9YAO: Gbg crosslinked to PLCb3 - second conformation

Gbg crosslinked to PLCb3 - second conformation. Determined by electron microscopy at 7.0 Å resolution. Released 18 Feb 2026.

Method: Electron microscopy
Resolution: 7.0 Å
Organisms: Homo sapiens, Bos taurus
Chains: 3
Atoms: 8,896
Mol. weight: 144 kDa
Released: 18 Feb 2026

Explore 9YAO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9YAO contains 44 α-helices and 70 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 37 helices, 37 β-strands

Element	Residues	Length	Sheet
α-helix	20-23	4
β-strand	26-29	4	1
β-strand	32	1	2
β-strand	37	1	2
β-strand	40-43	4	1
β-strand	44-45	2	3
β-strand	51-55	5	3
β-strand	61-65	5	3
β-strand	69-74	6	1
α-helix	84-89	6
β-strand	103-108	6	1
β-strand	116-121	6	1
α-helix	127-140	14
α-helix	143-146	4
α-helix	149-162	14
β-strand	170	1	4
α-helix	172-178	7
α-helix	183-192	10
β-strand	203	1	4
α-helix	210-219	10
α-helix	224-233	10
α-helix	243-252	10
α-helix	269-278	10
α-helix	293-301	9
α-helix	303-305	3
α-helix	310-313	4
α-helix	323-325	3
β-strand	326-328	3	5
β-strand	331	1	6
β-strand	336	1	7
β-strand	342	1	8
β-strand	345	1	7
α-helix	347-355	9
β-strand	361-362	2	6
β-strand	365-366	2	9
α-helix	367-369	3
β-strand	376-377	2	9
β-strand	383	1	8
β-strand	387-388	2	9
α-helix	389-398	10
β-strand	410-411	2	6
α-helix	419-432	14
α-helix	441-442	2
α-helix	452-455	4
α-helix	457-459	3
α-helix	578-581	4
α-helix	584-587	4
α-helix	588-593	6
β-strand	598	1	10
α-helix	600-602	3
α-helix	605-611	7
β-strand	616	1	10
α-helix	622-631	10
α-helix	633-642	10
β-strand	647	1	11
α-helix	648-650	3
α-helix	661-665	5
β-strand	672	1	11
α-helix	681-690	10
β-strand	698-700	3	5
α-helix	701-702	2
α-helix	703-706	4
β-strand	728-735	8	12
β-strand	745-752	8	13
α-helix	755-757	3
β-strand	766	1	13
β-strand	775	1	12
β-strand	793-800	8	13
β-strand	804-812	9	13
β-strand	821	1	12
β-strand	826	1	13
β-strand	838-844	7	12
α-helix	869-877	9
α-helix	878-880	3

Chain B: 4 helices, 33 β-strands

Element	Residues	Length	Sheet
α-helix	10-25	16
α-helix	30-35	6
α-helix	38-39	2
α-helix	44-46	3
β-strand	49	1	14
β-strand	52	1	14
β-strand	58-62	5	15
β-strand	69-74	6	15
β-strand	78-83	6	15
β-strand	88-94	7	15
β-strand	112	1	16
β-strand	116	1	17
β-strand	123-124	2	16
β-strand	135-137	3	16
β-strand	146-153	8	17
β-strand	156-161	6	17
β-strand	166-170	5	17
β-strand	175-180	6	17
β-strand	187	1	18
β-strand	191-192	2	19
β-strand	199-201	3	19
β-strand	203	1	18
β-strand	209-211	3	19
β-strand	221	1	19
β-strand	229-234	6	20
β-strand	240-245	6	20
β-strand	250	1	21
β-strand	251-254	4	20
β-strand	259	1	20
β-strand	264	1	21
β-strand	276-278	3	22
β-strand	284-288	5	22
β-strand	293-298	6	22
β-strand	304-309	6	22
β-strand	315-320	6	14
β-strand	327-331	5	14
β-strand	335-338	4	14

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	11-24	14
α-helix	30-45	16
α-helix	55-57	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3	A	protein	883	Homo sapiens	Q01970 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	332	Bos taurus	P62871 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9YAO_1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (chains A)
MAHHHHHHGTALQLEPPTVVETLRRGSKFIKWDEETSSRNLVTLRVDPNGFFLYWTGPNM
CVDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDARLEEKLMTVVSGPDPVNTVFLN
FMAVQDDTAKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMF
SADKKRVETALESSGLKFNRSESIRPDEFSLEIFERFLNKLSLRPDIDKILLEIGAKGKP
YLTLEQLMDFINQKQRDPRLNEVLYPPLRPSQARLLIEKYEPNQQFLERDQMSMEGFSRY
LGGEENGILPLEALDLSTDMTQPLSAYFINSSHNTYLTAGQLAGTSSVEMYRQALLWGSR
CVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAETAFKTSPYPVILSFENHVDSA
KQQAKMAEYCRSIFGDALLIEPLDKYPLAPGVPLPSPQDLMGRILVKNKKRHRPSAGGPD
SAGRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKPSLEPQKSLG
DEGLNRGPYVLGPADREDEEEDEEEEEQTDPKKPTTDEGTASSEVNATEEMSTLVNYIEP
VKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNY
MPQLFWNVGCQLVALNFQTLDVAMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVI
VDGIVANALRVKVISGQFLSDRKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEE
PFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVSLRNEANQPLSLPALL
IYTEASDYIPDDHQDYAEALINPIKHVSLMDQRARQLAALIGE

Sequence of entity 2 (B), FASTA

>9YAO_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
RQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDS
RLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLK
TREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDV
MSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDD
ATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGV
LAGHDNRVSCLGVTDDGMAVATGSWDSFLKIW

Sequence of entity 3 (G), FASTA

>9YAO_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Primary citation

G beta gamma engages PLC beta 3 at multiple sites to reorient and facilitate its activation. Fisher, I.J., Senarath, K., Outlaw, K. et al. bioRxiv (2026). DOI 10.64898/2026.01.14.699417 · PubMed

Other PDB entries of the same protein (UniProt Q01970 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7SQ2 2.6 Å, Reprocessed and refined structure of Phospholipase C-beta and Gq signaling complex
4QJ3 3.0 Å, Structure of a fragment of human phospholipase C-beta3 delta472-559, in complex with…
4QJ4 3.3 Å, Structure of a fragment of human phospholipase C-beta3 delta472-569, bound to IP3 and in…
8EMX 3.3 Å, Phospholipase C beta 3 (PLCb3) in complex with Gbg on lipid nanodiscs
8UQO 3.37 Å, PLCb3-Gbg-Gaq complex on membranes
8UQN 3.4 Å, PLCb3-Gaq complex on membranes
4QJ5 3.41 Å, Structure of a fragment of human phospholipase C-beta3 delta472-581, bound to IP3 and in…
8EMW 3.5 Å, Phospholipase C beta 3 (PLCb3) in complex with Gbg on liposomes
8EMV 3.6 Å, Phospholipase C beta 3 (PLCb3) in solution
4GNK 4.0 Å, Crystal structure of Galphaq in complex with full-length human PLCbeta3
9Y7H 4.4 Å, Gb1g2 crosslinked to PLCb3
9YAP 4.5 Å, Gbg crosslinked to PLCb3 - second conformation

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