9JUS: Villin

Structure of villin bound to an actin trimer. Determined by X-ray diffraction at 2.7 Å resolution. Released 8 Oct 2025.

Method: X-ray diffraction
Resolution: 2.7 Å
Organisms: Gallus gallus, Paralvinella sulfincola
Chains: 8
Atoms: 30,576
Mol. weight: 442.88 kDa
Ligands: ADP, MG, CA, SCN
Released: 8 Oct 2025

Explore 9JUS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9JUS contains 224 α-helices and 198 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain f: 25 helices, 21 β-strands

Element	Residues	Length	Sheet
α-helix	6-7	2
β-strand	8-12	5	6
β-strand	16-21	6	6
β-strand	24	1	7
β-strand	29-32	4	6
β-strand	35-38	4	8
β-strand	42	1	9
β-strand	53-54	2	8
α-helix	56-60	5
α-helix	62-64	3
β-strand	65-68	4	8
β-strand	71-72	2	10
β-strand	75-76	2	10
α-helix	79-88	10
α-helix	89-93	5
α-helix	98-100	3
β-strand	103-107	5	6
α-helix	113-121	9
α-helix	122-126	5
β-strand	131-136	6	6
α-helix	137-144	8
β-strand	150-155	6	11
β-strand	160-166	7	11
β-strand	169-170	2	11
α-helix	172-174	3
β-strand	176-178	3	11
α-helix	182-192	11
α-helix	203-216	14
α-helix	223-232	10
β-strand	238-241	4	12
β-strand	247-250	4	12
α-helix	253-259	7
α-helix	264-267	4
α-helix	272-273	2
α-helix	274-282	9
α-helix	287-294	8
β-strand	297-300	4	11
α-helix	302-304	3
α-helix	309-320	12
β-strand	329-330	2	11
α-helix	335-337	3
α-helix	338-346	9
α-helix	350-355	6
β-strand	357-358	2	6
α-helix	359-365	7
α-helix	367-370	4

Chain F: 26 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	8-12	5	31
β-strand	16-21	6	31
β-strand	24	1	32
β-strand	29-32	4	31
β-strand	35-38	4	33
β-strand	42	1	34
β-strand	53-54	2	33
α-helix	56-60	5
α-helix	62-64	3
β-strand	65-68	4	33
β-strand	71-72	2	35
β-strand	75-76	2	35
α-helix	79-87	9
α-helix	88-93	6
α-helix	98-100	3
β-strand	103-107	5	31
α-helix	113-121	9
α-helix	122-126	5
β-strand	132-136	5	31
α-helix	137-144	8
β-strand	150-155	6	36
β-strand	160-166	7	36
β-strand	169-170	2	36
α-helix	172-174	3
β-strand	176-178	3	36
α-helix	182-193	12
α-helix	194-196	3
α-helix	203-216	14
α-helix	223-232	10
α-helix	234-236	3
β-strand	238-241	4	37
β-strand	247-250	4	37
α-helix	252-256	5
α-helix	259-262	4
α-helix	264-267	4
α-helix	274-282	9
α-helix	287-294	8
β-strand	297-300	4	36
α-helix	302-304	3
α-helix	309-320	12
β-strand	329-330	2	36
α-helix	335-337	3
α-helix	338-346	9
α-helix	350-355	6
α-helix	359-365	7
α-helix	370-373	4

Chain g: 25 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	5-7	3
β-strand	8-12	5	13
β-strand	16-21	6	13
β-strand	29-32	4	13
β-strand	35-37	3	14
β-strand	53-54	2	14
α-helix	56-59	4
β-strand	66-68	3	14
α-helix	79-87	9
α-helix	88-94	7
α-helix	98-100	3
β-strand	103-107	5	13
α-helix	113-121	9
α-helix	122-126	5
β-strand	131-136	6	13
α-helix	137-144	8
β-strand	150-155	6	9
β-strand	160-166	7	9
β-strand	169-170	2	9
α-helix	172-174	3
β-strand	176-178	3	9
α-helix	182-194	13
α-helix	203-216	14
α-helix	223-232	10
β-strand	238-241	4	15
β-strand	247-250	4	15
α-helix	253-259	7
α-helix	264-267	4
α-helix	272-273	2
α-helix	274-283	10
α-helix	287-289	3
α-helix	290-294	5
β-strand	297-300	4	9
α-helix	302-305	4
α-helix	309-320	12
β-strand	329-330	2	9
α-helix	335-337	3
α-helix	338-348	11
α-helix	350-352	3
β-strand	357-358	2	13
α-helix	359-365	7
α-helix	367-371	5

