9R2I: Complex CDK16:CCNY:14-3-3

Cryo-EM structure of the complex CDK16:CCNY:14-3-3. Determined by electron microscopy at 3.3 Å resolution. Released 25 Mar 2026.

Method
Electron microscopy
Resolution
3.3 Å
Organism
Homo sapiens
Chains
4
Atoms
9,145
Mol. weight
142.53 kDa
Ligands
AGS, MG
Released
25 Mar 2026

Explore 9R2I in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9R2I contains 63 α-helices and 18 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 0 β-strands

ElementResiduesLengthSheet
α-helix6-1611
α-helix20-3213
α-helix39-7335
α-helix76-10328
α-helix104-1085
α-helix117-13721
α-helix141-16121
α-helix170-18112
α-helix182-1865
α-helix190-20617
α-helix213-2153
α-helix216-23318
Chain B: 12 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix4-1714
α-helix20-3213
α-helix39-7335
α-helix76-10328
α-helix104-1096
α-helix110-1112
α-helix117-13519
α-helix141-16424
α-helix170-18112
α-helix182-1865
α-helix190-20617
α-helix218-23417
Chain C: 21 helices, 14 β-strands
ElementResiduesLengthSheet
α-helix111-1188
α-helix131-1355
α-helix147-15711
α-helix162-1643
β-strand165-17281
β-strand177-18481
β-strand190-19671
β-strand19912
β-strand20312
α-helix206-21510
β-strand22313
β-strand226-22941
β-strand238-24141
β-strand245-24624
α-helix247-2537
α-helix260-27920
β-strand282-28325
α-helix289-2913
β-strand293-29424
β-strand300-30124
β-strand30213
β-strand309-31025
α-helix318-3203
α-helix330-3334
α-helix342-35716
α-helix367-37812
α-helix393-3986
α-helix408-4114
α-helix417-42610
α-helix431-4333
α-helix435-4362
α-helix437-4404
α-helix444-4463
α-helix452-4554
α-helix462-4643
Chain D: 18 helices, 4 β-strands
ElementResiduesLengthSheet
α-helix41-433
α-helix44-485
α-helix55-573
α-helix68-747
β-strand10516
α-helix116-13217
α-helix156-1583
α-helix165-1673
α-helix168-18215
α-helix186-20318
α-helix213-22816
α-helix246-26015
α-helix268-28316
α-helix2921
β-strand29316
α-helix2941
β-strand29517
α-helix296-3027
α-helix306-3094
α-helix314-3185
α-helix323-3253
β-strand33717

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
14-3-3 protein etaA, Bprotein248Homo sapiensQ04917 (AlphaFold model)
Cyclin-dependent kinase 16Cprotein395Homo sapiensQ00536 (AlphaFold model)
Cyclin-YDprotein346Homo sapiensQ8ND76 (AlphaFold model)
Sequence of entity 1 (A, B), FASTA
>9R2I_1 14-3-3 protein eta (chains A, B)
GHMGDREQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRS
SWRVISSIEQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQYE
SKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQPTHPIRLGLALN
FSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQ
DEEAGEGN
Sequence of entity 2 (C), FASTA
>9R2I_2 Cyclin-dependent kinase 16 (chains C)
GPLGSRKISTEDINKRLSLPADIRLPEGYLEKLTLNSPIFDKPLSRRLRRVSLSEIGFGK
LETYIKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHA
NIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNIINMHNVKLFLFQLLRGLAYCHRQK
VLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDILLGSTDYST
QIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEEFKTYNYP
KYRAEALLSHAPRLDSDGADLLTKLLQFEGRNRISAEDAMKHPFFLSLGERIHKLPDTTS
IFALKEIQLQKEASLRSSSMPDSGRPAFRVVDTEF
Sequence of entity 3 (D), FASTA
>9R2I_3 Cyclin-Y (chains D)
GPLGSMGNTTSCCVSSSPKLRRNAHSRLESYRPDTDLSREDTGCNLQHISDRENIDDLNM
EFNPSDHPRASTIFLSKSQTDVREKRKSLFINHHPPGQIARKRASCSTIFLDDSTVSQPN
LKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHPLSKSEVPPDYDKHNPEQKQIYRFV
RTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPANWKRIVLGAILLASKVWDDQAVWNV
DYCQILKDITVEDMNELERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLS
RERAHKLEAISRLCEDKYKDLRRSARKRAASADNLTLPRWSPAIIS

Ligands and cofactors

IDNameFormulaCopies
AGSPhosphothiophosphoric acid-adenylate esterC10 H16 N5 O12 P3 S1
MGMagnesium ionMg1

Primary citation

Structural basis of the cyclin Y/14-3-3 protein-mediated activation of CDK16. Kohoutova, K., Kosek, D., Brzezina, A. et al. Nat Commun (2026) 17. DOI 10.1038/s41467-026-70778-5 · PubMed

Other PDB entries of the same protein (UniProt Q04917 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 9R2I directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.