9EK8: Light-harvesting protein B-800/850 alpha chain

Light Harvesting complex 2 (LH2), B800-B850, of Rhodoblastus (Rbl.) acidophilus strain 7750. Determined by electron microscopy at 2.35 Å resolution. Released 30 Jul 2025.

Method: Electron microscopy
Resolution: 2.35 Å
Organism: Rhodoblastus acidophilus
Chains: 18
Atoms: 8,847
Mol. weight: 124.13 kDa
Ligands: LDA, IRM, LMT, BCL
Released: 30 Jul 2025

Explore 9EK8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9EK8 contains 41 α-helices and 0 β-strands across 18 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, C, E, G, I, K, M, O and Q: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	6-8	3
α-helix	12-37	26
α-helix	40-46	7

Chains B, J, L, N and R: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	2-4	3
α-helix	5-36	32

Chains D, F, H and P: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	5-36	32

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Light-harvesting protein B-800/850 alpha chain	A, C, E, G, I, K, M, O, Q	protein	48	Rhodoblastus acidophilus	P26789 (AlphaFold model)
Light-harvesting protein B-800/850 beta chain	B, D, F, H, J, L, N, P, R	protein	41	Rhodoblastus acidophilus	P26790 (AlphaFold model)

Sequence of entity 1 (A, C, E, G, I, K, M, O, Q), FASTA

>9EK8_1 Light-harvesting protein B-800/850 alpha chain (chains A, C, E, G, I, K, M, O, Q)
MNQGKIWTVVNPAIGIPALLGSVTVIAILVHLAILSHTTWFPAYWQGG

Sequence of entity 2 (B, D, F, H, J, L, N, P, R), FASTA

>9EK8_2 Light-harvesting protein B-800/850 beta chain (chains B, D, F, H, J, L, N, P, R)
ATLTAEQSEELHKYVIDGTRVFLGLALVAHFLAFSATPWLH

Ligands and cofactors

ID	Name	Formula	Copies
LDA	Lauryl dimethylamine-N-oxide	C14 H31 N O	9
IRM	1,2-Dihydro-psi,psi-caroten-1-ol	C40 H58 O	9
LMT	Dodecyl-beta-D-maltoside	C24 H46 O11	9
BCL	Bacteriochlorophyll a	C55 H74 Mg N4 O6	27

Primary citation

Robust light-harvesting properties upon low-light acclimation in purple bacteria. Wang, D., Harris, D., Chuang, C. et al. Chem (2025) 11:102597. DOI 10.1016/j.chempr.2025.102597

Other PDB entries of the same protein (UniProt P26789 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1NKZ 2.0 Å, Crystal structure of LH2 B800-850 from Rps. acidophila at 2.0 Angstrom resolution
2FKW 2.45 Å, Structure of LH2 from Rps. acidophila crystallized in lipidic mesophases
1KZU 2.5 Å, Integral membrane peripheral light harvesting complex from rhodopseudomonas acidophila…

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