8ZJC: Saccharomyces cerevisiae bc1 complex
Cryo-EM structure of Saccharomyces cerevisiae bc1 complex. Determined by electron microscopy at 2.5 Å resolution. Released 21 May 2025.
- Method
- Electron microscopy
- Resolution
- 2.5 Å
- Organism
- Saccharomyces cerevisiae
- Chains
- 20
- Atoms
- 32,311
- Mol. weight
- 459.9 kDa
- Ligands
- UQ6, 8PE, 9PE, HEM
- Released
- 21 May 2025
Explore 8ZJC in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8ZJC contains 227 α-helices and 114 β-strands across 20 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chains A and L: 24 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 29-33 | 5 | 1 |
| β-strand | 37-42 | 6 | 1 |
| β-strand | 49-55 | 7 | 1 |
| α-helix | 59-61 | 3 | |
| α-helix | 69-77 | 9 | |
| α-helix | 80-87 | 8 | |
| β-strand | 92-97 | 6 | 1 |
| β-strand | 102-108 | 7 | 1 |
| α-helix | 110-112 | 3 | |
| α-helix | 113-120 | 8 | |
| α-helix | 121-125 | 5 | |
| α-helix | 134-154 | 21 | |
| α-helix | 156-168 | 13 | |
| α-helix | 173-175 | 3 | |
| α-helix | 182-185 | 4 | |
| α-helix | 190-200 | 11 | |
| α-helix | 203-205 | 3 | |
| β-strand | 206-212 | 7 | 1 |
| α-helix | 216-224 | 9 | |
| α-helix | 240-242 | 3 | |
| β-strand | 247-253 | 7 | 2 |
| β-strand | 259-266 | 8 | 2 |
| α-helix | 275-282 | 8 | |
| β-strand | 287-289 | 3 | 2 |
| α-helix | 294-297 | 4 | |
| α-helix | 303-306 | 4 | |
| β-strand | 314-321 | 8 | 2 |
| β-strand | 326-334 | 9 | 2 |
| α-helix | 340-356 | 17 | |
| α-helix | 360-378 | 19 | |
| α-helix | 383-397 | 15 | |
| α-helix | 403-410 | 8 | |
| α-helix | 415-425 | 11 | |
| β-strand | 432-437 | 6 | 2 |
| α-helix | 445-449 | 5 | |
| α-helix | 450-452 | 3 | |
Chains B and M: 22 helices, 14 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 18-22 | 5 | 3 |
| β-strand | 28-35 | 8 | 3 |
| α-helix | 39-41 | 3 | |
| α-helix | 47-52 | 6 | |
| β-strand | 59 | 1 | 4 |
| α-helix | 64-74 | 11 | |
| β-strand | 76-82 | 7 | 3 |
| β-strand | 87-94 | 8 | 3 |
| α-helix | 95-97 | 3 | |
| α-helix | 98-110 | 13 | |
| β-strand | 112 | 1 | 4 |
| α-helix | 116-118 | 3 | |
| α-helix | 119-123 | 5 | |
| α-helix | 124-134 | 11 | |
| α-helix | 138-150 | 13 | |
| α-helix | 169-179 | 11 | |
| α-helix | 182-184 | 3 | |
| β-strand | 185-190 | 6 | 3 |
| α-helix | 194-202 | 9 | |
| α-helix | 205-208 | 4 | |
| α-helix | 220-222 | 3 | |
| β-strand | 228-232 | 5 | 5 |
| β-strand | 237-245 | 9 | 5 |
| α-helix | 247-249 | 3 | |
| α-helix | 250-261 | 12 | |
| α-helix | 267-270 | 4 | |
| β-strand | 273-279 | 7 | 5 |
| β-strand | 282-291 | 10 | 5 |
| α-helix | 294-310 | 17 | |
| β-strand | 312-313 | 2 | 6 |
| α-helix | 315-317 | 3 | |
| α-helix | 318-327 | 10 | |
| α-helix | 340-342 | 3 | |
| β-strand | 345-346 | 2 | 6 |
| β-strand | 352-357 | 6 | 5 |
| α-helix | 365-367 | 3 | |
Chain C: 25 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-6 | 4 | |
| α-helix | 10-13 | 4 | |
| α-helix | 14-18 | 5 | |
| β-strand | 22-23 | 2 | 7 |
| α-helix | 28-31 | 4 | |
| α-helix | 32-51 | 20 | |
| α-helix | 61-70 | 10 | |
