8YK0: Human GPR156-Gi3 complex
Cryo-EM structure of human GPR156-Gi3 complex. Determined by electron microscopy at 2.4 Å resolution. Released 5 Feb 2025.
- Method
- Electron microscopy
- Resolution
- 2.4 Å
- Organisms
- Homo sapiens, Rattus norvegicus, Bos taurus
- Chains
- 5
- Atoms
- 9,831
- Mol. weight
- 158.81 kDa
- Ligands
- CLR, MW9
- Released
- 5 Feb 2025
Explore 8YK0 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8YK0 contains 40 α-helices and 40 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 11 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 24-33 | 10 | |
| α-helix | 47-73 | 27 | |
| α-helix | 78-82 | 5 | |
| α-helix | 85-104 | 20 | |
| α-helix | 113-150 | 38 | |
| α-helix | 162-186 | 25 | |
| β-strand | 191-201 | 11 | 1 |
| β-strand | 206-216 | 11 | 1 |
| α-helix | 221-245 | 25 | |
| α-helix | 252-255 | 4 | |
| α-helix | 257-280 | 24 | |
| α-helix | 285-315 | 31 | |
| α-helix | 316-318 | 3 | |
Chain B: 10 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 23-33 | 11 | |
| α-helix | 47-73 | 27 | |
| α-helix | 78-82 | 5 | |
| α-helix | 85-104 | 20 | |
| α-helix | 115-148 | 34 | |
| α-helix | 162-186 | 25 | |
| β-strand | 190-201 | 12 | 1 |
| β-strand | 206-217 | 12 | 1 |
| α-helix | 221-245 | 25 | |
| α-helix | 252-255 | 4 | |
| α-helix | 257-280 | 24 | |
| α-helix | 285-318 | 34 | |
Chain C: 10 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-30 | 24 | |
| β-strand | 33-40 | 8 | 9 |
| α-helix | 46-55 | 10 | |
| β-strand | 185-191 | 7 | 9 |
| β-strand | 194-200 | 7 | 9 |
| α-helix | 208-211 | 4 | |
| β-strand | 220-226 | 7 | 9 |
| α-helix | 227-231 | 5 | |
| β-strand | 233 | 1 | 10 |
| β-strand | 241 | 1 | 10 |
| α-helix | 242-254 | 13 | |
| α-helix | 257-259 | 3 | |
| β-strand | 263-269 | 7 | 9 |
| α-helix | 271-277 | 7 | |
| α-helix | 283-285 | 3 | |
| α-helix | 296-309 | 14 | |
| β-strand | 319-323 | 5 | 9 |
| α-helix | 331-351 | 21 | |
Chain D: 5 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-25 | 21 | |
| α-helix | 30-33 | 4 | |
| α-helix | 38-39 | 2 | |
| β-strand | 47-51 | 5 | 2 |
| β-strand | 58-63 | 6 | 3 |
| β-strand | 69-74 | 6 | 3 |
| β-strand | 78-83 | 6 | 3 |
| β-strand | 88-94 | 7 | 3 |
| α-helix | 95 | 1 | |
| β-strand | 100-105 | 6 | 4 |
| β-strand | 111-116 | 6 | 4 |
| β-strand | 121-125 | 5 | 4 |
| β-strand | 134-139 | 6 | 4 |
| β-strand | 146-151 | 6 | 5 |
| β-strand | 156-161 | 6 | 5 |
| β-strand | 166-170 | 5 | 5 |
| β-strand | 175-180 | 6 | 5 |
| β-strand | 189-192 | 4 | 6 |
| β-strand | 198-202 | 5 | 6 |
| β-strand | 208-212 | 5 | 6 |
| β-strand | 218-222 | 5 | 6 |
| β-strand | 229-234 | 6 | 7 |
| β-strand | 240-245 | 6 | 7 |
| β-strand | 249-254 | 6 | 7 |
| β-strand | 259-265 | 7 | 7 |
| α-helix | 272 | 1 | |
| β-strand | 273-278 | 6 | 8 |
| β-strand | 284-289 | 6 | 8 |
| β-strand | 294-298 | 5 | 8 |
| β-strand | 304-308 | 5 | 8 |
| β-strand | 315-320 | 6 | 2 |
| β-strand | 327-331 | 5 | 2 |
| β-strand | 336-339 | 4 | 2 |
Chain G: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-24 | 15 | |
| α-helix | 30-43 | 14 | |
| α-helix | 53-55 | 3 | |
| α-helix | 56-58 | 3 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Probable G-protein coupled receptor 156 | B | protein | 298 | Homo sapiens | Q8NFN8 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | D | protein | 338 | Rattus norvegicus | P54311 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 57 | Bos taurus | P63212 (AlphaFold model) |
