Green fluorescent protein (GFP) is a 238-residue protein from Aequorea victoria. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P42212.
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The mean pLDDT of this model is 96.6 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 93% |
| 70 to 90 | Confident: backbone generally right | 6% |
| 50 to 70 | Low: treat with caution | 1% |
| Below 50 | Very low: often disordered regions | 0% |
What pLDDT means and how to read it
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin
Monomer
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 6L27 | X-ray | 0.77 Å | A=4-231 |
| 6JGJ | X-ray | 0.78 Å | A=2-231 |
| 6KL0 | X-ray | 0.8 Å | A=2-231 |
| 6JGI | X-ray | 0.85 Å | A=2-231 |
| 6KL1 | X-ray | 0.85 Å | A=2-232 |
| 2WUR | X-ray | 0.9 Å | A=1-238 |
| 6KKZ | X-ray | 0.9 Å | A=2-231 |
| 6JGH | X-ray | 0.94 Å | A=2-231 |
| 4AS8 | X-ray | 1.02 Å | A=2-238 |
| 5OXC | X-ray | 1.02 Å | A=2-238 |
| 7PCA | X-ray | 1.05 Å | A=1-238 |
| 4GF6 | X-ray | 1.1 Å | B=1-237 |
| 4LQT | X-ray | 1.1 Å | A=2-238 |
| 4KAG | X-ray | 1.12 Å | A=2-238 |
| 6FWW | X-ray | 1.13 Å | A=2-238 |
| 5DRF | X-ray | 1.14 Å | A=1-238 |
| 5DRG | X-ray | 1.14 Å | A=1-238 |
| 2AWK | X-ray | 1.15 Å | A=2-229 |
| 2WSO | X-ray | 1.15 Å | A=1-238 |
| 6OFK | X-ray | 1.15 Å | A/B=49-237 |
Showing 20 of 654 experimental structures (best resolution first).
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