Chain G: 23 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	8-11	4	38
β-strand	16-21	6	38
β-strand	29-32	4	38
β-strand	35-37	3	39
β-strand	53-54	2	39
α-helix	56-60	5
α-helix	62-64	3
β-strand	66-68	3	39
β-strand	71	1	40
β-strand	76	1	40
α-helix	79-87	9
α-helix	88-93	6
α-helix	98-100	3
β-strand	103-107	5	38
α-helix	113-125	13
β-strand	131-136	6	38
α-helix	137-144	8
β-strand	150-155	6	34
β-strand	160-166	7	34
β-strand	169-170	2	34
α-helix	172-174	3
β-strand	176-178	3	34
α-helix	182-194	13
α-helix	203-216	14
α-helix	223-231	9
β-strand	238-241	4	41
β-strand	247-250	4	41
α-helix	253-259	7
α-helix	264-267	4
α-helix	272-273	2
α-helix	274-283	10
α-helix	290-294	5
β-strand	297-300	4	34
α-helix	302-304	3
α-helix	309-320	12
β-strand	329-330	2	34
α-helix	335-337	3
α-helix	338-348	11
α-helix	350-352	3
β-strand	357-358	2	38
α-helix	359-365	7
α-helix	369-373	5

Chain p: 23 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	5-7	3
β-strand	8-12	5	1
β-strand	16-21	6	1
β-strand	29-32	4	1
β-strand	35-36	2	2
β-strand	38	1	3
β-strand	53-54	2	2
α-helix	56-60	5
β-strand	65	1	3
β-strand	68	1	2
α-helix	79-87	9
α-helix	88-93	6
α-helix	98-100	3
β-strand	103-107	5	1
α-helix	113-121	9
α-helix	122-126	5
β-strand	131-136	6	1
α-helix	137-144	8
β-strand	150-155	6	4
β-strand	160-166	7	4
β-strand	169-170	2	4
α-helix	172-174	3
β-strand	176-178	3	4
α-helix	182-194	13
α-helix	203-216	14
α-helix	223-232	10
α-helix	234-236	3
β-strand	238-241	4	5
β-strand	247-250	4	5
α-helix	253-259	7
α-helix	264-267	4
α-helix	274-283	10
α-helix	287-294	8
β-strand	297-300	4	4
α-helix	302-304	3
α-helix	309-320	12
β-strand	329-330	2	4
α-helix	335-337	3
α-helix	338-346	9
α-helix	350-354	5
β-strand	357-358	2	1
α-helix	359-362	4

Chain P: 21 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	8-12	5	26
β-strand	16-21	6	26
β-strand	29-32	4	26
β-strand	35-38	4	27
β-strand	53-54	2	27
α-helix	56-60	5
β-strand	65-68	4	27
α-helix	79-87	9
α-helix	88-93	6
α-helix	98-100	3
β-strand	103-107	5	26
α-helix	113-125	13
β-strand	131-136	6	26
α-helix	137-144	8
β-strand	150-155	6	28
β-strand	160-166	7	28
β-strand	169-170	2	28
α-helix	172-174	3
β-strand	176-178	3	28
α-helix	182-194	13
α-helix	203-216	14
α-helix	223-232	10
β-strand	238-241	4	29
β-strand	247-250	4	29
α-helix	253-259	7
α-helix	264-267	4
α-helix	274-283	10
α-helix	287-289	3
α-helix	290-294	5
β-strand	297-298	2	30
β-strand	299-300	2	28
α-helix	302-305	4
α-helix	309-320	12
β-strand	329-330	2	30
α-helix	335-337	3
α-helix	338-347	10
α-helix	352-355	4
β-strand	357-358	2	26
α-helix	360-363	4

