9M1I: TBC-D-Arl2-beta-tubulin complex

Cryo-EM structure of the TBC-D-Arl2-beta-tubulin complex. Determined by electron microscopy at 2.48 Å resolution. Released 22 Oct 2025.

Method
Electron microscopy
Resolution
2.48 Å
Organisms
Homo sapiens, Sus scrofa
Chains
3
Atoms
13,518
Mol. weight
203.92 kDa
Ligands
GTP, MG
Released
22 Oct 2025

Explore 9M1I in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9M1I contains 99 α-helices and 44 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain b: 19 helices, 21 β-strands

ElementResiduesLengthSheet
β-strand3-979
α-helix12-2413
β-strand30110
β-strand36110
α-helix44-496
β-strand53111
β-strand59111
β-strand63-6759
α-helix71-766
β-strand90-9239
α-helix101-1022
α-helix103-1075
α-helix109-12618
β-strand130-13679
α-helix143-15816
β-strand163-16759
β-strand169-170212
α-helix181-19212
β-strand198-20039
β-strand202-203212
α-helix204-2096
α-helix210-2145
α-helix222-23615
α-helix250-2578
β-strand265-26629
β-strand267-271513
α-helix286-2927
α-helix296-2983
β-strand299113
β-strand310114
β-strand314115
β-strand315-318413
α-helix323-33311
β-strand350115
β-strand364-369613
β-strand371114
α-helix372-3743
α-helix375-38915
α-helix396-3994
α-helix405-42420
Chain D: 74 helices, 9 β-strands
ElementResiduesLengthSheet
β-strand21-2331
α-helix33-408
α-helix53-6412
α-helix65-673
α-helix71-744
α-helix80-8910
α-helix96-11217
α-helix118-1203
α-helix126-1283
α-helix130-1389
α-helix144-1463
α-helix147-16014
α-helix185-19511
α-helix203-21513
α-helix225-23713
α-helix244-26219
α-helix266-2694
α-helix273-2819
α-helix291-30818
α-helix356-36813
α-helix376-38813
α-helix393-40513
α-helix413-42816
α-helix434-44815
β-strand452-45432
β-strand457-45932
α-helix461-47717
α-helix481-4833
α-helix484-49714
α-helix504-52118
α-helix528-5336
α-helix536-5394
α-helix542-5443
α-helix545-5495
α-helix550-5545
α-helix561-5666
α-helix567-5715
α-helix577-59014
α-helix595-5973
α-helix598-6025
α-helix603-6097
α-helix615-63723
α-helix649-6568
α-helix659-6646
α-helix673-68816
α-helix698-71215
α-helix719-7213
α-helix723-74018
α-helix750-76011
α-helix769-78012
α-helix792-80211
α-helix812-82918
β-strand83113
β-strand84013
α-helix845-8539
β-strand861-86334
β-strand865-86624
α-helix869-88820
α-helix891-8933
α-helix896-90813
α-helix909-9113
α-helix915-92915
β-strand93215
β-strand93515
α-helix9361
α-helix942-9487
α-helix951-9566
α-helix962-9698
α-helix970-9745
α-helix977-9793
α-helix981-99010
α-helix995-100814
α-helix1009-10113
α-helix1016-103217
α-helix1040-105112
α-helix1066-107611
α-helix1085-109713
α-helix1098-11003
α-helix1104-111512
α-helix1116-11183
α-helix1122-113817
α-helix1145-115612
α-helix1164-117815
Chain G: 6 helices, 14 β-strands
ElementResiduesLengthSheet
α-helix4-107
α-helix11-155
β-strand1616
β-strand19-2131
β-strand2311
α-helix27-3610
β-strand50-5451
β-strand5717
β-strand6017
β-strand6116
β-strand63-6751
β-strand7818
β-strand8318
β-strand86-8721
β-strand90-9231
α-helix99-10911
β-strand119-12571
α-helix135-1428
β-strand152-15541
α-helix166-17712

