9JR2: PTH-PTH1R-Gq

Cryo-EM structure of PTH-PTH1R-Gq (upright state). Determined by electron microscopy at 2.8 Å resolution. Released 16 Oct 2024.

Method: Electron microscopy
Resolution: 2.8 Å
Organisms: Homo sapiens, Rattus rattus, Bos taurus
Chains: 6
Atoms: 9,418
Mol. weight: 168.86 kDa
Released: 16 Oct 2024

Explore 9JR2 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9JR2 contains 41 α-helices and 70 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	34-39	6	1
α-helix	46-48	3
β-strand	69	1	2
β-strand	72-76	5	1
β-strand	79-85	7	1
β-strand	86	1	2
α-helix	97-100	4
β-strand	105-111	7	1
α-helix	118-129	12
α-helix	132-134	3
α-helix	137-138	2
β-strand	139-144	6	1
α-helix	146-155	10
α-helix	160-163	4
α-helix	165-169	5
α-helix	184-201	18
β-strand	211-215	5	1
α-helix	224-243	20

Chain B: 4 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	5-24	20
α-helix	30-33	4
β-strand	43-44	2	3
β-strand	47-51	5	4
β-strand	58-63	6	5
β-strand	69-74	6	5
β-strand	78-83	6	5
β-strand	88-94	7	5
β-strand	100-105	6	6
β-strand	111-116	6	6
β-strand	120-125	6	6
α-helix	129-131	3
β-strand	134-140	7	6
β-strand	146-151	6	7
β-strand	156-161	6	7
β-strand	165-170	6	7
β-strand	175-181	7	7
β-strand	187-192	6	8
β-strand	198-203	6	8
β-strand	208-212	5	8
β-strand	218-222	5	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	260-264	5	9
α-helix	272	1
β-strand	273-278	6	3
β-strand	284-289	6	3
β-strand	294-298	5	3
β-strand	304-308	5	3
β-strand	315-320	6	4
β-strand	327-331	5	4
β-strand	336-339	4	4

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	12-23	12
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chain P: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	3-33	31

Chain R: 18 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	34-53	20
β-strand	111-112	2	16
β-strand	117-118	2	16
β-strand	127-130	4	17
α-helix	131-132	2
β-strand	143-146	4	17
α-helix	155	1
β-strand	156	1	18
α-helix	157	1
β-strand	163	1	18
β-strand	165-166	2	17
α-helix	169-171	3
α-helix	179-211	33
α-helix	213-215	3
α-helix	218-244	27
α-helix	279-311	33
α-helix	318-346	29
α-helix	361-390	30
α-helix	400-412	13
α-helix	414-417	4
α-helix	419-422	4
α-helix	435-447	13
α-helix	451-455	5
α-helix	456-461	6
α-helix	464-481	18

Chain S: 3 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	10-12	3	11
β-strand	18-25	8	10
α-helix	29-31	3
β-strand	33-39	7	11
β-strand	45-51	7	11
α-helix	53-55	3
β-strand	58-60	3	11
β-strand	69-73	5	10
β-strand	78-83	6	10
α-helix	88-90	3
β-strand	92-99	8	11
β-strand	110	1	11
β-strand	113	1	10
β-strand	115-119	5	11
β-strand	128-129	2	12
β-strand	134-136	3	13
β-strand	143-146	4	14
β-strand	148-149	2	12
β-strand	154	1	15
β-strand	160	1	15
β-strand	162-167	6	13
β-strand	174-178	5	13
β-strand	182-183	2	13
β-strand	191-196	6	14
β-strand	199-204	6	14
β-strand	214-219	6	13
β-strand	227	1	13
β-strand	231-234	4	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(q) subunit alpha-1 (miniGq)	A	protein	245	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	344	Rattus rattus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	67	Bos taurus	P63212 (AlphaFold model)
scFv16	S	protein	260	Mus musculus
Parathyroid hormone	P	protein	35	Homo sapiens	P01270 (AlphaFold model)
Parathyroid hormone/parathyroid hormone-related peptide receptor	R	protein	567	Homo sapiens	Q03431

Sequence of entity 1 (A), FASTA

>9JR2_1 Guanine nucleotide-binding protein G(q) subunit alpha-1 (miniGq) (chains A)
GSTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMRILHGGSG
GSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEA
LNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPG
EDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNLR
EYNLV

Sequence of entity 2 (B), FASTA

>9JR2_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
GSQLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLA
KIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGG
LDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQ
TTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFP
NGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNV
WDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>9JR2_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
ASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF
REKKFFS

Sequence of entity 4 (S), FASTA

>9JR2_4 scFv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAASSEDLYFQ

Sequence of entity 5 (P), FASTA

>9JR2_5 Parathyroid hormone (chains P)
SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFX

Sequence of entity 6 (R), FASTA

>9JR2_6 Parathyroid hormone/parathyroid hormone-related peptide receptor (chains R)
DADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGK
LYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYR
RCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLGMIYTVGYSVSLASLTVAV
LILAYFRRLHCTRNYIHMHLFLSFMLRAVSIFVKDAVLYSGATLDEAERLTEEELRAIAQ
APPPPATAAAGYAGCRVAVTFFLYFLATNYYWILVEGLYLHSLIFMAFFSEKKYLWGFTV
FGWGLPAVFVAVWVSVRATLANTGCWDLSSGNKKWIIQVPILASIVLNFILFINIVRVLA
TKLRETNAGRCDTRQQYRKLLKSTLVLMPLFGVHYIVFMATPYTEVSGTLWQVQMHYEML
FNSFQGFFVAIIYCFCNGEVQAEIKKSWSRWTLALDFKRKARSGSSSYSYGPMVSHTSVT
NVGPRVGLGLPLSPRLLPTATTNGHPQLPGHAKPGTPALETLETTPPAMAAPKDDGFLNG
SCSGLDEEASGPERPPALLQEEWETVM

Primary citation

Insights into G-protein coupling preference from cryo-EM structures of G q -bound PTH1R. Sano, F.K., Shimizume, K., Kobayashi, K. et al. Nat Chem Biol (2025) 21:1906-1914. DOI 10.1038/s41589-025-01957-6 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

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