9UWH: Human mitoribosome trapped by retapamulin

Name: PDB 9UWH: human mitoribosome trapped by retapamulin
Published: 2025-10-29
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: ribosome

human mitoribosome trapped by retapamulin. Determined by electron microscopy at 3.0 Å resolution. Released 29 Oct 2025.

Method: Electron microscopy
Resolution: 3.0 Å
Organism: Homo sapiens
Chains: 86
Atoms: 171,472
Mol. weight: 2939.79 kDa
Ligands: GDP, ATP, FES, ZN
Released: 29 Oct 2025

Explore 9UWH in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9UWH contains 774 α-helices and 565 β-strands across 81 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 0: 5 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	84-86	3
α-helix	87-94	8
α-helix	101-104	4
β-strand	107-109	3	46
β-strand	116-118	3	46
α-helix	124-145	22
β-strand	156-159	4	47
β-strand	173-176	4	47
α-helix	180-182	3

Chain 1: 1 helix, 4 β-strands

Element	Residues	Length	Sheet
β-strand	15-22	8	55
β-strand	29-35	7	55
α-helix	39-41	3
β-strand	42-47	6	55
β-strand	52-64	13	55

Chain 2: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	57-63	7
α-helix	66-71	6
α-helix	73-85	13

Chain 3: 6 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	108-113	6
β-strand	115-116	2	67
β-strand	122-125	4	67
α-helix	133-135	3
α-helix	138-144	7
β-strand	147-149	3	67
α-helix	152-161	10
α-helix	164-166	3
β-strand	172	1	76
α-helix	178-180	3

Chain 4: 1 helix, 4 β-strands

Element	Residues	Length	Sheet
β-strand	68-69	2	75
β-strand	80-84	5	75
β-strand	87-91	5	75
α-helix	96-98	3
β-strand	100-102	3	75

Chain 5: 20 helices, 20 β-strands

Element	Residues	Length	Sheet
α-helix	40-47	8
α-helix	48-51	4
β-strand	59	1	80
β-strand	71	1	80
α-helix	72-73	2
α-helix	75-77	3
α-helix	85-87	3
α-helix	90-92	3
α-helix	93-95	3
β-strand	99-107	9	81
α-helix	117-124	8
β-strand	126-130	5	82
α-helix	134-139	6
α-helix	147	1
α-helix	150-162	13
α-helix	173-189	17
α-helix	196-200	5
β-strand	201-205	5	82
β-strand	209-214	6	81
β-strand	219-224	6	81
β-strand	228-232	5	82
α-helix	235-238	4
α-helix	242-247	6
α-helix	248-250	3
α-helix	253-255	3
β-strand	259	1	19
β-strand	266-270	5	81
β-strand	276	1	81
β-strand	279	1	83
β-strand	287-294	8	82
α-helix	304-305	2
α-helix	306-329	24
β-strand	335-347	13	82
β-strand	351-361	11	82
β-strand	372-381	10	82
β-strand	385-387	3	84
β-strand	391-393	3	85
β-strand	396-399	4	85
β-strand	402-404	3	84
α-helix	408-419	12

Chain 6: 22 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	34-35	2
β-strand	50	1	150
α-helix	54-66	13
α-helix	74-77	4
α-helix	86-88	3
β-strand	89	1	93
α-helix	92-95	4
α-helix	99-115	17
α-helix	117-125	9
α-helix	132-142	11
α-helix	144-155	12
α-helix	157-160	4
β-strand	166	1	93
β-strand	173-179	7	94
β-strand	183-186	4	94
β-strand	192	1	95
α-helix	201-202	2
β-strand	203-206	4	94
β-strand	213-220	8	95
β-strand	231-241	11	95
α-helix	245-247	3
β-strand	249-252	4	95
α-helix	259-260	2
β-strand	261	1	96
β-strand	266-275	10	95
α-helix	293-296	4
β-strand	297-298	2	95
α-helix	300-306	7
α-helix	308-310	3
β-strand	313-321	9	95
α-helix	325-329	5
α-helix	330-334	5
α-helix	338-339	2
β-strand	340-345	6	96
α-helix	346-350	5
α-helix	352-354	3
β-strand	359	1	67
β-strand	362	1	67
α-helix	364-369	6

