8Y0C: FnCas12a

Crystal structure of FnCas12a in complex with pre-crRNA and 18nt target DNA. Determined by X-ray diffraction at 3.45 Å resolution. Released 1 Jan 2025.

Method

X-ray diffraction

Resolution

3.45 Å

Organism

Francisella tularensis subsp. novicida U112

Chains

4

Atoms

12,057

Mol. weight

182.27 kDa

Ligands

MG

Released

1 Jan 2025

Explore 8Y0C in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8Y0C contains 70 α-helices and 48 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 70 helices, 48 β-strands

Molecules and chains

>8Y0C_1 RNA (42-MER) (chains B)
GGAAUUUCUACUGUUGUAGAUGAGAAGUCAUUUAAUAAGGCCGUCUAAGAACUUUAUC

>8Y0C_2 DNA (27-MER) (chains C)
CTTATTAAATGACTTCTCTAAAGGACT

>8Y0C_3 DNA (5'-D(P*AP*GP*TP*CP*CP*TP*TP*TP*AP*CP*T)-3') (chains D)
AGTCCTTTACT

>8Y0C_4 CRISPR-associated endonuclease Cas12a (chains A)
MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKKAKQIIDKYHQF
FIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDTIKKQISEYIKDSEKFK
NLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDIDEALEIIKSFKGWTTYFK
GFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAE
ELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGI
NEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDKLEDDSDVVTTMQSFYEQIA
AFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQVFDDYSVIGTAVLEY
ITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKLALEEFNKHRDIDKQCRFEEILA
NFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQASAEDDVKAIKDLLDQTNNLLHKL
KIFHISQSEDKANILDKDEHFYLVFEECYFELANIVPLYNKIRNYITQKPYSDEKFKLNF
ENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVYK
LLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEKFEFNIEDCRKF
IDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKLTFENISESYIDSVVNQ
GKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFDERNLQDVVYKLNGEAELFYRKQSIPKK
ITHPAKEAIANKNKDNPKKESVFEYDLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEI
NLLLKEKANDVHILSIDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAAI
EKDRDSARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFADLNFGFKRGRFKVE
KQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQTGIIYYVPAG
FTSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKG
KWTIASFGSRLINFRNSDKNHNWDTREVYPTKELEKLLKDYSIEYGHGECIKAAICGESD
KKFFAKLTSVLNTILQMRNSKTGTELDYLISPVADVNGNFFDSRQAPKNMPQDADANGAY
HIGLKGLMLLGRIKNNQEGKKLNLVIKNEEYFEFVQNRNN

Ligands and cofactors

Primary citation

DNA target binding-induced pre-crRNA processing in type II and V CRISPR-Cas systems. Chen, J., Lin, X., Xiang, W. et al. Nucleic Acids Res (2025) 53. DOI 10.1093/nar/gkae1241 · PubMed

Related structures

Other PDB entries of the same protein (UniProt A0Q7Q2 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 8Y0B 2.3 Å, Crystal structure of FnCas12a in complex with pre-crRNA and 12nt target DNA
• 5NFV 2.5 Å, Crystal structure of catalytically inactive FnCas12 mutant bound to an R-loop structure…
• 6I1K 2.65 Å, Crystal structure of catalytically inactive FnCas12a in complex with a crRNA guide and a…
• 6I1L 2.98 Å, Crystal structure of FnCas12a in complex with a crRNA guide and ssDNA target
• 5MGA 3.0 Å, Structure of the Cpf1 endonuclease R-loop complex after DNA cleavage
• 9MKX 3.21 Å, FnoCas12a bridge helix variant state 4b
• 9MKT 3.26 Å, FnoCas12a bridge helix variant state 1
• 6GTG 3.27 Å, Transition state structure of Cpf1(Cas12a) I4 conformation
• 5NG6 3.34 Å, Crystal structure of FnCas12a bound to a crRNA
• 6GTF 3.63 Å, Transient state structure of CRISPR-Cpf1 (Cas12a) I5 conformation
• 9MKW 3.63 Å, FnoCas12a bridge helix variant state 4a
• 9MKV 3.68 Å, FnoCas12a bridge helix variant state 3

Browse structure collections

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