| α-helix | 75-103 | 29 | |
| α-helix | 111-134 | 24 | |
| β-strand | 137 | 1 | 8 |
| α-helix | 138-148 | 11 | |
| α-helix | 149-152 | 4 | |
| α-helix | 158-166 | 9 | |
| α-helix | 173-202 | 30 | |
| β-strand | 218-219 | 2 | 7 |
| α-helix | 226-246 | 21 | |
| α-helix | 254-257 | 4 | |
| β-strand | 259 | 1 | 8 |
| α-helix | 260 | 1 | |
| α-helix | 273-275 | 3 | |
| α-helix | 276-282 | 7 | |
| α-helix | 288-300 | 13 | |
| α-helix | 301-304 | 4 | |
| α-helix | 305-308 | 4 | |
| β-strand | 313 | 1 | 9 |
| α-helix | 321-339 | 19 | |
| α-helix | 350-355 | 6 | |
| α-helix | 357-361 | 5 | |
| α-helix | 362-366 | 5 | |
| α-helix | 367-382 | 16 | |
Chains D and O: 16 helices, 9 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 64-67 | 4 | |
| α-helix | 69-71 | 3 | |
| α-helix | 87-99 | 13 | |
| α-helix | 101-103 | 3 | |
| β-strand | 111 | 1 | 10 |
| α-helix | 112-115 | 4 | |
| β-strand | 116 | 1 | 11 |
| β-strand | 120 | 1 | 11 |
| α-helix | 122-129 | 8 | |
| β-strand | 133-136 | 4 | 12 |
| β-strand | 145-148 | 4 | 12 |
| β-strand | 154 | 1 | 10 |
| α-helix | 155-157 | 3 | |
| α-helix | 162-167 | 6 | |
| α-helix | 174-176 | 3 | |
| α-helix | 188-195 | 8 | |
| α-helix | 202-203 | 2 | |
| α-helix | 208-209 | 2 | |
| β-strand | 213-214 | 2 | 13 |
| β-strand | 222-223 | 2 | 13 |
| α-helix | 244-259 | 16 | |
| α-helix | 263-291 | 29 | |
| α-helix | 293-296 | 4 | |
| β-strand | 299-302 | 4 | 2 |
| α-helix | 304-306 | 3 | |
Chain E: 6 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 43 | 1 | 14 |
| α-helix | 49-51 | 3 | |
| α-helix | 56-79 | 24 | |
| α-helix | 80-82 | 3 | |
| α-helix | 84-85 | 2 | |
| α-helix | 86-90 | 5 | |
| β-strand | 94 | 1 | 15 |
| β-strand | 108-109 | 2 | 16 |
| β-strand | 116-120 | 5 | 16 |
| α-helix | 143-146 | 4 | |
| β-strand | 152-155 | 4 | 16 |
| β-strand | 176-178 | 3 | 17 |
| β-strand | 183-185 | 3 | 17 |
| β-strand | 206-207 | 2 | 18 |
| β-strand | 212-213 | 2 | 18 |
| β-strand | 214 | 1 | 15 |
Chain F: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 79-82 | 4 | |
| α-helix | 85-87 | 3 | |
| α-helix | 90-108 | 19 | |
| α-helix | 124-142 | 19 | |
Chain G: 9 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-16 | 12 | |
| α-helix | 21-23 | 3 | |
| α-helix | 26-34 | 9 | |
| α-helix | 39-41 | 3 | |
| α-helix | 45-47 | 3 | |
| β-strand | 49 | 1 | 9 |
| α-helix | 54-61 | 8 | |
| α-helix | 65-83 | 19 | |
| α-helix | 94-95 | 2 | |
| α-helix | 104-121 | 18 | |
| β-strand | 123 | 1 | 19 |
Chains H and S: 3 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 12 | 1 | 20 |
| β-strand | 15 | 1 | 20 |
| β-strand | 22-29 | 8 | 2 |
| β-strand | 34 | 1 | 14 |
| α-helix | 46-55 | 10 | |
| α-helix | 57-80 | 24 | |
| α-helix | 86-93 | 8 | |
8 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| COR1 isoform 1 | A, L | protein | 431 | Saccharomyces cerevisiae | P07256 (AlphaFold model) |
| Cytochrome b-c1 complex subunit 2, mitochondrial | B, M | protein | 352 | Saccharomyces cerevisiae | P07257 (AlphaFold model) |