Chain 7: 12 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	37-58	22
β-strand	64-69	6	101
β-strand	78-82	5	101
α-helix	88-94	7
α-helix	97-102	6
β-strand	105-108	4	101
β-strand	111-113	3	101
α-helix	118-119	2
β-strand	123-128	6	101
α-helix	136-156	21
β-strand	165-168	4	102
α-helix	174-176	3
β-strand	179-184	6	102
α-helix	187-190	4
α-helix	196-212	17
β-strand	215	1	103
β-strand	216-222	7	104
α-helix	223-229	7
α-helix	234-246	13
β-strand	251-257	7	104
β-strand	260-263	4	104
β-strand	271	1	103
α-helix	272-274	3
β-strand	275-285	11	102
β-strand	294-303	10	102
α-helix	310-320	11

73 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
16S mitochondrial rRNA	A	RNA	1561	Homo sapiens
mitochondrial tRNAVal	B	RNA	72	Homo sapiens
Large ribosomal subunit protein uL2m	D	protein	305	Homo sapiens	Q5T653 (AlphaFold model)
Large ribosomal subunit protein uL3m	E	protein	348	Homo sapiens	P09001 (AlphaFold model)
Large ribosomal subunit protein uL4m	F	protein	311	Homo sapiens	Q9BYD3 (AlphaFold model)
Large ribosomal subunit protein bL9m	H	protein	267	Homo sapiens	Q9BYD2 (AlphaFold model)
Large ribosomal subunit protein uL10m	I	protein	261	Homo sapiens	Q7Z7H8
Large ribosomal subunit protein uL11m	J	protein	192	Homo sapiens	Q9Y3B7
Large ribosomal subunit protein uL13m	K	protein	178	Homo sapiens	Q9BYD1
Large ribosomal subunit protein uL14m	L	protein	145	Homo sapiens	Q6P1L8
Large ribosomal subunit protein uL15m	M	protein	296	Homo sapiens	Q9P015
Large ribosomal subunit protein uL16m	N	protein	251	Homo sapiens	Q9NX20

74 more molecules are not listed.

