFLPTTGTKLTKQQLILARDILEIGAQWSILRKDIPSFERYMAQLKCYYFDYKE
QLPESAYMHQLLGLNLLFLLSQNRVAEFHTELERLPAKDIQTNVYIKHPVSLEQYLMEGS
YNKVFLAKGNIPAESYTFFIDILLDTIRDEIAGCIEKAYEKILFTEATRILFFNTPKKMT
DYAKKRGWVLGPNNYYSFASQQQKPEDTTIPSTELAKQVIEYARQLEMIV
Sequence of entity 5 (f), FASTA
>8USB_5 26S proteasome non-ATPase regulatory subunit 2 (chains f)
MEEGGRDKAPVQPQQSPAAAPGGTDEKPSGKERRDAGDKDKEQELSEEDKQLQDELEMLV
ERLGEKDTSLYRPALEELRRQIRSSTTSMTSVPKPLKFLRPHYGKLKEIYENMAPGENKR
FAADIISVLAMTMSGERECLKYRLVGSQEELASWGHEYVRHLAGEVAKEWQELDDAEKVQ
REPLLTLVKEIVPYNMAHNAEHEACDLLMEIEQVDMLEKDIDENAYAKVCLYLTSCVNYV
PEPENSALLRCALGVFRKFSRFPEALRLALMLNDMELVEDIFTSCKDVVVQKQMAFMLGR
HGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYKTHLENNRFGG
SGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSAAASLGMILLW
DVDGGLTQIDKYLYSSEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLG
SIFGLGLAYAGSNREDVLTLLLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQ
TIMEKSETELKDTYARWLPLGLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAY
AGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALI
AMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPE
VSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTHLGKGTLTLCP
YHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPL
PVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVIL
RKNPNYDL
Sequence of entity 6 (g), FASTA
>8USB_6 NEDD8 ultimate buster 1 (chains g)
GPLGMAQKKYLQAKLTQFLREDRIQLWKPPYTDENKKVGLALKDLAKQYSDRLECCENEV
EKVIEEIRCKAIERGTGNDNYRTTGIATIEVFLPPRLKKDRKNLLETRLHITGRELRSKI
AETFGLQENYIKIVINKKQLQLGKTLEEQGVAHNVKAMVLELKQSEEDARKNFQLEEEEQ
NEAKLKEKQIQRTKRGLEILAKRAAETVVDPEMTPYLDIANQTGRSIRIPPSERKALMLA
MGYHEKGRAFLKRKEYGIALPCLLDADKYFCECCRELLDTVDNYAVLQLDIVWCYFRLEQ
LECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQGIRNYHSGND
VEAYEYLNKARQLFKELYIDPSKVDNLLQLGFTAQEARLGLRACDGNVDHAATHITNRRE
ELAQIRKEEKEKKRRRLENIRFLKGMGYSTHAAQQILLSNPQMWWLNDSNPETDNRQESP
SQENIDRLVYMGFDALVAEAALRVFRGNVQLAAQTLAHNGGSLPPELPLSPEDSLSPPAT
SPSDSAGTSSASTDEDMETEAVNEILEDIPEHEEDYLDSTLEDEEIIIAEYLSYVENRKS
ATKKN
Sequence of entity 7 (B), FASTA
>8USB_7 26S proteasome regulatory subunit 4 (chains B)
MGQSQSGGHGPGGGKKDDKDKKKKYEPPVPTRVGKKKKKTKGPDAASKLPLVTPHTQCRL
KLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNH
AIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAP
QETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVA
NQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSG
GEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKT
KKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFK
KSKENVLYKKQEGTPEGLYL
Sequence of entity 8 (A), FASTA
>8USB_8 26S proteasome regulatory subunit 7 (chains A)
MPDYLGADQRKTKEDEKDDKPIRALDEGDIALLKTYGQSTYSRQIKQVEDDIQQLLKKIN
ELTGIKESDTGLAPPALWDLAADKQTLQSEQPLQVARCTKIINADSEDPKYIINVKQFAK
FVVDLSDQVAPTDIEEGMRVGVDRNKYQIHIPLPPKIDPTVTMMQVEEKPDVTYSDVGGC
KEQIEKLREVVETPLLHPERFVNLGIEPPKGVLLFGPPGTGKTLCARAVANRTDACFIRV
IGSELVQKYVGEGARMVRELFEMARTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTML
ELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHAR
SMSVERDIRFELLARLCPNSTGAEIRSVCTEAGMFAIRARRKIATEKDFLEAVNKVIKSY
AKFSATPRYMTYN
Sequence of entity 9 (D), FASTA
>8USB_9 26S proteasome regulatory subunit 6B (chains D)
MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEY
IKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTID
RELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREA
VELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYL
GEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFD
QNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDL
EDYVARPDKISGADINSICQESGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK
Sequence of entity 10 (E), FASTA
>8USB_10 26S protease regulatory subunit 10B (chains E)
MADPRDKALQDYRKKLLEHKEIDGRLKELREQLKELTKQYEKSENDLKALQSVGQIVGEV
LKQLTEEKFIVKATNGPRYVVGCRRQLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVY
NMSHEDPGNVSYSEIGGLSEQIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGK
TLLARAVASQLDCNFLKVVSSSIVDKYIGESARLIREMFNYARDHQPCIIFMDEIDAIGG
RRFSEGTSADREIQRTLMELLNQMDGFDTLHRVKMIMATNRPDTLDPALLRPGRLDRKIH
IDLPNEQARLDILKIHAGPITKHGEIDYEAIVKLSDGFNGADLRNVCTEAGMFAIRADHD
FVVQEDFMKAVRKVADSKKLESKLDYKPV
Sequence of entity 11 (F), FASTA
>8USB_11 26S proteasome regulatory subunit 6A (chains F)
MNLLPNIESPVTRQEKMATVWDEAEQDGIGEEVLKMSTEEIIQRTRLLDSEIKIMKSEVL
RVTHELQAMKDKIKENSEKIKVNKTLPYLVSNVIELLDVDPNDQEEDGANIDLDSQRKGK
CAVIKTSTRQTYFLPVIGLVDAEKLKPGDLVGVNKDSYLILETLPTEYDSRVKAMEVDER
PTEQYSDIGGLDKQIQELVEAIVLPMNHKEKFENLGIQPPKGVLMYGPPGTGKTLLARAC
AAQTKATFLKLAGPQLVQMFIGDGAKLVRDAFALAKEKAPSIIFIDELDAIGTKRFDSEK
AGDREVQRTMLELLNQLDGFQPNTQVKVIAATNRVDILDPALLRSGRLDRKIEFPMPNEE
ARARIMQIHSRKMNVSPDVNYEELARCTDDFNGAQCKAVCVEAGMIALRRGATELTHEDY
MEGILEVQAKKKANLQYYA
Sequence of entity 12 (G), FASTA
>8USB_12 Proteasome subunit alpha type-6 (chains G)
MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKL
LDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIAD
ISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLE
KKVKKKFDWTFEQTVETAITCLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLV
ALAERD
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ZN | Zinc ion | Zn | 1 |
| ATP | Adenosine-5'-triphosphate | C10 H16 N5 O13 P3 | 5 |
| MG | Magnesium ion | Mg | 6 |
| ADP | Adenosine-5'-diphosphate | C10 H15 N5 O10 P2 | 1 |
Primary citation
NUB1 traps unfolded FAT10 for ubiquitin-independent degradation by the 26S proteasome. Arkinson, C., Dong, K.C., Gee, C.L. et al. Nat Struct Mol Biol (2025) 32:1752-1765. DOI 10.1038/s41594-025-01527-3 · PubMed
Other PDB entries of the same protein (UniProt O00232 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 9K53 2.5 Å, Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state ED2.1
- 9MBP 2.75 Å, Substrate-engaged human 26S proteasome bound to midnolin with RPT1 at top of spiral…
- 9NKG 2.8 Å, Structure of substrate engaged MIDN-bound human 26S proteasome, EB-MIDN (Composite map)
- 9E8I 2.87 Å, Human proteasome in resting state conformation bound to TXNL1 in Forward conformation
- 9BV3 2.9 Å, M1B Midnolin-Proteasome
- 9E8H 2.9 Å, Human proteasome in resting state conformation bound to TXNL1 in backward conformation
- 9K4J 2.9 Å, Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state EA1.0
- 9NKF 2.9 Å, Structure of human substrate-free 26S proteasome in the presence of ATPgS and…
- 9U3L 2.91 Å, Substrate-engaged human 26S proteasome bound to midnolin with RPT2 at top of spiral…
- 9NKI 2.94 Å, Structure of substrate engaged MIDN-bound human 26S proteasome, EB MIDN_UBL state…
- 6MSB 3.0 Å, Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome
- 7W37 3.0 Å, Structure of USP14-bound human 26S proteasome in state EA1_UBL
Browse structure collections
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