9PIS: Ab initio structure of crambin by MicroED

Ab initio structure of crambin by MicroED at 0.85A. Determined by electron crystallography at 0.85 Å resolution. Released 25 Feb 2026.

Method: Electron crystallography
Resolution: 0.85 Å
Organism: Crambe hispanica subsp. abyssinica
Chains: 1
Atoms: 435
Mol. weight: 4.73 kDa
Released: 25 Feb 2026

Explore 9PIS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9PIS contains 2 α-helices and 2 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 2 helices, 2 β-strands

Element	Residues	Length	Sheet
β-strand	2-3	2	1
α-helix	7-17	11
α-helix	23-30	8
β-strand	33-34	2	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Crambin	A	protein	46	Crambe hispanica subsp. abyssinica	P01542 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9PIS_1 Crambin (chains A)
TTCCPSIVARSNFNVCRLPGTSEAICATYTGCIIIPGATCPGDYAN

Primary citation

Direct from the seed: an atomic resolution protein structure by ab initio MicroED. Vasireddy, P.C.R., Low-Beer, T., Spoth, K.A. et al. Nat Commun (2026) 17. DOI 10.1038/s41467-026-69601-y · PubMed

Other PDB entries of the same protein (UniProt P01542 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3NIR 0.48 Å, Crystal structure of small protein crambin at 0.48 A resolution
1EJG 0.54 Å, Crambin at ultra-high resolution: valence electron density.
9EWK 0.7 Å, Solvent organization in ultrahigh-resolution protein crystal structure at room temperature
1CBN 0.83 Å, Atomic resolution (0.83 Å) crystal structure of the hydrophobic protein crambin at 130 K
3U7T 0.85 Å, Room temperature ultra-high resolution time-of-flight neutron and X-ray diffraction…
1AB1 0.89 Å, Si form crambin
1JXT 0.89 Å, Crambin mixed sequence form at 160 K. Protein/water substates
1JXW 0.89 Å, Crambin mixed sequence form at 180 K. Protein/water substates
1JXX 0.89 Å, Crambin mixed sequence form at 200 K. Protein/water substates
1JXY 0.89 Å, Crambin mixed sequence form at 220 K. Protein/water substates
1JXU 0.99 Å, Crambin mixed sequence form at 240 K. Protein/water substates
1CNR 1.05 Å, Correlated disorder of the pure PRO22(SLASH)LEU25 form of crambin at 150K refined to…

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