8W8Y: Gamma-MSH-MC3R-Gs_Nb35 complex
Cryo-EM structure of gamma-MSH-MC3R-Gs_Nb35 complex. Determined by electron microscopy at 2.9 Å resolution. Released 19 Mar 2025.
- Method
- Electron microscopy
- Resolution
- 2.9 Å
- Organisms
- Escherichia coli, Homo sapiens, Rattus norvegicus
- Chains
- 6
- Atoms
- 7,642
- Mol. weight
- 179.88 kDa
- Ligands
- CA
- Released
- 19 Mar 2025
Explore 8W8Y in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8W8Y contains 28 α-helices and 44 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 6 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 16-38 | 23 | |
| β-strand | 41-44 | 4 | 10 |
| β-strand | 209-214 | 6 | 10 |
| β-strand | 217-222 | 6 | 10 |
| α-helix | 235-237 | 3 | |
| β-strand | 243-248 | 6 | 10 |
| α-helix | 265-277 | 13 | |
| β-strand | 286-291 | 6 | 10 |
| α-helix | 313-317 | 5 | |
| α-helix | 332-349 | 18 | |
| β-strand | 359-363 | 5 | 10 |
| α-helix | 371-390 | 20 | |
Chain B: 4 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-24 | 21 | |
| α-helix | 30-33 | 4 | |
| α-helix | 34-36 | 3 | |
| α-helix | 38-39 | 2 | |
| β-strand | 47-51 | 5 | 1 |
| β-strand | 58-63 | 6 | 2 |
| β-strand | 69-74 | 6 | 2 |
| β-strand | 78-83 | 6 | 2 |
| β-strand | 88-94 | 7 | 2 |
| β-strand | 100-105 | 6 | 3 |
| β-strand | 111-116 | 6 | 3 |
| β-strand | 121-125 | 5 | 3 |
| β-strand | 134-139 | 6 | 3 |
| β-strand | 146-153 | 8 | 4 |
| β-strand | 156-161 | 6 | 4 |
| β-strand | 166-170 | 5 | 4 |
| β-strand | 175-180 | 6 | 4 |
| β-strand | 187-192 | 6 | 5 |
| β-strand | 198-203 | 6 | 5 |
| β-strand | 208-212 | 5 | 5 |
| β-strand | 217-222 | 6 | 5 |
| β-strand | 229-234 | 6 | 6 |
| β-strand | 240-245 | 6 | 6 |
| β-strand | 250-254 | 5 | 6 |
| β-strand | 259-264 | 6 | 6 |
| β-strand | 273-278 | 6 | 7 |
| β-strand | 284-289 | 6 | 7 |
| β-strand | 294-298 | 5 | 7 |
| β-strand | 304-308 | 5 | 7 |
| β-strand | 315-320 | 6 | 1 |
| β-strand | 327-331 | 5 | 1 |
| β-strand | 336-339 | 4 | 1 |
Chain G: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-22 | 14 | |
| α-helix | 30-43 | 14 | |
| α-helix | 45-47 | 3 | |
| α-helix | 53-55 | 3 | |
Chain N: 3 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 8 |
| β-strand | 10-11 | 2 | 9 |
| β-strand | 17-25 | 9 | 8 |
| α-helix | 29-31 | 3 | |
| β-strand | 34-39 | 6 | 9 |
| β-strand | 45-51 | 7 | 9 |
| β-strand | 58-60 | 3 | 9 |
| α-helix | 62-64 | 3 | |
| β-strand | 68-73 | 6 | 8 |
| β-strand | 78-84 | 7 | 8 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-98 | 7 | 9 |
| β-strand | 122-125 | 4 | 9 |
Chain R: 11 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 44-64 | 21 | |
| α-helix | 72-100 | 29 | |
| α-helix | 111-147 | 37 | |
| α-helix | 158-181 | 24 | |
| α-helix | 188-222 | 35 | |
| α-helix | 239-264 | 26 | |
| α-helix | 276-279 | 4 | |
| α-helix | 281-284 | 4 | |
| α-helix | 286-292 | 7 | |
| α-helix | 295-299 | 5 | |
| α-helix | 304-309 | 6 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Soluble cytochrome b562,Melanocortin receptor 3,Fusion protein | R | protein | 602 | Escherichia coli, Homo sapiens | P41968 (AlphaFold model) |
| Gama-MSH | L | protein | 11 | Homo sapiens | P01189 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 384 | Rattus norvegicus | P54311 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Bos taurus | P63212 (AlphaFold model) |
| Nanobody35 | N | protein | 160 | Lama glama | |
| Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | A | protein | 380 | Homo sapiens | P63092 |
Sequence of entity 1 (R), FASTA
>8W8Y_1 Soluble cytochrome b562,Melanocortin receptor 3,Fusion protein (chains R)
DYKDDDDKGSADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLED
KSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLLVPR
GSNASCCLPSVQPTLPNGSEHLQAPFFSNQSSSAFCEQVFIKPEVFLSLGIVSLLENILV
ILAVVRNGNLHSPMYFFLCSLAVADMLVSVSNALETIMIAIVHSDYLTFEDQFIQHMDNI
FDSMICISLVASICNLLAIAVDRYVTIFYALRYHSIMTVRKALTLIVAIWVCCGVCGVVF
IVYSESKMVIVCLITMFFAMMLLMGTLYVHMFLFARLHVKRIAALPPADGVAPQQHSCMK
GAVTITILLGVFIFCWAPFFLHLVLIITCPTNPYCICYTAHFNTYLVLIMCNSVIDPLIY
AFRSLELRNTFREILCGCNGMNLGVFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLA
VSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYG
TLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVTI
NS
Sequence of entity 2 (L), FASTA
>8W8Y_2 Gama-MSH (chains L)
YVMGHFRWDRF
Sequence of entity 3 (B), FASTA
>8W8Y_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR
IQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMT
CAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSG
DTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTF
TGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSG
RLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGS
SGGGGSGGGGSSGVSGWRLFKKIS
Sequence of entity 4 (G), FASTA
>8W8Y_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 5 (N), FASTA
>8W8Y_5 Nanobody35 (chains N)
MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVR
QAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCAR
CPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHHEPEA
Sequence of entity 6 (A), FASTA
>8W8Y_6 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQM
RILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSV
MNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSD
QDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYN
MVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPE
FARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRR
VFNDCRDIIQRMHLRQYELL
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| CA | Calcium ion | Ca | 1 |
Primary citation
Structure basis of ligand selectivity and signal transduction in human melanocortin-3 receptor. Jingpeng, F., Hongli, H. To be published.
Other PDB entries of the same protein (UniProt P41968 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 9K3F 2.75 Å, Cryo-EM structure of the unliganded human melanocortin receptor 3 (MC3R)-Gs complex
- 8IOC 2.86 Å, Cryo-EM structure of the gamma-MSH-bound human melanocortin receptor 3 (MC3R)-Gs complex
- 8W8W 2.9 Å, Cryo-EM structure of alpha-MSH-MC3R-Gs_Nb35 complex
- 8W8X 2.9 Å, Cryo-EM structure of beta-MSH-MC3R-Gs_Nb35 complex
- 8KIG 3.1 Å, Cryo-EM structure of MC3R in complex with SHU9119
Browse structure collections
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