RNA polymerase sigma factor RpoD (rpoD) is a 613-residue protein from Escherichia coli. This is its AlphaFold structure prediction, created 1 Jun 2022. UniProt accession: Q0P6L9.
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The mean pLDDT of this model is 82.1 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 45% |
| 70 to 90 | Confident: backbone generally right | 37% |
| 50 to 70 | Low: treat with caution | 11% |
| Below 50 | Very low: often disordered regions | 8% |
What pLDDT means and how to read it
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth
Interacts transiently with the RNA polymerase catalytic core
Cytoplasm
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 2P7V | X-ray | 2.6 Å | B=546-613 |
| 6N61 | X-ray | 3.25 Å | F=1-613 |
| 6N60 | X-ray | 3.68 Å | F=1-613 |
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