Multifunctional conjugation protein TraI (traI) is a 1756-residue protein from Escherichia coli (strain K12). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P14565.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 81.1 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 25% |
| 70 to 90 | Confident: backbone generally right | 61% |
| 50 to 70 | Low: treat with caution | 8% |
| Below 50 | Very low: often disordered regions | 6% |
What pLDDT means and how to read it
Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut at the nic site in the origin of transfer by TraI (PubMed:12637015, PubMed:8386720). Relaxosome formation requires binding of IHF and TraY to the oriT region, which then facilitates binding of TraI relaxase (PubMed:7499339, PubMed:7499340). TraI forms a covalent 5'-phosphotyrosine intermediate linkage with the nicked ssDNA. The trans-esterified single-stranded T-strand moves from the…
Monomer (PubMed:133022, PubMed:2830275). Part of the relaxosome, a complex composed of plasmid-encodes TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of transfer (oriT) (PubMed:7499339, PubMed:7499340). Directly contacts coupling protein TraD. May directly contact TraM via its C-terminus (PubMed:15629940). Forms a dimer on single-stranded (ss) oriT DNA; one monomer is on the…
Cytoplasm
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3FLD | X-ray | 2.4 Å | A/B=1476-1628 |
| 2Q7T | X-ray | 2.42 Å | A/B=1-300 |
| 1P4D | X-ray | 2.6 Å | A/B/C=1-330 |
| 2A0I | X-ray | 2.72 Å | A=1-330 |
| 9F10 | EM | 2.94 Å | H=1-1756 |
| 2Q7U | X-ray | 3.0 Å | A/B=1-300 |
| 9F0Z | EM | 3.42 Å | H=1-863 |
| 9F12 | EM | 3.42 Å | H=1-1756 |
| 9F0Y | EM | 3.45 Å | H=1-1756 |
| 9F11 | EM | 3.68 Å | H=1-1756 |
| 9F0X | EM | 3.78 Å | H=1-1756 |
| 2L8B | NMR | A=381-569 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.