9LWP: Unliganded human BRS3-Gq complex

Cryo-EM structure of the unliganded human BRS3-Gq complex. Determined by electron microscopy at 2.93 Å resolution. Released 16 Jul 2025.

Method: Electron microscopy
Resolution: 2.93 Å
Organisms: Homo sapiens, Oplophorus gracilirostris, Rattus norvegicus
Chains: 4
Atoms: 6,844
Mol. weight: 141.19 kDa
Released: 16 Jul 2025

Explore 9LWP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9LWP contains 27 α-helices and 36 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	34-38	5	1
α-helix	47-49	3
β-strand	69-76	8	1
β-strand	79-86	8	1
α-helix	96-99	4
β-strand	105-111	7	1
α-helix	118-129	12
β-strand	138-144	7	1
α-helix	146-155	10
α-helix	160-163	4
α-helix	165-167	3
α-helix	184-202	19
β-strand	211-215	5	1
α-helix	223-242	20

Chain B: 4 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-52	6	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	89-94	6	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-153	8	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	175-181	7	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	218-223	6	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
α-helix	272	1
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	303-307	5	8
β-strand	315	1	9
β-strand	319-320	2	2
β-strand	327-328	2	2
β-strand	331	1	9
β-strand	335-339	5	2

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	8-23	16
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chain R: 10 helices, 0 β-strands

Element	Residues	Length
α-helix	49-74	26
α-helix	81-107	27
α-helix	122-150	29
α-helix	161-179	19
α-helix	180-182	3
α-helix	212-226	15
α-helix	228-247	20
α-helix	260-296	37
α-helix	309-333	25
α-helix	335-343	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Bombesin receptor subtype-3,Oplophorus-luciferin 2-monooxygenase catalytic subunit	R	protein	612	Homo sapiens, Oplophorus gracilirostris	P32247 (AlphaFold model), Q9GV45 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-2,Guanine nucleotide-binding protein G(s)…	A	protein	246	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	345	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	70	Bos taurus	P63212

Sequence of entity 1 (R), FASTA

>9LWP_1 Bombesin receptor subtype-3,Oplophorus-luciferin 2-monooxygenase catalytic subunit (chains R)
MKTIIALSYIFCLVFAMAQRQPHSPNQTLISITNDTESSSSVVSNDNTNKGWSGDNSPGI
EALCAIYITYAVIISVGILGNAILIKVFFKTKSMQTVPNIFITSLAFGDLLLLLTCVPVD
ATHYLAEGWLFGRIGCKVLSFIRLTSVGVSVFTLTILSADRYKAVVKPLERQPSNAILKT
CVKAGCVWIVSMIFALPEAIFSNVYTFRDPNKNMTFESCTSYPVSKKLLQEIHSLLCFLV
FYIIPLSIISVYYSLIARTLYKSTLNIPTEEQSHARKQIESRKRIARTVLVLVALFALCW
LPNHLLYLYHSFTSQTYVDPSAMHFIFTIFSRVLAFSNSCVNPFALYWLSKSFQKHFKAQ
LFCCKAERPEPPVADTSLTTLAVMGTVPGTGSIQMSEISVTSFTGCSVKQAEDRFGSSGG
GGSGGGGSSGVFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGE
NALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNY
FGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVTINSGGSENLYFQGGS
AGSAHHHHHHHH

Sequence of entity 2 (A), FASTA

>9LWP_2 Guanine nucleotide-binding protein G(i) subunit alpha-2,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGSTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMRILHGGS
GGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE
ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP
GEDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNL
REYNLV

Sequence of entity 3 (B), FASTA

>9LWP_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (G), FASTA

>9LWP_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
ASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF
REKKFFCAIL

Primary citation

Divergent activation patterns of BRS3 revealed by two Chinese herb-derived agonists. Li, J., Li, C., Zhou, Q. et al. Acta Pharm Sin B (2025) 15:5231-5243. DOI 10.1016/j.apsb.2025.06.025 · PubMed

Other PDB entries of the same protein (UniProt P32247 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9K07 2.83 Å, Cryo-EM structure of the DSO-5a-bound human BRS3-Gq complex
8Y52 2.9 Å, Cryo-EM structure of the BA1-bound BRS3-Gq complex
9M0J 2.9 Å, Cryo-EM structure of Bombesin receptor type-3 bound to oridonin
8Y53 2.93 Å, Cryo-EM structure of the MK-5046-bound BRS3-Gq complex
8Y51 3.3 Å, Cryo-EM structure of the BRS3-Gq complex

Browse structure collections

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