9Y7H: Gb1g2 crosslinked to PLCb3

Gb1g2 crosslinked to PLCb3. Determined by electron microscopy at 4.4 Å resolution. Released 18 Feb 2026.

Method: Electron microscopy
Resolution: 4.4 Å
Organisms: Homo sapiens, Bos taurus
Chains: 3
Atoms: 8,913
Mol. weight: 144.26 kDa
Ligands: CA, ME7
Released: 18 Feb 2026

Explore 9Y7H in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9Y7H contains 43 α-helices and 74 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 38 helices, 41 β-strands

Element	Residues	Length	Sheet
α-helix	20-24	5
β-strand	28-29	2	1
β-strand	32	1	2
β-strand	37	1	2
β-strand	41	1	1
β-strand	44-45	2	3
β-strand	51-55	5	3
β-strand	61-65	5	3
α-helix	66-68	3
β-strand	69-74	6	1
α-helix	84-89	6
α-helix	100-102	3
β-strand	103-108	6	1
β-strand	116-122	7	1
α-helix	128-139	12
α-helix	142-145	4
α-helix	149-162	14
β-strand	170-171	2	4
α-helix	172-178	7
α-helix	183-193	11
β-strand	202-203	2	4
α-helix	205-207	3
α-helix	210-218	9
α-helix	224-233	10
β-strand	241-242	2	5
α-helix	243-252	10
α-helix	265-268	4
α-helix	269-278	10
β-strand	290-291	2	5
α-helix	293-301	9
α-helix	310-313	4
β-strand	326	1	6
β-strand	344	1	7
α-helix	348-354	7
β-strand	363	1	8
β-strand	365-366	2	9
α-helix	367-369	3
β-strand	376-377	2	9
β-strand	385	1	7
β-strand	387-388	2	9
α-helix	389-396	8
α-helix	397-399	3
β-strand	412	1	8
α-helix	419-431	13
α-helix	434-436	3
β-strand	437	1	10
α-helix	441-442	2
α-helix	452-455	4
α-helix	456-459	4
β-strand	463	1	10
α-helix	578-581	4
α-helix	588-591	4
β-strand	599	1	11
α-helix	605-611	7
β-strand	617	1	11
α-helix	622-631	10
α-helix	633-640	8
β-strand	644	1	11
β-strand	647	1	12
α-helix	662-666	5
β-strand	672	1	12
α-helix	681-690	10
β-strand	700	1	6
α-helix	701-702	2
α-helix	703-706	4
β-strand	726-733	8	13
β-strand	734	1	14
β-strand	735-736	2	13
β-strand	745-750	6	15
α-helix	765-768	4
β-strand	775	1	14
α-helix	779-781	3
β-strand	783-786	4	13
β-strand	793-800	8	15
β-strand	805-812	8	15
β-strand	823	1	13
β-strand	826	1	15
β-strand	837-845	9	13
α-helix	869-877	9

Chain B: 3 helices, 33 β-strands

Element	Residues	Length	Sheet
α-helix	9-25	17
α-helix	30-36	7
α-helix	38-39	2
β-strand	49	1	16
β-strand	61-63	3	17
β-strand	69-72	4	17
β-strand	74	1	18
β-strand	78	1	18
β-strand	80-83	4	17
β-strand	91-92	2	17
β-strand	105	1	19
β-strand	112	1	19
β-strand	123-124	2	19
β-strand	135-137	3	19
β-strand	146-153	8	20
β-strand	156-161	6	20
β-strand	165-170	6	20
β-strand	176-181	6	20
β-strand	187-192	6	21
β-strand	198	1	22
β-strand	199-203	5	21
β-strand	208-211	4	21
β-strand	212	1	22
β-strand	218-221	4	21
β-strand	229-234	6	23
β-strand	240-245	6	23
β-strand	249-254	6	23
β-strand	259	1	23
β-strand	263-264	2	23
β-strand	273-278	6	24
β-strand	284-289	6	24
β-strand	294-298	5	24
β-strand	304-308	5	24
β-strand	315-320	6	16
β-strand	327-331	5	16
β-strand	337-338	2	16

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	11-23	13
α-helix	30-45	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3	A	protein	883	Homo sapiens	Q01970 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	332	Bos taurus	P62871 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9Y7H_1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (chains A)
MAHHHHHHGTALQLEPPTVVETLRRGSKFIKWDEETSSRNLVTLRVDPNGFFLYWTGPNM
CVDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDARLEEKLMTVVSGPDPVNTVFLN
FMAVQDDTAKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMF
SADKKRVETALESSGLKFNRSESIRPDEFSLEIFERFLNKLSLRPDIDKILLEIGAKGKP
YLTLEQLMDFINQKQRDPRLNEVLYPPLRPSQARLLIEKYEPNQQFLERDQMSMEGFSRY
LGGEENGILPLEALDLSTDMTQPLSAYFINSSHNTYLTAGQLAGTSSVEMYRQALLWGSR
CVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAETAFKTSPYPVILSFENHVDSA
KQQAKMAEYCRSIFGDALLIEPLDKYPLAPGVPLPSPQDLMGRILVKNKKRHRPSAGGPD
SAGRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKPSLEPQKSLG
DEGLNRGPYVLGPADREDEEEDEEEEEQTDPKKPTTDEGTASSEVNATEEMSTLVNYIEP
VKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNY
MPQLFWNVGCQLVALNFQTLDVAMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVI
VDGIVANALRVKVISGQFLSDRKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEE
PFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVSLRNEANQPLSLPALL
IYTEASDYIPDDHQDYAEALINPIKHVSLMDQRARQLAALIGE

Sequence of entity 2 (B), FASTA

>9Y7H_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
RQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDS
RLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLK
TREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDV
MSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDD
ATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGV
LAGHDNRVSCLGVTDDGMAVATGSWDSFLKIW

Sequence of entity 3 (G), FASTA

>9Y7H_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	1
ME7	1,1'-ethane-1,2-diylbis(1H-pyrrole-2,5-dione)	C10 H8 N2 O4	1

Primary citation

G beta gamma engages PLC beta 3 at multiple sites to reorient and facilitate its activation. Fisher, I.J., Senarath, K., Outlaw, K. et al. bioRxiv (2026). DOI 10.64898/2026.01.14.699417 · PubMed

Other PDB entries of the same protein (UniProt Q01970 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7SQ2 2.6 Å, Reprocessed and refined structure of Phospholipase C-beta and Gq signaling complex
4QJ3 3.0 Å, Structure of a fragment of human phospholipase C-beta3 delta472-559, in complex with…
4QJ4 3.3 Å, Structure of a fragment of human phospholipase C-beta3 delta472-569, bound to IP3 and in…
8EMX 3.3 Å, Phospholipase C beta 3 (PLCb3) in complex with Gbg on lipid nanodiscs
8UQO 3.37 Å, PLCb3-Gbg-Gaq complex on membranes
8UQN 3.4 Å, PLCb3-Gaq complex on membranes
4QJ5 3.41 Å, Structure of a fragment of human phospholipase C-beta3 delta472-581, bound to IP3 and in…
8EMW 3.5 Å, Phospholipase C beta 3 (PLCb3) in complex with Gbg on liposomes
8EMV 3.6 Å, Phospholipase C beta 3 (PLCb3) in solution
4GNK 4.0 Å, Crystal structure of Galphaq in complex with full-length human PLCbeta3
9YAP 4.5 Å, Gbg crosslinked to PLCb3 - second conformation
9YAO 7.0 Å, Gbg crosslinked to PLCb3 - second conformation

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