| Cytochrome b | C, N | protein | 385 | Saccharomyces cerevisiae | P00163 (AlphaFold model) |
| Cytochrome c1, heme protein, mitochondrial | D, O | protein | 248 | Saccharomyces cerevisiae | P07143 (AlphaFold model) |
| Cytochrome b-c1 complex subunit Rieske, mitochondrial | E, P | protein | 185 | Saccharomyces cerevisiae | P08067 |
| Cytochrome b-c1 complex subunit 6, mitochondrial | F, Q | protein | 75 | Saccharomyces cerevisiae | P00127 |
| Cytochrome b-c1 complex subunit 7 | G, R | protein | 126 | Saccharomyces cerevisiae | P00128 |
| Cytochrome b-c1 complex subunit 8 | H, S | protein | 93 | Saccharomyces cerevisiae | P08525 |
| Cytochrome b-c1 complex subunit 9, mitochondrial | I, T | protein | 55 | Saccharomyces cerevisiae | P22289 |
| Cytochrome b-c1 complex subunit 10, mitochondrial | U, V | protein | 52 | Saccharomyces cerevisiae | P37299 |
Sequence of entity 1 (A, L), FASTA
>8ZJC_1 COR1 isoform 1 (chains A, L)
AEVTQLSNGIVVATEHNPSAHTASVGVVFGSGAANENPYNNGVSNLWKNIFLSKENSAVA
AKEGLALSSNISRDFQSYIVSSLPGSTDKSLDFLNQSFIQQKANLLSSSNFEATKKSVLK
QVQDFEENDHPNRVLEHLHSTAFQNTPLSLPTRGTLESLENLVVADLESFANNHFLNSNA
VVVGTGNIKHEDLVNSIESKNLSLQTGTKPVLKKKAAFLGSEVRLRDDTLPKAWISLAVE
GEPVNSPNYFVAKLAAQIFGSYNAFEPASRLQGIKLLDNIQEYQLCDNFNHFSLSYKDSG
LWGFSTATRNVTMIDDLIHFTLKQWNRLTISVTDTEVERAKSLLKLQLGQLYESGNPVND
ANLLGAEVLIKGSKLSLGEAFKKIDAITVKDVKAWAGKRLWDQDIAIAGTGQIEGLLDYM
RIRSDMSMMRW
Sequence of entity 2 (B, M), FASTA
>8ZJC_2 Cytochrome b-c1 complex subunit 2, mitochondrial (chains B, M)
LTVSARDAPTKISTLAVKVHGGSRYATKDGVAHLLNRFNFQNTNTRSALKLVRESELLGG
TFKSTLDREYITLKATFLKDDLPYYVNALADVLYKTAFKPHELTESVLPAARYDYAVAEQ
CPVKSAEDQLYAITFRKGLGNPLLYDGVERVSLQDIKDFADKVYTKENLEVSGENVVEAD
LKRFVDESLLSTLPAGKSLVSKSEPKFFLGEENRVRFIGDSVAAIGIPVNKASLAQYEVL
ANYLTSALSELSGLISSAKLDKFTDGGLFTLFVRDQDSAVVSSNIKKIVADLKKGKDLSP
AINYTKLKNAVQNESVSSPIELNFDAVKDFKLGKFNYVAVGDVSNLPYLDEL
Sequence of entity 3 (C, N), FASTA
>8ZJC_3 Cytochrome b (chains C, N)
MAFRKSNVYLSLVNSYIIDSPQPSSINYWWNMGSLLGLCLVIQIVTGIFMAMHYSSNIEL
AFSSVEHIMRDVHNGYILRYLHANGASFFFMVMFMHMAKGLYYGSYRSPRVTLWNVGVII
FILTIATAFLGYCCVYGQMSHWGATVITNLFSAIPFVGNDIVSWLWGGFSVSNPTIQRFF
ALHYLVPFIIAAMVIMHLMALHIHGSSNPLGITGNLDRIPMHSYFIFKDLVTVFLFMLIL
ALFVFYSPNTLGHPDNYIPGNPLVTPASIVPEWYLLPFYAILRSIPDKLLGVITMFAAIL
VLLVLPFTDRSVVRGNTFKVLSKFFFFIFVFNFVLLGQIGACHVEVPYVLMGQIATFIYF
AYFLIIVPVISTIENVLFYIGRVNK
Sequence of entity 4 (D, O), FASTA
>8ZJC_4 Cytochrome c1, heme protein, mitochondrial (chains D, O)
MTAAEHGLHAPAYAWSHNGPFETFDHASIRRGYQVYREVCAACHSLDRVAWRTLVGVSHT
NEEVRNMAEEFEYDDEPDEQGNPKKRPGKLSDYIPGPYPNEQAARAANQGALPPDLSLIV
KARHGGCDYIFSLLTGYPDEPPAGVALPPGSNYNPYFPGGSIAMARVLFDDMVEYEDGTP
ATTSQMAKDVTTFLNWCAEPEHDERKRLGLKTVIILSSLYLLSIWVKKFKWAGIKTRKFV
FNPPKPRK
Sequence of entity 5 (E, P), FASTA
>8ZJC_5 Cytochrome b-c1 complex subunit Rieske, mitochondrial (chains E, P)
KSTYRTPNFDDVLKENNDADKGRSYAYFMVGAMGLLSSAGAKSTVETFISSMTATADVLA
MAKVEVNLAAIPLGKNVVVKWQGKPVFIRHRTPHEIQEANSVDMSALKDPQTDADRVKDP
QWLIMLGICTHLGCVPIGEAGDFGGWFCPCHGSHYDISGRIRKGPAPLNLEIPAYEFDGD
KVIVG
Sequence of entity 6 (F, Q), FASTA
>8ZJC_6 Cytochrome b-c1 complex subunit 6, mitochondrial (chains F, Q)
