9M1H: PGE2-EP1-Gq complex
Cryo-EM structure of PGE2-EP1-Gq complex. Determined by electron microscopy at 2.55 Å resolution. Released 30 Apr 2025.
- Method
- Electron microscopy
- Resolution
- 2.55 Å
- Organism
- Homo sapiens
- Chains
- 4
- Atoms
- 7,055
- Mol. weight
- 129.7 kDa
- Ligands
- P2E
- Released
- 30 Apr 2025
Explore 9M1H in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9M1H contains 29 α-helices and 36 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 13 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 37-64 | 28 | |
| α-helix | 70-101 | 32 | |
| α-helix | 108-140 | 33 | |
| α-helix | 142-148 | 7 | |
| α-helix | 151-170 | 20 | |
| α-helix | 171-174 | 4 | |
| β-strand | 179-181 | 3 | 1 |
| β-strand | 188-190 | 3 | 1 |
| α-helix | 197-223 | 27 | |
| α-helix | 226-238 | 13 | |
| α-helix | 289-320 | 32 | |
| α-helix | 326-341 | 16 | |
| α-helix | 343-348 | 6 | |
| α-helix | 349-353 | 5 | |
| α-helix | 357-365 | 9 | |
Chain B: 9 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-31 | 24 | |
| β-strand | 33-40 | 8 | 2 |
| α-helix | 46-51 | 6 | |
| β-strand | 184-191 | 8 | 2 |
| β-strand | 194-201 | 8 | 2 |
| α-helix | 211-215 | 5 | |
| β-strand | 220-226 | 7 | 2 |
| α-helix | 230-244 | 15 | |
| β-strand | 254-259 | 6 | 2 |
| α-helix | 261-270 | 10 | |
| α-helix | 275-278 | 4 | |
| α-helix | 280-282 | 3 | |
| α-helix | 299-317 | 19 | |
| β-strand | 326-330 | 5 | 2 |
| α-helix | 338-358 | 21 | |
Chain C: 4 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-29 | 21 | |
| α-helix | 35-37 | 3 | |
| α-helix | 43-44 | 2 | |
| β-strand | 52-56 | 5 | 3 |
| β-strand | 63-68 | 6 | 4 |
| β-strand | 74-79 | 6 | 4 |
| β-strand | 83-88 | 6 | 4 |
| β-strand | 94-99 | 6 | 4 |
| β-strand | 105-110 | 6 | 5 |
| β-strand | 116-121 | 6 | 5 |
| β-strand | 126-130 | 5 | 5 |
| β-strand | 139-144 | 6 | 5 |
| β-strand | 151-156 | 6 | 6 |
| β-strand | 161-166 | 6 | 6 |
| β-strand | 171-175 | 5 | 6 |
| β-strand | 180-184 | 5 | 6 |
| β-strand | 192-197 | 6 | 7 |
| β-strand | 203-208 | 6 | 7 |
| β-strand | 213-217 | 5 | 7 |
| β-strand | 223-227 | 5 | 7 |
| β-strand | 234-239 | 6 | 8 |
| β-strand | 245-250 | 6 | 8 |
| β-strand | 254-259 | 6 | 8 |
| β-strand | 264-270 | 7 | 8 |
| α-helix | 277 | 1 | |
| β-strand | 278-283 | 6 | 9 |
| β-strand | 289-294 | 6 | 9 |
| β-strand | 299-303 | 5 | 9 |
| β-strand | 309-313 | 5 | 9 |
| β-strand | 320-325 | 6 | 3 |
| β-strand | 332-336 | 5 | 3 |
| β-strand | 341-344 | 4 | 3 |
Chain G: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-21 | 15 | |
| α-helix | 29-42 | 14 | |
| α-helix | 52-54 | 3 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Prostaglandin E2 receptor EP1 subtype | A | protein | 402 | Homo sapiens | P34995 (AlphaFold model) |
| Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s)… | B | protein | 361 | Homo sapiens | P50148 (AlphaFold model), P63092 (AlphaFold model), P63096 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | C | protein | 345 | Homo sapiens | P62873 |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Homo sapiens | P59768 |
Sequence of entity 1 (A), FASTA
>9M1H_1 Prostaglandin E2 receptor EP1 subtype (chains A)
MSPCGPLNLSLAGEATTCAAPWVPNTSAVPPSGASPALPIFSMTLGAVSNLLALALLAQA
AGRLRRRRSAATFLLFVASLLATDLAGHVIPGALVLRLYTAGRAPAGGACHFLGGCMVFF
GLCPLLLGCGMAVERCVGVTRPLLHAARVSVARARLALAAVAAVALAVALLPLARVGRYE
LQYPGTWCFIGLGPPGGWRQALLAGLFASLGLVALLAALVCNTLSGLALLRARWRRRSRR
PPPASGPDSRRRWGAHGPRSASASSASSIASASTFFGGSRSSGSARRARAHDVEMVGQLV
GIMVVSCICWSPMLVLVALAVGGWSSTSLQRPLFLAVRLASWNQILDPWVYILLRQAVLR
QLLRLLPPRAGAKGGPAGLGLTPSAWEASSLRSSRHSGLSHF
Sequence of entity 2 (B), FASTA
>9M1H_2 Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(q) subunit alpha (chains B)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRRTLRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRKEFVDISTASGDGRHICYPHFTCSVDTENARRIFNDCKDIILQMNLREYNL
V
Sequence of entity 3 (C), FASTA
>9M1H_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 4 (G), FASTA
>9M1H_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| P2E | (Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]he… | C20 H32 O5 | 1 |
Primary citation
Structural insights into the activation of the human prostaglandin E 2 receptor EP1 subtype by prostaglandin E 2. Meng, X., Li, Y., Xu, J. et al. Proc Natl Acad Sci U S A (2025) 122:e2423840122-e2423840122. DOI 10.1073/pnas.2423840122 · PubMed
Browse structure collections
About this viewer
MolViewer shows 9M1H directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.