Sequence of entity 1 (A), FASTA

>9UWH_1 16S mitochondrial rRNA (chains A)
GCUAAACCUAGCCCCAAACCCACUCCACCUUACUACCAGACAACCUUAGCCAAACCAUUU
ACCCAAAUAAAGUAUAGGCGAUAGAAAUUGAAACCUGGCGCAAUAGAUAUAGUACCGCAA
GGGAAAGAUGAAAAAUUAUAACCAAGCAUAAUAUAGCAAGGACUAACCCCUAUACCUUCU
GCAUAAUGAAUUAACUAGAAAUAACUUUGCAAGGAGAGCCAAAGCUAAGACCCCCGAAAC
CAGACGAGCUACCUAAGAACAGCUAAAAGAGCACACCCGUCUAUGUAGCAAAAUAGUGGG
AAGAUUUAUAGGUAGAGGCGACAAACCUACCGAGCCUGGUGAUAGCUGGUUGUCCAAGAU
AGAAUCUUAGUUCAACUUUAAAUUUGCCCACAGAACCCUCUAAAUCCCCUUGUAAAUUUA
ACUGUUAGUCCAAAGAGGAACAGCUCUUUGGACACUAGGAAAAAACCUUGUAGAGAGAGU
AAAAAAUUUAACACCCAUAGUAGGCCUAAAAGCAGCCACCAAUUAAGAAAGCGUUCAAGC
UCAACACCCACUACCUAAAAAAUCCCAAACAUAUAACUGAACUCCUCACACCCAAUUGGA
CCAAUCUAUCACCCUAUAGAAGAACUAAUGUUAGUAUAAGUAACAUGAAAACAUUCUCCU
CCGCAUAAGCCUGCGUCAGAUUAAAACACUGAACUGACAAUUAACAGCCCAAUAUCUACA
AUCAACCAACAAGUCAUUAUUACCCUCACUGUCAACCCAACACAGGCAUGCUCAUAAGGA
AAGGUUAAAAAAAGUAAAAGGAACUCGGCAAAUCUUACCCCGCCUGUUUACCAAAAACAU
CACCUCUAGCAUCACCAGUAUUAGAGGCACCGCCUGCCCAGUGACACAUGUUUAACGGCC
GCGGUACCCUAACCGUGCAAAGGUAGCAUAAUCACUUGUUCCUUAAAUAGGGACCUGUAU
GAAUGGCUCCACGAGGGUUCAGCUGUCUCUUACUUUUAACCAGUGAAAUUGACCUGCCCG
UGAAGAGGCGGGCAUAACACAGCAAGACGAGAAGACCCUAUGGAGCUUUAAUUUAUUAAU
GCAAACAGUACCUAACAAACCCACAGGUCCUAAACUACCAAACCUGCAUUAAAAAUUUCG
GUUGGGGCGACCUCGGAGCAGAACCCAACCUCCGAGCAGUACAUGCUAAGACUUCACCAG
UCAAAGCGAACUACUAUACUCAAUUGAUCCAAUAACUUGACCAACGGAACAAGUUACCCU
AGGGAUAACAGCGCAAUCCUAUUCUAGAGUCCAUAUCAACAAUAGGGUUUACGACCUCGA
UGUUGGAUCAGGACAUCCCGAUGGUGCAGCCGCUAUUAAAGGUUCGUUUGUUCAACGAUU
AAAGUCCUACGUGAUCUGAGUUCAGACCGGAGUAAUCCAGGUCGGUUUCUAUCUACUUUC
AAAUUCCUCCCUGUACGAAAGGACAAGAGAAAUAAGGCCUACUUCACAAAGCGCCUUCCC
CCGUAAAUGAUAUCAUCUCAACUUAGUAUUAUACCCACACCCACCCAAGAACAGGGUUUG
U

Sequence of entity 2 (B), FASTA

>9UWH_2 mitochondrial tRNAVal (chains B)
CAGAGUGUAGCUUAACACAAAGCACCCAACUUACACUUAGGAGAUUUCAACUUAACUUGA
CCGCUCUGACCA

Sequence of entity 3 (D), FASTA

>9UWH_3 Large ribosomal subunit protein uL2m (chains D)
MALCALTRALRSLNLAPPTVAAPAPSLFPAAQMMNNGLLQQPSALMLLPCRPVLTSVALN
ANFVSWKSRTKYTITPVKMRKSGGRDHTGRIRVHGIGGGHKQRYRMIDFLRFRPEETKSG
PFEEKVIQVRYDPCRSADIALVAGGSRKRWIIATENMQAGDTILNSNHIGRMAVAAREGD
AHPLGALPVGTLINNVESEPGRGAQYIRAAGTCGVLLRKVNGTAIIQLPSKRQMQVLETC
VATVGRVSNVDHNKRVIGKAGRNRWLGKRPNSGRWHRKGGWAGRKIRPLPPMKSYVKLPS
ASAQS

