8YW8: Human mitochondrial pyruvate carrier

Cryo-EM structure of human mitochondrial pyruvate carrier in complex with the inhibitor UK5099. Determined by electron microscopy at 3.17 Å resolution. Released 12 Mar 2025.

Method: Electron microscopy
Resolution: 3.17 Å
Organisms: Homo sapiens, Escherichia coli BL21(DE3)
Chains: 3
Atoms: 2,697
Mol. weight: 47.76 kDa
Ligands: I2R, CDL
Released: 12 Mar 2025

Explore 8YW8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8YW8 contains 18 α-helices and 11 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 0 β-strands

Element	Residues	Length
α-helix	25-33	9
α-helix	35-42	8
α-helix	43-45	3
α-helix	48-50	3
α-helix	53-72	20
α-helix	78-107	30

Chain B: 9 helices, 0 β-strands

Element	Residues	Length
α-helix	3-23	21
α-helix	26-28	3
α-helix	29-32	4
α-helix	41-48	8
α-helix	50-58	9
α-helix	59-61	3
α-helix	64-66	3
α-helix	69-88	20
α-helix	94-125	32

Chain C: 3 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	1
β-strand	10-12	3	2
β-strand	18-25	8	1
β-strand	34-39	6	2
β-strand	46-51	6	2
β-strand	58-60	3	2
α-helix	62-64	3
β-strand	68-73	6	1
α-helix	74-76	3
β-strand	78-83	6	1
α-helix	88-90	3
β-strand	92-98	7	2
β-strand	104-105	2	2
β-strand	109-113	5	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Mitochondrial pyruvate carrier 2	B	protein	151	Homo sapiens	O95563 (AlphaFold model)
MPC specific nanobody 1	C	protein	138	Escherichia coli BL21(DE3)
Mitochondrial pyruvate carrier 1	A	protein	120	Homo sapiens	Q9Y5U8 (AlphaFold model)

Sequence of entity 1 (B), FASTA

>8YW8_1 Mitochondrial pyruvate carrier 2 (chains B)
MSAAGARGLRATYHRLLDKVELMLPEKLRPLYNHPAGPRTVFFWAPIMKWGLVCAGLADM
ARPAEKLSTAQSAVLMATGFIWSRYSLVIIPKNWSLFAVNFFVGAAGASQLFRIWRYNQE
LKAKAHKGSDYKDHDGDYKDHDIDYKDDDDK

Sequence of entity 2 (C), FASTA

>8YW8_2 MPC specific nanobody 1 (chains C)
EVQLVESGGGLVQAGGSLRLSCAASGFPVTERVMYWYRQAPGKEREWVAAIDSQGSSTYY
ADSVKGRFTISRDNSKNTVYLQMNSLKPEDTAVYYCKVEVGWGYKGQGTQVTVSSLEHHH
HHHHGGSGEQKLISEEDL

Sequence of entity 3 (A), FASTA

>8YW8_3 Mitochondrial pyruvate carrier 1 (chains A)
MAGALVRKAADYVRSKDFRDYLMSTHFWGPVANWGLPIAAINDMKKSPEIISGRMTFALC
CYSLTFMRFAYKVQPRNWLLFACHATNEVAQLIQGGRLIKHEMTKTASAGSYPYDVPDYA

Ligands and cofactors

ID	Name	Formula	Copies
I2R	(E)-2-cyano-3-(1-phenylindol-3-yl)prop-2-enoic acid	C18 H12 N2 O2	1
CDL	Cardiolipin	C81 H156 O17 P2	1

Primary citation

Structures and mechanism of the human mitochondrial pyruvate carrier. Liang, J., Shi, J., Song, A. et al. Nature (2025) 641:258-265. DOI 10.1038/s41586-025-08873-8 · PubMed

Other PDB entries of the same protein (UniProt O95563 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9MNZ 2.73 Å, Cryo-EM structure of human MPC in complex with UK5099 in nanodiscs
9MNY 2.78 Å, Cryo-EM structure of human MPC with pyruvate
9MO0 2.83 Å, Cryo-EM structure of human MPC in complex with AKOS005153046
8YW9 3.01 Å, Cryo-EM structure of human mitochondrial pyruvate carrier in the matrix-facing…
9MNX 3.11 Å, Cryo-EM structure of human MPC in complex with UK5099 in LMNG
8YW6 3.18 Å, Cryo-EM structure of apo human mitochondrial pyruvate carrier in the IMS-open…
9O9T 3.31 Å, Structure of human MPC IMS-open
9MNW 3.35 Å, Cryo-EM structure of human MPC in complex with GW604714
9KNY 3.4 Å, Cryo-EM structure of pyruvate-treated human mitochondrial pyruvate carrier in the…
9KNW 3.41 Å, Cryo-EM structure of apo human mitochondrial pyruvate carrier in the IMS-open…
9O9S 3.57 Å, Structure of human MPC matrix-open
9GIV 3.65 Å, Structure of the human mitochondrial pyruvate carrier inhibited by a UK5099-derivative

Browse structure collections

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