Spindle assembly checkpoint serine/threonine-protein kinase BUB1 (BUB1) is a 1021-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P41695.
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The mean pLDDT of this model is 63.9 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 19% |
| 70 to 90 | Confident: backbone generally right | 34% |
| 50 to 70 | Low: treat with caution | 9% |
| Below 50 | Very low: often disordered regions | 38% |
What pLDDT means and how to read it
Involved in mitotic spindle assembly checkpoint signaling, a process that delays anaphase until chromosomes are bioriented on the spindle, and in the repair of incorrect mitotic kinetochore-spindle microtubule attachments (PubMed:12769845, PubMed:24066227, PubMed:7969164). The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism (PubMed:7969164). Phosphorylates BUB3 (PubMed:7969164). Also autophosphorylates (PubMed:7969164). Associates with centromere (CEN) DNA via interaction with SKP1 (PubMed:12769845). The association with SKP1 is required for the mitotic delay induced by kinetochore tension defects, but not for the arrest induced by spindle…
Part of complex consisting of MAD1, BUB1 and BUB3 after activation of spindle checkpoint (PubMed:10837255). Part of the BUB1-BUB3 complex, composed of BUB1 and BUB3 (PubMed:17227844, PubMed:24066227). Interacts with SPC105 (via phosphorylated MELT motifs); the interaction occurs when part of the BUB1-BUB3 complex (PubMed:22521787, PubMed:24066227). Interacts with SKP1; the interaction is direct…
Nucleus, Chromosome, centromere, kinetochore
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3ESL | X-ray | 1.74 Å | A/B=29-230 |
| 2I3S | X-ray | 1.9 Å | B/D/F=315-350 |
| 4BL0 | X-ray | 1.95 Å | B/E=289-359 |
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