Sequence of entity 4 (E), FASTA

>9UWH_4 Large ribosomal subunit protein uL3m (chains E)
MPGWRLLTQVGAQVLGRLGDGLGAALGPGNRTHIWLFVRGLHGKSGTWWDEHLSEENVPF
IKQLVSDEDKAQLASKLCPLKDEPWPIHPWEPGSFRVGLIALKLGMMPLWTKDGQKHVVT
LLQVQDCHVLKYTSKENCNGKMATLSVGGKTVSRFRKATSILEFYRELGLPPKQTVKIFN
ITDNAAIKPGTPLYAAHFRPGQYVDVTAKTIGKGFQGVMKRWGFKGQPATHGQTKTHRRP
GAVATGDIGRVWPGTKMPGKMGNIYRTEYGLKVWRINTKHNIIYVNGSVPGHKNCLVKVK
DSKLPAYKDLGKNLPFPTYFPDGDEEELPEDLYDENVCQPGAPSITFA

Sequence of entity 5 (F), FASTA

>9UWH_5 Large ribosomal subunit protein uL4m (chains F)
MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRR
PVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEV
RGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTV
KLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTF
NLIPAVGLNVHSMLKHQTLVLTLPTVAFLEDKLLWQDSRYRPLYPFSLPYSDFPRPLPHA
TQGPAATPYHC

Sequence of entity 6 (H), FASTA

>9UWH_6 Large ribosomal subunit protein bL9m (chains H)
MAAPVVTAPGRALLRAGAGRLLRGGVQELLRPRHEGNAPDLACNFSLSQNRGTVIVERWW
KVPLAGEGRKPRLHRRHRVYKLVEDTKHRPKENLELILTQSVENVGVRGDLVSVKKSLGR
NRLLPQGLAVYASPENKKLFEEEKLLRQEGKLEKIQTKAGEATVKFLKSCRLEVGMKNNV
KWELNPEIVARHFFKNLGVVVAPHTLKLPEEPITRWGEYWCEVTVNGLDTVRVPMSVVNF
EKPKTKRYKYWLAQQAAKAMAPTSPQI

Sequence of entity 7 (I), FASTA

>9UWH_7 Large ribosomal subunit protein uL10m (chains I)
MAAAVAGMLRGGLLPQAGRLPTLQTVRYGSKAVTRHRRVMHFQRQKLMAVTEYIPPKPAI
HPSCLPSPPSPPQEEIGLIRLLRREIAAVFQDNRMIAVCQNVALSAEDKLLMRHQLRKHK
ILMKVFPNQVLKPFLEDSKYQNLLPLFVGHNMLLVSEEPKVKEMVRILRTVPFLPLLGGC
IDDTILSRQGFINYSKLPSLPLVQGELVGGLTCLTAQTHSLLQHQPLQLTTLLDQYIREQ
REKDSVMSANGKPDPDTVPDS

Sequence of entity 8 (J), FASTA

>9UWH_8 Large ribosomal subunit protein uL11m (chains J)
MSKLGRAARGLRKPEVGGVIRAIVRAGLAMPGPPLGPVLGQRGVSINQFCKEFNERTKDI
KEGIPLPTKILVKPDRTFEIKIGQPTVSYFLKAAAGIEKGARQTGKEVAGLVTLKHVYEI
ARIKAQDEAFALQDVPLSSVVRSIIGSARSLGIRVVKDLSSEELAAFQKERAIFLAAQKE
ADLAAQEEAAKK

Sequence of entity 9 (K), FASTA

>9UWH_9 Large ribosomal subunit protein uL13m (chains K)
XSSFSRAPQQWATFARIWYLLDGKMQPPGKLAAMASIRLQGLHKPVYHALSDCGDHVVIM
NTRHIAFSGNKWEQKVYSSHTGYPGGFRQVTAAQLHLRDPVAIVKLAIYGMLPKNLHRRT
MMERLHLFPDEYIPEDILKNLVEELPQPRKIPKRLDEYTQEEIDAFPRLWTPPEDYRL

Sequence of entity 10 (L), FASTA

>9UWH_10 Large ribosomal subunit protein uL14m (chains L)
MAFFTGLWGPFTCVSRVLSHHCFSTTGSLSAIQKMTRVRVVDNSALGNSPYHRAPRCIHV
YKKNGVGKVGDQILLAIKGQKKKALIVGHCMPGPRMTPRFDSNNVVLIEDNGNPVGTRIK
TPIPTSLRKREGEYSKVLAIAQNFV