Chain v: 42 helices, 42 β-strands

Element	Residues	Length	Sheet
α-helix	4-6	3
β-strand	15-22	8	16
β-strand	25-28	4	16
α-helix	29-30	2
α-helix	31-33	3
β-strand	36-38	3	17
β-strand	42-51	10	16
β-strand	54-63	10	16
α-helix	69-85	17
β-strand	91-96	6	16
α-helix	102-106	5
β-strand	114-116	3	17
α-helix	120-122	3
β-strand	125	1	7
β-strand	134-139	6	18
β-strand	145-149	5	18
α-helix	154-156	3
β-strand	158	1	19
β-strand	162-166	5	18
β-strand	170-175	6	18
α-helix	181-198	18
β-strand	202-207	6	18
α-helix	213-216	4
α-helix	218-227	10
α-helix	230-232	3
α-helix	233-236	4
β-strand	238	1	19
α-helix	241-243	3
α-helix	246-255	10
β-strand	258-265	8	20
β-strand	268-276	9	20
β-strand	280	1	21
α-helix	281-283	3
β-strand	289-293	5	20
α-helix	295-297	3
β-strand	299-303	5	20
α-helix	309-325	17
β-strand	334-338	5	20
α-helix	344-348	5
β-strand	352	1	21
α-helix	377-379	3
α-helix	381-384	4
β-strand	402-409	8	22
β-strand	412-415	4	22
α-helix	416-417	2
α-helix	418-420	3
β-strand	423-425	3	23
β-strand	429-438	10	22
β-strand	441-450	10	22
α-helix	456-472	17
β-strand	478-483	6	22
α-helix	489-494	6
β-strand	498-502	5	23
β-strand	522-527	6	23
β-strand	534-539	6	23
α-helix	543-545	3
β-strand	547	1	24
β-strand	551-555	5	23
β-strand	560-564	5	23
α-helix	570-581	12
β-strand	587-591	5	23
α-helix	597-601	5
β-strand	610	1	24
α-helix	613-616	4
α-helix	623-625	3
β-strand	626-631	6	25
β-strand	638-641	4	25
α-helix	647-649	3
β-strand	655-660	6	25
β-strand	663-668	6	25
α-helix	674-688	15
β-strand	701-705	5	25
α-helix	711-716	6
α-helix	731-737	7
α-helix	746	1
β-strand	747	1	25
α-helix	748	1
α-helix	764-765	2
α-helix	768-771	4
α-helix	777-778	2
α-helix	783-789	7
α-helix	792-799	8
α-helix	803-808	6
α-helix	811-820	10

Chain V: 39 helices, 42 β-strands

Element	Residues	Length	Sheet
α-helix	4-6	3
β-strand	15-22	8	42
β-strand	25-28	4	42
α-helix	29-30	2
α-helix	31-33	3
β-strand	36-37	2	43
β-strand	42-48	7	42
β-strand	50-51	2	44
β-strand	54-55	2	44
β-strand	58-63	6	42
α-helix	69-85	17
β-strand	91-96	6	42
α-helix	102-105	4
β-strand	114-115	2	43
α-helix	120-122	3
β-strand	125	1	32
β-strand	134-139	6	45
β-strand	145-148	4	45
β-strand	162-166	5	45
β-strand	170-175	6	45
α-helix	181-198	18
β-strand	202-207	6	45
α-helix	213-216	4
α-helix	218-227	10
α-helix	231-234	4
α-helix	246-255	10
β-strand	258-264	7	46
β-strand	269-276	8	46
α-helix	279	1
β-strand	280	1	47
α-helix	281-283	3
β-strand	289-293	5	46
α-helix	295-297	3
β-strand	299-303	5	46
α-helix	309-326	18
β-strand	334-338	5	46
α-helix	344-347	4
β-strand	352	1	47
α-helix	355-358	4
α-helix	381-384	4
α-helix	386-387	2
β-strand	402-409	8	48
β-strand	412-415	4	48
α-helix	416-417	2
α-helix	418-420	3
β-strand	423-425	3	49
β-strand	429-438	10	48
β-strand	441-450	10	48
α-helix	456-472	17
β-strand	477-483	7	48
α-helix	489-494	6
β-strand	498-502	5	49
β-strand	522-528	7	49
β-strand	534-539	6	49
α-helix	543-545	3
β-strand	547	1	50
β-strand	551-555	5	49
β-strand	560-564	5	49
α-helix	570-580	11
β-strand	587-591	5	49
α-helix	597-601	5
β-strand	610	1	50
α-helix	613-616	4
α-helix	623-625	3
β-strand	626-631	6	51
β-strand	638-642	5	51
α-helix	647-649	3
β-strand	655-659	5	51
β-strand	664-668	5	51
α-helix	674-689	16
β-strand	701-705	5	51
α-helix	711-714	4
α-helix	715-717	3
α-helix	731-738	8
β-strand	746-747	2	51
α-helix	768-771	4
α-helix	777-778	2
α-helix	783-789	7
α-helix	792-799	8
α-helix	803-808	6
α-helix	811-820	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Actin, alpha skeletal muscle	F, G, P, f, g, p	protein	377	Gallus gallus	P68139 (AlphaFold model)
villin	V, v	protein	823	Paralvinella sulfincola	A0AAD9N3N6 (AlphaFold model)

