9G40: Gamma-tubulin complex component 3

Structure of the Position 7 CMG-decorated gamma-Tubulin Ring Complex from Pig Brain. Determined by electron microscopy at 4.3 Å resolution. Released 2 Oct 2024.

Method
Electron microscopy
Resolution
4.3 Å
Organisms
Sus scrofa, Homo sapiens
Chains
5
Atoms
12,443
Mol. weight
652.39 kDa
Released
2 Oct 2024

Explore 9G40 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9G40 contains 76 α-helices and 16 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain F: 29 helices, 8 β-strands

ElementResiduesLengthSheet
α-helix250-26112
β-strand26611
β-strand26911
β-strand270-27232
β-strand277-27932
α-helix282-2843
α-helix288-31528
α-helix322-34928
α-helix367-38822
α-helix397-4048
α-helix412-43827
α-helix462-4654
α-helix471-4733
α-helix480-49819
α-helix533-55119
α-helix552-5565
α-helix558-5647
α-helix565-5695
α-helix574-58815
β-strand59113
α-helix592-5943
α-helix597-61014
α-helix618-6236
β-strand624-62854
β-strand63613
α-helix637-6404
β-strand641-64554
α-helix649-6546
α-helix657-69337
α-helix701-72525
α-helix726-7338
α-helix734-74310
α-helix747-76418
α-helix772-81847
α-helix826-86843
α-helix875-8828
α-helix894-8963
Chain G: 42 helices, 8 β-strands
ElementResiduesLengthSheet
α-helix5-1713
α-helix26-338
α-helix46-5611
α-helix60-7314
α-helix78-8811
α-helix92-11524
α-helix150-16920
α-helix179-1813
α-helix210-22415
β-strand233-23645
β-strand245-24845
α-helix254-26310
α-helix265-27915
α-helix283-2853
α-helix286-31530
α-helix321-34828
α-helix353-36513
α-helix370-39223
α-helix393-3975
β-strand409-41136
α-helix416-4216
β-strand432-43436
α-helix4351
α-helix441-4466
α-helix447-46216
α-helix473-4753
α-helix482-50221
α-helix503-5075
α-helix509-51810
α-helix525-53915
β-strand54217
α-helix543-5453
α-helix548-56013
β-strand573-57758
α-helix582-5909
α-helix605-6073
β-strand61117
β-strand616-62058
α-helix632-66130
α-helix664-6663
α-helix674-6763
α-helix679-69719
α-helix698-7025
α-helix703-71412
α-helix719-73618
α-helix742-77130
α-helix824-85027
α-helix854-8629
α-helix866-8683
α-helix870-8778
Chain u: 1 helix, 0 β-strands
ElementResiduesLengthSheet
α-helix64-9027
Chain v: 1 helix, 0 β-strands
ElementResiduesLengthSheet
α-helix60-9031
Chain X: 3 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix35-4511
α-helix57-626
α-helix67-7913