EVTDQLEDLREHFKNTEEGKALVHHYEECAERVKIQQQQPGYADLEHKEDCVEEFFHLQH
YLDTATAPRLFDKLK
Sequence of entity 7 (G, R), FASTA
>8ZJC_7 Cytochrome b-c1 complex subunit 7 (chains G, R)
PQSFTSIARIGDYILKSPVLSKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALR
RLPEDESYARAYRIIRAHQTELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDELD
NIEVSK
Sequence of entity 8 (H, S), FASTA
>8ZJC_8 Cytochrome b-c1 complex subunit 8 (chains H, S)
GPPSGKTYMGWWGHMGGPKQKGITSYAVSPYAQKPLQGIFHNAVFNSFRRFKSQFLYVLI
PAGIYWYWWKNGNEYNEFLYSKAGREELERVNV
Sequence of entity 9 (I, T), FASTA
>8ZJC_9 Cytochrome b-c1 complex subunit 9, mitochondrial (chains I, T)
SSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHNKGKLWKDVKARIAA
Sequence of entity 10 (U, V), FASTA
>8ZJC_10 Cytochrome b-c1 complex subunit 10, mitochondrial (chains U, V)
KTGLHFGRLSLRSLTAYAPNLMLWGGASMLGLFVFTEGWPKFQDTLYKKIPL
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| UQ6 | 5-(3,7,11,15,19,23-hexamethyl-tetracosa-2,6,10,14,18,22-hexaenyl)-2,3-dimethoxy… | C39 H60 O4 | 2 |
| 8PE | (2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl… | C37 H74 N O8 P | 2 |
| 9PE | (1R)-2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(heptanoyloxy)methyl]et… | C30 H60 N O8 P | 2 |
| HEM | Protoporphyrin IX containing FE | C34 H32 Fe N4 O4 | 6 |
| CN5 | (5S,11R)-5,8,11-trihydroxy-5,11-dioxido-17-oxo-4,6,10,12,16-pentaoxa-5,11-dipho… | C26 H52 O13 P2 | 1 |
| CN3 | (2R,5S,11R,14R)-5,8,11-trihydroxy-2-(nonanoyloxy)-5,11-dioxido-16-oxo-14-[(prop… | C36 H68 O17 P2 | 2 |
| FES | FE2/S2 (inorganic) cluster | Fe2 S2 | 2 |
| 6PH | (1R)-2-(phosphonooxy)-1-[(tridecanoyloxy)methyl]ethyl pentadecanoate | C31 H61 O8 P | 2 |
Primary citation
Cryo-EM structure of Saccharomyces cerevisiae bc1 complex. Ye, Y., Li, Z.W., Yang, G.F. To be published.
Other PDB entries of the same protein (UniProt P07256 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 3CX5 1.9 Å, Structure of complex III with bound cytochrome c in reduced state and definition of a…
- 1EZV 2.3 Å, Structure of the yeast cytochrome BC1 complex co-crystallized with an antibody fv-fragment
- 1KB9 2.3 Å, Yeast cytochrome BC1 complex
- 2IBZ 2.3 Å, Yeast Cytochrome BC1 Complex with Stigmatellin
- 8YIO 2.35 Å, Cryo-EM structure of Saccharomyces cerevisiae bc1 complex in azoxystrobin-bound state
- 9ETZ 2.4 Å, III2IV respiratory supercomplex from Saccharomyces cerevisiae
- 8YHQ 2.42 Å, Cryo-EM structure of Saccharomyces cerevisiae bc1 complex in pyraclostrobin-bound state
- 1P84 2.5 Å, HDBT inhibited Yeast Cytochrome bc1 Complex
- 3CXH 2.5 Å, Structure of yeast complex III with isoform-2 cytochrome c bound and definition of a…
- 8ZMT 2.52 Å, Cryo-EM structure of Saccharomyces cerevisiae bc1 complex in Metyltetraprole-bound state
- 9BPB 2.57 Å, Tethered respiratory III2IV2 supercomplex from Saccharomyces cerevisiae
- 8YIL 2.58 Å, Cryo-EM structure of Saccharomyces cerevisiae bc1 complex in YF24228-bound state
Browse structure collections
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