Sequence of entity 11 (M), FASTA

>9UWH_11 Large ribosomal subunit protein uL15m (chains M)
MAGPLQGGGARALDLLRGLPRVSLANLKPNPGSKKPERRPRGRRRGRKCGRGHKGERQRG
TRPRLGFEGGQTPFYIRIPKYGFNEGHSFRRQYKPLSLNRLQYLIDLGRVDPSQPIDLTQ
LVNGRGVTIQPLKRDYGVQLVEEGADTFTAKVNIEVQLASELAIAAIEKNGGVVTTAFYD
PRSLDIVCKPVPFFLRGQPIPKRMLPPEELVPYYTDAKNRGYLADPAKFPEARLELARKY
GYILPDITKDELFKMLCTRKDPRQIFFGLAPGWVVNMADKKILKPTDENLLKYYTS

Sequence of entity 12 (N), FASTA

>9UWH_12 Large ribosomal subunit protein uL16m (chains N)
MWRLLARASAPLLRVPLSDSWALLPASAGVKTLLPVPSFEDVSIPEKPKLRFIERAPLVP
KVRREPKNLSDIRGPSTEATEFTEGNFAILALGGGYLHWGHFEMMRLTINRSMDPKNMFA
IWRVPAPFKPITRKSVGHRMGGGKGAIDHYVTPVKAGRLVVEMGGRCEFEEVQGFLDQVA
HKLPFAAKAVSRGTLEKMRKDQEERERNNQNPWTFERIATANMLGIRKVLSPYDLTHKGK
YWGKFYMPKRV

Ligands and cofactors

ID	Name	Formula	Copies
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	1
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	1
FES	FE2/S2 (inorganic) cluster	Fe2 S2	3
ZN	Zinc ion	Zn	3
SPD	Spermidine	C7 H19 N3	1
NAD	Nicotinamide-adenine-dinucleotide	C21 H27 N7 O14 P2	1
VAL	Valine	C5 H11 N O2	1
G34	Retapamulin	C30 H47 N O4 S	1
MG	Magnesium ion	Mg	208

Water and common crystallization additives (K) are not listed.

Primary citation

Monitoring the complexity and dynamics of mitochondrial translation. Wakigawa, T., Mito, M., Ando, Y. et al. Mol Cell (2025) 85:4279-4297.e8. DOI 10.1016/j.molcel.2025.10.022 · PubMed

Other PDB entries of the same protein (UniProt Q5T653 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7OF0 2.2 Å, Structure of a human mitochondrial ribosome large subunit assembly intermediate in…
7QI4 2.21 Å, Human mitochondrial ribosome at 2.2 A resolution (bound to partly built tRNAs and mRNA)
8RRI 2.4 Å, Human mitochondrial ribosome in complex with antibiotic tigecycline
8QU5 2.42 Å, mt-LSU assembly intermediate in GTPBP8 knock-out cells, state 2
9OLF 2.46 Å, Membrane-associated human mitoribosome in complex with TACO1
7OF7 2.5 Å, Structure of a human mitochondrial ribosome large subunit assembly intermediate in…
7PO4 2.56 Å, Assembly intermediate of human mitochondrial ribosome large subunit (largely unfolded…
6ZM6 2.59 Å, Human mitochondrial ribosome in complex with mRNA, A/A tRNA and P/P tRNA
7O9M 2.6 Å, Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4,…
7OF6 2.6 Å, Structure of mature human mitochondrial ribosome large subunit in complex with GTPBP6…
9CN3 2.62 Å, Human 39S mitoribosome in complex with antibiotic Linezolid
7QI5 2.63 Å, Human mitochondrial ribosome in complex with mRNA, A/A-, P/P- and E/E-tRNAs at 2.63 A…

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