| Guanine nucleotide-binding protein G(i) subunit alpha-3 | C | protein | 349 | Homo sapiens | P08754 (AlphaFold model) |
| Probable G-protein coupled receptor 156 | A | protein | 317 | Homo sapiens | Q8NFN8 (AlphaFold model) |
Sequence of entity 1 (B), FASTA
>8YK0_1 Probable G-protein coupled receptor 156 (chains B)
RPLHDLCKTTITSSHHSSKTISSLSPVLLGIVWTFLSCGLLLILFFLAFTIHCRKNRIVK
MSSPNLNIVTLLGSCLTYSSAYLFGIQDVLVGSSMETLIQTRLSMLCIGTSLVFGPILGK
SWRLYKVFTQRVPDKRVIIKDLQLLGLVAALLMADVILLMTWVLTDPIQCLQILSVSMTV
TGKDVSCTSTSTHFCASRYSDVWIALIWGCKGLLLLYGAYLAGLTGHVSSPPVNQSLTIM
VGVNLLVLAAGLLFVVTRYLHSWPNLVFGLTSGGIFVCTTTINCFIFIPQLKQWKAFE
Sequence of entity 2 (D), FASTA
>8YK0_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains D)
ELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMH
WGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICS
IYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTG
HTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFA
TGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKA
DRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 3 (G), FASTA
>8YK0_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
ASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFRE
Sequence of entity 4 (C), FASTA
>8YK0_4 Guanine nucleotide-binding protein G(i) subunit alpha-3 (chains C)
SAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKNTIVKQMKIIHEDGYSEDE
CKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAG
VIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIV
ETHFTFKDLYFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHA
SMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQC
QFEDLNRRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKECGLY
Sequence of entity 5 (A), FASTA
>8YK0_5 Probable G-protein coupled receptor 156 (chains A)
RPLHDLCKTTITSSHHSSKTISSLSPVLLGIVWTFLSCGLLLILFFLAFTIHCRKNRIVK
MSSPNLNIVTLLGSCLTYSSAYLFGIQDVLVGSSMETLIQTRLSMLCIGTSLVFGPILGK
SWRLYKVFTQRVPDKRVIIKDLQLLGLVAALLMADVILLMTWVLTDPIQCLQILSVSMTV
TGKDVSCTSTSTHFCASRYSDVWIALIWGCKGLLLLYGAYLAGLTGHVSSPPVNQSLTIM
VGVNLLVLAAGLLFVVTRYLHSWPNLVFGLTSGGIFVCTTTINCFIFIPQLKQWKAFEEE
NQTIRRMAKYFSTPNKS
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| CLR | Cholesterol | C27 H46 O | 11 |
| MW9 | (21R,24R,27S)-24,27,28-trihydroxy-18,24-dioxo-19,23,25-trioxa-24lambda~5~-phosp… | C42 H81 O10 P | 4 |
Primary citation
Molecular insights into the activation mechanism of GPR156 in maintaining auditory function. Ma, X., Chen, L.N., Liao, M. et al. Nat Commun (2024) 15:10601-10601. DOI 10.1038/s41467-024-54681-5 · PubMed
Other PDB entries of the same protein (UniProt Q8NFN8 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8IEP 2.61 Å, Cryo-EM structure of GPR156C/D of G-protein free GPR156 (local refine)
- 8IEI 2.62 Å, Cryo-EM structure of GPR156A/B of G-protein free GPR156 (local refine)
- 8IEQ 2.73 Å, Cryo-EM structure of G-protein free GPR156
- 8IEB 3.03 Å, Cryo-EM structure of GPR156 of GPR156-miniGo-scFv16 complex (local refine)
- 8YJP 3.09 Å, Cryo-EM structure of human apo GPR156
- 8IED 3.33 Å, Cryo-EM structure of GPR156-miniGo-scFv16 complex
Browse structure collections
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