Cryo-EM structure of human testis-specific Na+,K+-ATPase alpha4 in ouabain-bound form. Determined by electron microscopy at 2.37 Å resolution. Released 4 Dec 2024.
Explore 8ZYJ in 3D Show helices and sheets RCSB PDB PDBe
8ZYJ contains 71 α-helices and 54 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 56-63 | 8 | |
| α-helix | 73-83 | 11 | |
| α-helix | 88-95 | 8 | |
| α-helix | 96-104 | 9 | |
| α-helix | 107-129 | 23 | |
| α-helix | 136-169 | 34 | |
| β-strand | 176 | 1 | 1 |
| β-strand | 179-181 | 3 | 2 |
| β-strand | 184-186 | 3 | 2 |
| β-strand | 189 | 1 | 1 |
| α-helix | 190-192 | 3 | |
| β-strand | 198-202 | 5 | 2 |
| β-strand | 206 | 1 | 2 |
| β-strand | 210-222 | 13 | 2 |
| α-helix | 224-227 | 4 | |
| β-strand | 233-235 | 3 | 2 |
| β-strand | 250-251 | 2 | 2 |
| β-strand | 256-268 | 13 | 2 |
| α-helix | 270-272 | 3 | |
| α-helix | 274-283 | 10 | |
| α-helix | 291-320 | 30 | |
| α-helix | 325-338 | 14 | |
| α-helix | 342-360 | 19 | |
| β-strand | 364-366 | 3 | 3 |
| α-helix | 371-377 | 7 | |
| β-strand | 380-383 | 4 | 3 |
| α-helix | 385-389 | 5 | |
| β-strand | 390 | 1 | 4 |
| β-strand | 395-401 | 7 | 5 |
| β-strand | 404-407 | 4 | 5 |
| α-helix | 424-435 | 12 | |
| β-strand | 441 | 1 | 6 |
| α-helix | 450-452 | 3 | |
| β-strand | 455 | 1 | 6 |
| α-helix | 459-471 | 13 | |
| α-helix | 475-481 | 7 | |
| β-strand | 484-486 | 3 | 5 |
| β-strand | 491 | 1 | 5 |
| β-strand | 496-502 | 7 | 5 |
| β-strand | 510-516 | 7 | 5 |
| α-helix | 518-521 | 4 | |
| β-strand | 525-528 | 4 | 5 |
| β-strand | 529 | 1 | 7 |
| β-strand | 534 | 1 | 7 |
| α-helix | 540-542 | 3 | |
| α-helix | 544-552 | 9 | |
| β-strand | 558-564 | 7 | 5 |
| α-helix | 569-571 | 3 | |
| β-strand | 589-598 | 10 | 5 |
| α-helix | 599-600 | 2 | |
| β-strand | 601 | 1 | 4 |
| α-helix | 602 | 1 | |
| α-helix | 605-614 | 10 | |
| β-strand | 618-622 | 5 | 3 |
| α-helix | 627-637 | 11 | |
| β-strand | 646 | 1 | 3 |
| α-helix | 647-654 | 8 | |
| β-strand | 668-672 | 5 | 3 |
| α-helix | 673-676 | 4 | |
| α-helix | 681-690 | 10 | |
| β-strand | 693-697 | 5 | 3 |
| α-helix | 701-713 | 13 | |
| β-strand | 718-722 | 5 | 3 |
| α-helix | 725-727 | 3 | |
| α-helix | 728-733 | 6 | |
| β-strand | 736-740 | 5 | 3 |
| α-helix | 746-751 | 6 | |
| β-strand | 754-756 | 3 | 3 |
| α-helix | 762-787 | 26 | |
| α-helix | 790-802 | 13 | |
| α-helix | 804-806 | 3 | |
| α-helix | 810-815 | 6 | |
| α-helix | 816-820 | 5 | |
| α-helix | 822-827 | 6 | |
| α-helix | 828-830 | 3 | |
| α-helix | 831-833 | 3 | |
| α-helix | 837-839 | 3 | |
| α-helix | 841-843 | 3 | |
| α-helix | 853-856 | 4 | |
| α-helix | 857-863 | 7 | |
| α-helix | 864-881 | 18 | |
| α-helix | 886-889 | 4 | |
| α-helix | 893-896 | 4 | |
| β-strand | 904-905 | 2 | 8 |
| β-strand | 911-912 | 2 | 8 |
| α-helix | 914-942 | 29 | |
| α-helix | 950-953 | 4 | |
| α-helix | 958-976 | 19 | |
| α-helix | 980-983 | 4 | |
| α-helix | 991-994 | 4 | |
| α-helix | 995-997 | 3 | |
| α-helix | 998-1017 | 20 | |
| α-helix | 1022-1027 | 6 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 24 | 1 | 9 |
| β-strand | 27 | 1 | 9 |
| α-helix | 29-59 | 31 | |
| α-helix | 73 | 1 | |
| α-helix | 75 | 1 | |
| β-strand | 76 | 1 | 10 |
| β-strand | 77-80 | 4 | 11 |
| β-strand | 87-90 | 4 | 12 |