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Gamma-tubulin complex component 3Fprotein910Sus scrofaF1RN46 (AlphaFold model)
Gamma-tubulin complex componentGprotein905Sus scrofaA0A480VJI0 (AlphaFold model)
Mitotic-spindle organizing protein 2A isoform X4Xprotein155Sus scrofaF1RK97 (AlphaFold model)
CDK5 regulatory subunit-associated protein 2u, vprotein1893Homo sapiensQ96SN8 (AlphaFold model)
Sequence of entity 1 (F), FASTA
>9G40_1 Gamma-tubulin complex component 3 (chains F)
MATPDQKSPNVLLQNLCCRILGKSEADVAQQFQYAVRVIGSNFAPTVERDEFLVAEKIKK
ELTRQRREADAALFSELHRKLHSQGVLKNKWSILYLLLSLSEDPRKQPSKVSGYAALFAQ
ALPRDAHSTPYYYARPQSLPLNYQERGAPSAQSAGSAGSSGVSSLGTYALNGPTPPPPPP
ALLPGQPLPAPGVGDGLRQQLGSRLAWTLTASQPSLPSTTSKAVPSSGSRGAARPRREGD
AAAGAVEVTEAALVRDILYVFQGIDGKHVKMSNADNCYTVEGKANLSKSLRDTAVRLAEL
GWLHNKIRKYTDQRSLDRSFGLVGQSFCAALHQELREYYRLLSVLHSQLQLEDDQGVNLG
LESSLTLRRLLVWTYDPKMRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYARSLVQH
ILSLVSHPVLSFLYRWIYDGELEDTYHEFFVASDPAVKADRLWHDKYALRKPMIPSFMTM
DQCRKVLLIGKSINFLHQVCHDQTPTTKMIAVTKSAESPQDAADLFTDLENAFQGKIDAA
YFETSKYLLDVLNKKYSLLDHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQHNL
TGILETAVRATNAQFDSPEILKRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTRECM
SHYLRAFNFLWRAKRVEYILTDIRKGHMCNARLLRSMPEFSGVLHHCHILASEMVHFIHQ
MQYYVTFEVLECSWDELWNRVQRAQDLDHIIAAHEAFLGTVISRCLLDSDSRALLNQLRA
VFDQIIELQNTQDAIYRAALEELQRRLQFEEKKKQREAEGQWGVSAAEEEQEKRRVQEFQ
ESIPKMCSQLRILTHFYQGVVQQFLVSLTTSSDESLRFLSFRLDFNEHYRAREPRLRVSL
GTRGRRSSHT
Sequence of entity 2 (G), FASTA
>9G40_2 Gamma-tubulin complex component (chains G)
MSEFRIHHDVNELLSLLRVHGGDGAEVYIDLLQKNRTPYVTTTVSAHSAKVKIAEFSRTP
EDFLKKYDELKSKNTRNLDPLVYLLSKLMEDRETLQYLQQNAKERAELAASAAASSTASF
GASATASKISMQELEELRKQLGSVATGPTWQQSLELTRKMLRDKQSKKNSGQRLPVLPAW
VYERPALLGDFLPGTGGSADTAVPIGSLPLASQEAAVVEDLLYVLVGVDGRYISAQPLTG
RQGRTFLVDPNLDLSIRELVSRILPVAASYSTVTRFIEEKSSFEYGQVNHALAAAMRTLV
KEYLVLVTQLEQLQRQGLLSLQKLWFYIQPAMRSLDILASLATSVDKGECIGGATLSLLH
DRSFSYTGDSQAQELCLHLTKAASTPYFEILEKWIYRGIIDDPYSEFMVEEHELRKEKIQ
EDYNDKYWDQRYTVVQRQIPSFLQKMAGKVLSTGKYLNVVRECGHDVTCPVAKEVVYTLK
ERAYVEQIEKAFSYASKVLLDFLMGEKELLAHLRSIKRYFLMDQGDFFVHFMDLTEEELK
KPVDDITPTRLEALLELALRMSTANTDPFKDDLKIDLMPHDLITQLLRVLAIETQQEKAM
VHADPTELTLSGLEAFSFDYVVTWPLSLIINRKALTRYQMLFRHMFYCKHVERQLCSVWI
SNKAAKRFSLHSAKWFAGAFTLRQRMLNFVQNIQSYMMFEVMEPTWHVLEQNLRSASNID
DVLGHHASFLDNCLKDCMLTNPELLRVFSKLMSVCVMFTNCLQRFTQSMKLDSELGHPAL
EPGAMLGPPTEAERAEERARKELARKCLAEHVDAPQLASSFEATITKFDKNFSAHLLDLL
ARLSIYSTSDCEHGMASVISRLDFNGFYTERLERLSAERSQKAAPPVPGPRGPPALVPRV
AVTAQ
Sequence of entity 3 (X), FASTA
>9G40_3 Mitotic-spindle organizing protein 2A isoform X4 (chains X)
