8ZH8: Human GPR103 -Gq complex

Human GPR103 -Gq complex bound to QRFP26. Determined by electron microscopy at 3.19 Å resolution. Released 3 Jul 2024.

Method: Electron microscopy
Resolution: 3.19 Å
Organisms: Homo sapiens, Lama glama, Rattus norvegicus
Chains: 7
Atoms: 10,048
Mol. weight: 174.09 kDa
Released: 3 Jul 2024

Explore 8ZH8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8ZH8 contains 40 α-helices and 74 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	33-39	7	12
β-strand	69	1	13
β-strand	72-76	5	12
β-strand	79-84	6	12
β-strand	86	1	13
β-strand	105-111	7	12
α-helix	115-129	15
β-strand	138-144	7	12
α-helix	146-155	10
α-helix	160-163	4
α-helix	165-169	5
α-helix	184-203	20
β-strand	211-215	5	12
α-helix	224-242	19

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	5-24	20
α-helix	30-33	4
α-helix	34-36	3
β-strand	47-51	5	5
β-strand	58-63	6	6
β-strand	69-74	6	6
β-strand	78-83	6	6
β-strand	88-94	7	6
β-strand	100-105	6	7
β-strand	111-116	6	7
β-strand	121-125	5	7
α-helix	129-131	3
β-strand	134-139	6	7
β-strand	146-151	6	8
β-strand	156-161	6	8
β-strand	165-170	6	8
β-strand	175-181	7	8
β-strand	187-192	6	9
β-strand	198-203	6	9
β-strand	207-212	6	9
β-strand	218-223	6	9
β-strand	229-234	6	10
β-strand	240-245	6	10
β-strand	250-254	5	10
β-strand	260-264	5	10
β-strand	273-278	6	11
β-strand	284-289	6	11
β-strand	294-298	5	11
β-strand	304-308	5	11
β-strand	315-320	6	5
β-strand	327-331	5	5
β-strand	336-339	4	5

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	12-23	12
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chain N: 2 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	2
β-strand	11-12	2	3
β-strand	18-25	8	2
α-helix	29-31	3
β-strand	34-39	6	4
β-strand	45-51	7	4
β-strand	58-60	3	4
β-strand	68-73	6	2
β-strand	78-83	6	2
α-helix	88-90	3
β-strand	92-98	7	4
β-strand	118	1	4
β-strand	122-124	3	4
β-strand	125-126	2	3

Chain Q: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	5-13	9

Chain R: 19 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	8-17	10
α-helix	22-29	8
α-helix	42-72	31
α-helix	74-76	3
α-helix	79-97	19
α-helix	99-106	8
α-helix	114-148	35
α-helix	152-154	3
α-helix	159-176	18
α-helix	179-183	5
β-strand	184-191	8	1
α-helix	193-195	3
β-strand	196-203	8	1
α-helix	208-219	12
α-helix	220-224	5
α-helix	225-242	18
α-helix	264-298	35
α-helix	301-304	4
α-helix	307-322	16
α-helix	324-334	11
α-helix	337-345	9

Chain S: 3 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	14
β-strand	10-12	3	15
β-strand	17-25	9	14
α-helix	29-31	3
β-strand	33-39	7	15
β-strand	45-51	7	15
β-strand	58-60	3	15
β-strand	68-73	6	14
β-strand	78-84	7	14
α-helix	88-90	3
β-strand	92-99	8	15
β-strand	113	1	14
β-strand	115-119	5	15
β-strand	128-129	2	16
β-strand	134-136	3	17
β-strand	143-149	7	16
β-strand	154	1	18
β-strand	160	1	18
β-strand	162-167	6	17
β-strand	174-178	5	17
β-strand	182-183	2	17
β-strand	191-196	6	16
β-strand	199-204	6	16
β-strand	214-219	6	17
α-helix	225	1
β-strand	227	1	17
β-strand	231-234	4	17

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Pyroglutamylated RF-amide peptide receptor	R	protein	402	Homo sapiens	Q96P65 (AlphaFold model)
nanobody Nb35	N	protein	137	Lama glama
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	68	Bos taurus	P63212 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2,Guanine nucleotide-binding…	A	protein	326	Homo sapiens	P04899 (AlphaFold model), P59768, Q5JWF2
scFv16	S	protein	260	Mus musculus
QRF-amide	Q	protein	27	Homo sapiens	P83859

Sequence of entity 1 (R), FASTA

>8ZH8_1 Pyroglutamylated RF-amide peptide receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDKQALNITPEQFSRLLRDHNLTREQFIALYRLRPLVYT
PELPGRAKLALVLTGVLIFALALFGNALVFYVVTRSKAMRTVTNIFICSLALSDLLITFF
CIPVTMLQNISDNWLGGAFICKMVPFVQSTAVVTEILTMTCIAVERHQGLVHPFKMKWQY
TNRRAFTMLGVVWLVAVIVGSPMWHVQQLEIKYDFLYEKEHICCLEEWTSPVHQKIYTTF
ILVILFLLPLMVMLILYSKIGYELWIKKRVGDGSVLRTIHGKEMSKIARKKKRAVIMMVT
VVALFAVCWAPFHVVHMMIEYSNFEKEYDDVTIKMIFAIVQIIGFSNSICNPIVYAFMNE
NFKKNVLSAVCYCIVNKTFSPAQRHGNSGSGGGGSGGSSSGG

Sequence of entity 2 (N), FASTA

>8ZH8_2 nanobody Nb35 (chains N)
MGQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASI
SYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYR
GQGTQVTVSSLHHHHHH

Sequence of entity 3 (B), FASTA

>8ZH8_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (G), FASTA

>8ZH8_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFS

Sequence of entity 5 (A), FASTA

>8ZH8_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2,Guanine nucleotide-binding protein G(i) subunit alpha-2,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas (chains A)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAILGSAGSAGSAMGSTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLG
ADNSGKSTIVKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCF
NDVTAIIFVVDSSDYNRLQEALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKI
EDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVD
TENARRIFNDCKDIILQMNLREYNLV

Sequence of entity 6 (S), FASTA

>8ZH8_6 scFv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAASSEDLYFQ

Sequence of entity 7 (Q), FASTA

>8ZH8_7 QRF-amide (chains Q)
TSGPLGNLAEELNGYSRKKGGFSFRFX

Primary citation

Structure and dynamics of the pyroglutamylated RF-amide peptide QRFP receptor GPR103. Iwama, A., Kise, R., Akasaka, H. et al. Nat Commun (2024) 15:4769-4769. DOI 10.1038/s41467-024-49030-5 · PubMed

Other PDB entries of the same protein (UniProt Q96P65 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8WZ2 2.73 Å, Structure of 26RFa-pyroglutamylated RFamide peptide receptor complex

Browse structure collections

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