| α-helix | 99-108 | 10 | |
| α-helix | 109-112 | 4 | |
| α-helix | 114-116 | 3 | |
| β-strand | 123-124 | 2 | 13 |
| α-helix | 132-134 | 3 | |
| α-helix | 146-147 | 2 | |
| β-strand | 148-149 | 2 | 13 |
| α-helix | 153-159 | 7 | |
| β-strand | 175-180 | 6 | 11 |
| β-strand | 184 | 1 | 14 |
| α-helix | 190-191 | 2 | |
| β-strand | 208-210 | 3 | 15 |
| β-strand | 211-215 | 5 | 12 |
| α-helix | 218-223 | 6 | |
| β-strand | 227-230 | 4 | 11 |
| β-strand | 237-239 | 3 | 15 |
| α-helix | 240-242 | 3 | |
| β-strand | 245 | 1 | 14 |
| α-helix | 247-250 | 4 | |
| β-strand | 259-263 | 5 | 11 |
| α-helix | 266-267 | 2 | |
| β-strand | 272-278 | 7 | 12 |
| β-strand | 292 | 1 | 10 |
| β-strand | 294-301 | 8 | 12 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Sodium/potassium-transporting ATPase subunit alpha-4 | A | protein | 984 | Homo sapiens | Q13733 (AlphaFold model) |
| Sodium/potassium-transporting ATPase subunit beta-1 | B | protein | 303 | Homo sapiens | P05026 (AlphaFold model) |
>8ZYJ_1 Sodium/potassium-transporting ATPase subunit alpha-4 (chains A) MVVMDDHKLTLEELSTKYSVDLTKGHSHQRAKEILTRGGPNTVTPPPTTPEWVKFCKQLF GGFSLLLWTGAILCFVAYSIQIYFNEEPTKDNLYLSIVLSVVVIVTGCFSYYQEAKSSKI MESFKNMVPQQALVIRGGEKMQINVQEVVLGDLVEIKGGDRVPADLRLISAQGCKVDNSS LTGESEPQSRSPDFTHENPLETRNICFFSTNCVEGTARGIVIATGDSTVMGRIASLTSGL AVGQTPIAAEIEHFIHLITVVAVFLGVTFFALSLLLGYGWLEAIIFLIGIIVANVPEGLL ATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDMTV YEADTTEEQTGKTFTKSSDTWFMLARIAGLCNRADFKANQEILPIAKRATTGDASESALL KFIEQSYSSVAEMREKNPKVAEIPFNSTNKYQMSIHLREDSSQTHVLMMKGAPERILEFC STFLLNGQEYSMNDEMKEAFQNAYLELGGLGERVLGFCFLNLPSSFSKGFPFNTDEINFP MDNLCFVGLISMIDPPRAAVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGTE TAEEVAARLKIPISKVDASAAKAIVVHGAELKDIQSKQLDQILQNHPEIVFARTSPQQKL IIVEGCQRLGAVVAVTGDGVNDSPALKKADIGIAMGISGSDVSKQAADMILLDDNFASIV TGVEEGRLIFDNLKKSIMYTLTSNIPEITPFLMFIILGIPLPLGTITILCIDLGTDMVPA ISLAYESAESDIMKRLPRNPKTDNLVNHRLIGMAYGQIGMIQALAGFFTYFVILAENGFR PVDLLGIRLHWEDKYLNDLEDSYGQQWTYEQRKVVEFTCQTAFFVTIVVVQWADLIISKT RRNSLFQQGMRNKVLIFGILEETLLAAFLSYTPGMDVALRMYPLKITWWLCAIPYSILIF VYDEIRKLLIRQHPDGWVERETYY
>8ZYJ_2 Sodium/potassium-transporting ATPase subunit beta-1 (chains B) MARGKAKEEGSWKKFIWNSEKKEFLGRTGGSWFKILLFYVIFYGCLAGIFIGTIQVMLLT ISEFKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIVRFLEKYKDSAQRDD MIFEDCGDVPSEPKERGDFNHERGERKVCRFKLEWLGNCSGLNDETYGYKEGKPCIIIKL NRVLGFKPKPPKNESLETYPVMKYNPNVLPVQCTGKRDEDKDKVGNVEYFGLGNSPGFPL QYYPYYGKLLQPKYLQPLLAVQFTNLTMDTEIRIECKAYGENIGYSEKDRFQGRFDVKIE VKS
| ID | Name | Formula | Copies |
|---|---|---|---|
| BEF | Beryllium trifluoride ion | Be F3 | 1 |
| PCW | 1,2-dioleoyl-sn-glycero-3-phosphocholine | C44 H85 N O8 P | 1 |
| Q7G | 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9… | C56 H92 O25 | 3 |
| OBN | Ouabain | C29 H44 O12 | 1 |
| CLR | Cholesterol | C27 H46 O | 1 |
| MG | Magnesium ion | Mg | 1 |
| NAG | 2-acetamido-2-deoxy-beta-D-glucopyranose | C8 H15 N O6 | 2 |
Water and common crystallization additives (NA) are not listed.
Molecular Structure of the Na + ,K + -ATPase alpha 4 beta 1 Isoform in Its Ouabain-Bound Conformation. Abe, K., McDermott, J., Valia Madapally, H. et al. Int J Mol Sci (2024) 25. DOI 10.3390/ijms252212397 · PubMed
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