Myosin light chain kinase, smooth muscle (MYLK) is a 1914-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q15746.
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The mean pLDDT of this model is 65.9 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 18% |
| 70 to 90 | Confident: backbone generally right | 43% |
| 50 to 70 | Low: treat with caution | 5% |
| Below 50 | Very low: often disordered regions | 35% |
What pLDDT means and how to read it
Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC) (PubMed:11113114). Also regulates actin-myosin interaction through a non-kinase activity (PubMed:11113114). Phosphorylates PTK2B/PYK2 and myosin light-chains (PubMed:11113114, PubMed:18587400). Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma (PubMed:20139351). Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance (By similarity). Necessary…
All isoforms including Telokin bind calmodulin. Interacts with SVIL (By similarity). Interacts with CTTN; this interaction is reduced during thrombin-induced endothelial cell (EC) contraction but is promoted by the barrier-protective agonist sphingosine 1-phosphate (S1P) within lamellipodia. A complex made of ABL1, CTTN and MYLK regulates cortical actin-based cytoskeletal rearrangement critical…
Cytoplasm, Cell projection, lamellipodium, Cleavage furrow, Cytoplasm, cytoskeleton, stress fiber
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 9MXD | X-ray | 1.17 Å | B=1742-1761 |
| 9MVW | X-ray | 1.58 Å | B=1742-1761 |
| 5JQA | X-ray | 1.8 Å | B=1742-1761 |
| 5JTH | X-ray | 1.84 Å | B=1742-1761 |
| 6C6M | X-ray | 2.5 Å | A/B/C=405-507 |
| 2CQV | NMR | A=1238-1338 | |
| 2K0F | NMR | B=1742-1760 | |
| 2YR3 | NMR | A=510-601 |
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