MAAPGAGPGPGAPPGLEAALQKLALRRKKVLSAEETELFELAQAAGGAMDPEVFKILVDL
LKLNVAPLAVFQMLKSMCAGQRLASEPQDPVAVPLPTTSVPETRGRNRGSSALGGGPALA
ERSGREGSSQRMPRQPSATRLPKGGGPGKSPTRST
Sequence of entity 4 (u, v), FASTA
>9G40_4 CDK5 regulatory subunit-associated protein 2 (chains u, v)
MMDLVLEEDVTVPGTLSGCSGLVPSVPDDLDGINPNAGLGNGLLPNVSEETVSPTRARNM
KDFENQITELKKENFNLKLRIYFLEERMQQEFHGPTEHIYKTNIELKVEVESLKRELQER
EQLLIKASKAVESLAEAGGSEIQRVKEDARKKVQQVEDLLTKRILLLEKDVTAAQAELEK
AFAGTETEKALRLRLESKLSEMKKMHEGDLAMALVLDEKDRLIEELKLSLKSKEALIQCL
KEEKSQMACPDENVSSGELRGLCAAPREEKERETEAAQMEHQKERNSFEERIQALEEDLR
EKEREIATEKKNSLKRDKAIQGLTMALKSKEKKVEELNSEIEKLSAAFAKAREALQKAQT
QEFQGSEDYETALSGKEALSAALRSQNLTKSTENHRLRRSIKKITQELSDLQQERERLEK
DLEEAHREKSKGDCTIRDLRNEVEKLRNEVNEREKAMENRYKSLLSESNKKLHNQEQVIK
HLTESTNQKDVLLQKFNEKDLEVIQQNCYLMAAEDLELRSEGLITEKCSSQQPPGSKTIF
SKEKKQSSDYEELIQVLKKEQDIYTHLVKSLQESDSINNLQAELNKIFALRKQLEQDVLS
YQNLRKTLEEQISEIRRREEESFSLYSDQTSYLSICLEENNRFQVEHFSQEELKKKVSDL
IQLVKELYTDNQHLKKTIFDLSCMGFQGNGFPDRLASTEQTELLASKEDEDTIKIGEDDE
INFLSDQHLQQSNEIMKDLSKGGCKNGYLRHTESKISDCDGAHAPGCLEEGAFINLLAPL
FNEKATLLLESRPDLLKVVRELLLGQLFLTEQEVSGEHLDGKTEKTPKQKGELVHFVQTN
SFSKPHDELKLSCEAQLVKAGEVPKVGLKDASVQTVATEGDLLRFKHEATREAWEEKPIN
TALSAEHRPENLHGVPGWQAALLSLPGITNREAKKSRLPILIKPSRSLGNMYRLPATQEV
VTQLQSQILELQGELKEFKTCNKQLHQKLILAEAVMEGRPTPDKTLLNAQPPVGAAYQDS
PGEQKGIKTTSSVWRDKEMDSDQQRSYEIDSEICPPDDLASLPSCKENPEDVLSPTSVAT
YLSSKSQPSAKVSVMGTDQSESINTSNETEYLKQKIHDLETELEGYQNFIFQLQKHSQCS
EAIITVLCGTEGAQDGLSKPKNGSDGEEMTFSSLHQVRYVKHVKILGPLAPEMIDSRVLE
NLKQQLEEQEYKLQKEQNLNMQLFSEIHNLQNKFRDLSPPRYDSLVQSQARELSLQRQQI
KDGHGICVISRQHMNTMIKAFEELLQASDVDYCVAEGFQEQLNQCAELLEKLEKLFLNGK
SVGVEMNTQNELMERIEEDNLTYQHLLPESPEPSASHALSDYETSEKSFFSRDQKQDNET
EKTSVMVNSFSQDLLMEHIQEIRTLRKRLEESIKTNEKLRKQLERQGSEFVQGSTSIFAS
GSELHSSLTSEIHFLRKQNQALNAMLIKGSRDKQKENDKLRESLSRKTVSLEHLQREYAS
VKEENERLQKEGSEKERHNQQLIQEVRCSGQELSRVQEEVKLRQQLLSQNDKLLQSLRVE
LKAYEKLDEEHRRLREASGEGWKGQDPFRDLHSLLMEIQALRLQLERSIETSSTLQSRLK
EQLARGAEKAQEGALTLAVQAVSIPEVPLQPDKHDGDKYPMESDNSFDLFDSSQAVTPKS
VSETPPLSGNDTDSLSCDSGSSATSTPCVSRLVTGHHLWASKNGRHVLGLIEDYEALLKQ
ISQGQRLLAEMDIQTQEAPSSTSQELGTKGPHPAPLSKFVSSVSTAKLTLEEAYRRLKLL
WRVSLPEDGQCPLHCEQIGEMKAEVTKLHKKLFEQEKKLQNTMKLLQLSKRQEKVIFDQL
VVTHKILRKARGNLELRPGGAHPGTCSPSRPGS

Primary citation

Partial closure of the gamma-tubulin ring complex by CDK5RAP2 activates microtubule nucleation. Xu, Y., Munoz-Hernandez, H., Krutyholowa, R. et al. Dev Cell (2024) 59:3161. DOI 10.1016/j.devcel.2024.09.002 · PubMed

Other PDB entries of the same protein (UniProt F1RN46 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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