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Tubulin-specific chaperone DDprotein1192Homo sapiensQ9BTW9 (AlphaFold model)
ADP-ribosylation factor-like protein 2Gprotein184Homo sapiensP36404 (AlphaFold model)
Tubulin beta chainbprotein444Sus scrofaQ767L7 (AlphaFold model)
Sequence of entity 1 (D), FASTA
>9M1I_1 Tubulin-specific chaperone D (chains D)
MALSDEPAAGGPEEEAEDETLAFGAALEAFGESAETRALLGRLREVHGGGAEREVALERF
RVIMDKYQEQPHLLDPHLEWMMNLLLDIVQDQTSPASLVHLAFKFLYIITKVRGYKTFLR
LFPHEVADVEPVLDLVTIQNPKDHEAWETRYMLLLWLSVTCLIPFDFSRLDGNLLTQPGQ
ARMSIMDRILQIAESYLIVSDKARDAAAVLVSRFITRPDVKQSKMAEFLDWSLCNLARSS
FQTMQGVITMDGTLQALAQIFKHGKREDCLPYAATVLRCLDGCRLPESNQTLLRKLGVKL
VQRLGLTFLKPKVAAWRYQRGCRSLAANLQLLTQGQSEQKPLILTEDDDEDDDVPEGVER
VIEQLLVGLKDKDTVVRWSAAKGIGRMAGRLPRALADDVVGSVLDCFSFQETDKAWHGGC
LALAELGRRGLLLPSRLVDVVAVILKALTYDEKRGACSVGTNVRDAACYVCWAFARAYEP
QELKPFVTAISSALVIAAVFDRDINCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVG
NRSNCFLVISVFIAGFPEYTQPMIDHLVTMKISHWDGVIRELAARALHNLAQQAPEFSAT
QVFPRLLSMTLSPDLHMRHGSILACAEVAYALYKLAAQENRPVTDHLDEQAVQGLKQIHQ
QLYDRQLYRGLGGQLMRQAVCVLIEKLSLSKMPFRGDTVIDGWQWLINDTLRHLHLISSH
SRQQMKDAAVSALAALCSEYYMKEPGEADPAIQEELITQYLAELRNPEEMTRCGFSLALG
ALPGFLLKGRLQQVLTGLRAVTHTSPEDVSFAESRRDGLKAIARICQTVGVKAGAPDEAV
CGENVSQIYCALLGCMDDYTTDSRGDVGTWVRKAAMTSLMDLTLLLARSQPELIEAHTCE
RIMCCVAQQASEKIDRFRAHAASVFLTLLHFDSPPIPHVPHRGELEKLFPRSDVASVNWS
APSQAFPRITQLLGLPTYRYHVLLGLVVSLGGLTESTIRHSTQSLFEYMKGIQSDPQALG
SFSGTLLQIFEDNLLNERVSVPLLKTLDHVLTHGCFDIFTTEEDHPFAVKLLALCKKEIK
NSKDIQKLLSGIAVFCEMVQFPGDVRRQALLQLCLLLCHRFPLIRKTTASQVYETLLTYS
DVVGADVLDEVVTVLSDTAWDAELAVVREQRNRLCDLLGVPRPQLVPQPGAC
Sequence of entity 2 (G), FASTA
>9M1I_2 ADP-ribosylation factor-like protein 2 (chains G)
MGLLTILKKMKQKERELRLLMLGLDNAGKTTILKKFNGEDIDTISPTLGFNIKTLEHRGF
KLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEERLAGATL
LIFANKQDLPGALSSNAIREVLELDSIRSHHWCIQGCSAVTGENLLPGIDWLLDDISSRI
FTAD
Sequence of entity 3 (b), FASTA
>9M1I_3 Tubulin beta chain (chains b)
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYV
PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVV
EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMM
AACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRG
LKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATAEEEEDFGEEAEEEA

Ligands and cofactors

IDNameFormulaCopies
GTPGuanosine-5'-triphosphateC10 H16 N5 O14 P31
MGMagnesium ionMg1

Primary citation

Structural dissection of alpha beta-tubulin heterodimer assembly and disassembly by human tubulin-specific chaperones. Seong, Y., Kim, H., Byun, K. et al. Science (2025) 390:eady2708-eady2708. DOI 10.1126/science.ady2708 · PubMed

Other PDB entries of the same protein (UniProt Q9BTW9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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