LIM domain kinase 1 (LIMK1) is a 647-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P53667.
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The mean pLDDT of this model is 75.2 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 31% |
| 70 to 90 | Confident: backbone generally right | 40% |
| 50 to 70 | Low: treat with caution | 11% |
| Below 50 | Very low: often disordered regions | 18% |
What pLDDT means and how to read it
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11832213, PubMed:12807904, PubMed:15660133, PubMed:16230460, PubMed:18028908, PubMed:22328514, PubMed:23633677). Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop (PubMed:10436159). LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and…
Interacts (via LIM domain) with the cytoplasmic domain of NRG1. Interacts with NISCH. Interacts with RLIM and RNF6 (By similarity). Self-associates to form homodimers (PubMed:10196227). Interacts with HSP90AA1; this interaction promotes LIMK1 dimerization and subsequent transphosphorylation (PubMed:16641196). Interacts with CDKN1C (PubMed:14530263). Interacts with SSH1 (PubMed:15660133).…
Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, Cell projection, lamellipodium
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3S95 | X-ray | 1.65 Å | A/B=330-637 |
| 8AAU | X-ray | 1.74 Å | L=330-637 |
| 6WLY | X-ray | 1.9 Å | B=503-512 |
| 5HVJ | X-ray | 2.2 Å | A/B=329-638 |
| 5NXC | X-ray | 2.25 Å | L=330-637 |
| 5L6W | X-ray | 2.53 Å | L=330-637 |
| 7ATS | X-ray | 2.8 Å | A=330-637 |
| 7ATU | X-ray | 2.8 Å | A/B/C/D=330-637 |
| 7B8W | X-ray | 2.8 Å | A/B/C/D=330-637 |
| 5HVK | X-ray | 3.5 Å | A/C=329-638 |
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