Kinetochore protein SPC25 (SPC25) is a 221-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P40014.
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The mean pLDDT of this model is 89.6 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 67% |
| 70 to 90 | Confident: backbone generally right | 30% |
| 50 to 70 | Low: treat with caution | 3% |
| Below 50 | Very low: often disordered regions | 1% |
What pLDDT means and how to read it
Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity
Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to…
Nucleus, Chromosome, centromere, kinetochore
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5T6J | X-ray | 1.75 Å | B=133-221 |
| 2FTX | X-ray | 1.9 Å | A=133-221 |
| 4GEQ | X-ray | 2.01 Å | A/C=133-221 |
| 7KDF | X-ray | 2.72 Å | D=137-221 |
| 5TCS | X-ray | 2.83 Å | D=137-221 |
| 8V10 | X-ray | 3.02 Å | D=137-221 |
| 8V11 | X-ray | 3.95 Å | D/H=137-220 |
| 5TD8 | X-ray | 7.53 Å | D=1-221 |
| 2FV4 | NMR | A=128-221 |
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