Sequence of entity 1 (F, G, P, f, g, p), FASTA

>9JUS_1 Actin, alpha skeletal muscle (chains F, G, P, f, g, p)
MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA
QSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREK
MTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRL
DLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEK
SYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNV
MSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWIT
KQEYDEAGPSIVHRKCF

Sequence of entity 2 (V, v), FASTA

>9JUS_2 villin (chains V, v)
TDPAFRSVPKGTPCFLIWRIENFQPVPVPKDQYGNFFEGDAYIILSQKDNKGILEQNLHF
WLGKNSSQDEQGTAALKTVELDDYLGGTPVQHRECQNNESKLFLSYFKNKSLKYLQGGVA
SGFNHVEHIVRRRLLSVKGKHTPRMEEKPEISWSQMNKGDVFILDLGEIIYVWNGELCSR
TERIKAMEIARGMRDDRGTGNIIVVEDGEETPDDMGEEEFEVFNEYLPVADKEASIKSAE
EGGADENFEKKKVAQLKLWKVAEEDGNLKITEEATAPLDKKMLDSNDCFIVDNGEDGIWV
WTGKKASPKERKESMNNAMAFLKQRNYSSQTRVTKVPEGGESSEFKSLFKTWEKTKLPGS
VKPYSVNKIAQTVQTKFDAMTLHNNPEVAKETGMVDDGSGKKKIYRIENMDLVELEKRYY
GELYGGDSYVIHYTYAVNGKEEHIIYYWLGRHSTSDERGVAAAKTIEIDDSLGGTAKQVR
VVQSKEPNHFMAMFDGKLIIFQGGKAGWGGHNSTDGPGDTYLLHVRGTSQYNTKAEQVPC
RAESLNSNDVFVLFSKGGTYVWAGKGCTGDEREMAKKIASKSPKGYIMIVEGQEKEEFWD
LLGGKTEYASDFSLKQAENEHRPSRLFQCSNASGVFKAEEIVDFVQEDLVPEDVFILDAD
HTIYVWLGNEARNDEKQMAMDTAIEYLESDPSGRDPDTPIMTVKQGYEPPDFVGHFGVWD
RQLWSHGMSYAELKKELGEKNMSMEQVRQRSKQNGEMSFSDVSKYPYSVLVQKEGLPDGI
DLQNKEKHLTEEEFEKIFGMTYATFITKPAWKQTQLKKDKGLF

Ligands and cofactors

ID	Name	Formula	Copies
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	6
MG	Magnesium ion	Mg	6
CA	Calcium ion	Ca	16
SCN	Thiocyanate ion	C N S	1

Water and common crystallization additives (CL) are not listed.

Primary citation

The structure of an actin nucleus stabilized by villin. Robinson, R.C., Chongrungreang, T., Ponlachantra, K. et al. Sci Adv (2025) 11:eadw6915-eadw6915. DOI 10.1126/sciadv.adw6915 · PubMed

Other PDB entries of the same protein (UniProt P68139 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7W4Z 1.15 Å, Crystal structure of fragmin domain-1 in complex with actin (AMPPNP-form)
7W50 1.15 Å, Crystal structure of fragmin domain-1 in complex with actin (ADP-Pi-form)
7W51 1.2 Å, Crystal structure of fragmin domain-1 in complex with actin (ADP-form)
9L2N 1.7 Å, Crystal structure of Cytochalasin D bound to a filamentous conformation actin
7W52 2.0 Å, Crystal structure of fragmin domain-1 (15-160) in complex with actin
1MDU 2.2 Å, Crystal structure of the chicken actin trimer complexed with human gelsolin segment 1…
8D13 2.43 Å, Helical ADP-F-actin
8D14 2.51 Å, Helical ADP-Pi-F-actin
7R94 2.6 Å, T-Plastin-F-actin complex
8C4E 2.6 Å, F-actin decorated by SipA426-685
9JW0 2.69 Å, Structure of the N-terminal 3 domains (V1-V3) villin bound to actin
7YNE 2.7 Å, Crystal structure of fragmin domain-1 (1-160) in complex with G-form actin

Browse structure collections

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