9WG0: D1R-Gs in

D1R-Gs in complexed with SKF81297/LY3154207/BMSA1/UNC10062. Determined by electron microscopy at 2.59 Å resolution. Released 26 Aug 2026.

Method: Electron microscopy
Resolution: 2.59 Å
Organisms: Homo sapiens, Rattus norvegicus, Bos taurus
Chains: 5
Atoms: 8,335
Mol. weight: 162.93 kDa
Ligands: A1EV3, G4C, SK0, A1EWL
Released: 26 Aug 2026

Explore 9WG0 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9WG0 contains 38 α-helices and 52 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	12-38	27
β-strand	40-47	8	1
α-helix	53-55	3
β-strand	62-65	4	1
β-strand	70-75	6	1
β-strand	80-85	6	1
α-helix	96-100	5
β-strand	105-111	7	1
α-helix	115-129	15
β-strand	138-144	7	1
α-helix	146-155	10
α-helix	160-162	3
α-helix	165-168	4
α-helix	184-204	21
β-strand	211-215	5	1
α-helix	223-242	20

Chain B: 5 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	4-23	20
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	89-94	6	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	175-180	6	5
α-helix	186	1
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	249-254	6	7
β-strand	262-264	3	7
α-helix	272	1
β-strand	273	1	8
β-strand	277-278	2	9
β-strand	284-288	5	9
β-strand	289	1	8
β-strand	294-298	5	9
β-strand	304-307	4	9
β-strand	309	1	10
β-strand	311	1	10
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	30-43	14
α-helix	53-55	3

Chain N: 3 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	10-11	2	12
β-strand	17-25	9	11
α-helix	29-31	3
β-strand	34-39	6	12
β-strand	46-50	5	12
β-strand	59-60	2	12
β-strand	69-73	5	11
α-helix	74-76	3
β-strand	78-84	7	11
α-helix	88-90	3
β-strand	92-98	7	12
β-strand	118	1	12
β-strand	122-125	4	12

Chain R: 18 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	22-50	29
α-helix	52-56	5
α-helix	60-76	17
α-helix	78-87	10
α-helix	93-95	3
α-helix	96-126	31
α-helix	128-134	7
α-helix	137-160	24
β-strand	165	1	13
α-helix	166-167	2
α-helix	189	1
β-strand	190	1	13
α-helix	191	1
α-helix	192-199	8
α-helix	200-204	5
α-helix	205-239	35
α-helix	265-292	28
α-helix	295-297	3
α-helix	310-331	22
α-helix	335-344	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	246	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	371	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	68	Bos taurus	P63212 (AlphaFold model)
Nanobody35	N	protein	160	Vicugna pacos
Beta-2 adrenergic receptor,D(1A) dopamine receptor,Engineered oplophorus-luciferin 2-monooxygenase…	R	protein	603	Homo sapiens, Oplophorus gracilirostris	P07550 (AlphaFold model), P21728

Sequence of entity 1 (A), FASTA

>9WG0_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQM
RIYHVNSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE
ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP
GEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHL
RQYELL

Sequence of entity 2 (B), FASTA

>9WG0_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSG
VSGWRLFKKIS

Sequence of entity 3 (G), FASTA

>9WG0_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFC

Sequence of entity 4 (N), FASTA

>9WG0_4 Nanobody35 (chains N)
MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVR
QAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCAR
CPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHHEPEA

Sequence of entity 5 (R), FASTA

>9WG0_5 Beta-2 adrenergic receptor,D(1A) dopamine receptor,Engineered oplophorus-luciferin 2-monooxygenase catalytic subunit (chains R)
MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSDYKDDDDVDMGQPGNGSAFLLAPNGSHAPD
HDVTQQRDEASRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVCAAVIR
FRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTAS
ILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSWHKAKPTS
PSDGNATSLAETIDNCDSSLSRTYAISSSVISFYIPVAIMIVTYTRIYRIAQKQIRRIAA
LERAAVHAKNCQTTTGNGKPVECSQPESSFKMSFKRETKVLKTLSVIMGVFVCCWLPFFI
LNCILPFCGSGETQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFSTLLGCYRL
CPATNNAIETVSINNNGVFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQ
RIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVIDGV
TPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVTINSHHHHH
HHH

Ligands and cofactors

ID	Name	Formula	Copies
A1EV3	1-[(1~{S},3~{R},6~{R})-6-(1,3-benzodioxol-5-yl)-3-bicyclo[4.1.0]heptanyl]-4-[(2…	C25 H28 Br Cl N2 O2	1
G4C	2-[2,6-bis(chloranyl)phenyl]-1-[(1S,3R)-3-(hydroxymethyl)-1-methyl-5-(3-methyl-…	C24 H29 Cl2 N O3	1
SK0	(1R)-6-chloro-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine-7,8-diol	C16 H16 Cl N O2	1
A1EWL	methyl 2-[(6~{R})-3-aminocarbonyl-2-(pyridin-4-ylcarbonylamino)-4,5,6,7-tetrahy…	C20 H23 N3 O4 S	1
PLM	Palmitic acid	C16 H32 O2	1

Primary citation

Molecular insights into the cooperative allosteric modulation of D1 dopamine receptor. Xu, Y., Wang, K., Zhuang, Y. To be published.

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

Browse structure collections

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